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Microscopy x1000
Trypanosoma brucei est une espèce de protiste parasite de l'ordre des Trypanosomatida. L'espèce provoque des maladies, des trypanosomiases chez l'homme et les animaux en Afrique. Il est transmis par des morsures de mouche tsé-tsé. Il existe 3 sous espèces de T.brucei: T.b.brucei, T.b.gambiense et T.b.rhodesiense.
Le trypanosome responsable de la maladie du sommeil est Trypanosoma gambiense. C'est un des plus étudiés. Son cycle de vie nécessite deux hôtes pour se développer et se reproduire. Outre l'homme, il a besoin de la mouche tsé-tsé (Glossina palpalis) qui est son vecteur.
Le trypanosome vit dans le sang, la moelle osseuse et le liquide céphalo-rachidien ou LCR de l'homme. On le trouve aussi chez les antilopes et les bovidés. Sa taille est de 20 µ ou micromètres de long sur 2 à 3 µ ou micromètres de large, ils sont entièrement mobiles.
Lorsque la mouche tsé-tsé pique une personne infestée, les trypanosomes passent dans l'intestin de la mouche et ils vont s'y multiplier. Ensuite ils vont passer dans le proventricule puis vont remonter dans les glandes salivaires où la multiplication se poursuit (asexuée).
Les parasites vont passer 18 à 25 jours dans la mouche puis seront transmis à l'homme par piqûre. La période d'invasion du parasite se fait après incubation de 10 à 15 jours. La propagation se fait aussi dans la lymphe ce qui provoque les fièvres. Il y a hypertrophie du foie (hépatomégalie) et de la rate (splénomégalie).
Quelques semaines après, ils envahissent les méninges et le liquide céphalo-rachidien provoquant tout d'abord des céphalées puis des troubles moteurs et des troubles réflexes puis des troubles psychologiques. La maladie est mortelle en quelques mois.
Trypanosoma brucei is a species of parasitic kinetoplastid belonging to the genus Trypanosoma. The parasite is the cause of a vector-borne disease of vertebrate animals, including humans, carried by genera of tsetse fly in sub-Saharan Africa. In humans T. brucei causes African trypanosomiasis, or sleeping sickness. In animals it causes Animal trypanosomiasis, also called nagana in cattle and horses. T. brucei has traditionally been grouped into three subspecies: T. b. brucei, T. b. gambiense and T. b. rhodesiense. The first is a parasite of non-human vertebrates, while the latter two are the known parasites of humans. Only rarely can the T. b. brucei infect a human.
T. brucei is transmitted between mammal hosts by an insect vector belonging to different species of tsetse fly, including Glossina palpalis in humans. Transmission occurs by biting during the insect's blood meal. The parasites undergo complex morphological changes as they move between insect and mammal over the course of their life cycle. The mammalian bloodstream forms are notable for their cell surface proteins, variant surface glycoproteins, which undergo remarkable antigenic variation, enabling persistent evasion of host adaptive immunity leading to chronic infection. T. brucei is one of only a few pathogens known to cross the blood brain barrier. There is an urgent need for the development of new drug therapies, as current treatments can prove fatal to the patient
Micro-photography set up.
Compound microscope with built in digital camera and DSLR attachment. LED lighting for photographing non opaque objects under high magnification.
Stereo microscope with similar features. both microscopes linked to laptop.
I continue here with my microscopic dissection of the Alyssum flowering plant. I believe that the small yellow structure shown here is the Stigma end of the Pistal. My image was created using a Nikon light microscope. Several images focused at different planes were photostacked together using Helicon Focus, with additional post processing work in Lightroom and Photoshop. The polar width of the small granules (pollen?) in this image is approximately 20 microns. You can also see many of the individual yellowisn retangular shaped cells making up the Pistal.
Further information
"The pistil is the name given to the entire female reproductive system contained in a flower. It is composed of the stigma, style and ovary, which houses the ovules that eventually become seeds. The pistil contains all the structures required to trap and grow compatible pollen tubes until they are capable of successfully fertilizing ovules.
The stigma is the sticky, swollen structure at the top of the pistil. Its job is to capture and hold onto pollen grains. As they cling to the stigma, these dry reproductive structures are rehydrated; the stigma then sends hormonal signals to the pollen grains that tell them to elongate into the pistil.
The style is a long, hollow tube that holds the stigma far enough away from the ovary to be able to catch pollen grains as they pass by or positions the stigma in such a way so pollinators will rub against it. The length of the style is different depending on the plant, helping the pistil weed out any incompatible pollen tubes when they fail to grow long enough to properly fertilize the ovules."
Source: homeguides.sfgate.com/part-flower-contains-stigma-style-6...
Stack of 211 images taken with the Nikon Plan Apo 4/0.2 160/-.
The extension had been increased to 185mm to result in 4,6x magnification.
Size of capsule before magnification approx. 1mm x 4mm. The capsules are the spore producing organs of the moss.
More Edinger-Westphal neurons in culture. Stained for Tubulin (green) and Synapsin (red). Of course, colocalization makes yellow. Imaged with a Zeiss Axioskop 2 FS Plus equipped with a Zeiss Axiocam HRm. 100x total magnification.
What you've got here is a blood clot. Red blood cells are trapped in the fibre bundles (fibrin, I believe) and held there to create a blockage. That weird cell towards the bottom left is committing suicide by apoptosis..
Here Ifound a spot were some scales were lost and one can see the base of some scales. they are were similarily build like birds feathers.
Stack of 291 images takes with the Nikon M Plan 100/0.75 SLWD 210/0.
This green algae Spirogyra has one of the most fascinating chloroplast shapes of all algae – a helical shape, or spiral. Spirogyras thrive in almost any freshwater environment and are a common species that is easy to find in shallow ponds, ditches and lakes.
For this photograph I stained some of the Spirogyra strands with a number of fluorescent dyes to highlight the spirally shaped chloroplasts. They were then mixed with natural strands and placed next to each other on a glass slide and photographed in fluorescent light.
Technical information:
Sony A9
Olympus UPlanSAPO 20x
ISO 250
0.3 sec
Accessories: Microscope
Post processing: Zerene Stacker, Lightroom and Photoshop. Cropped, haze removal, noise reduction, contrast enhancement.