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My painting for Dirty Footprints Studio Total Alignment exercise 1 blogged

Taken from the ferry between beautiful city of Nynäshamn and the beautiful island of Nåttarö (also spelled Nåtarö, sometimes) in the Stockholm archipelago..

On a nice autumn walk a few years ago

The moon, the peak of the sunlit cumulus cloud and Moel Famau's summit line up as the sun rose and I headed to the top of Halkyn Mountain.

Pentax K20D • Sigma 17-70mm f:2.8-4.5 DC Macro

 

St Peter Church - Paris - France

Alignment tool for mounting an idicator to align direct driven equipment to the driver.

DCIM\100GOPRO Bypassed 2010

I'm pleased to announce that this image and the one below, "Pipe Dreams", have been nominated in the Photography Masters Cup 2012. This one in the Fine Art Category.

DCIM\100GOPRO Manning River Bridge

lights perspective in Orta, Italy

Analysis of primary structure and conserved domains of SGT1 proteins.(A) Sequence alignment analysis and the predicted conserved domains of SGT1 proteins. GenBank accession numbers: Rice (AAF18438), Wheat (EF546432.1), Arabidopsis (AF439975, AF439976), Barley (AF439974). The black (100%), pink (80%), and blue (60%) boxes represent levels of amino acid identity or similarity. The conserved domains were underlined. (B) The phylogenetic analysis of the amino acid sequence of SGT1 genes from different species. GenBank accession numbers: OsSGT1 (AAF18438), TaSGT1 (ABQ23992.1), TaSGT1-1 (ABO18602.1), TaSGT1-2 (ABO18603.1), HHvSGT1-Barley (AF439974), BdSGT1 (XP_003569394.1), SbSGT1 (EES01101.1), Zm-SGT1 (ACG34278.1), NbSGT1 (AAW82048.1), AtSGT1a (AF439975) and AtSGT1b (AF439976). The tree was generated by ClustalX1.83 analysis with the corrected full-length Hv-SGT1 protein sequences using Neighbor-Joining method (MEGA4.0 software). The bar beneath the dendrogram represents a distance of 0.05 change per amino acid.

Influence of alignment filtering.(A) Random filtering and phylogenetic filtering both remove sequences from the unfiltered alignment, which is represented by the large tree a, but result in trees (b and c) that differ in the length of terminal branches (red). Tree b (random filter) is similar to a in containing many extremely short terminal branches that are known to challenge coevolution detectors. In contrast, tree c (phylogenetic filter) lacks short terminal branches. (B) Opposing effects of progressively increasing strength of filtering, which leaves gradually fewer sequences in the alignment. The top graph shows, for the phylogenetic filter, the minimal sequence-sequence distance among all sequence pairs in the filtered alignment. The two lower graphs show performance, measured by , of a coevolution detector for both the phylogenetic and random filter. The first effect, specific to the phylogenetic filter, is a rise of with increasing strength of filtering (decreasing number remaining sequences). This reflects the disappearance of short terminal branches, which in turn improves performance, until a maximum is reached around 250 sequences remaining. The second effect is the deterioration of performance with increasing strength of filtering, since fewer sequences provide less information for the coevolution detector. This effect is clearly seen for the random filter regardless of the number of remaining sequences but it becomes apparent for the phylogenetic filter only with strong filtering. Conditions: detector  =  MIp; protein family  =  ABC-C. Trees were plotted using FigTree v1.3.1 (tree.bio.ed.ac.uk/software/figtree/).

pen & ink, and watercolour on paper

Today I was on the 174 just passed Blair Rd. heading west and stopped to get a shot of this track alignment machine that is working on the city of Ottawa's LRT project. There was another one there but to many trees in the way.

The consensus sequences responsible for Ada protein specific interaction (A) located in promoter sequences (up to -100 nucleotide residues relative to ATG start codons) of E. coli ada, alkA, and aidB.Additionally, the predicted consensuses in promoter regions of E. colialkB and P. putidaalkA, gcdH, alkB, and ada genes are shown (green bars). Also, the putative -35 and -10 boxes responsible for RNA polymerase interaction are marked in pink (retrieved from RegulonDB, regulondb.ccg.unam.mx) or grey (SoftBerry BPROM prediction tool, linux1.softberry.com). The phylogenetic tree (B) shows relative conservation between depicted consensus sequences based on UPGMA Geneious Tree Builder, A box – light blue, B box – brown, numbers indicate substitutions per site (for details see Materials and Methods).

On board unit 4018 as we run along the shared alignment with Network Rail, both running lines here between Pelaw and Jarrow are single track, the disused freight line on the left goes to the now closed Shell Oil storage facility at Jarrow, whilst i am on a service for South Shields, on the Tyne and Wear Metro system's Yellow Line, having not ridden this system before it was a rather nice surprise to be offered a drivers eye view that you have on these 1978/81 Metro-Cammell Birmingham built units, of which there are 90 two car articulated units, which usually operate in pairs.

Maybe in the future the disused freight line could converted to become part of the Metro system to form a double track section as most of the Metro system is.

Nikon FG - 2008

Kodachrome 64

Athens, GA - Clarke County

 

A very familiar scene here in my photostream... but this time, in Kodachrome.

Workforce Alignment workshop "Building strong partnerships to support Wisconsin’s workforce need." A conversation hosted by UW Oshkosh, Department of Workforce Development, Fox Valley Tech and WAICU.

Multiple alignment and phylogenetic analysis of EsMYBA1 and related plant R2R3-MYB proteins known to regulate the flavonoid pathway.(A) Alignment of deduced amino acid sequences of EsMYBA1 and other plant R2R3-MYB proteins. Identical amino acid residues are shaded in black, similar in gray. The R2 and R3 domains shown refer to two repeats of the MYB DNA-binding domain of selected MYB proteins. Three conserved motifs, the bHLH interaction motif, the ANDV motif identified in Rosaceae and the motif 6 from Arabidopsis R2R3-MYB family classification are boxed. The two arrowheads indicate the insert position of intron I and II, respectively. (B) Phylogenetic tree of EsMYBA1 and selected R2R3-MYB proteins from other plant species using the neighbor-joining method by the MEGA 5 software. The scale bar represents the number of substitution per site and the numbers next to the nodes are bootstrap values from 1,000 replicates. The EsMYBA1 are indicated as a diamond. The putative regulatory functions of the different R2R3-MYB proteins in the control of phenylpropanoid biosynthesis pathway are indicated. All R2R3-MYB protein sequences were retrieved from GenBank database and accession numbers are as follows (in parentheses): Antirrhinum majus AmROSEA1 (ABB83826); AmROSEA2 (ABB83827); AmVENOSA (ABB83828); Arabidopsis thaliana AtPAP1 (AAG42001); AtPAP2 (AAG42002); AtTT2 (NP_198405); AtMYB12 (ABB03913); AtMYB4 (NP_195574); Citrus sinensis CsRuby (AFB73913); Diospyros kaki DkMYB4 (BAI49721); Fragaria x ananassa FaMYB1 (AAK84064); Garcinia mangostana GmMYB10 (ACM62751); Gerbera hybrid GhMYB10 (CAD87010); Ipomoea batatas IbMYB1 (BAF45114), Ipomoea nil InMYB2 (BAE94709); Lycopersicon esculentum (Solanum lycopersicum) LeANT1 (AAQ55181); SlMYB12 (ACB46530); Lilium hybrid LhMYB6 (BAJ05399); Lotus japonicus TT2a (BAG12893); Malus x domestica MdMYB10a (ABB84753); Medicago truncatula MtLAP1 (ACN795410; Morella rubra MrMYB1 (ADG21957); Nicotiana tabacum NtAN2 (ACO52470); Oryza sativa OsMYB4 (BAA23340); Petunia x hybrida PhAn2 (AAF66727); Vitis vinifera VvMYBA1 (BAD18977); VvMYBA2 (BAD18978); VvMYBPA1 (CAJ90831); VvMYBPA2 (ACK56131); VvMYBF1 (ACV81697); VvMYB5a (AAS68190); VvMYB5b (AAX51291); Zea mays ZmC1 (AAA33482); ZmPl (AAA19819).

Alignment tool for mounting an idicator to align direct driven equipment to the driver.

Workforce Alignment workshop "Building strong partnerships to support Wisconsin’s workforce need." A conversation hosted by UW Oshkosh, Department of Workforce Development, Fox Valley Tech and WAICU.

alignment and check on track platform configuration

With Dduallt station just out of sight this was the original alignment of the Ffestiniog Railway between Dduallt and Tanygrisiau, before the building of the 'Great Deviation' route to raise the level of the line above Tanygrisiau reservoir.

 

22nd October 2017

Amboy, California.

Route 66 (Alignment 1926-1972) - National Trails Hwy.

 

Roy's Motel, Cafe & Station (1938) was named after Roy Crowl. Roy passed in 1977. Roy's was formerly known as Bender's Cottages, which predated 66 in the early 1920's along the National Old Trails Hwy.

The huge Googie styled neon sign was added in 1959.

In 2005, Albert Okura, owner of the Juan Pollo Restaurant chain in California, has restored Roy's.

The ambiance of Roy's is poignant throwback to the heyday of Route 66. (5)

 

Le Motel Roy, Cafe & Station (1938) a été nommé d'après Roy Crowl. Roy est décédé en 1977. Le Roy's était anciennement connu sous le nom de Bender's Cottage, qui est existait avant la "66", au début des années 1920, le long de la National Old Trails Hwy.

La grande enseigne au néon de style Googie, a été ajoutée en 1959.

En 2005, Albert Okura, propriétaire de la chaîne des Juan Pollo Restaurant en Californie, a restauré le Roy's.

L'ambiance du Roy's est poignante et nous ramène à l'apogée de la Route 66. (5)

 

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

Canon AE1

Graceland Cemetery, Chicago

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

The reference phylogenetic tree and evolution of mitochondrial intergenic spacers.Maximum likelihood tree (?ln?=?44305.147421) inferred from the aag13sp-set ALN. The analysis was performed by applying the GTR+G evolutionary model and according to the most complex partitioning scheme described in the main text. Blue-coloured numbers indicate bootstrap values expressed as percentage, whereas red-coloured numbers indicate posterior probabilities computed through Bayesian inference analysis on the same data set. The scale bar represents 0.1 substitutions/site. Occurrence and evolutionary pathway of isps exhibiting a uniqueness in term of genomic position plus sequence identity. Multiple alignments of isps, representing unique molecular signatures, are provided with invariant positions depicted on a blue background. Red/pink background is used to identify nucleotides characterizing peculiar clades. Single sequences/multiple alignments of isps, not representing unique molecular signatures, are depicted on a purple background. The inset on the bottom shows the placement of isps in the lepidopteran complete mitochondrial genome. Genes are coloured as in Figure 1.

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

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