View allAll Photos Tagged transformations
Created by paper cutting artist Emmanuel José.
Blogged: www.allthingspaper.net/2012/12/cut-paper-playing-cards-em...
Genetics lab experiment - the students did a great job and it even worked beautifully! The goal was to make E. coli bacteria glow by making them take up and express a jellyfish gene for green fluorescent protein (GFP). The top two plates have bacteria that have taken up the gene (+pGLO), making them resistant to ampicillin (AMP) as the resistance gene is linked to the green fluorescent protein gene to allow for selection of positive clones. Without the gene (-pGLO) the bacteria cannot grow on the AMP plates. The GFP gene is hooked up to an arabinose-responsive promoter, so it will only be turned on if there is arabinose (ARA) present in the medium. And...
Juan Diego Castro, Director, National Partnerships, Mission Economic Development Agency (MEDA), USA; speaking in Building Community Ownership and Resilience: Mission District session at the Urban Transformation Summit 2025 in San Francisco, USA, 22/10/2025, 13:15 – 17:00, 118 - Presidio Campus. Shuttle 1. Copyright: World Economic Forum | Jason Steinberg
Day 2 Urban Transformation Summit 2022 in The Madison, Detroit, Mi on October 11
Shinola Factory Tour
Day 3 Urban Transformation Summit 2022 in The Madison, Detroit, Mi on October 12
Plenary Session: Enabling a Green and Just Recovery
Speakers: Abigail Campbell Singer, Senior Director; Head, Climate and Infrastructure Policy, Siemens Corporation; Anika Goss, CEO, Detroit Future City; George Benson, Manager, Economic Transformation, City of Vancouver; Michaeljohn Green, Deputy Director, Economic Development Miami-made County
There will be haters, there will be doubters, there will be non-believers and there will be YOU, proving them Wrong...
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She sure is growing up fast!
It is just crazy how quickly babies change, especially over the first couple of months. I am just so madly in love with her.
Our little Léa. xxx
19/11/14
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i took my western landscape history students on a field trip today through parts of sonoma county -- we stopped just outside of Sebastopol to check out the transformation of local agriculture from being dominated by apple orchards (right) to vineyards (left) -- we literally were standing in a driveway looking at these two fields on either side.
as much as i like wine, the take-over of these lands by grapevines is a little disturbing -- it's a much more intense kind of agriculture than orchards, and gives the area a completely different feeling -- we were standing on the Gravenstein Highway, after all, named for the local apple variety, but with fewer and fewer orchards each year...
if you click on Flickr's map feature for this image (and zoom in), you can really see the two fields distinctly from the aerial photo...
DOT Art partnered with the Garment District Alliance to present a sculptural installation by Hacer within pedestrian plazas along Broadway between 37th Street and 39th Street in Manhattan. Titled, “Transformations,” the colorful sculpture series featured seven unique origami inspired, life-size steel sculptures that created an interactive and playful experience for passersby of all ages.
NYC DOT Art Program, Arterventions (2021)
“Transformations” by Hacer
In partnership with the Garment District Alliance
Broadway between 37th Street and 39th Street, Manhattan
Tapping into our essential selves and what we care about on the first day of the Collective Healing Spaces gathering in New Smyrna Beach, Florida. August 2016
Day 3 Urban Transformation Summit 2022 in The Madison, Detroit, Mi on October 12
Plenary Session: Enabling a Green and Just Recovery
Speakers: Abigail Campbell Singer, Senior Director; Head, Climate and Infrastructure Policy, Siemens Corporation; Anika Goss, CEO, Detroit Future City; George Benson, Manager, Economic Transformation, City of Vancouver; Michaeljohn Green, Deputy Director, Economic Development Miami-made County
Marc Chun, a specialist in transformational education, will facilitate a two-day workshop designed for faculty to use their course content to expand critical thinking and problem solving in undergraduate courses
By W. Wayne Marlow
USAG Humphreys Public Affairs
CAMP HUMPHREYS — When Military Police Soldiers return from a deployment, it doesn’t necessarily mean they won’t be facing dangerous situations.
Incidents at Virginia Tech, Fort Hood, Texas, and Phoenix are vivid reminders of that.
However, the techniques and tactics that allowed for a successful deployment may not translate well in the United States.
“What we do in Iraq is not the same thing as in garrison,” said Cpl. Matthew Heydon, a team leader with the 557th Military Police Company.
With that in mind, the 557th trained on responding to active shooters on Feb. 24 and 25. The MPs conducted the paintball training in a former barracks building near the gas station.
The MPs would receive notice that a shooter was in the building and they had only that and their accumulated knowledge to fall back on.
Sergeant 1st Class Michael Brown, a 557th platoon sergeant, helped orgazine the training and he said it was more like Special Weapons and Tactics training than traditonal military work.
“The difference is that SWAT deals with barricaded subjects and hostages and here the focus is on active shooters,” he said.
Brown had his Soldiers start with solo missions because, he said, “We want them to go through by themsleves to get over their nervousness and to give them the confidence to go through.”
Knowing that a gunman was on the loose was all the knowledge Soldiers such as Pfc. Khalid Abbady had to work with.
“I was told to respond to an active shooter in the building,” said Abbady, a 557th medic. “This guy could be anywhere. There were seven to eight casualties in there.”
The notional casualites were picked for the role because they had no MP training, which Brown said “adds a more realistic effect.”
Some of the role-players were lying motionless and splattered in paintball explosions, while others went screaming from a room, panicking. Still, they managed to let Abbady know where the shooter was holed up.
“There were seven to eight casualties in there. Some were wounded and some were dead,” Abbady recalled. “I knew he was on the last door on the right. I went in quickly and tried to neutralize the threat. I went directly to the door and I went in and I got hit.”
Abbady continued to work on taking out the threat, only to get attacked by a second shooter.
But that makes for good training, he realized.
“It’s been a huge help and has been realistic,” Abbady said. “It changes everything when somebody’s in there. I learned not to fixate on one shooter because there could be more. You have to watch out for everything. And don’t leave your back to the door. I got it from behind.”
Heydon echoed Abbady’s sentiments about the training’s quality.
“I’ve done similar things, but not this in depth. This is my first paintball training ... and it’s more related to getting people out alive as opposed to room clearing where your just out to kill the person.”
Heydon described what happened when his turn came.
"I got the call, got out of my vehicle on the safe side, and radioed to get all the information I could,” he said. “I learned there was a guy on the first floor, so I went in there and heard a lot of people yelling. I was clearing a room when six or seven people came out from the hallway and told me where the shooter was.”
Falling back on his training and instinct, Heydon entered the room and got shot in the arm, but continued and elmininated the threat.
“But I got shot by the second shooter,” he said. “Then I eliminated him and finished the search.”
Sergeant David Banikci, a 557th team leader, said the training was “a lot better from the all-around aspect. Everying downrange is focused on the tactical side. Here in garrison, you’re going to act competely different than a tactical situation.”
For more information on U.S. Army Garrison Humphreys and living and working in Korea visit: USAG-Humphreys' official web site or check out our online videos.
# Thaw competent cells (Lyocomp E. coli) on ice.
# During this time pre-chill sterile centrifuge tubes on ice.
# Gently tap competent cell containers to mix
# Aliquot bacterial suspension in pre-chilled tubes (100 µl per tube).
# To the aliquots add 1 µl of plasmid (RFP PUC19).
# Gently tap centrifuge tubes to mix
# Incubate 30 min on ice.
# During this time pre-heat water bath to 42°C (don’t use heating block).
# Incubate samples at 42°C in the water bath for 60s. Do not shake.
# Immediately chill samples on ice for 2 minutes.
# Add 0.95 ml of room temperature SOC.
# Plate 10 µl of bacterial suspension on antibiotic-containing agar plate. (filter sterilized Kanamycin100 ug/ml?).
# Transfer some bacteria to another antibiotic-containing plate using a loop.
# Incubate plates at 37°C for 18 h.
This is a 1997 BMW Z3. The story behind its transformation may / may not be revealed over the next few weeks. Watch this space! Note that both shots are "As Taken" with my Samsung Galaxy S10. No enhancement whatsoever!
It was surprisingly warm (-1C) but dark in the mountains. But, nevertheless, nothing can replace the feeling of being in nature...! Have a wonderful week, everyone!
Datamorphix.ai is an innovative self serve AI helps in digital transformation
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Part of my 'Transformation' series
He tries to sleep, to forget the pain of his past, those faces, those places and of those memories. He tries to think of how he can truly be free in his life... in mind and in body...
Catalina Restrepo, Director, Centre for the Fourth Industrial Revolution, Medellín, Colombia; James Njoroge Muchiri, Deputy Governor, Nairobi City County, Kenya; Jeff Merritt, Head of Centre for Urban Transformation; Member of the Executive Committee, World Economic Forum; Jonas Brunschwig, Consul General of Switzerland in San Francisco, Consulate General of Switzerland in San Francisco, USA; Jonathan Reckford, Chief Executive Officer, Habitat for Humanity (HFH) International, USA; Michiel Dijkman, Chief Executive Officer, Economic Board Utrecht, Netherlands; Noridah Abdul Rahim, Director-General, Ministry of Federal Territories of Malaysia; Patrick Marmen, Chief of Staff, Bureau du design, Ville de Montréal, Canada; Sarah Dennis-Phillips, Planning Director, San Francisco Planning Department, City of San Francisco, USA; speaking in Executive Roundtable: Driving High-Quality Urban Development session at the Urban Transformation Summit 2025 in San Francisco, USA, 21/10/2025, 13:30 – 17:00, 23 - City Hall. Room 201. Copyright: World Economic Forum