I have had an idea to take photographs of the same diatom slide (a small arrangement) using various contrast techniques for comparison.

The photos are labelled A-K. I know what each was taken with. Techniques (in no particular order!) include;

dark-ground

phase contrast

Hoffman Modulation Contrast (HMC) (DIY)

DIC

oblique

bright-field

relief contrast (Leica, I think designed for inverted)

variable amplitude contrast (VAC)

All were taken with a x10 Plan Apo objective, apart from a couple eg. Phase which is a x10 Plan Fluor

All are stacked images of 7-11 individual images, and processing is minimal, and broadly the same.

Pollen again

Navicula

Yeast at 1000x oil immersion

Sony dsc

Organic lampwork bead

Flower from Leptospermum species.

UNMC Electron Microscopy Laboratory

Found by the Longshaw Fungi and Lichen Survey Team on June 23.

H and HB tips. They look pretty much the same to me.

Blåaktiga ascitoppar i reaktion med Melzers reagens.

Plymouth Electron Microscopy Centre

www.plymouth.ac.uk/emc

Plymouth Electron Microscopy Centre

www.plymouth.ac.uk/emc

x4 objective, 2 LED lights, copier paper used for diffusion. Dead specimen.

The view from Merritt College, Oakland, CA

Plymouth Electron Microscopy Centre

www.plymouth.ac.uk/emc

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Investigation of Local Medicinal Remedies

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Plymouth Electron Microscopy Centre

www.plymouth.ac.uk/emc

eye x200

Because I haven't had a chance lately to sit down and take some new microscopy photos, I've uploaded a few scraps here from old sessions.