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This photo and the following two amaryllis shots are my first experiments with "focus stacking"--taking multiple shots of the same subject with varying focal points from the closest point to the farthest point, then merging them into one image to maximize the depth of field.

Day 249 / 365 (#615)

105mm @ f/8.0, ISO100 1/100s

8 layer focus stack!!

Harmonia axyridis 4th instar larva

Materials from a school project in Evesham. Photos taken by students through the town and along the river were merged using the Focus Stacking tools in Photoshop. They were then printed out for use in a giant collage.

This represents 32 pictures covering a 1.8mm field of view of a crystal rock section of Spencer's.

 

I connected a stepper motor to the fine focusing knob on the Microscope with a bit of clear tubing - shaft to shaft - and then used a Gigapan control board to move the focusing up to capture 32 images.

 

I had to trigger the photos manually, and tell the Gigapan unit to advance to the next frame.

 

I now know how to remote trigger the Canon T2i camera, so getting focus stacking to be automatic, with automatic triggerig of the camera, is my next step.

After that I'll move on to xy stage automation

 

This is part of the nanogigapan project. See nanogigapan.blogspot.com

Canon EOS 70D

Canon MP-E 65mm f/2.8@5.6

Sigma EM-140 DG Macro Ring Flash,

33 shots stacked with Zerene Stacker

Discovered a collection of coins I had lost track of over the years. This is an 8 image stack.

Macro. 12 images stacked. The image is not compressed. Zoom in for details.

Mesosa nebulosa found active at night (2AM) on a dying Hazel tree branch (Corylus avelana)

Egg of the butterfly Pararge aegeria with the caterpillar visible by transparency. The caterpillar is completely compressed within its eggshell but it is nevertheless quite active making this set of pictures unsuitable for focus stacking... Each picture has been taken at 2-3 seconds intervals and on different focal plans.

Look at original size to see the animation :

www.flickr.com/photos/sanmartin/4914593548/sizes/o/in/pho...

 

You can see a more successful focus stack of this egg here :

www.flickr.com/photos/sanmartin/4914638332/

  

Materials from a school project in Evesham. Photos taken by students through the town and along the river were merged using the Focus Stacking tools in Photoshop. They were then printed out for use in a giant collage.

Male spider showing pedipalps. So hard not to blow out these white hairs. Also maybe less is more...too deep a stack makes it all a bit busy.

Fuchsia flower. Focus stacked from 3 shots

Rain drops on forsythia buds. Focus stacked from 2 pics

121 images taken with Canon 50D, Nikon CFI Plan 10x/0.25 objective, and 172mm achromat on Pentax Auto Bellows (10x). Taken at 10 micron steps with a Cognisys StackShot with two groups stacked in Zerene Stacker DMap + PMax. Lit with MT-24EX @ 1/16 with diffusion gels/dome.

First attempt at Macro focus stacking

Das Bild ist in zwei Teilen entstanden. Untere Hälfte 9 Bilder obere Hälfte 10 Bilder unterschiedlicher Fokus Ebene. Anschließend hab ich beide Bilder mit Autopano zusammen gerechnet. Objektiv M.Zuiko 300mm f4.0

Taken with Raynox 250; stack of 4 pictures.

I found a pair of butterfly wings lying on the ground about one or two feet away from each other. They belonged to an eastern tiger swallowtail.

 

A focus stack of 29 photos combined in Helicon

Materials from a school project in Evesham. Photos taken by students through the town and along the river were merged using the Focus Stacking tools in Photoshop. They were then printed out for use in a giant collage.

SONY SLT-A35 Sigma 70mm f:2.8 EX DG Macro

Focus Stacking

Nikon D200

AF Micro Nikkor 105mm f/2.8 D

CombineZP

Materials from a school project in Evesham. Photos taken by students through the town and along the river were merged using the Focus Stacking tools in Photoshop. They were then printed out for use in a giant collage.

Raindrops on bottlebrush flower. Focus stacked from 2 pics

from 6 images taken on the weekend during the storm in -6 degree weather.

Nikon D7100 + Sigma 105 mm macro

Day 348 / 365 (#714)

150mm @ f/8.0, ISO100 1/200s

Dead specimen found at the balcony. This one had its antennae broken and she was curled very tightly.

Focusstack,

108 Bilder, 108 Images Schrittweite/Step 0,05 mm,

L 6 - 8 mm

 

The Hornet. Very dangerous.

At home I have taken 31 shots to make focus stack of hornet's head.

 

Olympus digital camera, Focus stacking

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