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I had great fun watching and photographing this Osprey fishing in a nearby pond. They will be moving on very soon.
First time I've done a series of sequence photos without a tripod. Didn't turn out too bad, if I do say so myself.
Sequence of my friend Troy Gray doing a long nosegrind in Reno, NV.
9 images masked together manually in photoshop.
I hope this is self explanatory - a set of sequences, the second 3 with surfactant added to the reservoir. The last shot shows the free flow of fluid as well.
A short video of an image sequence shot with my Canon 7D MkII from shooting several bursts at 10 fps of an eagle grabbing a fish from the Mississippi River. The clip includes 30 of the 50 images shot, played at 10 fps. I got several nice shots out of this particular sequence
高島平団地です。連続するエレベータ室が素敵です。Tkashima-daira Apartments. SONY a7 + Carl Zeiss(sony) Sonnar FE 35mm F2.8 ZA
Free photographs of sequences to use for any reason. Check out TheWriteMoms.com for more free stuff including photoshop patterns of these.
These bands tell me what’s inside the genes of the plants I’m growing, isn’t it awesome?
I’m going to try to explain it (just to practice my written english):
I cut one leaf of each one of “my plants”, and put them inside different tubes. Then I grind and “cook” the leaf with some liquids, following a simple recipe, to extract the DNA. At the end (less than 1 hour with 40 samples and I’m still quite slow), I obtain a white gelatinous stuff in tubes, the DNA of each leaf.
Then I place a drop of this DNA (0,2 μL, nothing!!) that belongs to each leaf in small PCR-tubes with a “reaction mix” that contains an enzyme able to make DNA copies (borrowed from a bacteria that lives in hot spots), and something called “primers”, let's explain what they are:
As everybody knows, DNA is a double helix. In order to make a copy, both strands have to be separated (e.g. by heating), and then the enzyme copy every single strand in a way that it results in a new double helix, with the old strand used as a mold and a 'brand new strand'.
But the enzyme can’t just start copying a strand without a small amount of 'double helix sequence' already conformed, that’s why we need the primers. They are short sequences of “single strand” that complements (and therefore binds to) a specific region in the genome (the one you want to gossip). As they bind, they conform a short double strand at this point, and once the enzyme has this “starting point” is able to makes copies and copies and copies of that precise DNA region.
It’s necessary to heat and cool the reaction mix for every copy-cycle, so I place the tubes (with the DNA, the enzyme, the primers and the molecules the enzyme needs to build the DNA sequence) in a machine that heats&cools the tubes in cycles. In 2,5 hours (40 cycles) I get a few drops (20 µl) with thousands of copies of the sequence chosen by the primers and amplified by the enzyme.
The next step is to find out what’s inside the gene I’m interested in, and the easiest way to do it is: look at the size!! If the amplified sequence has the right size, there’s nothing weird in that plant, it’s “wildtype”. But we work with mutant plants bearing insertions on the genes that makes them useless to do their job (this is, to build proteins). So, if the sequence is too long (or just if there’s just not amplified sequence because the enzyme can’t copy a very long sequence) that means an insertion inside, hence this plant is a “knockout” for this gene. In other words, this gene doesn’t work in this plant.
How do I find out the length? easy: I pour the liquid with the amplified sequence inside a gel (each sample inside a different well) and apply an electric field. The DNA starts literally running towards the + electrode (because the DNA molecule has negative charge), but the bigger the fragment the slower it will move, due to the density of the gel. After less than half an hour, fragments with different lengths form bands at different levels. This is the moment to take a beautiful picture.
My goal now is to get a single plant with 2 knockout genes. In order to obtain the "double mutant", we cross 2 plants, each one with a single mutation, and wait for the seeds to grow… but, who knows which one of the children inherited both mutations? just cutting a single leaf and looking at the length of these amplified sequences.
You can see the result in the b/w picture with rows of bands over my lab-book. Each row belongs to a leaf (of a single plant), and each band to an amplified sequence. Depending of the position of the bands, I know the length of the sequence and therefore what’s happening inside of "her" DNA.
And everything in one single morning, definitely awesome!
PS. Thanks to my reviewers for helping me so much!!
Free photographs of sequences to use for any reason. Check out TheWriteMoms.com for more free stuff including photoshop patterns of these.
blog: Bikers and the Falkirk Wheel
The 12-shot sequence above was what this set up was intended for. Needs to be viewed at full size to appreciate properly. If you're wondering, there was a very... "Scottish"... change in the weather between the first and second shots, which is why the light's all wrong.
Top row, left to right:
#1 A barge sails into the top level of the Wheel.
#2 As the barge at the top is secured, a second barge sets off from the jetty next to the tourist centre (the domed building on the left) and sails into position.
#3 The barge in the lower section is almost in position. The barge at the top is ready for its spin.
Second row, left to right:
#4 Both boats are in position and secured. All gates have been shut.
#5 The Wheel begins to turn, in a clockwise direction as seen from end-on.
#6 About a quarter of the way round, and both boats are well clear of the waer they sailed from.
Third row, left to right:
#7 About halfway round.
#8 About two-thirds of the way round. The shape of the Wheel can be seen here; it's meant to echo the shape of an ancient Gaelic axe.
#9 Three quarters of the way round, and the bottom "blade" is swinging out of sight.
Bottom row, left to right:
#10 Almost in position...
#11 The Wheel has completed its 180 degree rotation, and the boats can now be untethered.
#12 The two boats sail out of the Wheel, on their respective ways.
A sequence of shots showing the partial phase of the total lunar eclipse of 27th/28th September 2015. Viewed from the UK.
This is an ABI 3130xl Genetic Analyser, in other terms a machine for DNA sequencing. The system is quite simple: polymer filled capillaries (the small coper colored tubes on the right) are used to separated DNA fragments according to their size. Short fragments will go through the capillaries faster than the longer ones. In the middle of the machine (where the capillaries go to) is a detector, a laser source that through emission and reception of light signal will identify which nucleotide is passing in front of it. On the computer attached to that sequencer we can then collect the DNA sequence, a mix of 4 letters: A, T, C, G. Looks like this: AATGGGCTTACGGCGCAAAAAG... Etc.
Practicing mild yoga exercise sequences is a fantastic method for novices to find out the positions. Gentle yoga likewise functions as a less complex and safer alternative to even more vigorous ranges. Gentle Warmup Flow This initial sequence is a great choice if you are brief in a timely...
paper : 13 * (9+3√3)
Paper of (9+3√3)*(9+3√3) is also OK by folding an extra strip backwards.
Prove here:
www.flickr.com/photos/119967028@N08/42341889754/in/datepo...
Division Method here:
www.flickr.com/photos/119967028@N08/42157641305/in/datepo...
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