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Amino acid sequence alignment and phylogenetic analysis of CsVDE and homologous proteins.(A) Schematic description of CsVDE domains. (B) Alignment of the deduced amino acid sequences of VDE in different plants. Red lines indicate the VDE Cys-rich domain; Gray lines indicate the Lipocalins domain; Blue lines indicate the Glu-rich domain. The important residues for pH switch are marked with black stars and the putative active site residues with black squares reference from Arnoux et al. (2009) [11]. Arabidopsis thaliana VDE (accession No. AEE28305), Zingiber officinale VDE (accession No. AAX59986), Lactuca sativa VDE (accession No. AAC49373), Nicotiana tabacum VDE (accession No. AAC50031), Coffea arabica VDE (accession No. ABB70816) sequences are shown. Black indicates 100% homology of the amino acid. Red indicates 75% homology of the amino acid. Green indicates 50% homology of the amino acid. (C) Phylogenetic analyses of selected VDEs. Phylogenetic studies were carried out using MrBayes3.1.2 and viewed with the TreeView package. All the trees were obtained with 200,000 generations for the chains, a sample frequency of a 10, and a burn in of 5,000 (ngen = 200000; Samplefreq = 10; burnin = 5,000). Camellia sinensis VDE (accession No. AAL67858), Vitis vinifera VDE (accession No. XP_002267152), Osterococcus tauri VDE (accession No. XP_003083515), Ostreococcus lucimarinus VDE (accession No. XP_001421704), Micromonas sp. VDE (accession No. XP_002503106), Micromonas pusilla VDE (accession No. XP_003061123), Ectocarpus siliculosus VDE (accession No. CBJ26509), Phaeodactylum tricornutum VDE (accession No. XP_002178643), Oryza sativa Japonica Group VDE (accession No. AAL83562), Zea mays VDE(accession No. NP_001147756), Solanum lycopersicum VDE (accession No. ACM92036).
You may see layer alignment issues on prints with flat surfaces; notice how the print on the left exhibits the problem very well but the contour of the print on the right masks the problem to a certain degree. This can be caused by a variety of issues, but here are some of the things you can do to get more consistency:
1. Slow down if using non-Marlin firmware. Print your perimeter at 20-25 mm/sec
2. Tighten your belts.
3. Make sure any springs on your build platform are very tight and that the top plate can't wobble around independent of the bottom plate.
4. Upgrade your Z-coupling to a better one, such as www.thingiverse.com/thing:11220
5. Replace any non-straight threaded rod used in the Z-axis with perfectly straight ones.
6. Unless they were printed perfectly, replace your timing pulleys with manufactured ones, such as sdp-si.com/eStore/PartDetail.asp?Opener=Group&PartID=...
Sequence analysis of eukaryotic and prokaryotic SLC1 transporters.(A) Sequence alignment of TMD8 region from human, insect, bacterial, and archael transporters. CuqDCT, CuqEAAT, AeaDCT, and AeaEAAT represent the EAAT and DCT orthologs from Culex quinquefasciatus and Aedes aegypti, respectively. AngEAAT1 and AngEAAT2 represent the transporters from Anopheles gambiae. Drosophila EAAT1/2 are from Drosophila melanogaster, Gltph from Pyrococcus horikoshii, and DctA is from Bacillus subtilis. Highlighted TMD8 residues involved in substrate binding are labeled above using human EAAT3 numbering. R447 and N451 are conserved in all EAAT-like orthologs, while D444 and T448 are changed to asparagine and alanine, respectively in the CuqDCT and AeaDCT mosquito transporters. Sequence alignments were performed with ClustalX2 and Jalview. (B) The phylogenetic relationship of the EAAT and DCT proteins from Culex quinquefasciatus and Aedes aegypti mosquito species together with the two EAATs from Drosophila (ClustalX2 based alignment).
A group of core networking volunteers and friends from past events (like What the Hack 2005, Hacking at Random 2009, Chaos Communication Camp 2011, Observe. Hack. Make 2013) needed to make alterations to a few kilometers of fiber optic cable to be ready for upcoming events such as Chaos Communication Camp 2015, Campzone 2015 and SHA2017.
This turned into a fusion splicing (fiber splicing) workshop.
Finding chance alignments of random points - in this case, pubs in Scotland.
A pub ley line has
- at least 8 pubs in a line
- all pubs are within 5 meters of the line.
- the whole line must be at least 10 Km long, to remove short lines of pubs in straight streets... so Rose Street doesn't count :-)
This is only a 10% sample from 2.5 million lines between pubs; there will be other alignments. From 200,000 inter-pub lines, I found 4 ley lines. In case you're wondering, the lines end in Glencoe, Ayr, Perth and Stranraer.
Using data copyright OpenStreetMap and its contributors. Used osm2pgsql, PostgreSQL with postGIS, pgsql2shp and QGIS.
From St Paul's, the Midland Metro follows a new alignment that skirts Snow Hill station and enters the street.
I had the pleasure of viewing the planetary alignment this week shortly after the sun set into Lake Michigan. In this photo I captured the Moon, three planets, and a few deep sky attractions using a wide angle 10mm lens . The reflection of Venus in the lake is also prominent.
Location: Sawyer, Michigan
Date: March 28, 2023
I decided to make my own dropout alignment tools. The Park ones have the annoying feature of moving around in the dropout while you're trying to tighten them down. The Stein ones solve this by using quick-release clamps, but then require you to adjust the spacing with a difficult-to-use collar instead of a screw thread (and they cost 3x as much). So here's the best of both worlds: quick-release clamps, long arms, and a nice tight screw thread adjustment.
each section is only as wide as the hoop. there were 5 sections i had to line up in descending order.
i had to pluck out the running stitch 3 times on that eagle, scoot the fabric left, right... no, no, back to the left... before it FINALLY centered itself between the triforce's...uh... legs.
Sequence alignment and phylogenetic tree of LHP1 and HP1 homologues.
(A) Segment of the alignment that contains the chromodomain. The arrow heads highlight the aromatic cage residues that form the binding cavity for histone methyl groups. (B) Phylogenetic tree of 15 LHP1 and HP1 homologs (Arabidopsis thaliana: At_LHP1 GI:15625407. Populus trichocarpa: Pt_LHP1A, estExt_Genewise1_v1.C_LG_XIX1329; Pt_LHP1B, eugene3.00130688. Oryza sativa: Os_LHP1, GI:110810411. Zea mays: Zm_LHP1 GI:22135459. Physcomitrella patens: Pp_LHP1, jgi|Phypa1_1|169812|estExt_fgenesh1_pg.C_2200058. Selaginella moellendorffii: Sm_LHP1, jgi|Selmo1|407083|fgenesh2_pg.C_scaffold_6000334. Homo sapiens: Hs_HP1alpha, GI:6912292; Hs_HP1beta, GI:48428808; Hs_HP1gamma, GI:5732187. Drosophila melanogaster: Dm_HP1a, GI:17136528; Dm_HP1b, GI:24640713. Caenorhabditis elegans: Ce_HP1-1, GI:17568757; Ce_HP1-2, GI:71987888. Schizosaccharomyces pombe: Sp_SWI6, GI:510930.). The evolutionary history was inferred using the Maximum Parsimony method; the most parsimonious tree with length = 2089 is shown. Support for each node, assessed with bootstrap analysis (1000 replicates) is given when higher than 60%. Note that the tree is displayed as circular cladogram with all branches of the same length.
This IG3OA is seen sitting shy of the Amtrak Sacramento Station while a trespasser on the I Street Bridge is dealt with.
In the new depot alignment, this train in the 21st century is taking close to the same path its ancestors did up to 50 years ago through Sacramento, CA.
©2002-2013 FranksRails.com Photography
Bulbs (in both senses), knights and pelvic alignment sums it up. A 'hip' picture, I hope you'll agree.
Just received it today. It was too expensive to ship the one made by Joel (Clockwork) from the US, so I found somebody who could CNC mill it for me. It also allowed me to customize it for my height gauge.
The design is very, very much inspired by this one ! :
www.flickr.com/photos/duncancycles/2578528943/in/set-7215...
"The Alignment Guy", the early years: Employee with a bad attitude by day, would be shop owner by night.
Sorry for the tiny photo. I can't find a larger copy, or the memory card the original is on.
Noticed these misalignment issues when trying to assemble the line of pier and waterline (I thought it was me who was the problem!). These puzzles all have 3 vertical seams: between pieces 13/14, 25/26, and 37/38. Here you can see the middle and right-hand seam, and how the pieces are off alignment (but only in some areas, it looks like!). I have come to expect small alignment issues in puzzles, but it's pretty bad to see this in just a 2000 piece.
This hasn't completely ruined my puzzle experience, but I can already hear the complaints from fellow collectors. Making high-art puzzles is going to attract a picky audience. Probably a big reason why former art puzzle manufacturer Ricordi folded had to do with complaints from customers over quality issues. I remember reading many negative comments about Ricordi on Amazon, the German puzzle forum and from other sources, and when I finally got around to building some Ricordis last year I was surprised to find the quality decent if not top-notch. Grafika will need to do a better job than this to keep its customers - though on the other hand, art puzzle collectors are so desperate for new images that they may live with these issues. Wouldn't it be nice if we could have the best of both worlds: well-chosen art designs combined with exquisite manufacturing.
Summary of cpcB.(A?B) cpcB mRNA levels during the life cycle of A. nidulans (A) and A. fumigatus (B). Conidia (asexual spores) are indicated as C. The numbers indicate the time (hours) after incubation in liquid MMG (Vegetative), and on solid MMG under conditions inducing conidiation (Asexual) or sexual development (Sexual). The relative band intensities (mean?SD) of Northern blot were determined by densitometric scanning of mRNA bands using ImageJ and each band was normalized by the amount of 18S rRNA (cpcB/18S rRNA) in the relevant lane. Ethidium bromide staining of ribosomal RNAs was used as a loading control. (C) Alignment of A. nidulans (Ani) CpcB, A. fumigatus (Afu) CpcB, A. flavus (Afl) CpcB with Gib2 of C. neoformans var. grubii (Cgr; accession: AY907679) and C. neoformans var. neoformans (Cne; accession: AY907680). ClustalW (align.genome.jp/) and BoxShade 3.21 (www.ch.embnet.org/software/BOX_form.html) were used for the alignment and presentation. (D) A phylogenetic tree of CpcB-like proteins identified in various fungal species including A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, A. terreus (A?=?Aspergillus), C. neoformans, Neurospora crassa, Penicillium chrysogenum, Penicillium marneffei, Schizophyllum commune, Schizosaccharomyces japonicus and Talaromyces stipitatus. The putative CpcB proteins were retrieved from NCBI BlastX (blast.ncbi.nlm.nih.gov/Blast.cgi) using A. nidulans CpcB. A phylogenetic tree of 14 putative CpcB homologues was generated by the TreeTop software (genebee.msu.su/services/phtree_reduced.html) using the alignment data from ClustalW. The phylogenetic tree is constructed based on the matrix of pairwise distances between sequences. Numbers indicate the computed distances given the residue substitution weights from the alignment data [59].
Megalithic tomb (Passage grave) Newgrange, Co Meath, Ireland - 2500BC. Dated to around 3200 BC, Newgrange is 1,000 older than Stonehenge and centuries older than the great pyramids of Egypt.
At the winter solstice, the shortest day of the year, the light of rising sun penetrates the tomb through the roofbox over the entrance, gradually illuminating the passage for a distance of 62feet to the three chambers at the end.
(I believe it didn't happen to day - too dark, damp and grey!). Photo taken several years ago.