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Sequence alignment of knowpains. A.The deduced amino acid sequences of knowpain-2 (KP2), knowpain-3 (KP3), knowpain-4 (KP4), vivapain-2 (VX2), vivapain-3 (VX3), vivapain-4 (VX4), falcipain-2 (FP2), falcipain-3 (FP3) and bergheipain (BP2) were aligned by using the multalin program. Predicted transmembrane domains are underlined, and ERFNIN and GNFD inhibitory motifs in the prodomain are labeled with filled circles. The positions of mature domain processing sites are indicated by arrowheads, and the catalytic amino acids are indicated by asterisks. The refolding domain and haemoglobin-binding motif of FP2 are boxed and underlined, respectively. The table shows % sequence identities within the FP2/3 subfamily based on the entire prodomain-mature protease regions. B. The phylogram was generated using the Neighbor Joining algorithm from the sequence alignment of mature protease domains (beginning with the conserved DWR motif to the end) of papain-like proteases of parasitic Protozoa and their selected human homologs (cathepsin L, cathepsin K, and cathepsin S). The accession numbers for various proteases are: P. knowlesi proteases knowpain-1 (KP1, XP_002260291), knowpain-2 (KP2, XP_002259153), knowpain-3 (KP3, XP_002259152), knowpain-4 (KP4, XP_002259151); P. falciparum proteases falcipain-1 (FP1, XP_001348727, falcipain-2 (FP2A XP_001347836), falcipain-2? (FP2?, XP_001347832), falcipain-3 (FP3, XP_001347833); P. vivax proteases vivapain-1 (VX1, XP_001615807), vivapain-2 (VX2, XP_001615274), vivapain-3 (VX3, XP_001615273), vivapain-4 (VX4, XP_001615272); P. berghei proteases bergheipain-1 (BP1 XP_677643) and bergheipain-2 (BP2, PBANKA_093240); P. chabaudi chabaudi proteases chaubipain-1 (CP1, AAP43629) and chaubipain-2 (CP2, AAP43630); P. yoelii yoelii proteases yoelipain-1 (YP1, XP_729023) and yoelipain-2 (YP2, XP_726900); Babesia bovis proteases bovipain-1 (BV1, XP_001612131) and bovipain-2 (BV2, XP_001610695); Entamoeba histolytica cysteine protease-1 (EhCP1, Q01957), cysteine protease-2 (EhCP2, Q01958), cysteine protease-5 (EhCP5, CAA62835); Giardia lamblia cysteine protease-2 (GlCP2, EAA41050); Leishmania major cysteine protease-1 (LmCP1, AAB48119), cysteine protease-2 (LmCP2, AAB48120); Neospora caninum cysteine protease-1 (NcCP1, CCA30060) cysteine protease-2 (NcCP2, CBZ49954); Theileria annulata cysteine protease (TaCP, AAA30135); Toxoplasma gondii cysteine protease-1 (TgCP1, AAL60053), cysteine protease-2 (TgCP2, ABY58967); Trypanosoma cruzi cysteine protease-1 (TcCP1, AAA30181), cysteine protease-2 (TcCP2, AAC37213); human cathepsin L (HcathL), AAC23598), cathepsin S (HcathS, AAC37592) and cathepsin K (HcathK, P43235).
These three celestial wonders were in an almost perfect alignment when I took this photo during a cloudy twilight sky.
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
all the chinese characters are aligned onto the left, which makes this image looking as if it is very organized.
And this was another big reason. The bonnet hinge (front hinged) has a fair bit of flex in it so the bonnet closes in a slightly different position laterally every time it is shut. Not too much of a problem if the finish is a single colour, but a big problem as soon as you have a stripe through the finish. I just couldn't think of an elegant solution for a long while, got real fed up and gave up....!! Then, as often happens, you think about other stuff, do different things, and the solution suddenly becomes blindingly obvious. A taper on the extended shaft of the bonnet latch sliding through an alignment hole in an adjustable bracket underneath the latch striker plate ensures that the bonnet aligns correctly every time. Simple and reliable. Why didn't I think of that 6 months ago...!!
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
Park Tool makes some great product. However this base must have been made on a Friday. Rogue bolt hole... www.44bikes.com
Phylogenetic tree construction and multiple sequence alignment of Cactus proteins from various species.(A) Neighbor-joining phylogenetic tree analysis of the full-length amino acid sequences of Cactus proteins (LvCactus was marked with solid triangle) using MEGA 5.0 software; (B) Schematic representation and (C) Multiple sequence alignment (using clustal X v2.0 method) of the ankyrin repeat domains of Cactus proteins with the identical amino acid residues shaded in black and the similar residues in gray. Proteins analyzed list below: LvCactus, Litopenaeus vannamei Cactus (Accession No. JX014314); AmCactus, Apis mellifera Cactus 1 (Accession No. NP_001157184.1); AeCactus, Acromyrmex echinatior Cactus (Accession No. EGI65248.1); BmCactus, Bombyx mori Cactus (Accession No. NP_001166191.1); CfCactus, Camponotus floridanus Cactus (Accession No. EFN66754.1); CeIκB-1, Caenorhabditis elegans IκB-1 (Accession No. NP_492575.1); DmCactus1, Drosophila melanogaster Cactus isoform A (Accession No. AAN10936.1); DmCactus2, Drosophila melanogaster Cactus isoform B (Accession No. NP_476942.1); DpCactus, Daphnia pulex Cactus (Accession No. EFX89207.1); HsIκB Alpha, Homo sapiens IκB Alpha (Accession No. NP_065390.1); HsIκB beta1, Homo sapiens IκB beta isoform 1 (Accession No. NP_002494.2); HsIκB beta2, Homo sapiens IκB beta isoform 2 (Accession No. NP_001230045.1); HsIκB Epsilon, Homo sapiens IκB Epsilon (Accession No. NP_004547.2); HsIκB Zeta a, Homo sapiens IκB Zeta isoform a (Accession No. NP_113607.1); HsIκB Zeta b, Homo sapiens IκB Zeta isoform b (Accession No. NP_001005474.1); HsIκB Delta, Homo sapiens IκB Delta (Accession No. NP_640332.1); TcCactus1, Tribolium castaneum Cactus isoform 1 (Accession No. NP_001157183.1) and TcCactus2, Tribolium castaneum Cactus isoform 2 (Accession No. NP_001157182.1).
Schematic representation of Arabidopsis OEP9 and amino acid alignment and phylogenetic tree of OEP9 and predicted homologues.
(a) Schematic illustration of OEP9. Numbers denote the relative position of specific amino acid residues including those that delineate the protein's single putative TMD (shaded grey, residues 36–54) and hydrophilic C-terminal sequence (CTS) (residues 55–86). Putative intrinsically disordered segments (residues 1–18 and 61–86) are indicated with stippled lines. Shown also is the deduced polypeptide sequence of OEP9's ‘NTC’ domain, including the 20 amino acid residues immediately upstream (N terminal) of the TMD, putative TMD (underlined), and CTS. Italicized and bolded amino acid residues in the CTS are those that are immunorecognized by a polyclonal antibody raised against this (synthetic) peptide sequence (see ‘Materials and Methods’ for details). (b) Multiple sequence alignment of the deduced amino acid sequences of OEP9 (At1g16000) (NCBI Accession No. NP_563987) and predicted homologues from Arabidopsis (At1g80890), Brassica (Bn), tobacco (Nt), cotton (Gh), rice (Os), maize (Zm) and moss (Pp). Identical amino acids in each protein are indicated by asterisks, and strongly similar residues are indicated by colons. Boxed are the single putative TMD in these proteins. (c) Dendogram showing the evolutionary relationship of OEP9, At1g80890 and predicted (protein) homologues from Brassica rapa (Br), Brassica napus (Bn), Lactuca saligna (Ls), Cichorium intybus (Ci), Helianthus annuus (Ha), Physcomitrella patens (Pp), Vitis vinifera (Vv), Antirrhinum majus (Am), Solanum tuberosum (St); Solanum lycoersicum (Sl), Nicotiana benthamiana (Nb), Nicotiana tabacum (Nt), Zea mays (Zm), Saccharum officinarum (So), Oryza sativa (Os), Gossypium arboreum (Ga), Gossypium hirsutum (Gh), Cyamopsis tetragonoloba (Ct), Lotus japonicus (Lj), Phaseolus coccineus (Pc), Citrus clementina (Cc), and Citrus sinensis (Cs). The branch lengths of the tree are proportional to the divergence.
Two Guns, Arizona.
Route 66 (Alignments 1926-1930s and 1930s-1938) - I-40 / US180 Exit 230.
Canyon Diablo Bridge (1915) is a beautiful arched concrete structure which may still be crossed by 4X4 vehicles traveling through the old aligment of historic Route 66 in Two Guns. (1)
Near bridge, old ruins of Cundiff Store and living quarters.
Le Canyon Diablo Bridge (1915) est une belle structure arquée en béton qui peut encore être franchie par les véhicules 4x4 circulant à travers l'ancien alignement de l'historique Route 66 dans Two Guns. (1)
Près du pont, les anciennes ruines du Cundiff Store et quartiers d'habitation.
UP Harvard Sub:Getting ready to put away the Alignment Tamper and Ballast Regulator, after a days work, headed to the spur to the former 84 Lumber in Ridgefield IL.
Workforce Alignment workshop "Building strong partnerships to support Wisconsin’s workforce need." A conversation hosted by UW Oshkosh, Department of Workforce Development, Fox Valley Tech and WAICU.
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
Frank Cenchitz checks the wheel alignment prior to welding the Pronto’s seatstay and chainstay bridges.
UMD administrators have proposed the Stadium Drive alignment (in Orange) which differs dramatically from MTA's preferred alignment along Campus Drive (in Purple).
Titan Missile Museum
Titan II ICBM Site 571-7
A theodolite would be set here to calibrate the missile IMU. Two off-site monuments would be sighted and their angles used to sight the missile via a slanted tube from the surface.
final garage wall panel alignment session using a length of rope and a large sledgehammer, a length of 2" x 4" and a set of long M8 threaded rods!
this is the front triangle on my alignment table. the table is cast iron and the accessories are by Joe Bringheli.
We have the Moon at first quarter, then Jupiter, Saturn and finally Venus forming a beautiful arc across the night sky, almost like leading lights to guide the three kings into Bethlehem.
The Moon appears to be showing a full disc in this image, as massive over-exposure of it was required to image the discs of the distant planets.
Nikon D3200, f/4, 3s, ISO-1100, 16 mm. (Sigma 10-20 lens)
The deduced peptide sequence of CmMYB1 (marked in bold) and related MYBs.a. Peptide alignment. R2 and R3 MYB DNA binding domains are shown underlined. C1 motif: LLsrGIDPX[T/S]HRX[I/L]. C2 motif: pdLNL[D/E]LXi[G/S]. C4 motif: GYDFLG[L/M]X4?7LX[Y/F][R/S]XLEMK. Zing finger motif: CX1?2CX7?12CX1?2C; b. The phylogeny of CmMYB1 and related MYBs. Bootstrap values of each branch of the derived tree are given, and the scale bar represents 0.02 substitutions per site. The genes encoding the amino acid sequences and their GenBank accession numbers are: VvMYB4a (XP_002278222), GhMYB9 (AAK19619), ZmMYB31 (NP_001105949), AtMYB4 (AAS10085), AmMYB308 (P81393), GmMYB48 (ABH02823), TaMYB1 (AAT37167), Os09g0538400 (NP_001063796), OsMYB1 (BAA23337), AtMYB32 (NP_195225), PgMYB5 (ABQ51221), HvMYB1 (P20026), HvMYB5 (CAA50221), TfMYB1 (AAS19475), ZmMYB42 (NP_001106009), AmMYB330 (P81395), AtMYB3 (NP_564176), AtMYB6 (NP_192684), EgMYB1 (CAE09058), GhMYB1 (AAN28270), TfMYB2 (AAS19476). CmMYB1 is in bold. LR: R2R3-MYB transcription factors characterized as repressors of lignin synthesis.