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Workforce Alignment workshop "Building strong partnerships to support Wisconsin’s workforce need." A conversation hosted by UW Oshkosh, Department of Workforce Development, Fox Valley Tech and WAICU.

Multiple alignment and phylogenetic analysis of EsMYBA1 and related plant R2R3-MYB proteins known to regulate the flavonoid pathway.(A) Alignment of deduced amino acid sequences of EsMYBA1 and other plant R2R3-MYB proteins. Identical amino acid residues are shaded in black, similar in gray. The R2 and R3 domains shown refer to two repeats of the MYB DNA-binding domain of selected MYB proteins. Three conserved motifs, the bHLH interaction motif, the ANDV motif identified in Rosaceae and the motif 6 from Arabidopsis R2R3-MYB family classification are boxed. The two arrowheads indicate the insert position of intron I and II, respectively. (B) Phylogenetic tree of EsMYBA1 and selected R2R3-MYB proteins from other plant species using the neighbor-joining method by the MEGA 5 software. The scale bar represents the number of substitution per site and the numbers next to the nodes are bootstrap values from 1,000 replicates. The EsMYBA1 are indicated as a diamond. The putative regulatory functions of the different R2R3-MYB proteins in the control of phenylpropanoid biosynthesis pathway are indicated. All R2R3-MYB protein sequences were retrieved from GenBank database and accession numbers are as follows (in parentheses): Antirrhinum majus AmROSEA1 (ABB83826); AmROSEA2 (ABB83827); AmVENOSA (ABB83828); Arabidopsis thaliana AtPAP1 (AAG42001); AtPAP2 (AAG42002); AtTT2 (NP_198405); AtMYB12 (ABB03913); AtMYB4 (NP_195574); Citrus sinensis CsRuby (AFB73913); Diospyros kaki DkMYB4 (BAI49721); Fragaria x ananassa FaMYB1 (AAK84064); Garcinia mangostana GmMYB10 (ACM62751); Gerbera hybrid GhMYB10 (CAD87010); Ipomoea batatas IbMYB1 (BAF45114), Ipomoea nil InMYB2 (BAE94709); Lycopersicon esculentum (Solanum lycopersicum) LeANT1 (AAQ55181); SlMYB12 (ACB46530); Lilium hybrid LhMYB6 (BAJ05399); Lotus japonicus TT2a (BAG12893); Malus x domestica MdMYB10a (ABB84753); Medicago truncatula MtLAP1 (ACN795410; Morella rubra MrMYB1 (ADG21957); Nicotiana tabacum NtAN2 (ACO52470); Oryza sativa OsMYB4 (BAA23340); Petunia x hybrida PhAn2 (AAF66727); Vitis vinifera VvMYBA1 (BAD18977); VvMYBA2 (BAD18978); VvMYBPA1 (CAJ90831); VvMYBPA2 (ACK56131); VvMYBF1 (ACV81697); VvMYB5a (AAS68190); VvMYB5b (AAX51291); Zea mays ZmC1 (AAA33482); ZmPl (AAA19819).

Workforce Alignment workshop "Building strong partnerships to support Wisconsin’s workforce need." A conversation hosted by UW Oshkosh, Department of Workforce Development, Fox Valley Tech and WAICU.

With Dduallt station just out of sight this was the original alignment of the Ffestiniog Railway between Dduallt and Tanygrisiau, before the building of the 'Great Deviation' route to raise the level of the line above Tanygrisiau reservoir.

 

22nd October 2017

Amboy, California.

Route 66 (Alignment 1926-1972) - National Trails Hwy.

 

Roy's Motel, Cafe & Station (1938) was named after Roy Crowl. Roy passed in 1977. Roy's was formerly known as Bender's Cottages, which predated 66 in the early 1920's along the National Old Trails Hwy.

The huge Googie styled neon sign was added in 1959.

In 2005, Albert Okura, owner of the Juan Pollo Restaurant chain in California, has restored Roy's.

The ambiance of Roy's is poignant throwback to the heyday of Route 66. (5)

 

Le Motel Roy, Cafe & Station (1938) a été nommé d'après Roy Crowl. Roy est décédé en 1977. Le Roy's était anciennement connu sous le nom de Bender's Cottage, qui est existait avant la "66", au début des années 1920, le long de la National Old Trails Hwy.

La grande enseigne au néon de style Googie, a été ajoutée en 1959.

En 2005, Albert Okura, propriétaire de la chaîne des Juan Pollo Restaurant en Californie, a restauré le Roy's.

L'ambiance du Roy's est poignante et nous ramène à l'apogée de la Route 66. (5)

 

You can fiddle around with vernier gauges on either side of your rim. I prefer a custom cut piece of stainless rod accurately located using the valve hole in the rim to asses the wheel for being central. The wheel must be perfectly dished.

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

Canon AE1

Graceland Cemetery, Chicago

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

The reference phylogenetic tree and evolution of mitochondrial intergenic spacers.Maximum likelihood tree (?ln?=?44305.147421) inferred from the aag13sp-set ALN. The analysis was performed by applying the GTR+G evolutionary model and according to the most complex partitioning scheme described in the main text. Blue-coloured numbers indicate bootstrap values expressed as percentage, whereas red-coloured numbers indicate posterior probabilities computed through Bayesian inference analysis on the same data set. The scale bar represents 0.1 substitutions/site. Occurrence and evolutionary pathway of isps exhibiting a uniqueness in term of genomic position plus sequence identity. Multiple alignments of isps, representing unique molecular signatures, are provided with invariant positions depicted on a blue background. Red/pink background is used to identify nucleotides characterizing peculiar clades. Single sequences/multiple alignments of isps, not representing unique molecular signatures, are depicted on a purple background. The inset on the bottom shows the placement of isps in the lepidopteran complete mitochondrial genome. Genes are coloured as in Figure 1.

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

You just start without knowing what's going to happen. I had fun this time blending colours on the surface. blogged

These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.

 

The fit up needed to be very close, so we were working to within a few tenths.

 

The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.

   

A detail of the next photo, I had to lighten up a lot this group which was playing just under a weak, bleak, blinking neon light. The musicians were happy to be taken in photos and not paying any attention to me, only concentrated on what they were doing - well, this was serious work, of course.

 

There is a fantastic place where groups are rehearsing regularly, along the huge Av. de Bélgica (NB : funny that my little country has such a big avenue with her name, while Brazil, for instance, has only a very narrow - but charming - little street in the middle of Old Havana, LOL !)

blogged

 

Started off by prepping the paper with gesso just to give it a bit more tooth (the paper I'm using is a bit cheap).

 

Wrote words with a 3B pencil all over. Started with the big red shapes, then added some green.

 

Decided (probably too consciously) to do a figure. Added more green around her head.

 

Got stuck quite a few times, then hit on lilac coloured hair. Got stuck again, decided to make her body white.

 

Struggled to know what to do, so eventually just stopped.

 

I found this a lot harder than abstract as I just didn't know where to go with it.

 

Still enjoyed it though!

The title is alignment all the way to the left. The two rows are aligned to the left, so it has the 'pleasant-look'.

Alignment tool for mounting an idicator to align direct driven equipment to the driver.

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

Inscribed rock at the foot of Spencer Trail

 

Spencer Trail was built in 1910 by Charles Spencer's crew. It followed the cliff face north of the Colorado River and was constructed using picks, shovels, and crowbars. The trail leads to coal deposits that were hauled to the ferry site by pack mule and used to fuel the mining machinery.

 

One report claims that the trail followed the route of a former Native Americn trail. Another account suggests that Spencer's mule "Pete" marked the trail alignment after Spencer gave him instruction on what to do.

  

Lees Ferry and Lonely Dell Ranch Landscape

Glen Canyon National Recreation Area

This abandoned alignment is on the property of Traders Point Christian Church. The remains of a building foundation stretch across the road. Historic aerials show that this segment has been bypassed since the 1950s, and that a small building was once built here.

These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.

 

The fit up needed to be very close, so we were working to within a few tenths.

 

The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.

   

Check it out for a weld sequence.

 

www.engincycles.com

Each of the pointed corners is aligned with the one below it... All the corners form a straight line!

New York State Route 27 - Old Alignments (Old Montauk Highway in Montauk)

Shots the next day of our KULAY Workshop in Reem Riviera Resort. OFW month event

 

Comments and Criticism are welcome

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