View allAll Photos Tagged Compilation,
Well I took a few shots on Saturday night at the Worcester Charity Fireworks - so rather than bore you with successive uploads I thought I'd put them together!
Check out my greatest hits compilation, and let me know what you think:
www.elliotmcguckenphotography.com/Other/45SURF-Heros-Jour...
As the Great Mick Jagger satted, "Pretty, pretty, pretty, pretty, girl
Pretty, pretty
Such a pretty, pretty, pretty girl." -Beast of Burden Sexy, hot, and cute too!
Epic Goddess Straight Out of Hero's Journey Mythology! Pretty Model! :) Tall, thin, fit and beautiful!
Welcome to your epic hero's journey! The beautiful 45surf goddess sisters hath called ye to adventure, beckoning ye to read deeply Homer's Iliad and Odyssey, whence ye shall learn of yer own exalted artistic path guided by Hero's Journey Mythology. I wouldn't be saying it if it hadn't happened to me.
New 500px!
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New instagram! instagram.com/45surf
Pretty Swimsuit Bikini Model Goddess! :)
Join/like my facebook page! www.facebook.com/45surfHerosJourneyMythology
Follow me on facebook! facebook.com/elliot.mcgucken
Nikon D300 Photos of Beautfiul Sexy Hot Brunette!
She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Journey from Johnny Ranger McCoy!
Sony A7R RAW Photos of Pretty, Tall Blond Bikini Swimsuit Model Goddess in Laguna Beach! Victoria Beach! Carl Zeiss Sony FE 55mm F1.8 ZA Sonnar T* Lens & Lightroom 5.3
New Instagram! instagram.com/45surf
New blog celebrating my philosophy of photography with tips, insights, and tutorials!
Ask me any questions! :)
Sony A7R RAW Photos of Pretty Brunette Bikini Swimsuit Model Goddess! Carl Zeiss Sony FE 55mm F1.8 ZA Sonnar T* Lens! Lightroom 5.3 ! Pretty Hazel Eyes & Silky Brown / Black Hair!
And here're a couple of HD video movies I shot of the goddess with the 4K Sony:
Enjoy! Be sure to watch in the full 1080P HD!
The epic goddess was tall, thin, fit, tan, and in wonderful shape (as you can see).
Check out my greatest hits compilation, and let me know what you think:
www.elliotmcguckenphotography.com/45surf/45SURF-Heros-Ody...
Epic Goddess Straight Out of Hero's Odyssey Mythology! Pretty Model! :) Tall, thin, fit and beautiful!
Welcome to your epic hero's odyssey! The beautiful 45surf goddess sisters hath called ye to adventure, beckoning ye to read deeply Homer's Iliad and Odyssey, whence ye shall learn of yer own exalted artistic path guided by Hero's Odyssey Mythology. I wouldn't be saying it if it hadn't happened to me.
New 500px!
500px.com/herosodysseymythology
New instagram! instagram.com/45surf
Pretty Swimsuit Bikini Model Goddess! :)
Follow me on facebook! facebook.com/elliot.mcgucken
Nikon D300 Photos of Beautfiul Sexy Hot Brunette!
She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Odyssey from Johnny Ranger McCoy!
Modeling the Gold 45 Revolver Gold'N'Virtue swimsuit. :)
A laid-back,classic, socal lifestyle shoot!
May the 45surf goddesses inspire you along am artistic journey of your own making!
All 45surf Hero's Odyssey Mythology Photography is shot in the honor of Truth, Beauty, and the Light of Physicist Dr. E's Moving Dimensions Theory's dx4/dt=ic . The fourth dimension is expanding relative to the three spatial dimensions at the rate of c. Ergo relativity, time, entropy, and entanglement.
All the best on your Epic Hero's Odyssey from Johnny Ranger McCoy!
New blog celebrating my philosophy of photography with tips, insights, and tutorials!
Ask me any questions! :)
Sony A7R RAW Photos of Pretty Brunette Bikini Swimsuit Model Goddess! Carl Zeiss Sony FE 55mm F1.8 ZA Sonnar T* Lens! Lightroom 5.3 ! Pretty Hazel Eyes & Silky Brown Black Hair!
All the best on your Epic, Homeric, Heroic Odyssey into the Art of Photography from Johnny Ranger McCoy!
All 45surf Hero's Odyssey Mythology Photography is shot in the honor of Dynamic Dimensions Theory's First Law and equation: The fourth dimension is expanding relative to the three spatial dimensions at the rate of c: dx4/dt=ic.
Join my new Instagrams:
instagram.com/johnnyrangermccoy
All the best on your Epic Hero's Odyssey from Johny Ranger McCoy!
PAUL McCARTNEY: GOT BACK 2022 VOL.1 DVD
January thru February, 2022! Promotion, ads, interviews, trailers, messages, appearances and more! Got Back Tour is coming!
New Year Instagram story, Wild Life 50th, Exhibit in London, My Valentine - Michael Buble, tour news and ads and more from
Spokane, Seattle, Fort Worth, Winston-Salem, Boston, Knoxville, LA, Oakland, Syracuse, Baltimore, Hollywood, Orlando,
East Rutherford and & much more! SUPER compilation in EX quality throughout!
Approx 100 mins 66 chapters!
01 220101 Paul Portrays Linstrom - Instagram Story
02 220103 Paul's Funny Faces - BBC News
03 Linda McCartney Foods Trailer
04 220104 St Vincent on Paul - James Corden Show
05 220107 Sticking Out Of My Back Pocket Ad
06 220119 McCartney Exhibit in London - Instagram
07 World Mental Health Day 2021 Paul with Melyn Media
08 220121 Paul in car by Abbey Road
09 220123 Flaming Pie Ad
10 220124 Paul Memorabilia & More In MusiCares Charity Auction - KCAL
11 220131 Celebrate 50 years of Wild Life Listening Party
12 220204 Wild Life 50th Anniversary Release 2 ads
13 220205 Wild Life 50th Anniversary Release
14 220210 My Valentine - Michael Buble - Promo Video
15 220212 Slidin' Inspired by the Winter Olympics Trailer
16 220215 The Lyrics’ at The British Library - PM Post
17 Dave Grohl on Paul & R'n'R Hall of Fame - Howard Stern Show
18 220216 Michael Bublé onTeaming Up With Paul On New Album - ET
19 220217 Paul to play Camden Yards MD Rumor - WJZ
20 220218 Got Back Tour ad #1
21 Got Back Tour ad #1
22 Tour Announcement for Baltimore MD - WBAL #1
23 Tour Announcement for Baltimore MD - WBAL #2
24 Tour Announcement for Baltimore MD - WJZ
25 Tour Announcement for Baltimore MD - WMAR #1
26 Tour Announcement for Baltimore MD - WMAR #2
27 Tour Announcement for Baltomore MD - Press Conf
28 Tour Announcement for Boston MA - WBZ
29 Tour Announcement for Hollywood FL - CBS
30 Tour Announcement for Knoxville TN - WATE
31 Tour Announcement for Knoxville TN - WBIR
32 Tour Announcement for Knoxville TN - WJHL
33 Tour Announcement for Knoxville TN - WVLT
34 Tour Announcement for Knoxville TN -WKRN
35 Tour Announcement for LA CA - KCAL
36 Tour Announcement for LA CA - KTLA
37 Tour Announcement for LA CA - WABC
38 Tour Announcement for Orlando FL - WKMG
39 Tour Announcement for Seattle WA - KCPQ
40 Tour Announcement for Seattle WA
41 Tour Announcement for Spokane WA - 4 News
42 Tour Announcement for Spokane WA - KREM #1
43 Tour Announcement for Spokane WA - KREM #2
44 Tour Announcement for Spokane WA - KREM #3
45 Tour Announcement for Syracuse NY - WSYR
46 Tour Announcement for Winston-Salem NC - Journal
47 Tour Announcement for Winston-Salem NC - Queen City
48 Tour Announcement for Winston-Salem NC - WRAL
49 Got Back in Fort Worth, TX Ad #1
50 Got Back in Fort Worth, TX Ad #2
51 Got Back in LA CA - Live Nation
52 Got Back in Winston-Salem NC Ad
53 220219 Got Back kicking off April 28th - Today
54 Paul is coming to Spokane! - KHQ
55 220221 Got Back in LA CA Contest 93.1 Jack FM
56 Paul is coming to Knoxville - WBIR
57 Paul is coming to Spokane - KOIN
58 220222 Avoid scam ticket sellers for Paul concert - KREM
59 220223 Got Back Tour Coming to USA - KCRA
60 Hard Rock Live Hollywood FL Ad #1
61 Got Back Fenway Park Night Two Ad #1
62 Got Back Met Life Ad #1
63 Tickets go on sale today in Spokane - KNDO
64 Tickets on sale today in Winston-Salem - WBIR
65 Second show added in Boston - WWLP
66 Tickets on sale in Fort Worth - CBS
Strobist info:
Camera right, ~45deg: Vivitar 285HV thru umbrella at 1/4 power.
Camera left, ~45deg: 580EX II left bounced of umbrella 1/4
Camera settings: 28mm, F/4 exposure time: 1/200, ISO 200.
Inspired by:
and aknacer
Thanks!
Nikon D800 photos of a Swimsuit Bikini Model Goddess! She's a Hollywood Stunt Double! You've seen her in movies!
And here're a couple of HD video movies I shot of the goddess with the Nikon D800:
Enjoy! Be sure to watch in the full 1080P HD!
The epic goddess was tall, thin, fit, tan, and in wonderful shape (as you can see).
Check out my greatest hits compilation, and let me know what you think:
www.elliotmcguckenphotography.com/45surf/45SURF-Heros-Jou...
As the Great Mick Jagger stated, "Pretty, pretty, pretty, pretty, girl
Pretty, pretty
Such a pretty, pretty, pretty girl." -Beast of Burden Sexy, hot, and cute too!
Epic Goddess Straight Out of Hero's Journey Mythology! Pretty Model! :) Tall, thin, fit and beautiful!
Welcome to your epic hero's journey! The beautiful 45surf goddess sisters hath called ye to adventure, beckoning ye to read deeply Homer's Iliad and Odyssey, whence ye shall learn of yer own exalted artistic path guided by Hero's Journey Mythology. I wouldn't be saying it if it hadn't happened to me.
New 500px!
500px.com/herosjourneymythology
New instagram! instagram.com/45surf
Pretty Swimsuit Bikini Model Goddess! :)
Join/like my facebook page! www.facebook.com/45surfHerosJourneyMythology
Follow me on facebook! facebook.com/elliot.mcgucken
Nikon D300 Photos of Beautfiul Sexy Hot Brunette!
She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Journey from Johnny Ranger McCoy!
Shot with the Nikon D800 and Nikon 70-200mm f/2.8G ED VR II AF-S Nikkor Zoom Lens.
Modeling the Gold 45 Revolver Gold'N'Virtue swimsuit. :)
A laid-back,classic, socal lifestyle shoot!
Reading the Great Books and Classics inclduing Homer, Shakespeare, and Herman Meville's Moby Dick!
With the black 45SURF surfboard!
May the 45surf goddesses inspire you along am artistic journey of your own making!
Tiesto during the TIESTO-Kaleidoscope World Tour performance held at InterContinental Hotels Doha Beach Area in Doha, Qatar, 01 October 2010.
DJ TIESTO live performance at the stage of InterContinental Hotels Doha, Beach Area on October 01, 2010 Doha, Qatar.
Music fans of local and foreign nationalities welcomes back in an up-close-and-personal experience the ever smiling world famous DJ as he performs his best selling music in unison with his own extravagant and jaw-dropping giant LED screen, animated light works and powerful sound system.
TIESTO "Kaleidoscope World Tour" was a success full house brought-in Doha, organized and managed by Jared Mcculloch of Global DJs.
Hotel InterContinental Doha Beach Area is the premier concert venue in Doha.
(Photo and Text by ROMMEL T. BANGIT
Resolution in Mega pixels:
Camera: Nikon D300
12.3 Mega pixels
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(4288 x 2848 px) unless cropped
Resolution: 300 pixels/inch
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Below text source from WIKIPEDIA:
TIESTO also known as DJ Tiësto, Allure, Da Joker, Drumfire, Handover Circuit, Paradise In Dubs, Passenger, Roze, Steve Forte Rio, Stray Dog, Tom Ace, Wild Bunch
Tiësto OON (Dutch pronunciation: [ˈcɛstoː]; born Tijs Michiel Verwest [ˈtɛi̯s miˈxil vərˈʋɛst] on January 17, 1969) is a Dutch musician, DJ and record producer of electronic dance music. Although he has used many aliases in the past, he is best known for his work as DJ Tiësto. On his latest productions, however, he has dropped the "DJ" label and is now known simply as "Tiësto", an alias which is a twist of his childhood nickname.
In 1997, he founded the label Black Hole Recordings with Arny Bink, where he released the Magik and In Search of Sunrise CD series. In 1999 and 2000 he collaborated with Ferry Corsten to create Gouryella. His 2000 remix of Delerium's "Silence" featuring Sarah McLachlan exposed him to more mainstream audiences. In 2001 he released his first solo album In My Memory which gave him several major hits that launched his career. He was named "World’s No.1 DJ" 3 consecutive times by DJ Magazine from 2002 through 2004.
Just after releasing his second studio album Just Be in 2004 at the Summer Olympics, he performed live at the opening ceremony in Athens, Greece, becoming the first DJ to play live on stage at an Olympics. Tracks he made especially for the Olympics were mixed together and released as the mix compilation Parade of the Athletes later that year. In April 2007 Tiësto launched both his radio show Tiësto's Club Life on Radio 538 in the Netherlands and released his third studio album titled Elements of Life. The album reached number one on the Dutch album chart as well on "Billboard Top Electronic Albums" in the U.S. and received a nomination for a Grammy Award in 2008. Tiësto released his fourth studio album called Kaleidoscope in October 2009.
Early Life and Career
Tijs Michiel Verwest was born in Breda, North Brabant, Netherlands on January 17, 1969. He had an interest in music since the age of twelve. When he was fourteen he decided to dedicate more time to it and began DJing professionally at school parties. He then moved on to become a resident DJ between 1985 and 1994 at several clubs in the Netherlands after his manager and friend Wilfred encouraged him. However, it was at The Spock, a small club in Breda, where he was able to fine-tune his own style by playing in a separate room from 10 p.m. until 4 a.m. on weekends. In the beginning of his career, as a DJ he played mostly New Beat and acid house.
In 1994, he began releasing material on Noculan Records' sub-labels Chemo and Coolman. During these years, he produced hardcore/gabber tracks under aliases such as Da Joker and DJ Limited. He was later discovered by the general manager of Rotterdam-based Basic Beat Recordings. In late 1994, he signed to Basic Beat where he met Arny Bink, Tiësto released records on the sub-label Trashcan, founded by Arny, and later created the Guardian Angel sub-label with Arny in which they introduced the popular Forbidden Paradise series. Meanwhile from 1995 to 1996 he released four extended plays on Bonzai Jumps and XTC, sub-labels of Lightning Records. In 1997, Tiësto joined his friend Yves Vandichel on his sub-label, DJ Yves, a division of the now defunct Human Resource label XSV Music. In the fall of 1997, both Arny and Tiësto decided to leave Basic Beat and create their own parent label, now known as Black Hole Recordings, Trashcan was discontinued and Guardian Angel continued releasing music until 2002. Through Black Hole, Tiësto released the Magik series and also created two major sub-labels in 1998; In Trance We Trust and SongBird.
From 1998 to 1999, Tiësto released music on Planetary Consciousness were he met A&R Hardy Heller and invited him to release some records on Black Hole. Tiësto later included the In Search of Sunrise series on SongBird. In 1999, Tiësto joined forces with fellow Dutch deejay Ferry Corsten to create the trance based duo of Gouryella. He also collaborated with Benno de Goeij from 1998 to 2000 under the name Kamaya Painters. Since November 1999 he performed monthly as a resident at Gatecrasher in Sheffield, one of the most popular clubs in England. In 1999 he also played in a 12-hour set, being his longest lasting concert in Amsterdam.
Late in 2000, Tiësto decided to concentrate on his personal work and left Corsten by himself to write and produce Gouryella's next single with John Ewbank, the record company was demanding more tracks and neither Tiësto or Ferry could work together at the time. Tiësto introduced Armin van Buuren, Johan Gielen and Ferry Corsten to the mainstream with his first compilations and the In Trance We Trust series. Summerbreeze became Tiësto's debut DJ mix album in the US with the help of a contract signed to Nettwerk. Summerbreeze featured his remix of Delerium's "Silence", it spent four weeks in the UK's Top Ten chart, it reached number three in the Billboard dance chart. In Search of Sunrise 2 was released in 2000. Tiësto decided to create a sub-label, known as Magik Muzik. The label began releasing Tiësto’s own releases, but it has also released tracks for the Filterheadz, Oliver Lieb, Mark Norman, Mojado, Phynn and Jes Brieden. The label became a trademark which stands for high quality electronic dance music which was due to the release of Tiësto's classic dance anthem "Flight 643" in 2001.
In My Memory era (2001–2004)
Tiësto's fame started to rise in the early 2000s after his set at the first ID&T Innercity party (Live at Innercity: Amsterdam RAI), and the release of In My Memory, his first solo album released in 2001 which contained 10 singles and 5 major hits; The singles from the album were: "Lethal Industry" which was actually produced in 1999 and had only 3 copies released at that time, the track was officially released in 2001 which was remixed by Richard Durand in 2006 along with "Flight 643" which was another leading single that was later adapted with vocals by Suzanne Palmer and released as "643 (Love's on Fire)". Other tracks were "Obsession" in which Tiësto worked alongside Junkie XL, the instrumental tracks "Dallas 4PM" and "Suburban Train" with "Urban Train" as its B-Side which contained some vocals. The last singles to be released were "In My Memory" which is the title track for the album as it only received high ratings in the United States and the opening track "Magik Journey" which opened Tiësto in Concert (2003). On February 2, 2002 Tiësto played nine consecutive hours during the second edition of the Dutch Dimension festival. On February 27 he was awarded a Zilveren ('Silver') Harp music award. The same year he also received a Lucky Strike Dance Award in the category Best DJ Trance/Progressive. In August he became part of Moby's Area2 Tour. For eighteen days he travelled through the United States with artists such as Moby himself, but also David Bowie and Busta Rhymes. In January 2003 Tiësto received the annual Dutch Popprijs ('Pop Award') during the Noorderslag festival. After touring with Moby, Tiësto remixed two songs from him, "We Are All Made of Stars" and "Extreme Ways" in the same year, having "We Are All Made of Stars" reach #13 in the Hot Dance Club Play. In 2002 he released his first In Search of Sunrise mix to feature a place on its name, In Search of Sunrise 3: Panama. On March 28, 2003; Tiësto, Dieselboy, Bad Boy Bill, and Noel Sanger joined the PlayStation2 Dual Play tour. Tiësto and Noel's appearance began on April 13 and ended on June 6.
His fame continued to skyrocket in the early 2000s, following his six-hour "Tiësto Solo" sets which he performed without other DJs or opening acts. This idea, of one DJ playing alone to a large crowd, was brought to its pinnacle when Tiësto was the first DJ to hold a solo concert in a stadium; on May 10, 2003, he performed for over 25,000 people in Arnhem's GelreDome. This concert was later called Tiësto in Concert, the event was an enormous success. He repeated the same type of concert the following year during two consecutive nights in late October. In addition to holding these two concerts for 35,000 of his fans, he held another concert for a crowd of 20,000 in Hasselt, Belgium the following week. DVDs of both his May 10, 2003 and October 30, 2004 concerts have been released, having the other DVD titled Tiësto in Concert 2. The DVD's show the journey from the first idea to the main event, it features live performances by Andain, Dinand Woesthoff, and Jan Johnston. The event includes live music and dancers performing at different times throughout the set. The theme of the event is a mystical, musical journey around the world based on the theme of Magik. It consists of 200+ minutes of performances with a second disc with special features, It includes a behind-the-scenes looking at The Making Of the event, the music video for his song "Traffic" and TV Commercials for the event. The second DVD has performances from Aqualung and violin player DJ Mason, Micha Klein and the Bulgarian Children of Orpheus choir. During this period, he was crowned as "No. 1. DJ in the World" by DJ Magazine (UK) in 2002, 2003, and 2004.
Just Be era (2004–2007)
In 2004, he released his second artist album Just Be, which featured his first single "Traffic" which is the first non-vocal track to reach number one spot in the Dutch national charts for 23 years. The track "Sweet Misery" was originally written for Evanescence but it did not meet the deadline for the release of their album. In support to his Just Be album, he played at Breda, Eindhoven, Utrecht, and Amsterdam; these stops were later named Just Be: Train Tour. On May 20, 2004 he was appointed Officer of the Order of Orange-Nassau by Her Majesty Queen Beatrix of the Netherlands.
The Athens Organizing Committee for the Olympic Games (ATHOC) asked Tiësto to perform at the Olympic Games, making him the first DJ to play live on stage at an Olympic Games at the 2004 Summer Olympics opening ceremony in Athens, where he played for 90 minutes. Tiësto flew to Athens in January 2004 to have a meeting with the ATHOC. His Tiësto in Concert DVD caught their attention, after which he was asked to write more tracks based on his opening tune "Adagio for Strings" which could fit in with the Olympic spirit and combine the classical with the modern age; They also requested that he play his own produced music. The first rehearsal was on Saturday August, 7, for an empty stadium; the second rehearsal was on Sunday August 8 with 35,000 volunteers, a lot of the people recognised some tracks like "Traffic" and "Adagio for Strings". The last rehearsal included almost 60,000 people in the stadium which was on Tuesday August 10, there were some technical problems, the mixer broke down, the monitors dropped out a couple of times and the music in the stadium was not continuously on the right volume.
During the parade on Friday, August 13, 2004, all participating nations introduced their athletes—10,500 in total There were 80,000 spectators, only 75,000 knowing about dance music. During the course of his performance the Dutch athletes started dancing in front of the DJ booth and had to be moved on by officials. The performance included new tracks produced especially for the Opening Ceremony and songs that were created to complement the spirit and theme of the ceremony. A condensed studio-recorded album of the songs played on the Olympic set was later released, including new songs specially composed for the occasion, entitled Parade of the Athletes in October 2004. In the liner notes, he noted the IOC requested that the music not contain any lyrics as they could be inadvertently misinterpreted.
In late 2004, he began his touring across Latin America, with his release of In Search of Sunrise 3: Panama in which he gained influence from the sun and sand in summer 2002. The tour continued in 2005, and Tiësto performed live at Brazil, Argentina, Panama, Peru, Costa Rica, Uruguay, Paraguay, Ecuador and Colombia. Following the tours, In Search of Sunrise 4: Latin America was released in 2005, featuring a second CD for the first time in the In Search of Sunrise series.
In 2005, his Perfect Remixes Vol. 3 compilation was released through Warlock Records, containing ten tracks which were created during the beginning of his career, between those is Junkie XL, Mauro Picotto and The Roc Project. On August 20, 2005, Verwest took Tiësto in Concert to the US when he played to 16,000 at the Los Angeles Memorial Sports Arena with Cirque du Soleil dancers. For the second year in a row he performed live at a New Year's Eve/New Year's concert in Las Vegas, Nevada at the Orleans Arena to a sell-out crowd. Despite his four-city US tour being postponed due to the hurricane damage in New Orleans and Miami, playing such cities further expanded and cemented his popularity among mainstream audiences. This was surpassed later in summer 2007 when some 250,000 danced on Ipanema Beach, Brazil, becoming the second largest concert in history. BPM magazine has an annual poll in the US which is unveiled in the WMC, in 2005 Tiësto took the No. 1 spot. The influences of Los Angeles remained with him and would later influence his In Search of Sunrise compilation.
A wax sculpture of Tiësto was placed behind a turntable at Madame Tussauds in Amsterdam where visitors can mix Tiësto's music together. In the fall of 2005 he went on a very successful tour across Central and Eastern Europe where he played once in each country to crowds of 10,000 to 15,000 fans. Stops were made in Ukraine, Slovakia, Serbia, Macedonia, Romania, Hungary, the Czech Republic, Turkey, Croatia, Poland and South Africa. The United States tour that was part of Tiësto in Concert was dwarfed by his appearance at Sensation White in 2006 where he performed to over 45,000 people in the world's biggest dance event in Amsterdam, Holland.
In Search of Sunrise 5: Los Angeles was released in 2006 which was certified Gold in Canada for sales over 50,000 copies. It also charted, peaking #34 in Canada and #59 in Austria. The compilation was launched in the Winter Music Conference at Mansion in South Beach Miami to support his release, Tiësto went on his In Search of Sunrise 5 Asia Tour for more than 3 weeks.
In September 2006, Tiësto was admitted to hospital after experiencing pain in his chest. He was diagnosed with pericarditis and subsequently had to cancel a number of shows. With the diagnosis, he was invited to support Dance4Life to help teens who are not aware of the risks of HIV/AIDS. He was chosen as the official ambassador for the Dance4Life foundation in May 2006, and released the song "Dance4Life" with Maxi Jazz to help spread the awareness of HIV/AIDS.
On April 6, 2007 Tiësto began presenting a new weekly two-hour radio show called Tiësto's Club Life on Dutch radio station Radio 538. It is syndicated worldwide and later released as a Podcast.
Elements of Life era (2007-2009)
On April 16, 2007, Tiësto released his third studio album Elements of Life, the album moved 73,000 units in its April release, according to Nielsen SoundScan. During the production of the album Tiësto in several cases sent a demo with the music to certain artists, and they replied back with the lyrics and vocals and other duration times. In the case of Christian Burns from BBMak, Tiësto met him through MySpace and got in contact with him and the production of the single "In the Dark". The album consists of rock, trance and experimental music, which shows the style Tiësto has grown throughout the years since his previous albums which contained lyrics, In My Memory and Just Be. Producer Brian Transeau collaborated with Tiësto in three tracks, he composed "Bright Morningstar" and "Sweet Things", he also performed the vocals in the single "Break My Fall". Together, they produced more tracks which were not released in the album, Tiësto has mentioned they would work again during the coming summer. In December 2007 it was announced that the album was nominated for a Grammy Award, in the category "Best Electronic/Dance Album." The album also received gold certifications in Belgium, Hungary, Netherlands, and Romania.[28] A special release party was held at the Heineken Music Hall in Amsterdam on November 3, 2007 for In Search of Sunrise 6: Ibiza.
His last three full-length releases broke the 70,000 mark, and the 2003 2CD compilation Nyana recently hit 87,000, according to Nielsen SoundScan. In support of the album, he embarked on his Elements of Life World Tour which had shows across the world. Tiësto's performance at Parken Stadium in Copenhagen, Denmark on November 10, 2007 was also sold out.[30] The Copenhagen: Elements of Life World Tour DVD was released in a party which was held on February 29, 2008 in London at the IndigO2 club.
Tiësto announced his residence at Privilege, officially recognised by the Guinness World Records as being the largest club in the world. He played in Ibiza every Monday, from July 7 until September 22. The gigs consisted of sets in the style of his In Search of Sunrise series. In 2007, he had released In Search of Sunrise 6: Ibiza which was inspired by the island. The residencies also featured the performance of guest deejays, all selected by Tiësto, such as Chris Lake, Andy Duguid, Mat Zo, Cosmic Gate, Alex Kunnari and Sander van Doorn as well as exclusive appearances by Fonzerelli and Airbase.
On April 28, Tiësto released Elements of Life: Remixed, a recompilation of the Elements of Life album with all songs being replaced by remixed versions. In mid-2008, Tiësto announced his In Search of Sunrise: Summer Tour 2008, which will be presented by Armani Exchange on May in support of his upcoming In Search of Sunrise 7: Asia compilation[34] and the previously released In Search of Sunrise 6: Ibiza. On August 8, 2008 Tiësto became the first DJ to perform at the famous O2 Arena in London as part of his In Search of Sunrise 7 summer tour, the event was sold out with a capacity of 20,000 people. Earlier that day, Tiësto had performed in-store at Armani Exchange on Regent Street in central London.
A sole production was prepared for Tiësto from June 8 to September 21 for his return at club Privilege for the second year in a row on Monday nights, after a successful year as a resident in 2008. During his time at Privilege he previewed tracks from his upcoming artist album. InTheBooth, the official members-only fansite of Tiësto launched July 17, 2009. On July 31, he was the first DJ to perform for 25,000 people at an exclusive outdoor concert in Victoria Park, London.
Kaleidoscope era (2009–present)
On October 6, 2009, Tiësto released his fourth studio album Kaleidoscope, which featured artists such as Priscilla Ahn, Calvin Harris, Tegan & Sara and Nelly Furtado. Unlike his earlier albums, which were all mostly trance, Kaleidoscope explores other electronic genres, and is considered Tiesto's most experimental album. The first single "I Will Be Here" featuring Sneaky Sound System being released in July 2009.[37][38][39] In its first week, the album reached the Top 10 chart on iTunes.
To release the album he has set up a new record label called Musical Freedom after parting ways with Black Hole Recordings. Tiësto felt that his music was evolving in a new direction and his focus as an artist was moving away from what Black Hole was set up to support. His new tour, sharing the name of his new album, called Kaleidoscope World Tour commenced in late September.
Tiësto contributed songs to both DJ Hero and DJ Hero 2 video games and is a playable character in the second game. He contributed two exclusive mixes to the second game, and used content from DJ Hero 2 to create the official video for his single 'Speed Rail' - the world's first music video to be created entirely using in-game footage.
Tiësto has produced a trance-flavoured song on Memphis rap duo Three 6 Mafia's upcoming album Laws of Power called "Feel It," which features Flo Rida and Sean Kingston.
On March 16, 2010, Tiësto released his greatest hits album called Magikal Journey: The Hits Collection 1998-2008, a two disc album focusing on his most famous songs and remixes of his songs.
On April 7, 2010, Tiësto announced that he would start a new compilation series called A New Dawn with his own label Musical Freedom. In his interview Tiësto furthermore confirmed that he will no longer have any more involvement with Black Hole Recordings.
On August 31, 2010 Kaleidoscope: Remixed was released, a remix album of his album Kaleidoscope.
On December 11, 2010, Tiësto was one of the headlining acts at ZoukOut 2010,[48] which was held in Singapore at Siloso Beach, Sentosa Island.
Philanthropy
On January 6, 2005, Tiësto performed in an outdoor fundraiser in De Dam, Amsterdam. The event was free, and many famous Dutch artists like Dinand Woesthoff, BLØF, Acda & De Munnik, Di-rect, Berget Lewis, Xander de Buisonjé and Trijntje Oosterhuis were involved in it to provide financial aid to the people who suffered from the 2004 Indian Ocean earthquake and tsunami in Southern and Southeastern Asia. All profits made of all TV commercials and live broadcast were given to the organisations collecting the relief funds.
In April 2006, Tiësto was named the official worldwide ambassador for the Dance4Life foundation promoting awareness of HIV/AIDS, as the foundation's ambassador he has helped the organisation with fundraising along with recording the track "Dance4life" that he recorded with Maxi Jazz from Faithless. The foundation consists on a better way of living with safe sex in exchange of entertainment to the young crowd.[50] The song was a huge success, peaking for five weeks in #3 and eleven consecutive weeks in the Top 10 of the Dutch Singles Chart, it also reached #5 in Belgium, #6 in Finland and also charting in the UK and Germany. With the successful release of Elements of Life, Tiësto and fashion designer Giorgio Armani collaborated together on a limited edition Tiësto T-shirt available at Armani Exchange stores. His single "Sweet Things" comes with the shirt including an exclusive "A|X Remix" by Tom Cloud which shows the great influence Tiësto has in fashion culture.[51] The charity raised over US$300,000.[34] On November 29, 2008 artists like Tiësto himself, Sied van Riel, Leon Bolier, Joop, and MC Gunner presented a concert at The Sand, Amsterdam promoted by Dance4Life, the sales from the event will go to the foundation to support next year's Schools4Life project.
Personal life
Until early 2006, Tiësto was in a relationship with Dutch model Monique Spronk. On January 10, 2008, Dutch newspaper De Telegraaf announced that Tiësto and his girlfriend Stacey Blokzijl were going to get married on October 10, 2008 in Cartagena, Colombia. He proposed to her in December 2007 while they were visiting Mauritius. Tiësto cancelled his wedding for October 10, because he claimed to have a busy schedule and too little time for the preparations. During Christmas 2008, Blokzijl broke off the engagement due to the constant delays to their wedding.
Source: Wikipedia
TIESTO, hotel intercontinental hotels and resorts, kaleidoscope,Hotel "InterContinental Hotels and Resorts Doha" Kaleidoscope TIESTO rommelbangit rommelbangit@yahoo.com "electronic dance music" "global DJ" "Nikon D300" trance clubbing dancing lights music musician nightlife party progreesive remix strobe "Top 100 DJ's" "doha qatar" "club culture" "trance sounds" pioneer "US Dance Chart" "Radio 538" "Trance DJ Netherlands" "Digital Spy" "DJ Mag" "DJ Magazine" "Music News" MixMag "Trance Artist" "Progressive Trance" electronic electronix
World leader, scientist, medical scientist, virologist, pharmacist, Professor Fangruida (F.D Smith) on the world epidemic and the nemesis and prevention of new coronaviruses and mutant viruses (Jacques Lucy) 2021v1.5)
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The Nemesis and Killer of New Coronavirus and Mutated Viruses-Joint Development of Vaccines and Drugs (Fangruida) July 2021
*The particularity of new coronaviruses and mutant viruses*The broad spectrum, high efficiency, redundancy, and safety of the new coronavirus vaccine design and development , Redundancy and safety
*New coronavirus drug chemical structure modification*Computer-aided design and drug screening. *"Antiviral biological missile", "New Coronavirus Anti-epidemic Tablets", "Composite Antiviral Oral Liquid", "New Coronavirus Long-acting Oral Tablets", "New Coronavirus Inhibitors" (injection)
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(World leader, scientist, medical scientist, biologist, virologist, pharmacist, FD Smith) "The Nemesis and Killer of New Coronavirus and Mutated Viruses-The Joint Development of Vaccines and Drugs" is an important scientific research document. Now it has been revised and re-published by the original author several times. The compilation is published and published according to the original manuscript to meet the needs of readers and netizens all over the world. At the same time, it is also of great benefit to the vast number of medical clinical drug researchers and various experts and scholars. We hope that it will be corrected in the reprint.------Compiled by Jacques Lucy in Geneva, August 2021
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According to Worldometer's real-time statistics, as of about 6:30 on July 23, there were a total of 193,323,815 confirmed cases of new coronary pneumonia worldwide, and a total of 4,150,213 deaths. There were 570,902 new confirmed cases and 8,766 new deaths worldwide in a single day. Data shows that the United States, Brazil, the United Kingdom, India, and Indonesia are the five countries with the largest number of new confirmed cases, and Indonesia, Brazil, Russia, South Africa, and India are the five countries with the largest number of new deaths.
The new coronavirus and delta mutant strains have been particularly serious in the recent past. Many countries and places have revived, and the number of cases has not decreased, but has increased.
, It is worthy of vigilance. Although many countries have strengthened vaccine prevention and control and other prevention and control measures, there are still many shortcomings and deficiencies in virus suppression and prevention. The new coronavirus and various mutant strains have a certain degree of antagonism to traditional drugs and most vaccines. Although most vaccines have great anti-epidemic properties and have important and irreplaceable effects and protection for prevention and treatment, it is impossible to completely prevent the spread and infection of viruses. The spread of the new crown virus pneumonia has been delayed for nearly two years. There are hundreds of millions of people infected worldwide, millions of deaths, and the time is long, the spread is widespread, and billions of people around the world are among them. The harm of the virus is quite terrible. This is well known. of. More urgent
What is more serious is that the virus and mutant strains have not completely retreated, especially many people are still infected and infected after being injected with various vaccines. The effectiveness of the vaccine and the resistance of the mutant virus are worthy of medical scientists, virologists, pharmacologists Zoologists and others seriously think and analyze. The current epidemic situation in European and American countries, China, Brazil, India, the United States, Russia and other countries has greatly improved from last year. However, relevant figures show that the global epidemic situation has not completely improved, and some countries and regions are still very serious. In particular, after extensive use of various vaccines, cases still occur, and in some places they are still very serious, which deserves a high degree of vigilance. Prevention and control measures are very important. In addition, vaccines and various anti-epidemic drugs are the first and necessary choices, and other methods are irreplaceable. It is particularly important to develop and develop comprehensive drugs, antiviral drugs, immune drugs, and genetic drugs. Research experiments on new coronaviruses and mutant viruses require more rigorous and in-depth data analysis, pathological pathogenic tissues, cell genes, molecular chemistry, quantum chemistry, etc., as well as vaccine molecular chemistry, quantum physics, quantum biology, cytological histology, medicinal chemistry, and drugs And the vaccine’s symptomatic, effectiveness, safety, long-term effectiveness, etc., of course, including tens of thousands of clinical cases and deaths and other first-hand information and evidence. The task of RNA (ribonucleic acid) in the human body is to use the information of our genetic material DNA to produce protein. It accomplishes this task in the ribosome, the protein-producing area of the cell. The ribosome is the place where protein biosynthesis occurs.
Medicine takes advantage of this: In vaccination, artificially produced mRNA provides ribosomes with instructions for constructing pathogen antigens to fight against—for example, the spike protein of coronavirus.
Traditional live vaccines or inactivated vaccines contain antigens that cause the immune system to react. The mRNA vaccine is produced in the cell
(1) The specificity of new coronaviruses and mutant viruses, etc., virology and quantum chemistry of mutant viruses, quantum physics, quantum microbiology
(2) New crown vaccine design, molecular biology and chemical structure, etc.
(3) The generality and particularity of the development of new coronavirus drugs
(4) Various drug design for new coronavirus pneumonia, medicinal chemistry, pharmacology, etc., cells, proteins, DNA, enzyme chemistry, pharmaceutical quantum chemistry, pharmaceutical quantum physics, human biochemistry, human biophysics, etc.
(5) The evolution and mutation characteristics of the new coronavirus and various mutant viruses, the long-term nature, repeatability, drug resistance, and epidemic resistance of the virus, etc.
(6) New coronavirus pneumonia and the infectious transmission of various new coronaviruses and their particularities
(7) The invisible transmission of new coronavirus pneumonia and various mutant viruses in humans or animals, and the mutual symbiosis of cross infection of various bacteria and viruses are also one of the very serious causes of serious harm to new coronaviruses and mutant viruses. Virology, pathology, etiology, gene sequencing, gene mapping, and a large number of analytical studies have shown that there are many cases in China, the United States, India, Russia, Brazil, and other countries.
(8) For the symptomatic prevention and treatment of the new coronavirus, the combination of various vaccines and various antiviral drugs is critical.
(9) According to the current epidemic situation and research judgments, the epidemic situation may improve in the next period of time and 2021-2022, and we are optimistic about its success. However, completely worry-free, it is still too early to win easily. It is not just relying on vaccination. Wearing masks to close the city and other prevention and control measures and methods can sit back and relax, and you can win a big victory. Because all kinds of research and exploration still require a lot of time and various experimental studies. It is not a day's work. A simple taste is very dangerous and harmful. The power and migratory explosiveness of viruses sometimes far exceed human thinking and perception. In the future, next year, or in the future, whether viruses and various evolutionary mutation viruses will re-attack, we still need to study, analyze, prevent and control, rather than being complacent, thinking that the vaccine can win a big victory is inevitably naive and ridiculous. Vaccine protection is very important, but it must not be taken carelessly. The mutation of the new crown virus is very rampant, and the cross-infection of recessive and virulent bacteria makes epidemic prevention and anti-epidemic very complicated.
(10) New crown virus pneumonia and the virus's stubbornness, strength, migration, susceptibility, multi-infectiousness, and occult. The effectiveness of various vaccines and the particularity of virus mutations The long-term hidden dangers and repeated recurrences of the new coronavirus
(11) The formation mechanism and invisible transmission of invisible viruses, asymptomatic infections and asymptomatic infections, asymptomatic transmission routes, asymptomatic infections, pathological pathogens. The spread and infection of viruses and mutated viruses, the blind spots and blind spots of virus vaccines, viral quantum chemistry and
The chemical and physical corresponding reactions at the meeting points of highly effective vaccine drugs, etc. The variability of mutated viruses is very complicated, and vaccination cannot completely prevent the spread of infection.
(12) New crown virus pneumonia and various respiratory infectious diseases are susceptible to infections in animals and humans, and are frequently recurring. This is one of the frequently-occurring and difficult diseases of common infectious diseases. Even with various vaccines and various antiviral immune drugs, it is difficult to completely prevent the occurrence and spread of viral pneumonia. Therefore, epidemic prevention and anti-epidemic is a major issue facing human society, and no country should take it lightly. The various costs that humans pay on this issue are very expensive, such as Ebola virus, influenza A virus,
Hepatitis virus,
Marburg virus
Sars coronavirus, plague, anthracnose, cholera
and many more. The B.1.1.7 mutant virus that was first discovered in the UK was renamed Alpha mutant virus; the B.1.351 that was first discovered in South Africa was renamed Beta mutant virus; the P.1 that was first discovered in Brazil was renamed Gamma mutant virus; the mutation was first discovered in India There are two branches of the virus. B.1.617.2, which was listed as "mutated virus of concern", was renamed Delta mutant virus, and B.1.617.1 of "mutated virus to be observed" was renamed Kappa mutant virus.
However, experts in many countries believe that the current vaccination is still effective, at least it can prevent severe illness and reduce deaths.
Delta mutant strain
According to the degree of risk, the WHO divides the new crown variant strains into two categories: worrying variant strains (VOC, variant of concern) and noteworthy variant strains (VOI, variant of interest). The former has caused many cases and a wide range of cases worldwide, and data confirms its transmission ability, strong toxicity, high power, complex migration, and high insidious transmission of infection. Resistance to vaccines may lead to the effectiveness of vaccines and clinical treatments. Decrease; the latter has confirmed cases of community transmission worldwide, or has been found in multiple countries, but has not yet formed a large-scale infection. Need to be very vigilant. Various cases and deaths in many countries in the world are related to this. In some countries, the epidemic situation is repeated, and it is also caused by various reasons and viruses, of course, including new cases and so on.
At present, VOC is the mutant strain that has the greatest impact on the epidemic and the greatest threat to the world, including: Alpha, Beta, Gamma and Delta. , Will the change of the spur protein in the VOC affect the immune protection effect of the existing vaccine, or whether it will affect the sensitivity of the VOC to the existing vaccine? For this problem, it is necessary to directly test neutralizing antibodies, such as those that can prevent the protection of infection. Antibodies recognize specific protein sequences on viral particles, especially those spike protein sequences used in mRNA vaccines.
(13) Countries around the world, especially countries and regions with more severe epidemics, have a large number of clinical cases, severe cases, and deaths, especially including many young and middle-aged patients, including those who have been vaccinated. The epidemic is more complicated and serious. Injecting various vaccines, taking strict control measures such as closing the city and wearing masks are very important and the effect is very obvious. However, the new coronavirus and mutant viruses are so repeated, their pathological pathogen research will also be very complicated and difficult. After the large-scale use of the vaccine, many people are still infected. In addition to the lack of prevention and control measures, it is very important that the viability of the new coronavirus and various mutant viruses is very important. It can escape the inactivation of the vaccine. It is very resistant to stubbornness. Therefore, the recurrence of new coronavirus pneumonia is very dangerous. What is more noteworthy is that medical scientists, virologists, pharmacists, biologists, zoologists and clinicians should seriously consider the correspondence between virus specificity and vaccine drugs, and the coupling of commonality and specificity. Only in this way can we find targets. Track and kill viruses. Only in this sense can the new crown virus produce a nemesis, put an end to and eradicate the new crown virus pneumonia. Of course, this is not a temporary battle, but a certain amount of time and process to achieve the goal in the end.
(14) The development and evolution of the natural universe and earth species, as well as life species. With the continuous evolution of human cell genes, microbes and bacterial viruses are constantly mutated and inherited. The new world will inevitably produce a variety of new pathogens.
And viruses. For example, neurological genetic disease, digestive system disease, respiratory system disease, blood system disease, cardiopulmonary system disease, etc., new diseases will continue to emerge as humans develop and evolve. Human migration to space, space diseases, space psychological diseases, space cell diseases, space genetic diseases, etc. Therefore, for the new coronavirus and mutated viruses, we must have sufficient knowledge and response, and do not think that it will be completely wiped out.
, And is not a scientific attitude. Viruses and humans mutually reinforce each other, and viruses and animals and plants mutually reinforce each other. This is the iron law of the natural universe. Human beings can only adapt to natural history, but cannot deliberately modify natural history.
Active immune products made from specific bacteria, viruses, rickettsiae, spirochetes, mycoplasma and other microorganisms and parasites are collectively called vaccines. Vaccination of animals can make the animal body have specific immunity. The principle of vaccines is to artificially attenuate, inactivate, and genetically attenuate pathogenic microorganisms (such as bacteria, viruses, rickettsia, etc.) and their metabolites. Purification and preparation methods, made into immune preparations for the prevention of infectious diseases. In terms of ingredients, the vaccine retains the antigenic properties and other characteristics of the pathogen, which can stimulate the body's immune response and produce protective antibodies. But it has no pathogenicity and does not cause harm to the body. When the body is exposed to this pathogen again, the immune system will produce more antibodies according to the previous memory to prevent the pathogen from invading or to fight against the damage to the body. (1) Inactivated vaccines: select pathogenic microorganisms with strong immunogenicity, culture them, inactivate them by physical or chemical methods, and then purify and prepare them. The virus species used in inactivated vaccines are generally virulent strains, but the use of attenuated attenuated strains also has good immunogenicity, such as the inactivated polio vaccine produced by the Sabin attenuated strain. The inactivated vaccine has lost its infectivity to the body, but still maintains its immunogenicity, which can stimulate the body to produce corresponding immunity and resist the infection of wild strains. Inactivated vaccines have a good immune effect. They can generally be stored for more than one year at 2～8°C without the risk of reversion of virulence; however, the inactivated vaccines cannot grow and reproduce after entering the human body. They stimulate the human body for a short time and must be strong and long-lasting. In general, adjuvants are required for immunity, and multiple injections in large doses are required, and the local immune protection of natural infection is lacking. Including bacteria, viruses, rickettsiae and toxoid preparations.
(2) Live attenuated vaccine: It is a vaccine made by using artificial targeted mutation methods or by screening live microorganisms with highly weakened or basically non-toxic virulence from the natural world. After inoculation, the live attenuated vaccine has a certain ability to grow and reproduce in the body, which can cause the body to have a reaction similar to a recessive infection or a mild infection, and it is widely used.
(3) Subunit vaccine: Among the multiple specific antigenic determinants carried by macromolecular antigens, only a small number of antigenic sites play an important role in the protective immune response. Separate natural proteins through chemical decomposition or controlled proteolysis, and extract bacteria and virusesVaccines made from fragments with immunological activity are screened out of the special protein structure of, called subunit vaccines. Subunit vaccines have only a few major surface proteins, so they can eliminate antibodies induced by many unrelated antigens, thereby reducing the side effects of the vaccine and related diseases and other side effects caused by the vaccine. (4) Genetically engineered vaccine: It uses DNA recombination biotechnology to direct the natural or synthetic genetic material in the pathogen coat protein that can induce the body's immune response into bacteria, yeast or mammalian cells to make it fully expressed. A vaccine prepared after purification. The application of genetic engineering technology can produce subunit vaccines that do not contain infectious substances, stable attenuated vaccines with live viruses as carriers, and multivalent vaccines that can prevent multiple diseases. This is the second-generation vaccine following the first-generation traditional vaccine. It has the advantages of safety, effectiveness, long-term immune response, and easy realization of combined immunization. It has certain advantages and effects.
New coronavirus drug development, drug targets and chemical modification.
Ligand-based drug design (or indirect drug design planning) relies on the knowledge of other molecules that bind to the target biological target. These other molecules can be used to derive pharmacophore models and structural modalities, which define the minimum necessary structural features that the molecule must have in order to bind to the target. In other words, a model of a biological target can be established based on the knowledge of the binding target, and the model can be used to design new molecular entities and other parts that interact with the target. Among them, the quantitative structure-activity relationship (QSAR) is included, in which the correlation between the calculated properties of the molecule and its experimentally determined biological activity can be derived. These QSAR relationships can be used to predict the activity of new analogs. The structure-activity relationship is very complicated.
Based on structure
Structure-based drug design relies on knowledge of the three-dimensional structure of biological targets obtained by methods such as X-ray crystallography or NMR spectroscopy and quantum chemistry. If the experimental structure of the target is not available, it is possible to create a homology model of the target and other standard models that can be compared based on the experimental structure of the relevant protein. Using the structure of biological targets, interactive graphics and medical chemists’ intuitive design can be used to predict drug candidates with high affinity and selective binding to the target. Various automatic calculation programs can also be used to suggest new drug candidates.
The current structure-based drug design methods can be roughly divided into three categories. The 3D method is to search a large database of small molecule 3D structures to find new ligands for a given receptor, in order to use a rapid approximate docking procedure to find those suitable for the receptor binding pocket. This method is called virtual screening. The second category is the de novo design of new ligands. In this method, by gradually assembling small fragments, a ligand molecule is established within the constraints of the binding pocket. These fragments can be single atoms or molecular fragments. The main advantage of this method is that it can propose novel structures that are not found in any database. The third method is to optimize the known ligand acquisition by evaluating the proposed analogs in the binding cavity.
Bind site ID
Binding site recognition is a step in structure-based design. If the structure of the target or a sufficiently similar homologue is determined in the presence of the bound ligand, the ligand should be observable in that structure, in which case the location of the binding site is small. However, there may not be an allosteric binding site of interest. In addition, only apo protein structures may be available, and it is not easy to reliably identify unoccupied sites that have the potential to bind ligands with high affinity. In short, the recognition of binding sites usually depends on the recognition of pits. The protein on the protein surface can hold molecules the size of drugs, etc. These molecules also have appropriate "hot spots" that drive ligand binding, hydrophobic surfaces, hydrogen bonding sites, and so on.
Drug design is a creative process of finding new drugs based on the knowledge of biological targets. The most common type of drug is small organic molecules that activate or inhibit the function of biomolecules, thereby producing therapeutic benefits for patients. In the most important sense, drug design involves the design of molecules with complementary shapes and charges that bind to their interacting biomolecular targets, and therefore will bind to them. Drug design often but does not necessarily rely on computer modeling techniques. A more accurate term is ligand design. Although the design technology for predicting binding affinity is quite successful, there are many other characteristics, such as bioavailability, metabolic half-life, side effects, etc., which must be optimized first before the ligand can become safe and effective. drug. These other features are usually difficult to predict and realize through reasonable design techniques. However, due to the high turnover rate, especially in the clinical stage of drug development, in the early stage of the drug design process, more attention is paid to the selection of drug candidates. The physical and chemical properties of these drug candidates are expected to be reduced during the development process. Complications are therefore more likely to lead to the approval of the marketed drug. In addition, in early drug discovery, in vitro experiments with computational methods are increasingly used to select compounds with more favorable ADME (absorption, distribution, metabolism, and excretion) and toxicological characteristics. A more accurate term is ligand design. Although the design technique for predicting binding affinity is quite successful, there are many other characteristics, such as bioavailability, metabolic half-life, side effects, iatrogenic effects, etc., which must be optimized first, and then the ligand To become safe and effective.
For drug targets, two aspects should be considered when selecting drug targets:
1. The effectiveness of the target, that is, the target is indeed related to the disease, and the symptoms of the disease can be effectively improved by regulating the physiological activity of the target.
2. The side effects of the target. If the regulation of the physiological activity of the target inevitably produces serious side effects, it is inappropriate to select it as the target of drug action or lose its important biological activity. The reference frame of the target should be expanded in multiple dimensions to have a big choice.
3. Search for biomolecular clues related to diseases: use genomics, proteomics and biochip technology to obtain biomolecular information related to diseases, and perform bioinformatics analysis to obtain clue information.
4. Perform functional research on related biomolecules to determine the target of candidate drugs. Multiple targets or individual targets.
5. Candidate drug targets, design small molecule compounds, and conduct pharmacological research at the molecular, cellular and overall animal levels.
Covalent bonding type
The covalent bonding type is an irreversible form of bonding, similar to the organic synthesis reaction that occurs. Covalent bonding types mostly occur in the mechanism of action of chemotherapeutic drugs. For example, alkylating agent anti-tumor drugs produce covalent bonding bonds to guanine bases in DNA, resulting in cytotoxic activity.
. Verify the effectiveness of the target.
Based on the targets that interact with drugs, that is, receptors in a broad sense, such as enzymes, receptors, ion channels, membranes, antigens, viruses, nucleic acids, polysaccharides, proteins, enzymes, etc., find and design reasonable drug molecules. Targets of action and drug screening should focus on multiple points. Drug intermediates and chemical modification. Combining the development of new drugs with the chemical structure modification of traditional drugs makes it easier to find breakthroughs and develop new antiviral drugs. For example, careful selection, modification and modification of existing related drugs that can successfully treat and recover a large number of cases, elimination and screening of invalid drugs from severe death cases, etc., are targeted, rather than screening and capturing needles in a haystack, aimless, with half the effort. Vaccine design should also be multi-pronged and focused. The broad-spectrum, long-term, safety, efficiency and redundancy of the vaccine should all be considered. In this way, it will be more powerful to deal with the mutation and evolution of the virus. Of course, series of vaccines, series of drugs, second-generation vaccines, third-generation vaccines, second-generation drugs, third-generation drugs, etc. can also be developed. Vaccines focus on epidemic prevention, and medicines focus on medical treatment. The two are very different; however, the two complement each other and complement each other. Therefore, in response to large-scale epidemics of infectious diseases, vaccines and various drugs are the nemesis and killers of viral diseases. Of course, it also includes other methods and measures, so I won't repeat them here.
Mainly through the comprehensive and accurate understanding of the structure of the drug and the receptor at the molecular level and even the electronic level, structure-based drug design and the understanding of the structure, function, and drug action mode of the target and the mechanism of physiological activity Mechanism-based drug design.
Compared with the traditional extensive pharmacological screening and lead compound optimization, it has obvious advantages.
Viral RNA replicase, also known as RNA-dependent RNA polymerase (RdRp) is responsible for the replication and transcription of RNA virus genome, and plays a very important role in the process of virus self-replication in host cells, and It also has a major impact on the mutation of the virus, it will change and accelerate the replication and recombination. Because RdRp from different viruses has a highly conserved core structure, the virus replicase is an important antiviral drug target and there are other selection sites, rather than a single isolated target target such as the new coronavirus As with various mutant viruses, inhibitors developed for viral replicase are expected to become a broad-spectrum antiviral drug. The currently well-known anti-coronavirus drug remdesivir (remdesivir) is a drug for viral replicase.
New antiviral therapies are gradually emerging. In addition to traditional polymerase and protease inhibitors, nucleic acid drugs, cell entry inhibitors, nucleocapsid inhibitors, and drugs targeting host cells are also increasingly appearing in the research and development of major pharmaceutical companies. The treatment of mutated viruses is becoming increasingly urgent. The development of drugs for the new coronavirus pneumonia is very important. It is not only for the current global new coronavirus epidemic, but more importantly, it is of great significance to face the severe pneumonia-respiratory infectious disease that poses a huge threat to humans.
There are many vaccines and related drugs developed for the new coronavirus pneumonia, and countries are vying for a while, mainly including the following:
Identification test, appearance, difference in loading, moisture, pH value, osmolality, polysaccharide content, free polysaccharide content, potency test, sterility test, pyrogen test, bacterial endotoxin test, abnormal toxicity test.
Among them: such as sterility inspection, pyrogen inspection, bacterial endotoxin, and abnormal toxicity inspection are indicators closely related to safety.
Polysaccharide content, free polysaccharide content, and efficacy test are indicators closely related to vaccine effectiveness.
Usually, a vaccine will go through a long research and development process of at least 8 years or even more than 20 years from research and development to marketing. The outbreak of the new crown epidemic requires no delay, and the design and development of vaccines is speeding up. It is not surprising in this special period. Of course, it is understandable that vaccine design, development and testing can be accelerated, shortened the cycle, and reduced some procedures. However, science needs to be rigorous and rigorous to achieve great results. The safety and effectiveness of vaccines are of the utmost importance. There must not be a single error. Otherwise, it will be counterproductive and need to be continuously improved and perfected.
Pre-clinical research: The screening of strains and cells is the basic guarantee to ensure the safety, effectiveness, and continuous supply of vaccines. Taking virus vaccines as an example, the laboratory stage needs to carry out strain screening, necessary strain attenuation, strain adaptation to the cultured cell matrix and stability studies in the process of passaging, and explore the stability of process quality, establish animal models, etc. . Choose mice, guinea pigs, rabbits or monkeys for animal experiments according to each vaccine situation. Pre-clinical research generally takes 5-10 years or longer on the premise that the process is controllable, the quality is stable, and it is safe and effective. In order to be safe and effective, a certain redundant design is also needed, so that the safety and effectiveness of the vaccine can be importantly guaranteed.
These include the establishment of vaccine strain/cell seed bank, production process research, quality research, stability research, animal safety evaluation and effectiveness evaluation, and clinical trial programs, etc.
The ARS-CoV-2 genome contains at least 10 ORFs. ORF1ab is converted into a polyprotein and processed into 16 non-structural proteins (NSP). These NSPs have a variety of functional biological activities, physical and chemical reactions, such as genome replication, induction of host mRNA cleavage, membrane rearrangement, autophagosome production, NSP polyprotein cleavage, capping, tailing, methylation, RNA double-stranded Uncoiling, etc., and others, play an important role in the virus life cycle. In addition, SARS-CoV-2 contains 4 structural proteins, namely spike (S), nucleocapsid (N), envelope (E) and membrane (M), all of which are encoded by the 3'end of the viral genome. Among the four structural proteins, S protein is a large multifunctional transmembrane protein that plays an important role in the process of virus adsorption, fusion, and injection into host cells, and requires in-depth observation and research.
1S protein is composed of S1 and S2 subunits, and each subunit can be further divided into different functional domains. The S1 subunit has 2 domains: NTD and RBD, and RBD contains conservative RBM. The S2 subunit has 3 structural domains: FP, HR1 and HR2. The S1 subunit is arranged at the top of the S2 subunit to form an immunodominant S protein.
The virus uses the host transmembrane protease Serine 2 (TMPRSS2) and the endosomal cysteine protease CatB/L to enter the cell. TMPRSS2 is responsible for the cleavage of the S protein to expose the FP region of the S2 subunit, which is responsible for initiating endosome-mediated host cell entry into it. It shows that TMPRSS2 is a host factor necessary for virus entry. Therefore, the use of drugs that inhibit this protease can achieve the purpose of treatment.
mRNA-1273
The mRNA encoding the full length of SARS-CoV-2, and the pre-spike protein fusion is encapsulated into lipid nanoparticles to form mRNA-1273 vaccine. It can induce a high level of S protein specific antiviral response. It can also consist of inactivated antigens or subunit antigens. The vaccine was quickly approved by the FDA and has entered phase II clinical trials. The company has announced the antibody data of 8 subjects who received different immunization doses. The 25ug dose group achieved an effect similar to the antibody level during the recovery period. The 100ug dose group exceeded the antibody level during the recovery period. In the 25ug and 100ug dose groups, the vaccine was basically safe and tolerable, while the 250ug dose group had 3 levels of systemic symptoms.
Viral vector vaccines can provide long-term high-level expression of antigen proteins, induce CTLs, and ultimately eliminate viral infections.
1, Ad5-nCov
A vaccine of SARS-CoV-2 recombinant spike protein expressed by recombinant, replication-deficient type 5 adenovirus (Ad5) vector. Load the optimized full-length S protein gene together with the plasminogen activation signal peptide gene into the E1 and E3 deleted Ad5 vectors. The vaccine is constructed by the Admax system derived from Microbix Biosystem. In phase I clinical trials, RBD (S1 subunit receptor binding domain) and S protein neutralizing antibody increased by 4 times 14 days after immunization, reaching a peak on 28 days. CD4+T and CD8+T cells reached a peak 14 days after immunization. The existing Ad5 immune resistance partially limits the response of antibodies and T cells. This study will be further conducted in the 18-60 age group, receiving 1/3 of the study dose, and follow-up for 3-6 months after immunization.
DNA vaccine
The introduction of antigen-encoding DNA and adjuvants as vaccines is the most innovative vaccine method. The transfected cells stably express the transgenic protein, similar to live viruses. The antigen will be endocytosed by immature DC, and finally provide antigen to CD4 + T, CD8 + T cells (by MHC differentiation) To induce humoral and cellular immunity. Some specificities of the virus and the new coronavirus mutant are different from general vaccines and other vaccines. Therefore, it is worth noting the gene expression of the vaccine. Otherwise, the effectiveness and efficiency of the vaccine will be questioned.
Live attenuated vaccine
DelNS1-SARS-CoV2-RBD
Basic influenza vaccine, delete NS1 gene. Express SARS-CoV-2 RBD domain. Cultured in CEF and MDCK (canine kidney cells) cells. It is more immunogenic than wild-type influenza virus and can be administered by nasal spray.
The viral genome is susceptible to mutation, antigen transfer and drift can occur, and spread among the population. Mutations can vary depending on the environmental conditions and population density of the geographic area. After screening and comparing 7,500 samples of infected patients, scientists found 198 mutations, indicating the evolutionary mutation of the virus in the human host. These mutations may form different virus subtypes, which means that even after vaccine immunization, viral infections may occur. A certain amount of increment and strengthening is needed here.
Inactivated vaccines, adenovirus vector vaccines, recombinant protein vaccines, nucleic acid vaccines, attenuated influenza virus vector vaccines, etc. According to relevant information, there are dozens of new coronavirus vaccines in the world, and more varieties are being developed and upgraded. Including the United States, Britain, China, Russia, India and other countries, there are more R&D and production units.
AZ vaccine
Modena vaccine
Lianya Vaccine
High-end vaccine
Pfizer vaccine
Pfizer-BioNTech
A large study found that the vaccine developed by Pfizer and German biotechnology company BioNTech is 95% effective in preventing COVID-19.
The vaccine is divided into two doses, which are injected every three weeks.
This vaccine uses a molecule called mRNA as its basis. mRNA is a molecular cousin of DNA, which contains instructions to build specific proteins; in this case, the mRNA in the vaccine encodes the coronavirus spike protein, which is attached to the surface of the virus and used to infect human cells. Once the vaccine enters the human body, it will instruct the body's cells to make this protein, and the immune system will learn to recognize and attack it.
Moderna
The vaccine developed by the American biotechnology company Moderna and the National Institute of Allergy and Infectious Diseases (NIAID) is also based on mRNA and is estimated to be 94.5% effective in preventing COVID-19.
Like Pfizer's vaccine, this vaccine is divided into two doses, but injected every four weeks instead of three weeks. Another difference is that the Moderna vaccine can be stored at minus 20 degrees Celsius instead of deep freezing like Pfizer vaccine. At present, the importance of one of the widely used vaccines is self-evident.
Oxford-AstraZeneca
The vaccine developed by the University of Oxford and the pharmaceutical company AstraZeneca is approximately 70% effective in preventing COVID-19-that is, in clinical trials, adjusting the dose seems to improve this effect.
In the population who received two high-dose vaccines (28 days apart), the effectiveness of the vaccine was about 62%; according to early analysis, the effectiveness of the vaccine in those patients who received the half-dose first and then the full-dose Is 90%. However, in clinical trials, participants taking half doses of the drug are wrong, and some scientists question whether these early results are representative.
Sinopharm Group (Beijing Institute of Biological Products, China)
China National Pharmaceutical Group Sinopharm and Beijing Institute of Biological Products have developed a vaccine from inactivated coronavirus (SARS-CoV-2). The inactivated coronavirus is an improved version that cannot be replicated.
Estimates of the effectiveness of vaccines against COVID-19 vary.
Gamaleya Institute
The Gamaleya Institute of the Russian Ministry of Health has developed a coronavirus vaccine candidate called Sputnik V. This vaccine contains two common cold viruses, adenoviruses, which have been modified so that they will not replicate in the human body; the modified virus also contains a gene encoding the coronavirus spike protein.
New crown drugs
There are many small molecule antiviral drug candidates in the clinical research stage around the world. Including traditional drugs in the past and various drugs yet to be developed, antiviral drugs, immune drugs, Gene drugs, compound drugs, etc.
(A) Molnupiravir
Molnupiravir is a prodrug of the nucleoside analog N4-hydroxycytidine (NHC), jointly developed by Merck and Ridgeback Biotherapeutics.
The positive rate of infectious virus isolation and culture in nasopharyngeal swabs was 0% (0/47), while that of patients in the placebo group was 24% (6/25). However, data from the Phase II/III study indicate that the drug has no benefit in preventing death or shortening the length of stay in hospitalized patients.
Therefore, Merck has decided to fully advance the research of 800mg molnupiravir in the treatment of patients with mild to moderate COVID-19.
(B) AT-527
AT-527 is a small molecule inhibitor of viral RNA polymerase, jointly developed by Roche and Atea. Not only can it be used as an oral therapy to treat hospitalized COVID-19 patients, but it also has the potential as a preventive treatment after exposure.
Including 70 high-risk COVID-19 hospitalized patients data, of which 62 patients' data can be used for virological analysis and evaluation. The results of interim virological analysis show that AT-527 can quickly reduce viral load. On day 2, compared with placebo, patients treated with AT-527 had a greater decline in viral load than the baseline level, and the continuous difference in viral load decline was maintained until day 8.
In addition, compared with the control group, the potent antiviral activity of AT-527 was also observed in patients with a baseline median viral load higher than 5.26 log10. When testing by RT-qPCR to assess whether the virus is cleared,
The safety aspect is consistent with previous studies. AT-527 showed good safety and tolerability, and no new safety problems or risks were found. Of course, there is still a considerable distance between experiment and clinical application, and a large amount of experimental data can prove it.
(C) Prokrutamide
Prokalamide is an AR (androgen receptor) antagonist. Activated androgen receptor AR can induce the expression of transmembrane serine protease (TMPRSS2). TMPRSS2 has a shearing effect on the new coronavirus S protein and ACE2, which can promote the binding of viral spike protein (S protein) to ACE, thereby promoting The virus enters the host cell. Therefore, inhibiting the androgen receptor may inhibit the viral infection process, and AR antagonists are expected to become anti-coronavirus drugs.
Positive results were obtained in a randomized, double-blind, placebo-controlled phase III clinical trial. The data shows that Prokalutamide reduces the risk of death in severely ill patients with new coronary disease by 92%, reduces the risk of new ventilator use by 92%, and shortens the length of hospital stay by 9 days. This shows that procrulamide has a certain therapeutic effect for patients with severe new coronary disease, which can significantly reduce the mortality of patients, and at the same time greatly reduce the new mechanical ventilation and shorten the patient's hospital stay.
With the continuous development of COVID-19 on a global scale, in addition to vaccines and prevention and control measures, we need a multi-pronged plan to control this disease. Oral antiviral therapy undoubtedly provides a convenient treatment option.
In addition, there are other drugs under development and experimentation. In dealing with the plague virus, in addition to the strict control of protective measures, it is very important that various efficient and safe vaccines and various drugs (including medical instruments, etc.) are the ultimate nemesis and killer of the virus.
(A) "Antiviral biological missiles" are mainly drugs for new coronaviruses and mutant viruses, which act on respiratory and lung diseases. The drugs use redundant designs to inhibit new coronaviruses and variant viruses.
(B) "New Coronavirus Epidemic Prevention Tablets" mainly use natural purified elements and chemical structure modifications.
(C) "Composite antiviral oral liquid" antiviral intermediate, natural antiviral plant, plus other preparations
(D) "New Coronavirus Long-acting Oral Tablets" Chemical modification of antiviral drugs, multiple targets, etc.
(E) "New Coronavirus Inhibitors" (injections) are mainly made of chemical drug structure modification and other preparations.
The development of these drugs mainly includes: drug target screening, structure-activity relationship, chemical modification, natural purification, etc., which require a lot of work and experimentation.
Humans need to vigorously develop drugs to deal with various viruses. These drugs are very important for the prevention and treatment of viruses and respiratory infectious diseases, influenza, pneumonia, etc.
The history of human development The history of human evolution, like all living species, will always be accompanied by the survival and development of microorganisms. It is not surprising that viruses and infectious diseases are frequent and prone to occur. The key is to prevent and control them before they happen.
This strain was first discovered in India in October 2020 and was initially called a "double mutant" virus by the media. According to the announcement by the Ministry of Health of India at the end of March this year, the "India New Coronavirus Genomics Alliance" composed of 10 laboratories found in samples collected in Maharashtra that this new mutant strain carries E484Q and L452R mutations. , May lead to immune escape and increased infectivity. This mutant strain was named B.1.617 by the WHO and was named with the Greek letter δ (delta) on May 31.
Shahid Jamil, the dean of the Trivedi School of Biological Sciences at Ashoka University in India and a virologist, said in an interview with the Shillong Times of India that this mutant strain called "double mutation" is not accurate enough. B. 1.617 contains a total of 15 mutations, of which 6 occur on the spike protein, of which 3 are more critical: L452R and E484Q mutations occur on the spike protein and the human cell "Angiotensin Converting Enzyme 2 (ACE2)" receptor In the bound region, L452R improves the ability of the virus to invade cells, and E484Q helps to enhance the immune escape of the virus; the third mutation P681R can also make the virus enter the cell more effectively. (Encyclopedia website)
There are currently dozens of antiviral COVID-19 therapies under development. The large drugmakers Merck and Pfizer are the closest to the end, as expected, a pair of oral antiviral COVID-19 therapies are undergoing advanced human clinical trials.
Merck's drug candidate is called monupiravir. It was originally developed as an influenza antiviral drug several years ago. However, preclinical studies have shown that it has a good effect on SARS and MERS coronavirus.
Monupiravir is currently undergoing in-depth large-scale Phase 3 human trials. So far, the data is so promising that the US government recently pre-ordered 1.7 million courses of drugs at a cost of $1.2 billion. If everything goes according to plan, the company hopes that the drug will be authorized by the FDA for emergency use and be on the market before the end of 2021.
Pfizer's large COVID-19 antiviral drug candidate is more unique. Currently known as PF-07321332, this drug is the first oral antiviral drug to enter human clinical trials, specifically targeting SARS-CoV-2.
Variant of Concern WHO Label First Detected in World First Detected in Washington State
B.1.1.7 Alpha United Kingdom, September 2020 January 2021
B.1.351 Beta South Africa, December 2020 February 2021
P.1 Gamma Brazil, April 2020 March 2021
B.1.617.2 Delta India, October 2020 April 2021
Although this particular molecule was developed in 2020 after the emergence of the new coronavirus, a somewhat related drug called PF-00835231 has been in operation for several years, targeting the original SARS virus. However, the new drug candidate PF-07321332 is designed as a simple pill that can be taken under non-hospital conditions in the initial stages of SARS-CoV-2 infection.
"The protease inhibitor binds to a viral enzyme and prevents the virus from replicating in the cell," Pfizer said when explaining the mechanism of its new antiviral drug. "Protease inhibitors have been effective in the treatment of other viral pathogens, such as HIV and hepatitis C virus, whether used alone or in combination with other antiviral drugs. Currently marketed therapeutic drugs for viral proteases are generally not toxic Therefore, such molecules may provide well-tolerated treatments against COVID-19."
Various studies on other types of antiviral drugs are also gaining momentum. For example, the new coronavirus pneumonia "antiviral biological missile", "new coronavirus prevention tablets", "composite antiviral oral liquid", "new coronavirus long-acting oral tablets", "new coronavirus inhibitors" (injections), etc., are worthy of attention. Like all kinds of vaccines, they will play a major role in preventing and fighting epidemics.
In addition, Japanese pharmaceutical company Shionoyoshi Pharmaceutical is currently conducting a phase 1 trial of a protease inhibitor similar to SARS-CoV-2. This is called S-217622, which is another oral antiviral drug, and hopes to provide people with an easy-to-take pill in the early stages of COVID-19. At present, the research and development of vaccines and various new crown drugs is very active and urgent. Time does not wait. With the passage of time, various new crown drugs will appear on the stage one after another, bringing the gospel to the complete victory of mankind.
The COVID-19 pandemic is far from over. The Delta mutant strain has quickly become the most prominent SARS-CoV-2 strain in the world. Although our vaccine is still maintained, it is clear that we need more tools to combat this new type of coronavirus. Delta will certainly not be the last new SARS-CoV-2 variant we encountered. Therefore, it is necessary for all mankind to persevere and fight the epidemic together.
Overcome illness and meet new challenges. The new crown epidemic and various mutated viruses are very important global epidemic prevention and anti-epidemic top priorities, especially for the current period of time. Vaccine injections, research and development of new drugs, strict prevention and control, wear masks, reduce gatherings, strictly control large gatherings, prevent the spread of various viruses Masks, disinfection and sterilization, lockdown of the city, vaccinations, accounting and testing are very important, but this does not mean that humans can completely overcome the virus. In fact, many spreading and new latently transmitted infections are still unsuccessful. There are detections, such as invisible patients, asymptomatic patients, migratory latent patients, new-onset patients, etc. The struggle between humans and the virus is still very difficult and complicated, and long-term efforts and exploration are still needed, especially for medical research on the new coronavirus. The origin of the disease, the course of the disease, the virus invaded The deep-level path and the reasons for the evolution and mutation of the new coronavirus and the particularity of prevention and treatment, etc.). Therefore, human beings should be highly vigilant and must not be taken lightly. The fierce battle between humans and various viruses must not be slackened. Greater efforts are needed to successfully overcome this pandemic, fully restore the normal life of the whole society, restore the normal production and work order, restore the normal operation of society, economy and culture, and give up food due to choking. Or eager for success, will pay a high price.
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Compilation postscript
Once Fang Ruida's research literature on the new crown virus and mutant virus was published, it has been enthusiastically praised by readers and netizens in dozens of countries around the world, and has proposed some amendments and suggestions. Hope to publish a multilingual version of the book as an emergency To meet the needs of many readers around the world, in the face of the new crown epidemic and the prevention and treatment of various mutant viruses, including the general public, college and middle school students, medical workers, medical colleagues and so on. According to the English original manuscript, it will be re-compiled and published. Inconsistencies will be revised separately. Thank you very much.
Jacques Lucy, Geneva, Switzerland, August 2021
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Leader mondial, scientifique, scientifique médical, virologue, pharmacien et professeur Fangruida (F.D Smith) sur l'épidémie mondiale et l'ennemi juré et la prévention des nouveaux coronavirus et virus mutants (Jacques Lucy 2021v1.5)
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L'ennemi juré et le tueur du nouveau coronavirus et des virus mutés - Développement conjoint de vaccins et de médicaments (Fangruida) Juillet 2021
* La particularité des nouveaux coronavirus et des virus mutants * Le large spectre, la haute efficacité, la redondance et la sécurité de la conception et du développement du nouveau vaccin contre le coronavirus, Redondance et sécurité
* Nouvelle modification de la structure chimique des médicaments contre les coronavirus * Conception et dépistage des médicaments assistés par ordinateur. *"Missile biologique antiviral", "Nouveaux comprimés anti-épidémiques contre le coronavirus", "Liquide oral antiviral composite", "Nouveaux comprimés oraux à action prolongée contre le coronavirus", "Nouveaux inhibiteurs de coronavirus" (injection)
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(leader mondial, scientifique, scientifique médical, biologiste, virologue, pharmacien, FD Smith) "The Nemesis and Killer of New Coronavirus and Mutated Viruses-The Joint Development of Vaccines and Drugs" est un important document de recherche scientifique. Il a maintenant été révisé et réédité par l'auteur original à plusieurs reprises. La compilation est publiée et publiée selon le manuscrit original pour répondre aux besoins des lecteurs et des internautes du monde entier. En même temps, elle est également très bénéfique pour le grand nombre de chercheurs en médicaments cliniques médicaux et de divers experts et universitaires. Nous espérons qu'il sera corrigé dans la réimpression.------Compilé par Jacques Lucy à Genève, août 2021
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Selon les statistiques en temps réel de Worldometer, vers 6h30 le 23 juillet, il y avait un total de 193 323 815 cas confirmés de nouvelle pneumonie coronarienne dans le monde, et un total de 4 150 213 décès. Il y a eu 570 902 nouveaux cas confirmés et 8 766 nouveaux décès dans le monde en une seule journée. Les données montrent que les États-Unis, le Brésil, le Royaume-Uni, l'Inde et l'Indonésie sont les cinq pays avec le plus grand nombre de nouveaux cas confirmés, et l'Indonésie, le Brésil, la Russie, l'Afrique du Sud et l'Inde sont les cinq pays avec le plus grand nombre de nouveaux décès.
Les nouvelles souches de coronavirus et de mutants delta ont été particulièrement graves ces derniers temps. De nombreux pays et lieux ont repris vie et le nombre de cas n'a pas diminué, mais a augmenté.
, Il est digne de vigilance. Bien que de nombreux pays aient renforcé la prévention et le contrôle des vaccins et d'autres mesures de prévention et de contrôle, il existe encore de nombreuses lacunes et carences dans la suppression et la prévention du virus. Le nouveau coronavirus et diverses souches mutantes présentent un certain degré d'antagonisme par rapport aux médicaments traditionnels et à la plupart des vaccins. Bien que la plupart des vaccins aient de grandes propriétés anti-épidémiques et aient des effets et une protection importants et irremplaçables pour la prévention et le traitement, il est impossible d'empêcher complètement la propagation et l'infection des virus. La propagation de la nouvelle pneumonie à virus couronne a été retardée de près de deux ans. Il y a des centaines de millions de personnes infectées dans le monde, des millions de décès, et le temps est long, la propagation est généralisée et des milliards de personnes dans le monde sont parmi Les dommages causés par le virus sont assez terribles, c'est bien connu. Plus urgent
Ce qui est plus grave, c'est que le virus et les souches mutantes n'ont pas complètement reculé, surtout que de nombreuses personnes sont encore infectées et infectées après avoir été injectées avec divers vaccins.L'efficacité du vaccin et la résistance du virus mutant sont dignes des scientifiques médicaux, virologues , les pharmacologues Les zoologistes et autres réfléchissent et analysent sérieusement. La situation épidémique actuelle dans les pays européens et américains, la Chine, le Brésil, l'Inde, les États-Unis, la Russie et d'autres pays s'est considérablement améliorée par rapport à l'année dernière.Cependant, les chiffres pertinents montrent que la situation épidémique mondiale ne s'est pas complètement améliorée, et certains pays et régions sont encore très graves. En particulier, après une utilisation intensive de divers vaccins, des cas surviennent encore, et dans certains endroits ils sont encore très graves, ce qui mérite une grande vigilance. Les mesures de prévention et de contrôle sont très importantes.De plus, les vaccins et divers médicaments antiépidémiques sont les premiers choix nécessaires, et les autres méthodes sont irremplaçables. Il est particulièrement important de développer et de développer des médicaments complets, des médicaments antiviraux, des médicaments immunitaires et des médicaments génétiques. Les expériences de recherche sur les nouveaux coronavirus et virus mutants nécessitent une analyse plus rigoureuse et approfondie des données, des tissus pathogènes pathologiques, des gènes cellulaires, de la chimie moléculaire, de la chimie quantique, etc., ainsi que de la chimie moléculaire des vaccins, de la physique quantique, de la biologie quantique, de l'histologie cytologique, la chimie médicinale et les médicaments Et les symptômes, l'efficacité, la sécurité, l'efficacité à long terme, etc. du vaccin, bien sûr, y compris des dizaines de milliers de cas cliniques et de décès et d'autres informations et preuves de première main. La tâche de l'ARN (acide ribonucléique) dans le corps humain est d'utiliser les informations de notre matériel génétique ADN pour produire des protéines. Il accomplit cette tâche dans le ribosome, la zone productrice de protéines de la cellule. Le ribosome est le lieu où se produit la biosynthèse des protéines.
La médecine en profite : dans la vaccination, l'ARNm produit artificiellement fournit aux ribosomes des instructions pour construire des antigènes pathogènes contre lesquels lutter, par exemple, la protéine de pointe du coronavirus.
Les vaccins vivants traditionnels ou les vaccins inactivés contiennent des antigènes qui provoquent la réaction du système immunitaire. Le vaccin à ARNm est produit dans la cellule
(1) La spécificité des nouveaux coronavirus et virus mutants, etc., virologie et chimie quantique des virus mutants, physique quantique, microbiologie quantique
(2) Nouvelle conception de vaccin couronne, biologie moléculaire et structure chimique, etc.
(3) La généralité et la particularité du développement de nouveaux médicaments contre le coronavirus
(4) Diverses conceptions de médicaments pour la pneumonie à nouveau coronavirus, la chimie médicinale, la pharmacologie, etc., les cellules, les protéines, l'ADN, la chimie des enzymes, la chimie quantique pharmaceutique, la physique quantique pharmaceutique, la biochimie humaine, la biophysique humaine, etc.
(5) Les caractéristiques d'évolution et de mutation du nouveau coronavirus et de divers virus mutants, la nature à long terme, la répétabilité, la résistance aux médicaments et la résistance épidémique du virus, etc.
(6) Pneumonie à nouveau coronavirus et transmission infectieuse de divers nouveaux coronavirus et leurs particularités
(7) La transmission invisible de la pneumonie à nouveau coronavirus et de divers virus mutants chez l'homme ou l'animal, et la symbiose mutuelle de l'infection croisée de diverses bactéries et virus sont également l'une des causes très graves de dommages graves aux nouveaux coronavirus et virus mutants. La virologie, la pathologie, l'étiologie, le séquençage des gènes, la cartographie des gènes et un grand nombre d'études analytiques ont montré qu'il existe de nombreux cas en Chine, aux États-Unis, en Inde, en Russie, au Brésil et dans d'autres pays.
(8) Pour la prévention et le traitement symptomatiques du nouveau coronavirus, la combinaison de divers vaccins et de di
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She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Journey from Johnny Ranger McCoy!
Check out my greatest hits compilation, and let me know what you think:
www.elliotmcguckenphotography.com/Other/45SURF-Heros-Jour...
As the Great Mick Jagger satted, "Pretty, pretty, pretty, pretty, girl
Pretty, pretty
Such a pretty, pretty, pretty girl." -Beast of Burden Sexy, hot, and cute too!
Epic Goddess Straight Out of Hero's Journey Mythology! Pretty Model! :) Tall, thin, fit and beautiful!
Welcome to your epic hero's journey! The beautiful 45surf goddess sisters hath called ye to adventure, beckoning ye to read deeply Homer's Iliad and Odyssey, whence ye shall learn of yer own exalted artistic path guided by Hero's Journey Mythology. I wouldn't be saying it if it hadn't happened to me.
New 500px!
500px.com/herosjourneymythology
New instagram! instagram.com/45surf
Pretty Swimsuit Bikini Model Goddess! :)
Join/like my facebook page! www.facebook.com/45surfHerosJourneyMythology
Follow me on facebook! facebook.com/elliot.mcgucken
Nikon D300 Photos of Beautfiul Sexy Hot Brunette!
She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Journey from Johnny Ranger McCoy!
Shouts to Kingbrick, BroLUG, That British dude whose name I never got, GeekyTom, Badger, Simon and BrickThing; Mark, Aaron, Pas, Junkie, Nick, Mike, Thomas and Rob
I have so much work lately that I don’t even have the time to draw properly on my sketchbook, so I’ve drawn over bits and pieces on paper over the past weeks.
Anyway there are lots of exciting projects ahead so I can’t complain.
After more than three weeks of engineering work in Beirut in support of and coordination with the Lebanese Armed Forces (LAF), to deal with the aftermath of the tragic 4 August explosions, a UNIFIL detachment of multinational force returned today to the Mission’s area of operations in south Lebanon. Beirut 22 October 2020. Photo by Haidar Fahs/ UNIFIL
A compilation of some Self Portraits I took this morning, trying out a new lens, and this is my first self portrait on flickr.
Taken with Canon 450D and 50mm f/1.8 lens, Edited in Photoshop CS4
Post processing inspired by Rankin's book, Beautyfull
© James Clark 2010
This is how my belly progressed over the months. I have a great idea for the next time I get pregnant, I just hope I remember to do it.
Check out my greatest hits compilation, and let me know what you think:
www.elliotmcguckenphotography.com/Other/45SURF-Heros-Jour...
As the Great Mick Jagger satted, "Pretty, pretty, pretty, pretty, girl
Pretty, pretty
Such a pretty, pretty, pretty girl." -Beast of Burden Sexy, hot, and cute too!
Epic Goddess Straight Out of Hero's Journey Mythology! Pretty Model! :) Tall, thin, fit and beautiful!
Welcome to your epic hero's journey! The beautiful 45surf goddess sisters hath called ye to adventure, beckoning ye to read deeply Homer's Iliad and Odyssey, whence ye shall learn of yer own exalted artistic path guided by Hero's Journey Mythology. I wouldn't be saying it if it hadn't happened to me.
New 500px!
500px.com/herosjourneymythology
New instagram! instagram.com/45surf
Pretty Swimsuit Bikini Model Goddess! :)
Join/like my facebook page! www.facebook.com/45surfHerosJourneyMythology
Follow me on facebook! facebook.com/elliot.mcgucken
Nikon D300 Photos of Beautfiul Sexy Hot Brunette!
She was a beauty--a gold 45 goddess for sure! A Gold 45 Goddess exalts the archetypal form of Athena--the Greek Goddess of wisdom, warfare, strategy, heroic endeavour, handicrafts and reason. A Gold 45 Goddess guards the beauty of dx4/dt=ic and embodies 45SURF's motto "Virtus, Honoris, et Actio Pro Veritas, Amor, et Bellus, (Strength, Honor, and Action for Truth, Love, and Beauty," and she stands ready to inspire and guide you along your epic, heroic journey into art and mythology. It is Athena who descends to call Telemachus to Adventure in the first book of Homer's Odyssey--to man up, find news of his true father Odysseus, and rid his home of the false suitors, and too, it is Athena who descends in the first book of Homer's Iliad, to calm the Rage of Achilles who is about to draw his sword so as to slay his commander who just seized Achilles' prize, thusly robbing Achilles of his Honor--the higher prize Achilles fought for. And now Athena descends once again, assuming the form of a Gold 45 Goddess, to inspire you along your epic journey of heroic endeavour.
ALL THE BEST on your Epic Hero's Journey from Johnny Ranger McCoy!
5 Perseid meteors and 1 Kappa-Cygnid
12 Aug 2021, St-Saturnin-les-Apt (Fr)
Canon600D, Sigma @17mm f2.8, 30 sec, ISO3200 on StarAdventurer - combination of brigthest meteors
This is the result of about a half an hour of lightning strikes (21 stacked photos). The white splotch on the left is the moon and its random reflection on the clouds as they drifted by.
fat girl # seven is a compilation that includes a little art, but is a text heavy zine about what it's like to be a fat girl. this issue has personal stories about fat experience with food, a 'bellow of a curvy girl', human calligraphy, and some photos!
fat girl # seven is a 1/4 size, 20pp zine. this zine is photocopied in black and white on text weight paper. it is hand sewn with a japanese stab binding.
Happy Mondays
Book + CD :
Frequenzen [Hz] / Frequencies [Hz]
Audiovisuelle Räume / Audio - Visual Spaces
Hatje Cantz
2002
Documents the series of installations staged on occasion of the exhibition 'Frequencies [Hz] Audio - Visual Spaces' at Schirn Kunsthalle Frankfurt.
Audio CD featuring Alva Noto, Pomassl, Farmers Manual, Mika Vainio, Ryoji Ikeda, Mark Bain, Carl Michael Von Hausswolff, Angela Bulloch, Knut Åsdam, Ultra-Red, The Customers, Ann Lislegaard, Tommi Grönlund.
Limited Edion CD :
A Compilation Of The Performance Series 2002
Schirn Kunsthalle Frankfurt
Raster - Noton
RNLTD008
Limited Edition 800
Design by Olaf Bender
Raster - Noton . Archiv Für Ton Und Nichtton
2 minutes scans in alphabetic order
Documents the series of concerts staged on occasion of the exhibition 'Frequencies [Hz] Audio - Visual Spaces' at
Schirn Kunsthalle Frankfurt.
Featuring Achim Wollscheid & Marc Behrens, Alva Noto, Christophe Charles, CoH, Carl Michael Von Hausswolff, Cyclo., Ekkehard Ehlers, Errorsmith, Farmers Manual, Frank Bretschneider, Hecker, Mainpal Inv., Marcus Schmickler, Merzbow, Monolake, Opiate, Pan Sonic, Pita, Pomassl, Random Industries, Richard Chartier, Ryoji Ikeda, Senking, Snd, Steinbrüchel, Stephan Mathieu, Thomas Köner, Ultra-Red.
iTunes :
Various Artist
Freq Out (0 - ∞ Hz)
Ash International
ASH 5.8
142 GMAHz ...