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Just received it today. It was too expensive to ship the one made by Joel (Clockwork) from the US, so I found somebody who could CNC mill it for me. It also allowed me to customize it for my height gauge.
The design is very, very much inspired by this one ! :
www.flickr.com/photos/duncancycles/2578528943/in/set-7215...
"The Alignment Guy", the early years: Employee with a bad attitude by day, would be shop owner by night.
Sorry for the tiny photo. I can't find a larger copy, or the memory card the original is on.
Summary of cpcB.(A?B) cpcB mRNA levels during the life cycle of A. nidulans (A) and A. fumigatus (B). Conidia (asexual spores) are indicated as C. The numbers indicate the time (hours) after incubation in liquid MMG (Vegetative), and on solid MMG under conditions inducing conidiation (Asexual) or sexual development (Sexual). The relative band intensities (mean?SD) of Northern blot were determined by densitometric scanning of mRNA bands using ImageJ and each band was normalized by the amount of 18S rRNA (cpcB/18S rRNA) in the relevant lane. Ethidium bromide staining of ribosomal RNAs was used as a loading control. (C) Alignment of A. nidulans (Ani) CpcB, A. fumigatus (Afu) CpcB, A. flavus (Afl) CpcB with Gib2 of C. neoformans var. grubii (Cgr; accession: AY907679) and C. neoformans var. neoformans (Cne; accession: AY907680). ClustalW (align.genome.jp/) and BoxShade 3.21 (www.ch.embnet.org/software/BOX_form.html) were used for the alignment and presentation. (D) A phylogenetic tree of CpcB-like proteins identified in various fungal species including A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, A. terreus (A?=?Aspergillus), C. neoformans, Neurospora crassa, Penicillium chrysogenum, Penicillium marneffei, Schizophyllum commune, Schizosaccharomyces japonicus and Talaromyces stipitatus. The putative CpcB proteins were retrieved from NCBI BlastX (blast.ncbi.nlm.nih.gov/Blast.cgi) using A. nidulans CpcB. A phylogenetic tree of 14 putative CpcB homologues was generated by the TreeTop software (genebee.msu.su/services/phtree_reduced.html) using the alignment data from ClustalW. The phylogenetic tree is constructed based on the matrix of pairwise distances between sequences. Numbers indicate the computed distances given the residue substitution weights from the alignment data [59].
Noticed these misalignment issues when trying to assemble the line of pier and waterline (I thought it was me who was the problem!). These puzzles all have 3 vertical seams: between pieces 13/14, 25/26, and 37/38. Here you can see the middle and right-hand seam, and how the pieces are off alignment (but only in some areas, it looks like!). I have come to expect small alignment issues in puzzles, but it's pretty bad to see this in just a 2000 piece.
This hasn't completely ruined my puzzle experience, but I can already hear the complaints from fellow collectors. Making high-art puzzles is going to attract a picky audience. Probably a big reason why former art puzzle manufacturer Ricordi folded had to do with complaints from customers over quality issues. I remember reading many negative comments about Ricordi on Amazon, the German puzzle forum and from other sources, and when I finally got around to building some Ricordis last year I was surprised to find the quality decent if not top-notch. Grafika will need to do a better job than this to keep its customers - though on the other hand, art puzzle collectors are so desperate for new images that they may live with these issues. Wouldn't it be nice if we could have the best of both worlds: well-chosen art designs combined with exquisite manufacturing.
Megalithic tomb (Passage grave) Newgrange, Co Meath, Ireland - 2500BC. Dated to around 3200 BC, Newgrange is 1,000 older than Stonehenge and centuries older than the great pyramids of Egypt.
At the winter solstice, the shortest day of the year, the light of rising sun penetrates the tomb through the roofbox over the entrance, gradually illuminating the passage for a distance of 62feet to the three chambers at the end.
(I believe it didn't happen to day - too dark, damp and grey!). Photo taken several years ago.
Wheels: Anthracite SM-10
Front: 19x11" ET38
Rear: 19x12.5" ET55
Tires: Michelin Pilot Sport 4S
Front: 295/30-19
Rear: 325/30-19
Additional notes:
Stock Alignment
This lays at an angle so I clamped it with two forward baces so the joints are all unstressed when riveted
The new alignment at Heamies Farm, with Freightliner Class 66/5 No. 66506 'Crewe Regeneration' leading 4M88 0932 Felixstowe North - Crewe Basford Hall off the new alignment of the down slow from Searchlight Lane Junction on 17th August 2016. Previously a four-track formation continued around the curve to Norton Bridge, which now with closer inspection revealing that the now redundant fourth track (down slow-above red third container) has been lifted. Copyright Photograph John Whitehouse - all rights reserved
Sequence analysis of the PDIR non-catalytic domain.(A) Occurrence of the domain in protein disulfide isomerases and other proteins. Human ERp57 is shown for comparison. Catalytic motifs are shown in catalytically-active thioredoxin-like domains. (B) Rooted phylogenetic tree of proteins shown in panel (A). Sequences labeled WUBG_02370 and RNA methyltransferase are proteins from parasitic nematodes Wuchereria bancrofti (EJW86719) and Brugia malayi (XP_001896925); mosquito PDIR is from Aedes aegypti (XP_001659136). The N-terminal catalytic domain of ERp57 was used for the phylogenetic tree. The figure was generated with ClustalW [29] and TreeViewPPC [30]. (C) Sequence alignment of the non-catalytic domain from PDIR proteins from human (NP_006801), rabbit (XP_002716857), rattlesnake (AFJ50881), chicken (XP_422097), zebrafish (XP_001107048), frog (XP_001086600), fly (XP_609645), and sea urchin (XP_001200801) and the related sequence from Brugia malayi RNA methyltransferase (XP_001896925). The consensus sequence is shown below; the secondary structure elements are above the sequence.
© Copyright Paul Marsden. All rights reserved www.f29.co.uk
The Orion-theory that the alignment and placement of the pyramids of Giza is widely known, though much discredited.
Whilst reading up on the theory, i came across this, alternate theory to the alignment and layout of the pyramids of the Giza plateau.
Primarily they focused on something much closer than the Orion constellation, rather they set reflect the inner planets of our solar system: Mercury, Venus, Earth, and Mars.
This theory rests on several remarkable coincidences
1. the 3 misaligned pyramids have an angular separation equaling 191.6 degrees, or the identical angular distance Mars travels in one Earth year
2. the distances from the peaks of the pyramids are proportionally identical to the distance of the inner planets from one another
3. the three pyramid bases are directly proportional to the physical size for these four inner planets. The two largest pyramids have a size ratio very similar to Earth and Venus, while the third and smallest pyramid equals the "average" size ratio for the two smallest planets, Mars and Mercury
Thus some argue, their their main objective was to emphasize planet motion and the arrangement of the 4 inner planets.
This pyramid/planet comparison begs the question is it the result of a great engineering coincidence? Or were the pyramids constructed with the intent of reflecting the heavens -- and how did the Ancient Egyptians poccess this intimate knowledge of the Solar system, and how was it lost to mankind?
Read more: www.gizapyramid.com/CliveRoss.htm
The magnets do an amazing job keeping the TouchFire aligned.
This photo shows something else: silicone rubber grabs dust and hair. You can run it under water to clean that stuff off.
Read the full review here:
www.suntimes.com/technology/ihnatko/13715183-452/touchfir...
Sequence and expression analysis of GsWRKY20.(a) Amino acid sequence alignment of WRKY domains among GsWRKY20 and other type Ⅲ WRKY TFs, Sequences were aligned using ClustalW, and gaps were introduced to maximize alignment, filled triangle marks the cystine and histidine in the C2HC-type zinc finger domain.(b) The phylogenetic tree of the WRKY TFs. The phylogenetic tree was constructed using MEGA 4.1. Total 24 WRKY proteins from Oryza, Arabidopsis, Gossypium, Medicago, Triticum aestivum, Glycine max, Thlaspi caerulescens and Solanum were selected to construct the phylogenetic tree.(c) Tissue-specific expression analysis of GsWRKY20 by real-time quantitative PCR (qRT-PCR). Tissues included trifoliate leaf (TL), root (RO), stem tip (ST), flower bud (FB), and pod (PD). Expression of GAPDH was used as an internal control. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.(d) qRT-PCR analysis of GsWRKY20 diurnal expression under SD and LD. Trifoliate leaves were sampled every 4 h at 21 DAE. White and black bars at the top represent light and dark phases, respectively. Relative transcript levels were analyzed by qRT-PCR and normalized by GAPDH. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.
this is a panorama made from 8 pictures.
It was taken on May, the 1rst, at 3:00 AM GMT.
At this moment, Venus and Jupiter were in conjunction. The milky-way core was still visible.
Location : La Seyne sur Mer, near Toulon, France.
Setup : Nikon Z6 II + Nikkor 24/70 @ 24 mm -f/4 - ISO 1600 - 20 s
A Sydney Light Rail promotional team takes to George Street answering questions about the latest developments along the route. When I asked the obvious question, they reported that the crowds were "very supportive".
I am measuring any twist between the seattube and the headtube. .0035 inches is pretty much as good as it gets.
A view taken from Station Road, Mochdre in February 1984 where a brace of Metro-Cammell 2-car units was recorded working the BR Table 83 12:55 Llandudno Junction to Crewe. The train is running on the new track bed alignment installed to cater for the construction of the Landdulas to Glan Conwy [Colwyn By-Pass] section of the A55 North Wales Expressway.
All images on this site are exclusive property and may not be copied, downloaded, reproduced, transmitted, manipulated or used in any way without expressed written permission of the photographer. All rights reserved – Copyright Don Gatehouse
Somewhere in the Stockholm archipelago. Taken from the ferry between Nynäshamn and Nåttarö (also spelled Nåtarö, sometimes).
Ball technicians use specialized equipment to build the GPIM satellite so that the space vehicle instruments and thrusters align perfectly with the payload interface. Image credit: Ball Aerospace & Technologies Corp.
Sequence alignment and phylogenetic analysis of SKD1 proteins from Zea Mays and other species.A An alignment is shown for the deduced amino acid sequence of SKD1s from Zea Mays (Zm), Arabidopsis thaliana (At), Lycopersicon esculentum (Le), Oryza sativa (Os), Brachypodium distachyon (Bd), Hordeum vulgare (Hv) and Saccharomyces cerevisiae (Sc). The numbers on the left indicate the amino acid position. Identical residues in all these proteins are shown in a black background. Dashes indicated gaps introduced for optimal alignment. The putative MIT and AAA domains are underlined with a thick red line and a thick green line, respectively. B Phylogenetic tree based on SKD1 protein sequences from yeast, plants, and animals. The bootstrap values shown were calculated based on 500 replications. The tree was constructed using the neighbor-joining method. Z.mays, AY105155; A.thaliana, At2g27600; L.esculentum, AK324437; O.sativa, AF499028; B.distachyon, LOC100837561; H.vulgare, AK359160; D.melanogaster, NP_573258; G.gallus, AJ720732; H.sapiens, AF038960; M.musculus, NP_033216; S.cerevisiae, NP_015499.
Just joined Flickr Group Roulette (FGR) and today's theme... "In the Kitchen"... how perfect is that?!
A couple suitble
"Pre-Ses"
#1) My theme for the week is "Showing up and Staying the Course" and
#2) This writing was a result of journaling on the Question and Reflection from Gaia.com today... "When do you feel the most accomplished"
Cool things are happening today.
I just spent the last forty or so minutes in my kitchen, eating my breakfast (a bit late), scrubbing the sink, cleaning out the fridge – a task I started yesterday, taking photos, journaling and doing some sacred reading.
I don’t know when I felt so accomplished in so short of a time.
I don’t remember oatmeal with a smidge of brown sugar ever tasting quite as flavorful.
Right before my time in the kitchen I visited gaia.com where I oftentimes use the “Questions and Reflections” as a writing (and contemplative) prompt.
This is the question that came up:
“When do you feel the most accomplished?”
I wrote the question on the top page of my journal and began scrubbing. I knew when my initial scrub was over, I would go to my journal and write. I was having a splendid time, cleaning with the same passion I usually use for my art.
I sat down with my cup of coffee as my oatmeal cooked and wrote, “I feel most accomplished in this exact moment. I am on task witih my personal studies. I am working on my business, actively. My creativity and art is consistently flowing, my relationships are flourishing…
AND….
I had a huge a-ha yesterday (well, it was several yesterdays ago actually, on Friday, when I was seeing my therapist.) This “a-ha” springs from my home, my house, this place I have lived for nearly 18 years now.
I have come close to losing this house more times than I feel comfortable admitting. It is one loss God managed, somehow, to spare me from each and every time.
My house has sheltered me. My house has protected me. My house has been the place where I have grown as a human being in ways I never expected. My house has put up with my apathy towards it for wayyyy too long.
Now it is my turn – my long overdue turn – to fully love my house back. To show up and hug my house, cuddle my house.
It started several years ago, actually – on a New Years Eve when I painted my dining room a gorgeous shade of purple with a deeper purple ceiling and gold accents on the wood. I have continued in fits and starts, like in pulling up most of the carpeting last Summer revealing hardwood floors again. Now, I see the hugging, the cuddling, the caretaking of my house as a part of my complete and overall life alignment.
I see this alignment reappearing in so many ways.
Now, here it is, in my beloved home.
Today I scrubbed my kitchen sink with a toothbrush. This may seem ordinary for some of you. I have read about it on Flylady, but I have never actually done it myself. I knew of it, but I wasn’t intimate with it through experience, through touching, through the process. Today, I became intimate with the joy of scrubbing the sink with a toothbrush, working towards making it sparkle and shine. I think it even winked at me, although I am not completely sure.
Maybe that was Divinity winking at me.
My hand smells like cleaning solution.
Today that is what accomplishment smells like.
Dietrich Bonhoffer said, "Action springs not from thought, but from a readiness for responsibility."
I am ready for the responsibility of loving my home deeply and showing that love outwardly. It isn’t talking about “Oh, I can scrub my sink with a toothbrush” it is about showing up and doing it. And then repeating it. And being grateful for each part of the process.
Showing up in this way feels so good.
This seemed like a good idea at the time, but one of the screw heads for the machine heads sheared off. I was not happy.
Mating the microcontroller to the LED display, making sure where everything lines up.
Part of the high-tech holiday decorations project.