View allAll Photos Tagged focusstacking
Merger of focus-stacked 70 images in Helicon Focus. Processed in Photoshop and DxO Nik Color Efex 4.
Focus-stack of 101 images captured in Helicon Remote and merged in Helcon Focus. Processed in Adobe Lightroom Classic and ON1 Photo Raw 2018 Effects (Dynamic Contrast and Tone Enhancer)
A studio stack of 350 images
focus step 0.004mm
stackshot rail
zerene stacker
Nikon D810
mitutoyo 10x/0.28 microscope objective on a nikon 200mm f4 lens
This little guy came from the drainage of a hot spring in Lassen County, California. The white fuzzy stuff he is resting on is a "Q-Tip", or an equivalent generic brand of swab.
I created this image with my old Nikon D50 DSLR. I used an inexpensive 10X microscope finite objective lens (160mm focal length), mounted on a stack of extension tubes with a Nikon to RMS adapter on the front end of the tube stack. There was no other lens- just the microscope objective- projecting an image directly onto the camera's 23.7 x 15.5mm image sensor.
I used Zerene Stacker to stack together 67 photos, combining them into the one you see here.
Each image was taken with the subject progressively farther from the lens. I used a micrometer stage from an old optical comparator to move the subject a smidgen before each new frame. Each smidgen was calibrated to allow some slight overlap for the depth of field, to prevent banding in the final image.
This allows the extremely shallow (about 12 microns, or 0.00047 inches) DOF to be compensated for, with special software than can "glue together" the individual 12-micron slices of sharp focus into a usable complete image.
The image as seen here was the complete frame from the camera (uncropped). At the 10X magnification of the microscope lens, that means that the subject fits into a frame that is 2.4mm across. So the critter is a hair under 2mm long, in "real life".
Lighting was provided exclusively by a 33-watt compact-fluorescent "circline" bulb in the ceiling of the kitchen where this image was created. Exposure time per frame was 2 seconds.
Click here to read the Wikipedia entry about Springtails.
Focus-stacked image from 20 macro shots. Sony a6000 with 4 macro-rings (2x 16mm macro-rings and 2x 10mm macro-rings).
A fire follower, this was seen the spring following the Park Hill Fire of 2015,
Eastern San Luis Obispo Co., California
A single tulip stands out against the bokeh of the variety of coloured tulips behind. The bokeh quality of the Sony 135mm f/1.8 GMaster is awesome. Combine the bokeh, a focus stack of 15 images and the close focusing capabilities makes this a must have in my bag.
First attempt at focus stacking - after a few failed tries, I learned the hard way that the lastest version of Photoshop (15.1) has a bug and doesnt mask the focus areas when using the Auto Blend layers command.
Aspidimorpha rainori, a new specie from Benin.
This photography was used to describe this beautiful tortoise beetle, with the different known paratypes.
(Coleoptera, Chrysomelidae, Cassidinae)
Studio work, stacking made of 70 images assembled using Zerene Stacker (Pmax & Dmap). Post production using Adobe Photoshop CS6 and LR.
Canon 600D. Schneider Kreuznach Componon S 50mm, ISO-100, f2.8 1/13 sec. 4 daylight LED, diffused with a white plastic cup.
Magnification : 2,6:1
Agrilus cyanescens female
13.VI.2021
- Focus stack of 41-47 images
- Schneider Kreuznach Componon 28 mm f/4 at f/4 reversed on extension tubes
- Olympus OM-D E-M1 Mark II
- High res mode
Agrilus integerrimus male
Body length : 7 mm
20.VII.2021
Bourg-Saint-Maurice, Arc 2000, Savoie, France
Captured on Alnus sp.
- Focus stack of 50-55 images
- Microscope objective (Nikon M Plan 10x 160/0.25)
- Olympus OM-D E-M1 Mark II
- High res mode
This is a composite created by focus stacking 5 images of the same rose with slightly different focus. D300, Sigma 150mm at f8 with polarizer, ISO200, Ringflash with polarizer.
I think I have the ID right for this attractively coloured Psocoptera, this one was quite a willing subject and stayed put for a series of focus stacks. This was a 12 image handheld focus stack of this little one. this I think a later nymph stage and was about 2.5mm in length.
Some Wiki info :-
Psocoptera are an order of insects that are commonly known as booklice, barklice or barkflies. They first appeared in the Permian period, 295–248 million years ago. They are often regarded as the most primitive of the hemipteroids. Their name originates from the Greek word ψωκος, psokos meaning gnawed or rubbed and πτερα, ptera meaning wings. There are more than 5,500 species in 41 families in three suborders. Many of these species have only been described in recent years.
They range in size from 1–10 millimeters (0.04–0.4 in) in length.
The species known as booklice received their common name because they are commonly found amongst old books—they feed upon the paste used in binding. The barklice are found harmlessly on trees, feeding on algae and lichen. No member of this order is currently considered endangered; in fact, in 2007, Atlantopsocus adustus, a species native to Madeira and the Canary Islands, was found to have colonized the mild Cornish coast of southwest England.
In the 2000s, morphological and molecular evidence has shown that the parasitic lice (Phthiraptera) evolved from within the psocopteran suborder Troctomorpha. In modern systematics, Psocoptera and Phthiraptera are therefore treated together in the order Psocodea.
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Der Palpuognasee liegt in den Albula-Alpen auf 1918 m ü. M. oberhalb von Preda im Schweizer Kanton Graubünden.
Agrilus olivicolor
Body length : 5.5 mm
09.VII.2021 - Houyet, Belgium
On Quercus sp.
- Focus stack of 32-39 images
- Olympus 60mm macro
- Olympus OM-D E-M1 Mark II
- High res mode
Found this couple of leafroller weevils (Apoderus coryli, Atellabidae) on Alder near the river Gauja (Latvia, Valmiera, July 2014, excessively hot day, +30°C).
Fieldstack based on 9 images (incomplete). Assembled in Zerene Stacker (Dmap & Pmax) and treated in GIMP to remove artifacts.
Canon 5D mkii, EF 100mm macro 1:2.8 USM + 21mm extension ring, ISO-400, f/9, step -0.3, 1/13s, natural light, late afternoon. Slightly cropped image.
Previous post on this species in the first comment
3-D Cross-eye stereogram . Each pic focus stacked from 5 shots.
stare at pair slightly defocus and cross your eyes until an image appears in the middle and then try to relax your eyes to lock the 3-D image. It's interesting how the 3-D really sorts out this tangle. Slightly harder to visualise than #1- think I used too much separation between shots.