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Just received it today. It was too expensive to ship the one made by Joel (Clockwork) from the US, so I found somebody who could CNC mill it for me. It also allowed me to customize it for my height gauge.
The design is very, very much inspired by this one ! :
www.flickr.com/photos/duncancycles/2578528943/in/set-7215...
Noticed these misalignment issues when trying to assemble the line of pier and waterline (I thought it was me who was the problem!). These puzzles all have 3 vertical seams: between pieces 13/14, 25/26, and 37/38. Here you can see the middle and right-hand seam, and how the pieces are off alignment (but only in some areas, it looks like!). I have come to expect small alignment issues in puzzles, but it's pretty bad to see this in just a 2000 piece.
This hasn't completely ruined my puzzle experience, but I can already hear the complaints from fellow collectors. Making high-art puzzles is going to attract a picky audience. Probably a big reason why former art puzzle manufacturer Ricordi folded had to do with complaints from customers over quality issues. I remember reading many negative comments about Ricordi on Amazon, the German puzzle forum and from other sources, and when I finally got around to building some Ricordis last year I was surprised to find the quality decent if not top-notch. Grafika will need to do a better job than this to keep its customers - though on the other hand, art puzzle collectors are so desperate for new images that they may live with these issues. Wouldn't it be nice if we could have the best of both worlds: well-chosen art designs combined with exquisite manufacturing.
Summary of cpcB.(A?B) cpcB mRNA levels during the life cycle of A. nidulans (A) and A. fumigatus (B). Conidia (asexual spores) are indicated as C. The numbers indicate the time (hours) after incubation in liquid MMG (Vegetative), and on solid MMG under conditions inducing conidiation (Asexual) or sexual development (Sexual). The relative band intensities (mean?SD) of Northern blot were determined by densitometric scanning of mRNA bands using ImageJ and each band was normalized by the amount of 18S rRNA (cpcB/18S rRNA) in the relevant lane. Ethidium bromide staining of ribosomal RNAs was used as a loading control. (C) Alignment of A. nidulans (Ani) CpcB, A. fumigatus (Afu) CpcB, A. flavus (Afl) CpcB with Gib2 of C. neoformans var. grubii (Cgr; accession: AY907679) and C. neoformans var. neoformans (Cne; accession: AY907680). ClustalW (align.genome.jp/) and BoxShade 3.21 (www.ch.embnet.org/software/BOX_form.html) were used for the alignment and presentation. (D) A phylogenetic tree of CpcB-like proteins identified in various fungal species including A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, A. terreus (A?=?Aspergillus), C. neoformans, Neurospora crassa, Penicillium chrysogenum, Penicillium marneffei, Schizophyllum commune, Schizosaccharomyces japonicus and Talaromyces stipitatus. The putative CpcB proteins were retrieved from NCBI BlastX (blast.ncbi.nlm.nih.gov/Blast.cgi) using A. nidulans CpcB. A phylogenetic tree of 14 putative CpcB homologues was generated by the TreeTop software (genebee.msu.su/services/phtree_reduced.html) using the alignment data from ClustalW. The phylogenetic tree is constructed based on the matrix of pairwise distances between sequences. Numbers indicate the computed distances given the residue substitution weights from the alignment data [59].
Megalithic tomb (Passage grave) Newgrange, Co Meath, Ireland - 2500BC. Dated to around 3200 BC, Newgrange is 1,000 older than Stonehenge and centuries older than the great pyramids of Egypt.
At the winter solstice, the shortest day of the year, the light of rising sun penetrates the tomb through the roofbox over the entrance, gradually illuminating the passage for a distance of 62feet to the three chambers at the end.
(I believe it didn't happen to day - too dark, damp and grey!). Photo taken several years ago.
Name: Frizzak
Title: Toa of Ice
Alignment: Lawful Good
Location: Ko-Magna
Weapons: Katana of Frost, Wakazashi of Air, Fists
Lore: A relative rarity on Strata Magna, Frizzak is one of the few surviving original Toa of the island. Originally lost after a rescue mission gone wrong resulted in him being frozen in crystal by a Bohrok elite, he was rescued by the Matoran of Ko-Magna 15 years later as the village began to expand and reclaim land once owned by the Bohrok. As a Toa however, the time jump meant little to him – his morals had not changed. He once again took up his position as Guardian of Ko-Magna, guarding the Thousand Year Gate that stands at its entrance. Whilst easily annoyed, he is a relatively kind Toa, at least in comparison to others that master the element of Ice. Whilst capable of manipulating Ice at his will, Frizzak prefers swordsmanship over his elemental powers, being trained in a variety of two-sword techniques, as well as excellent hand to hand combat. He forged his Katana of Frost upon first washing up on the island, and obtained his Wakazashi of Air from an old Toa of Air, who fought with him at the First Bohrok War. With the combination of Frost and Air, he utilises stun effects on his enemies to unleash heavy blows with speed and grace.
Pickup alignment of a Lyra Helikon mounted on Alphason HR100S (and Oracle Delphi) using "Schön"-template.
this is a panorama made from 8 pictures.
It was taken on May, the 1rst, at 3:00 AM GMT.
At this moment, Venus and Jupiter were in conjunction. The milky-way core was still visible.
Location : La Seyne sur Mer, near Toulon, France.
Setup : Nikon Z6 II + Nikkor 24/70 @ 24 mm -f/4 - ISO 1600 - 20 s
The magnets do an amazing job keeping the TouchFire aligned.
This photo shows something else: silicone rubber grabs dust and hair. You can run it under water to clean that stuff off.
Read the full review here:
www.suntimes.com/technology/ihnatko/13715183-452/touchfir...
I am measuring any twist between the seattube and the headtube. .0035 inches is pretty much as good as it gets.
Ball technicians use specialized equipment to build the GPIM satellite so that the space vehicle instruments and thrusters align perfectly with the payload interface. Image credit: Ball Aerospace & Technologies Corp.
Sequence alignment and phylogenetic analysis of SKD1 proteins from Zea Mays and other species.A An alignment is shown for the deduced amino acid sequence of SKD1s from Zea Mays (Zm), Arabidopsis thaliana (At), Lycopersicon esculentum (Le), Oryza sativa (Os), Brachypodium distachyon (Bd), Hordeum vulgare (Hv) and Saccharomyces cerevisiae (Sc). The numbers on the left indicate the amino acid position. Identical residues in all these proteins are shown in a black background. Dashes indicated gaps introduced for optimal alignment. The putative MIT and AAA domains are underlined with a thick red line and a thick green line, respectively. B Phylogenetic tree based on SKD1 protein sequences from yeast, plants, and animals. The bootstrap values shown were calculated based on 500 replications. The tree was constructed using the neighbor-joining method. Z.mays, AY105155; A.thaliana, At2g27600; L.esculentum, AK324437; O.sativa, AF499028; B.distachyon, LOC100837561; H.vulgare, AK359160; D.melanogaster, NP_573258; G.gallus, AJ720732; H.sapiens, AF038960; M.musculus, NP_033216; S.cerevisiae, NP_015499.
Somewhere in the Stockholm archipelago. Taken from the ferry between Nynäshamn and Nåttarö (also spelled Nåtarö, sometimes).
Alignment, of place and time, is...hard to come by, hard to plan, hard to live by. But when it comes around, sets you back in that delusional state of mind that pursues dreams.
This seemed like a good idea at the time, but one of the screw heads for the machine heads sheared off. I was not happy.
Sequence and expression analysis of GsWRKY20.(a) Amino acid sequence alignment of WRKY domains among GsWRKY20 and other type â…¢ WRKY TFs, Sequences were aligned using ClustalW, and gaps were introduced to maximize alignment, filled triangle marks the cystine and histidine in the C2HC-type zinc finger domain.(b) The phylogenetic tree of the WRKY TFs. The phylogenetic tree was constructed using MEGA 4.1. Total 24 WRKY proteins from Oryza, Arabidopsis, Gossypium, Medicago, Triticum aestivum, Glycine max, Thlaspi caerulescens and Solanum were selected to construct the phylogenetic tree.(c) Tissue-specific expression analysis of GsWRKY20 by real-time quantitative PCR (qRT-PCR). Tissues included trifoliate leaf (TL), root (RO), stem tip (ST), flower bud (FB), and pod (PD). Expression of GAPDH was used as an internal control. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.(d) qRT-PCR analysis of GsWRKY20 diurnal expression under SD and LD. Trifoliate leaves were sampled every 4 h at 21 DAE. White and black bars at the top represent light and dark phases, respectively. Relative transcript levels were analyzed by qRT-PCR and normalized by GAPDH. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
Radcliffe Camera and St Mary's church point towards the bridge to Boathouse island in Christ Church Meadows
Sequence, structure, and phylogenetic analyses of various MtnDs.(A) Multiple sequence alignment of the MtnD family. Magenta bars show highly conserved cupin motifs I and II, magenta and green triangles show amino acid residues involved in metal binding and catalysis, respectively. (B) Predicted tertiary structure of T. thermophila MtnD. The structure of MtnD was modeled on that of M. musculus MtnD (PDB ID: 1VR3) using Swiss-model. Orange sphere represents a nickel atom. His307, His309, Glu313 and His352 (shown in magenta) form the metal binding site. Phe303, Arg315, and Phe 354 (shown in green) are essential for dioxygenation. (C) Phylogenetic tree based on primary sequences of the MtnD family. Alignments were created with ClustalW and displayed using Treeview. Scale bar indicates a difference of 0.1 substitutions per site. Names and gene accession numbers are as follows: A. gambiae (XP_315627), A. aeolicus (NP_214354), A. thaliana (NP_567443), A. nidulans (XP_664180), B. subtilis (NP_389245), C. elegans (NP_510072), C. variabilis (EFN58452), C. neoformans (XP_572521), D. rerio (NP_955962), D. discoideum (XP_635174), D. melanogaster (NP_001097577), E. cloacae 13047 (YP_003613573), G. kaustophilus (YP_146809), H. sapiens (NP_060739), H. magnipapillata (XP_002165167), K. oxytoca (AAD11793), M. aeruginosa (A8YMJ4), M. brevicollis MX1 (XP_001750499), M. musculus (NP_598813), N. crassa (XP_956660), O. sativa (AAC05511), P. aeruginosa (NP_250375), S. cerevisiae (NP_013722), S. pombe (NP_596475), S. purpuratus (XP_789320), S. elongatus (YP_171626), V. vinifera (CBI26309), V. carteri (XP_002955803), X. tropicalis (NP_001004933). Proteins described in (A) are underscored.