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Sequence, phylogeny and structural analysis of Sc-KU-4.A: Amino acid sequence alignment of the Kunitz domain in Sc-KU-4 with the homologous domains of other nematode Kunitz-like inhibitors. Conserved positions are shaded; the solid lines indicate the conserved disulfide bridges, and the discontinuous line indicates the missing CysII–CysIV bridge in Sc-KU-4. B: Phylogenetic analysis showing Sc-KU-4 closes to other nematode Kunitz-like inhibitors forming a separate cluster with A. suum. C: Sc-KU-4 structure prediction based on multiple-threading alignments showing the Kunitz domain exposed on the molecular surface in a characteristic tertiary fold of three antiparallel β-strands followed by one α-helix. Cysteine residues responsible for reactive loop stabilization and glycine residues that replace CysII–CysIV are shown in gray. The discontinuous line box highlights the amino acids present in reactive loops. The P1 position is shown in green. Asi: Anisakissimplex; Asu: Ascarissuum; Cbn: Caenorhabditisbrenneri; Cbr: C. briggsae; Cel: C. elegans; Cre: C. remanei; Llo: Loaloa; Sca: S. carpocapsae.
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
Finally got a proper alignment today. We were able to get the following settings:
Front:
-2.3 degrees camber
6 degrees caster
0 toe
Rear:
-3.7 degrees camber
1/16" total toe in
It definitely drives like it's on rails now. The rear is planted very nicely during hard cornering. Yay!
This morning I observed the grand alignment of planets!
In general, alignments of planets are pretty common for us, but this year we could see a rare combination. All planets that we can see with naked eye (Mercury, Venus, Mars, Jupiter, and Saturn) are aligned this month in the same order as they lie from the Sun.
Meanwhile, there are Uranus and Neptune in the same area of the sky I could capture as tiny tiny dots in the picture. I’m not sure Instagram would even resolve them here, but I made some captions so that you know where planets are.
Unfortunately there is no Mercury in that picture. Well, it’s kind of in the frame though, but was covered by the trees at the time of capturing. However, I observed it with naked eye later this morning.
You can spot the bright Moon between Venus and Mars, and, in my opinion, it takes a cool position in the picture because it can represent “our planet” in the sky.
If you missed the alignment this morning, you still have some time to look at it during the next few days.
Characterization and phylogenetic analysis of HoblOBP3 and HoblOBP4.(A-B) The ORF of nucleotide sequence and deduced amino acid sequence of the OBP3 (A) and OBP4 (B) from H. oblita. The six conserved cysteines are indicated in rings with red color. The predicted signal peptide is underlined. The asterisk with red color marks the translation-termination codon. (C) Alignment of some OBPs amino acid sequence from Coleoptera insects. (D) Alignment of amino acid sequence between HoblOBP3 and HoblOBP4. (E) Phylogenetic tree of OBPs amino acid sequences in Coleoptera, including HoblOBPs. The corresponding OBPs in alignment and phylogenetic tree are listed as follow. RpalOBP2 (AAD31875, Rhynchophorus palmarum), DponOBP (AFI45058, Dendroctonus ponderosae), TcasOBP09 (EFA10713, Tribolium castaneum), TcasOBP23 (EFA10803, Tribolium castaneum), TcasOBP07 (EFA04593, Tribolium castaneum), HparOBP (AEA76516, Holotrichia parallela), HparPBP1 (ADF87391, Holotrichia parallela), HparOBP1 (BAC07272, Holotrichia parallela), HpicOBP1(BAC07270, Heptophylla picea), HpicOBP2 (BAC07271, Heptophylla picea), PjapPBP (AAC63436, Popillia japonica), EoriPBP (BAB70711, Exomala orientalis), AosaPBP (AAC63437, Anomala osakana), PdivOBP1 (BAA88061, Phyllopertha diversa), PdivOBP2(BAA88062, Phyllopertha diversa), AcupPBP1 (BAC06496, Anomala cuprea), ArufPBP (BAF79995, Anomala rufocuprea), ArufPBP2 (BAF91329, Anomala rufocuprea), AschPBP(BAF79599, Anomala schonfeldti), AschPBP2 (BAF79600, Anomala schonfeldti), MaltOBP1 (ABR53888, Monochamus alternatus), HoblOBP1(ACX32050, Holotrichia oblita), HoblOBP2(ACX32049, Holotrichia oblita), HoblOBP3 (ADX96030, Holotrichia oblita), HoblOBP4 (ADX96031, Holotrichia oblita).
The screw located behind the mirror is for rangefinder vertical alignment. It tilts the mirror forward or backward. The reflected rangefinder patch will be too high when the mirror tllts too backwards and vice versa.
From from top to bottom, you can see two bright objects: Venus and Jupiter. Two fainter objects are the star Regulus to the lower left of Venus and the planet Mars between Venus and Jupiter. The predawn hours of the mornings of October 7, 8, and 9 will have similar alignments. earthsky.org/tonight/moon-venus-and-jupiter-align-in-morn...
The ePHD/ADD domains of SPBP and RAI1 are conserved in evolution.(A) Alignment of the putative ePHD/ADD domain of SPBP and RAI1 in mammalia, reptiles, birds, amphibians, fishes and insect. Cysteine and histidine residues that may serve as zinc-ligands are indicated by numbers above the alignment. The long loop region between zinc ligands 2 and 3 in all species is excluded from the alignment, but indicated above. The regions encompassing the putative GATA-1 like finger and the PHD finger are indicated above, while the Loop1, Loop2 and Loop3 indicated below are loop structures generally found in PHD fingers. The conserved Tryptophan units used to select blasted sequences are indicated by arrows, while amino acids probably involved in Smith-Magenis syndrome are encircled. Two of the RAI1 sequences (A.carolinensis and L.chalumnae) are truncated in the C-terminal end probably due to sequencing errors. The threshold for shading in manually refined Clustal W sequence alignment was set to 50% using BLOSUM 62 scoring matrix. (B) Phylogenetic tree of the ePHD/ADD domains of SPBP and RAI1 proteins. The phylogenetic tree was constructed by Mega5 software using the maximum likelihood method. Numbers in the branch represents the bootstrap values. Background color coding is used to represent species which possess SPBP (blue), RAI1 (pink) and uncharacterized SPBP/RAI1 like proteins (green). The phylogenetic tree is based on selected protein sequences from representative vertebrate organisms (e.g. Homo sapiens represent Mammalia). (C) The evolutionary tree of the ePHD/ADD domains of SPBP and RAI1 proteins. The alignment of all sequences is shown in Supporting information, Figure S1 and the protein sequence accession numbers are provided in the Supporting information, Table S3.
Civilisation but how civilised it was in reality? Ballast stones left in the jungle and one by one going through emaciated hands carefully stapled to become a straight alignment for a train wanted by the Japanese Imperial Army for supplying stronghold Burma with goods and moral for their attempt to conquer India. Burma was as far as they came.
See also: www.ludgerphilips.org/en/gallery/images-of-synthesis/
08 August 2014, pencils and photo work
Sagittarius is the fiery sign of the archer, symbol of single-minded attention. It teaches us to direct all our forces to one purpose, the goal of our life. On the spiritual path, the bow and arrow stand for the meditative discipline of lifting up the energies to the higher centres and to align one’s own will to the divine Will.
For the visualisation of the divine Will I chose a representation of the Lord of the Divine Will: I took the face of a statue of Shiva on the banks of the River Ganges in Rishikesh, India. I cleared it from other symbolisms and placed a bow and an arrow over the bust. With the help of photos of the sun and of a lotus I created the Sahasrara, the thousand-petalled lotus over the head, the target of the alignment, the connection to the higher worlds.
For the string and the lower part of the bust I created shapes of bluish flames, expressing the electric tension of the fiery alignment. I emphasized it by intensifying the colours.
Drivetrain alignment looks correct based on my drawings. If it wasn't correct that would have ment I screwed up somewhere. It's all good.
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I know it's november when my satellite dish casts a shadow on the X on my shed. Since I don't have a satellite subscription that's about all it's good for.
Saturday morning. 8:40 am. 20 degrees F.
During the 4 minutes between dropping the boys at the soccer fields for their games and...
...hitting the ground running at work, not stopping to eat or pee or anything for about 9 hours.
I forgot all about this in-between moment until tonight, Sunday evening, slipping the photocard into the laptop.
Going to go take an all-night nap.