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The Mr Despair on the right came from a manga cover. The D&D type alignments on the left came from some loving fan.(When I find the fan's name, I'll give them the credit.)
Putting them together made a rather nice wallpaper. For the week anyway.
Domain structure, phylogeny and conservation of critical catalytic features of ciliate PIPKs.A, Domain structure of Tetrahymena PIPKs. The RING domain predicted in PIPK2b and transmembrane and SecY domains in PIPK5 have been removed (see Table S1 and Methods). Domain boundaries, e-values and further details are given in Table S1. B, Unrooted neighbor-joining tree of catalytic domains from 37 ciliate PIPKs. Bootstrap values from 5000 replicates higher than 60% are indicated near the corresponding branches. Group 1, 2, 3 and 4 PIPKs are color coded (blue, green, red and purple respectively). Circles and triangles represent Tetrahymena and Paramecium PIPKs, respectively. Bar indicates number of amino acid substitutions per site. Phylogenetic relationships of ciliate group 2 PIPK genes were less resolved with less nodes supported by high bootstrap values. In Paramecium, 4 additional group 3 PIPKs that are organized in 2 pairs of paralogs (PtPIPK3c,d and PtPIPK3e,f) and they are most related to TtPIPK3 are not shown. C, Sequence alignment of the catalytic kinase domains from ciliate PIPKs and mammalian PIPKI? and PIPKII?. The position of prominent catalytic features is indicated by arrows and arrowheads and numbered residues refer to the mouse PIPKII? structure described in reference 46. Polygons indicate residues that interact with ATP or the phosphoinositide substrate (PtdIns5P) in the crystal structure of PIPKII? and they are conserved in both type I and II PIPKs [46]. The variable inserts between the MDYSL and IID motifs present in all PIPKs have been omitted. The residues K150, D278 and D369, essential for catalytic activity, are conserved in all but 2 Tetrahymena PIPKs (highlighted in grey; see text and Figure S4 for details). The DLKGS motif in TtPIPK2c (highlighted in grey) has been reconstituted from RNA sequencing data (Table S1). The position of the KKxE/AxxxK motif in the specificity loop is indicated by a bar; further K residues that may contribute are highlighted by light blue and most ciliate PIPK1, but not PIPK2, genes confront to the consensus KK motif. Note that in all but 2 ciliate PIPKs the +2 position (E/A residues) in the specificity loop is occupied by a Glu residue as in all PtdInsP 5-kinases.
In the background of this view, the track bed serving the north portal of the Gotthard Base Tunnel can be seen above the road where the underpass takes Gotthardstrasse beneath the new railway alignment. Heading south in the foreground, SBB 11218 hauls IR2279, the 16:09 Zurich HB to Locarno.
All images on this site are exclusive property and may not be copied, downloaded, reproduced, transmitted, manipulated or used in any way without expressed written permission of the photographer. All rights reserved – Copyright Don Gatehouse
Radcliffe Camera and St Mary's church point towards the bridge to Boathouse island in Christ Church Meadows
Epic Sunset Alignment with Scripps Pier in La Jolla, CA, with the waves splashing around! This Time Lapse consists of 198 pictures taken on August 7, 2023 between 7:30 PM - 7:40 PM. This was the very first time that @adriennedragophotography and I have ever visited the Scripps Pier. Watching and photographing this Sunset Alignment at Scripps Pier together was the highlight of our visit to La Jolla, CA! Thank God that the skies were so clear yesterday, for this picture perfect view of the Sunset alignment! Sunset alignment found and planned with @planitphoto Pro. Photographed with @adriennedragophotography, @dannym87, @laceyhorner, @w.finch_photography, @johnpwise, and many others. Music is Sunset Panorama by Sacramento Horizon of @epidemicsound. 🌅🌊💦📷
The new alignment for the South Fraser Perimeter Road, detailing the path of the new Highway #17 (SFPR) and Highway #17A.
I made these this past week as part of my new head tube alignment system. On the left, an adjustible jack. On the right, a tube centering gauge. (The gauge itself I bought from Joel of Clockwork bikes; I built the base. Eventually I'll make a new gauge that's a little more to my liking.)
This garage has been like this for years now. I like this one more than my colored photograph because it's more haunting even though you can't see the blood red paint they used.
And personally I think the message is something Republicans and Democrats can believe in; regardless of political alignment, it would be nice to have these men and women home and out of harm's way..
Lupton, Arizona.
Route 66 (Alignment 1926-1985) - Grant Rd - I-40 Exit 359.
Tomahawk Indian Store.
Photo shot near the large tepee.
Note: on the flag, the red and yellow are inverted.
Photo prise près du grand tipi.
Remarque: sur le drapeau, le rouge et le jaune sont inversés.
This new section was recently opened just south of Ola. The project cost $3.6 million and improved the alignment of Hwy. 7.
I took a better picture than this one - see later on in my photostream!
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Started off by prepping the paper with gesso just to give it a bit more tooth (the paper I'm using is a bit cheap).
Wrote words with a 3B pencil all over. Started with the big red shapes, then added some green.
Decided (probably too consciously) to do a figure. Added more green around her head.
Got stuck quite a few times, then hit on lilac coloured hair. Got stuck again, decided to make her body white.
Struggled to know what to do, so eventually just stopped.
I found this a lot harder than abstract as I just didn't know where to go with it.
Still enjoyed it though!
Going over the engine alignment measurements and chart
Location:
Johnson Space Center
Morpheus Hangar
Photographer:
Joe Bibby
I never thought I'd ever get one of these, so very special thanks to Tina to generously making this happen.
Presenting the S.H. Figuarts Dragonball Super Future Trunks.
Dragonball characters often have a long and convoluted history. Even dead ones could come back to life at any given time, so death is not a certain stopping point.
I'm a bit confused on Trunks myself, or more specifically, Future Trunks. Trunks is the child of Vegeta and Bulma, and Future Trunks always seem to come from a some dystopian future. Originally introduced during the Cell Saga in Dragonball Z, I THINK that version of the character doesn't exist anymore having changed the future and all that.
This particular version comes from a future which is not ravaged by the Androids, but rather, is ravaged by Zamatsu, former Supreme Kai gone mad, and forms the basis for an entire story arc in Super resulting in Trunks gaining a new super mode known as Super Saiyan Rage, which looks like Super Saiyan 2 but with a blue and yellow aura.
But that's enough backstory - lets take a quick look at the actual figure itself.
Future Trunks comes with the figure itself which technically has two head sculpts - one normal mode, one Super Saiyan mode - with two face plates a piece. In the case of the normal head sculpt, you get a smiling and yelling, and for Super Saiyan you get yelling and gritted teeth with eyes to the right. Trunks also comes with his trusty Z Sword in broken, unbroken, and Sword of Hope modes, along with a Gallick Cannon effect and the usual assortment of weapon gripping and posing hands.
There is only one hand that can work with the Gallick Cannon effect, and there is a stand to hold the blade of the Sword of Hope for a slashing pose, though it only works for a downward slash as it is a fixed stand rather than a dynamic one.
Trunks is one of the few characters over the years that I can ever recall doing extensive fighting in civilian clothes. For the Super arc, he's dressed in.. well, Capsule Corp gear, along with his trademark boots and a fancy neckerchief. He's got blue hair now which apparently was the way it always was meant to be, as Bulma has blue hair, but was interpreted as purple for the Z series anime.
Trunks looks very accurate to his anime appearance, colours and all. Unfortunately, as his anime model only had like one second of swoleness, Trunks too remains relatively slim even in Super Saiyan Rage mode. But hey, Tamashii did a great job on that Super Saiyan hair, to the point that the hair has functional sharp points.
I guess one thing I don't quite get is why they painted the one part of the knee joint (on the torn pants side) white rather than leaving it as the grey - maybe this was something from the anime model?
You of course get the articulation of the base male Dragonball body, which includes toes, ankles, double jointed knees. hips which are permanently in the pulled down position to allow for greater range of motion, waist, mid torso, shoulders with chest collapse, bicep swivel, double jointed elbows, wrists, neck, and head. Overall, you'll get your desired pose out of the figure, but you'll probably need to find a flight stand to get the most out of it.
Paint work is beautiful, as tends to be case with the Dragonball offerings, probably because they're relatively simple. Don't get me wrong though - there's nothing wrong with simple, as executed right the result is stunning. Accent paints on the silver and gold parts are done with minimal smudging or other masking errors. All other areas are painted with a smooth, consistent texture that does not drown the underlying plastic details. The only exception is that weird grey paint app on the pants.
Finally, surprisingly the build quality of the figure is great. There are no issues with joints, material choices, limb length or alignment. Details on the figure are sharp, though there are some spots of poor sanding of plastic flashing - on the flip assembly seams seem to be better finished than the typical Figuarts figure.
As I've said before, Dragonball Figuarts are what I wish the Final Fantasy Play Arts Kai figure were. A good underlying body that combines good materials, articulation and build quality, along with QC on paint and assembly that shows despite having a monopoly on a product line, your company still cares about that sort of thing.
A flight stand and more effects parts would be nice, but that's pretty much consistent for all the Dragonball line,
Thanks again, Tina, for making this happen. I owe you a solid.
And so we come to the first of the Kent churches visited this month. Well, not quite true, as the very first church I tried to enter, St Mildred's in Preston, was locked fast as usual. Being the heritage weekend as well as ride and stride, and being on the latter list, one really hoped that the church would have made an effort, it being so remote and all.
But, they put a trestle table out, placed a rock on top of the check in sheet to stop it blowing away, and left the church for the day, despite arrangements having been made by another church the day before for it to be open.
This really is not good enough.
Anyway, St Mildred's was the first of three that were locked, but I managed to gain entry to seven previously closed churches to me. So, on the whole, I was pleased.
St Nicholas is a large and imposing church, with a huge churchyard, showing that it is one of the larger and better populated parishes in the area of east Kent.
There was a warden sitting at the table in the large doorway, and after a warm welcome we entered inside.
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The present parish of Ash, more than 7,000 acres in extent and one of the largest in Kent, was once only a part of the great manor of Wingham. Originally a royal manor, Wingham was given by King Athelstan of Kent to the See of Canterbury about 850 : it covered the present parishes of Ash, Goodnestone, Nonington, Wingham and parts of Staple and Womenswold.
In a list of churches probably made in 1071, in which 'Aesce' is said to belong to Wingham, mention is also made of an apparently more important church 'de Raette', as well as one at 'Fleota' belonging to the manor of Folkestone. If, as seems likely, 'de Raette' refers to Richborough, this is the only record of that church; but the chapel of Fleet, actually within the 3rd century Roman walls of Richborough Castle, continued in use until the 16th century. Leland in the time of Henry VIII wrote that 'withyn the castel is a lytle paroche Chirch of S. Augustine'.
It was believed that when St. Augustine first stepped ashore in England in 597 the impression of his foot was miraculously left upon a stone. This relic was afterwards kept in this chapel dedicated to him, and pilgrims flocked there upon the anniversary of the landing to pray and to recover their health. Excavations have uncovered the ground plan of the chapel, and confirm that it was pre-Norman in origin. Excavations in the northwest comer of the Roman fort have also, revealed the foundations and font of an even earlier church of c.400, one of the earliest Christian structures known in Britain.
By the 13th century there was another chapel in the northwest of the parish, at Overland, where complaint was made in 1294 that 'there used to be a baptistery and seven years ago it was taken away and is at Esse'. Edward Hasted, writing about 1790, said that the chapel had been 'for some time in ruins ... having been desecrated about the beginning of this century'. Its exact site has now been lost : some carved stonework which may come from it is at Knell; a few more pieces are in the church.
In 1282 Ash became a separate parish. In the deed founding a College of Canons at Wingham and dividing that parish into four, Archbishop Peckham explained, 'We have turned our eyes to the church of Wingham as it were to a fruitful vineyard..... which cannot be easily cultivated by the labours of one husbandman... from the great extent of the parish as well as its numerous population'. He assigned to Wingham parish church the chapel of Overland; to Ash he gave the chapel of Fleet. It was the duty of the canons of Wingham College, to whom the tithes of Ash were paid, to provide a vicar. In 1535 the parishioners of Ash complained: 'There has always been a vicar here to serve the cure till for the last 22 years the said Canons have usurped the vicarage to their own use ... within a quarter of a year we have had seven curates, which has caused much strife as we are 500 residents.'
In 1547 Wingham College was suppressed by Henry VIII, and its possessions forfeited to the Crown. In 1549, "the late chapel called Richborough Chapel in Ash Parish with its burial ground, buildings, lead, glass, iron, stones and tiles except the bells and leaden roof", and "the late Chapel of Overland in Ash parish next Sandwich in width 22 feet in length 34 feet, with its burial ground of half a rod, buildings, etc.", were both sold to William Hyde and Hugh Cartwright.
The right of presentation to the benefice of Ash was granted by Queen Mary to the Archbishop in 1558, and three years later Queen Elizabeth I gave the rectory - the right to the great tithes - to the See of Canterbury. The Archbishop is still the patron of the living today.
In the 19th century the need again arose for chapels in the more distant parts of the parish. In 1842 Holy Trinity Church at Ware was built, and Westmarsh was formed into a separate ecclesiastical parish in 1849. The corrugated-iron mission room of St. Augustine's, Richborough, was opened in 1888. It was followed in 1892 by a similar room at Goldstone, rebuilt in 1904. But by the 1960s the motor car had made these separate buildings less necessary. In 1967 the parish of Westmarsh was re-united with Ash; St. Augustine's, Richborough, was closed in 1969, and Holy Trinity Church in 1970.
An unusual feature of the church is the south chancel, whose axis is out of alignment with the nave. It was once supposed that this architectural oddity represented the inclined head of Christ on the Cross, but a structural fault caused by rebuilding and restoration is a more likely explanation.
(Bygone Kent, 1985, Michael David Mirams.)
In recent years the Parish of Ash with Westmarsh has been linked to the parish churches at Goodnestone and Chillenden through a united benefice. Further pastoral reorganisation in the East Bridge Deanery means that a new canonry benefice is to be formed of the parishes of Ash, Chillenden, Elmstone, Goodnestone, Preston and Wingham. This is expected to be undertaken between 2012 and 2014.
Previous: Adjusting the gamma curve
Building the frames for Hugin
Once I have reviewed all frames and added the build rules to my Makefule, I add this rule:
AonachDubh.pto: aonach_dubh-f1.tiff aonach_dubh-f2.tiff aonach_dubh-f3.tiff aonach_dubh-f4.tiff aonach_dubh-f5.tiff aonach_dubh-f6.tiff
echo "create AonachDubh.pto with Hugin"
In the absence AonachDubh.pto, this will work as a dummy target, causing make to generate the TIFF versions of all frames. Once I have created the panorama file AonachDubh.pto with Hugin, an as long as all frames are present, this rule will have no effect.
Automatic alginment
Adding the frame files to a new panorama in Hugin using its Assistant interface will make it assign the control points and attempt to align the images in one go. With a good enough set of frames, such alignment will normally run without problems, but with this odd set I've got the first result was a bit disappointing.
The mess shown here is caused by a combination of factors, including the lack of overlap between some frames, bogus control points found in the farmes that did not overlap, too few control points between well-overlapping frames, too many control points concentrated in very small areas, and perhaps other problems I am not aware of. The solution in this case was to delete the automatically assigned control points and place them manually, aiming for the best spread.
But before we do that, let's take a look at a few bad cases of control point positioning.
Zachry Construction's foreman checks the alignment and placement of the first concrete girder for the new Radio Road Bridge over I-40 East.
The screw located just behind the frameline mask can only be fully visible when the lens is focused at infinity. The screw moves the rangefinder patch focusing lens (A) forward or backward for horizontal alignment. It is at infinity that the horizontal alignment can be most accurately done.
The rest of the labellings are to explain which parts serves what purpose in the rangefinder.
Sequence analysis of SsCBF1.(A) Amino acid sequence alignment between SsCBF1 and other known CBF1s. The alignment was performed using ClustalX 2.0 and DNAMAN software. Black background indicated conserved residues among all the proteins selected. The AP2 DNA-binding domain and other signature motifs were indicated by solid lines. (B) Phylogenetic relationships between SsCBF1 and other CBFs from various species. The phylogenetic tree was generated by the neighbor-joining method using MEGA 5.0. Organisms were abbreviated as follows: St, Solanum tuberosum; Sc, Solanum commersonii; Sl, Solanum lycopersicum; Ss, Solanum lycopersicoides; Ca, Capsicum annuum; At, Arabidopsis thaliana. GenBank accession numbers of the CBFs are listed as follows: AtCBF1 (AEE85066), AtCBF2 (AEE85064), AtCBF3 (AEE85065), AtCBF4 (ABV27186), SsCBF1 (ACY79412), SlCBF1 (AAS77820), SlCBF2 (AAS77821), SlCBF3 (AAS77819), ScCBF1 (ACB45093), ScCBF2 (ACB45094), ScCBF3 (ACB45092), ScCBF4 (ACB45084), StCBF1 (ABI74671), StCBF2 (ABI94367), StCBF3 (ACB45095), StCBF4 (ACB45083), StCBF5 (ACB45082), CaCBF1 (AAZ22480), CaCBF3 (ADM73296).
Planetarium V2
Amazing true action planetarium
Posted by Second Life Resident Torley Linden. Visit France Creation.
I had the pleasure of viewing the planetary alignment this week shortly after the sun set into Lake Michigan. In this photo I captured the Moon, three planets, and a few deep sky attractions using a wide angle 10mm lens . The reflection of Venus in the lake is also prominent.
Location: Sawyer, Michigan
Date: March 28, 2023
Sequence alignment of TaNAC67 and NACs in various plant species.A. Amino acid alignment of TaNAC67 and other NAC family members from selected plant species. The numbers on the left indicate amino acid position. Shared amino acid residues are in black background. Gaps, indicated by dashed lines are introduced for optimal alignment. The region underlined indicates the conserved NAC-domain. ?, conserved amino acid motif (AA sequences in red rectangles). Alignments were performed using the Megalign program of DNAStar. B. Phylogenetic tree of TaNAC67 and NAC members from other plant species. Abbreviations: At, Arabidopsis thaliana; Bd, Brachypodium distachyon; Eg, Elaeis guineensis; Gm, Glycine max; Hv, Hordeum vulgare; Os, Oryza sativa; Sb, Sorghum bicolor; Sl, Solanum lycopersicum; Vv, Vitis vinifera; Zm, Zea mays. The phylogenetic tree was constructed with the PHYLIP 3.69 package, and the bootstrap values are in percent.