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This image shows two neurons of the amygdala, a brain region involved in emotional learning and processing. Before imaging the neurons, we recorded their electric activity and filled them with a small molecule that allows imaging and reconstruction of the neurons morphology. We are interested in how the brain learns to assign a predictive value to sensory information. Since the amygdala is known to be involved in reward and fear learning, we hypothesized that after fear or reward associative learning, the morphology of the neuron will be modified. On this type of image we can quantify the number, the length and the ramification rate of the dendrites and the axon as well as the size of the cell body of the neurons.
Neuromics Hn2 cells grown in culture for 4 days and stained with our chicken polyclonal to MAP2, a marker of neurons. Differentiating cells show strong cytoplasmic staining for MAP2. . Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these culture
Its probably just me but I couldn't help but think this sea weed, which was everywhere in the rockpools resembled axons and nodes of ranvier.
CREDITS: Migliorini Elisa, Grenci Gianluca, Marco Lazzarino/IOM-CNR Laboratorio TASC e Centro di Biomedicina Molecolare (CBM) Area Science Park, Basovizza, Trieste.
Son de esos dÃas en los que todo lo dudas... expresar el anÃmo es algo complicado. aqui os dejo una muestra de ideas saturadas. Tony