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Characterization and phylogenetic analysis of HoblOBP3 and HoblOBP4.(A-B) The ORF of nucleotide sequence and deduced amino acid sequence of the OBP3 (A) and OBP4 (B) from H. oblita. The six conserved cysteines are indicated in rings with red color. The predicted signal peptide is underlined. The asterisk with red color marks the translation-termination codon. (C) Alignment of some OBPs amino acid sequence from Coleoptera insects. (D) Alignment of amino acid sequence between HoblOBP3 and HoblOBP4. (E) Phylogenetic tree of OBPs amino acid sequences in Coleoptera, including HoblOBPs. The corresponding OBPs in alignment and phylogenetic tree are listed as follow. RpalOBP2 (AAD31875, Rhynchophorus palmarum), DponOBP (AFI45058, Dendroctonus ponderosae), TcasOBP09 (EFA10713, Tribolium castaneum), TcasOBP23 (EFA10803, Tribolium castaneum), TcasOBP07 (EFA04593, Tribolium castaneum), HparOBP (AEA76516, Holotrichia parallela), HparPBP1 (ADF87391, Holotrichia parallela), HparOBP1 (BAC07272, Holotrichia parallela), HpicOBP1(BAC07270, Heptophylla picea), HpicOBP2 (BAC07271, Heptophylla picea), PjapPBP (AAC63436, Popillia japonica), EoriPBP (BAB70711, Exomala orientalis), AosaPBP (AAC63437, Anomala osakana), PdivOBP1 (BAA88061, Phyllopertha diversa), PdivOBP2(BAA88062, Phyllopertha diversa), AcupPBP1 (BAC06496, Anomala cuprea), ArufPBP (BAF79995, Anomala rufocuprea), ArufPBP2 (BAF91329, Anomala rufocuprea), AschPBP(BAF79599, Anomala schonfeldti), AschPBP2 (BAF79600, Anomala schonfeldti), MaltOBP1 (ABR53888, Monochamus alternatus), HoblOBP1(ACX32050, Holotrichia oblita), HoblOBP2(ACX32049, Holotrichia oblita), HoblOBP3 (ADX96030, Holotrichia oblita), HoblOBP4 (ADX96031, Holotrichia oblita).
See also: www.ludgerphilips.org/en/gallery/images-of-synthesis/
08 August 2014, pencils and photo work
Sagittarius is the fiery sign of the archer, symbol of single-minded attention. It teaches us to direct all our forces to one purpose, the goal of our life. On the spiritual path, the bow and arrow stand for the meditative discipline of lifting up the energies to the higher centres and to align one’s own will to the divine Will.
For the visualisation of the divine Will I chose a representation of the Lord of the Divine Will: I took the face of a statue of Shiva on the banks of the River Ganges in Rishikesh, India. I cleared it from other symbolisms and placed a bow and an arrow over the bust. With the help of photos of the sun and of a lotus I created the Sahasrara, the thousand-petalled lotus over the head, the target of the alignment, the connection to the higher worlds.
For the string and the lower part of the bust I created shapes of bluish flames, expressing the electric tension of the fiery alignment. I emphasized it by intensifying the colours.
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I know it's november when my satellite dish casts a shadow on the X on my shed. Since I don't have a satellite subscription that's about all it's good for.
After a good series of storms came through the area, I decided to get out and photograph a small section of the North End of the NorthWestern Pacific Railroad. I stayed within the Cloverdale, CA., area but these are the various stages of decay of the railroad.
©FranksRails Photography, LLC.
** Being used at <a href="http://www.sacred-destinations.com/france/carnac-stones.htm">www.sacred-destinations.com/france/carnac-stones.htm</a>
Ég hafði keppnina "skakkar myndir" á LMK í huga þegar ég tók þessa en sendi hana ekki inn svo hér er hún...
This manly-man saw that George lent me even had a laser guided sight. It came in very handy once you started cutting and the clouds of dust and sparks took over.
came without manual. Albeit the usage of the most tools is obvious to me some are not. A manual would be great - if you have a copy of the manual please contact me!
Almost done.
2005 Chevy 2500HD, LT, Crew Cab, Duramax / Allison, Short Box, 4x4, Pro Comp 6" Lift, 35" Mud Grapplers, 20" Mojave Teflon Wheels.
Twice a year, the sun sets in perfect alignment with Scripps Pier in San Diego, CA. Today, was one of those days.
Saturday morning. 8:40 am. 20 degrees F.
During the 4 minutes between dropping the boys at the soccer fields for their games and...
...hitting the ground running at work, not stopping to eat or pee or anything for about 9 hours.
I forgot all about this in-between moment until tonight, Sunday evening, slipping the photocard into the laptop.
Going to go take an all-night nap.
I decided to try my hand at doing my own alignments! I've done alignments before, but only front toe, and not in a very long time. On my Civic, toe and camber are adjustable front and rear, and I've already adjusted the caster with the bushings I have in the front control arms. Although it wasn't difficult to do, it was rather tedious, and getting the toe right on the driver's side front took a lot of trial and error. See my results photo for how I did!
Phylogenetic affiliation of sequences retrieved using cyanobacterial specific nifH genes as targets combined with DGGE analyses of sediment associated DNA samples from wetland meadows along three major rivers (Vindel, Pite, and Lais) in northern Sweden.The phylogram was constructed using the maximum likelihood distance method with TrNIG model. Sequences obtained here are given in bold. Each DGGE band sequence is designated by their accession number, a code and the name of the river/s were it was collected. The numbers given at the nodes represent bootstrap values.
When the long road bridge which spanned Galashiels railway station was demolished and replaced by a re-aligned road, an opening was left in the new road embankment for use in the event that trains might one day return to the Waverley Route. This view looks towards Edinburgh. Obviously a new alignment for the railway was envisaged here. The building which blocked the route on the other side of this bridge can be seen in old photos as lying to the north of the railway and has since been demolished to make way for the new rail alignment..
Rimmy Jims, Arizona.
Route 66 (Alignment 1926-1950s).
Near ruins of Meteor Crater Observatory.
Pierre and Julie.
Près des ruines de Meteor Crater Observatory.
Pierre et Julie.
Passenger door fitted up pretty much without a hitch.
I scribed marks on the pillars before removing hinges a while back.
success! i've managed to rip out the portion of Sensor that does the registration.
now to massively refactor the code so it can actually be included in libfreenect...
Domain structure, phylogeny and conservation of critical catalytic features of ciliate PI4Ks.A, Domain structure of Tetrahymena PI4Ks. The RING domain predicted in PI4K2 has been removed (see Table S1 and Methods). Domain boundaries, e-values and further details are given in Table S1. B, Unrooted neighbor-joining tree of catalytic domains from 28 ciliate, 2 yeast (Stt4, Pik1) and 2 mammalian (PI4Ka and PI4Kb) type III PI4Ks. Bootstrap values from 1000 replicates higher than 70% are indicated near the corresponding branches. Type III?, III? and ciliate-specific III-like PI4Ks are color coded (blue, green and red, respectively). Circles and triangles represent Tetrahymena and Paramecium PI4Ks, respectively. Bar indicates number of amino acid substitutions per site. C, Sequence alignment of the catalytic kinase domains from type III ciliate, mammalian and yeast PI4Ks. The position of prominent catalytic features is indicated by arrows and numbered residues refer to human PI4KIII? sequence [58]. All ciliate PI4Ks have conserved catalytic motifs and they bear key residues: the K1792 residue (IFK motif), the catalytic motifs DRH1901-N1904 and HID1917 and the E1933 and K1937 residues in the activation loop [58]. Significant conservation was also observed in the G-loop (G1836 in human PI4KIII?). The TtPI4K1 and PtStt4 G-loop (highlighted in red) could not be properly aligned due to a unique 5aa insertion in both PI4Ks. Also, alignment of the activation loop and the E1933 and K1937 residues was distorted due to unique insertions in all type III-like ciliate PI4Ks (highlighted in grey). PtPI4K21 apparently lacks a portion of the activation loop.