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Another stack with the 200mm tube lens, so 25x magnification. This time I really tried to get the best out of the lens. The stack is composed of 909 images for a stacking depth of 105µm. All images were presharpened and denoised prior to stacking.
While the center resultion is really outstandingly high, the corner resultion is not satisfying.
Microscopy images taken during my fall quarter rotation in Stephen Smith's lab at Stanford. The technique is array tomography, which produces for these crisp, 3D, high resolution, large scale, many-channel fluorescence images.
smithlab.stanford.edu
Legend:
White: DAPI (cell nuclei)
Green: YFP (Subpopulation of layer V pyramidal neurons)
Purple: Tubulin
Blue/Red: Neurofilament
Orange: Myelin Basic Protein
Microscopic creatures that grow to about 0.5mm they are very hardy and can survive extreme temperatures, radiation, dehydration, starvation and even the vacuum of space. Taken through an apex practitioner microscope. Apex minigrab camera and 10x objective.
Candida albicans....the microbial agent of candidasis. Cell wall, nucleus and cytoplasmic organelles of viable yeasts, cultured for 24 hours on Sabouarud agar. (1000 X)
Triple phosphate crystals, in their characteristic "coffin lid" shape.
This is dog urine, but these crystals are found in human pee, too, and are not clinically significant, just cool to look at.
A sun animalcule spreads its deadly rays. Like tiny sticky conveyor belts, anything that comes in contacts with these death rays will be mercilessly drawn into the animalcule's cytoplasm and ingested. Rheinburg blue illumination, 160x optical enlargement.
The day I planned involved Roman roads and yomping up hills, but when I opened the curtains this morning it was so dreich ... that I've spent the day fiddling around with teeny tiny things under the microscope.
Isotomurus palustris
This specimen: www.flickr.com/photos/ajc1/24373876127/
100X, 14 images, Zerene PMax
Sony ILCE-6000
E PZ 16-50mm F3.5-5.6 OSS
f5.6 50mm 15s ISO 100
Electron Microscopy explores the microscopic world and introduces students to three types of microscopes used in identifying specimens too small to see with the naked eye. An overview and comparison of three different microscopes we have here at FWRI: the light microscope, scanning electron microscope, and transmission electron microscope.
Electron Microscopy explores the microscopic world and introduces students to three types of microscopes used in identifying specimens too small to see with the naked eye. An overview and comparison of three different microscopes we have here at FWRI: the light microscope, scanning electron microscope, and transmission electron microscope.
Microscopic creatures that grow to about 0.5mm they are very hardy and can survive extreme temperatures, radiation, dehydration, starvation and even the vacuum of space. Taken through an apex practitioner microscope. Apex minigrab camera and 10x objective.
Stack with Mitutoyo Plan Apo 10x/0.28 SL ∞/0 f=200
425 images.
I was lucky to have the opportunity to borrow the Mitutoyo from my employer in exchange for my Nikon CF Plan 10x/0,3 ∞/- for a while, because the Mitutoyo didn't work well with the Nikon 105mm Macro as tube lens. The CF plan works quite well with the 105mm as tub lens, although it is designed for 200mm tube lenses as well.
My tube lens is a resersed Elpro 1:1 (which has 200mm focal length). It seems to work very well with the Mitutoyo. Maybe I can convince my employer to officially exchange the lenses! ;-)
This butterfly has mostly transparent wings without any scales. Only at the base there are some areas with tiny scales, but even thses parts look qiute transparent to the naked eye.
Our 14 week old kitten was having a walk around the garden today when she chased something that was flying then squeaked and started licking her paw. It was a bee sting. It looked tiny but we got it out with tweezers and of course I had to put it under the microscope!
Taken with a Canon 1100D through a C Baker microscope with a DSLR adapater which slots into the eye piece holder. 6 images stitched together using Photoshop CS2 and tweaked using Fast Stone Image Viewer. Inset image shows the size of the sting in mm