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Irish National Organisation of the Unemployed - Press Release:
Budget 2013 and unemployment
After much speculation Budget 2013 has finally been announced. The Budget does not do enough in two vital areas. It will make the path to work, education and training more difficult for unemployed people and will further undermine the social insurance model, a model that the Minister sees as integral to our social protection system.
As predicted the duration an unemployed person can stay on Jobseekers Benefit has been cut from 12 months to 9 if he or she has made over 260 PRSI contributions. Similarly, if the person has paid less than 260 contributions they will only now have an entitlement to a JB payment for 6 months. So, for example, a person who has worked for 20 years or more and whose partner is working may find themselves cut adrift from any supports from the State after nine months. Yet he or she will be unemployed, see him or herself as unemployed but they will receive no supports from the State to assist them to get back to work.
Yet the State will be taking more PRSI contributions off all workers as anyone earning over €352 per week will pay on everything after: this is a regressive step and will particularly hit low income workers.
Another striking feature of the budget is the cutting of supports for unemployed people undertaking further education and training. This will hit in particular young people for whom Jobseekers Allowance had been cut in the past to encourage them to take up education and training options. The rationale for offering participants a full rate was to support people to re-skill themselves, to improve their chances of securing employment. Yet the cut to the Cost of Education Allowance; the equalisation of a training allowance with a participant’s previous social welfare payment; and a maximum limit of €160 for young people will make it harder for people to go down this path as they will find it unaffordable.
Child Benefit has not only been cut by €10: it has also been cut from €148 for the third child to €130; and from €160 for 4th child+ to €140. The provision of additional childcare or early years supports may be of benefit to some families. However, for many families struggling on a social welfare payment or a low income job this cut in Child Benefit is further exacerbated by the cut in the Back to School Clothing and Footwear Allowance. This payment has been cut from €150 to €100 for children of primary age; and from €250 to €200 for children aged 12+. Yet the cost of education continues to rise. According to the last Consumer Price Index education inflation ran at 6.7% in comparison to a national average of 1.2%.
The INOU is acutely aware of the importance of local and community development in providing important supports and services to unemployed people including the provision of access to employment. At a time when increasing demand on the ground it is disappointing to see further expenditure cuts envisaged for these programmes.
On a positive note the maintenance of the basic social welfare rate, as sought by the INOU, is to be welcomed. As are the additional places in Community Employment, TUS, JobBridge: it is critical important that these supports are meaningful for participants and lead on to decent employment.
We hope you enjoy these images. Feel free to use them, all we ask is that you attribute them to "Kitmondo Vintage" with the link: www.kitmondo.com/equipment-knowledge-hub/the-vintage-mach...
We publish this material to provide historical insight on manufacturing and industry.
Prepping Nikon Picture Control types for use:
1. Using the Picture Control Utility in View NX2, for each Picture Control create a variant, with the following settings, (adjustments set to Manual in the Picture Control utility):-
Sharpening = auto
Contrast / Brightness = auto
Saturation = auto.
Note you first click the "Manual" button (to enable customisation) then proceed as follows
2. For each Picture Control type, name each (and save) as follows:-
Standard = as "Standard auto" variant
Portrait = "Portrait auto"
Etc. so that you have an auto variant for each Picture Control type, to get you started using them, in VNX2.
You can Export these pre-sets to your camera via SD card, refer to the help (ticked on the schematic.)
Once you have saved all the 'Auto' variants for each Picture Control type, then you can start to process your NEFs, using these saved custom settings. You can do this in the field when out shooting, or post the shoot, in VNX2 / CNX2.
Double click a NEF to bring it into the Edit window - then view the camera applied Picture Control type, then decide if you want to change / experiment.
You will or should notice a dramatic improvement in quality.
You will most often find very little extra sharpening is required.
N.B. Don't start messing around with curve customisation, until you know what you're doing and have built up experience with the customisation I've outlined at the top of this posting.
This billboard was advertising the new Mall Perks program at Century III Mall. I believe this was on Sawmill Run Boulevard.
Yvette Nicole Brown, Alison Brie and Ken Jeong speaking at the 2013 San Diego Comic Con International, for "Community", at the San Diego Convention Center in San Diego, California.
Please attribute to Gage Skidmore if used elsewhere.
Way expensive, no better than many cheap alternatives... and maker of the most annoying TV commercials.
Star Wars / Heft-Reihe
Flight into Fury!
cover: Carmine Infantino, Bob Wiacek
Marvel Comics Group (N.Y. / USA; 1979)
ex libris MTP
Ayer he Recibido el cuarto Pago de BeRuby México que había solicitado el mismo día, y que os recuerdo solicité a BeRuby México de 100 pesos mexicanos lo que... Puedes ver la entrada completa aquí: evitalacrisis.com/comprobantes-de-pagos/recibido-el-cuart...
Wheelman Free Download Wheelman in Direct Download Stuffs. Combining spectacular Hollywood-style stunts with a gripping storyline, Wheelman provides an adrenaline-fueled, cinematic thrill ride guaranteed to leave you breathless. Vin Diesel stars as an undercover agent and highly skilled driver who must infiltrate the Barcelona underworld to gather intelligence surrounding a covert heist. Posing as a ...
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The route travels through mostly unoccupied desert terrain, with much of its alignment paralleling the northern edges of the Nellis Air Force Range.
Snapped with a Pentax SP1000 from the city wall in Chester in monochrome (Ilford HP5), scanned from film negative.
I have always considered the ultimate in photography to be National Geographic Magazine. I was thrilled when I received a FlickrMail from them telling me that they wanted to use one of my images! The online edition, not the magazine. The German Online edition. This is a screen shot of the latest National Geographic Deutschland. I can read my name but that's about all. I don't read German. They told me it was it was for an article about the spread of Kudzu in the USA. It could be about really bad photography in the USA for all that I know.
But...either way....I'm in National Geographic! Cool!
www.nationalgeographic.de/wissenschaft/umwelt/umwelt-kamp...
This book concludes our tandem edition on Recombination and Meiosis. Subtitled Models, Means and Evolution, it follows its first-born twin with emphasis on Crossing-Over and Disjunction. In the commissioning of chapter topics we have tried to cover numerous aspects of the meiotic system from many different angles. Both these books are embedded as volumes 2 and 3 in a topical Series devoted to Genome Dynamics and Stability, where DNA transmission and maintenance functions are discussed from experimental and theoretical perspectives. The earlier vol. 1 dealt with Facets and Perspectives of Genome Integrity, focusing on DNA damage repair mechanisms, and an upcoming vol.4 is on transposable elements. These books on meiotic processes, together with other volumes in this Series on genome management in mitotic cells, provide a grass-roots level starting platform—initiating a prospective trajectory superimposable upon the exploding field of molecular cell physiology, or systems biology (see below). The preceding volume preferentially dealt with meiotic processes in multicellular organisms, such as plants and animals including man. Also, basic accomplishments from work on yeasts was presented in a comparative perspective—concerning the decisive roles of Spo11-induced breaks for crossing-over, of sister chromatid cohesion in chromosome disjunction, and cell cycle modulation in the global control of the meiotic program. The present book puts additional focus on yeasts as unicellular model organisms, where progress in revealing the mechanisms of meiotic recombination has taken place most rapidly and systematically. Also, a central aspect of genetic recombination in E. coli is included for its outstanding merits as a universal model. Furthermore, three facets of evolutionary relevance are also discussed. As for the models and means of meiotic recombination, two prominent and comprehensive chapters call for particular attention. Inasmuch as theoretical interpretations of empirical data about the exchange of genetical markers in successive generations has long preceded their biochemical elucidation,James E.Haber gives expert guidance on a veritable tour de force, presenting the Evolution of Recombination Models frompurely genetic crosses into the molecular era. He follows the historical record from simplistic breaking/joining schemes to break-induced replication, from suspected single-strand breaks to partner choice by single-strand annealing, and from the generation of double-strand breaks (DSBs) to their repair by the establishment and resolution of single or double Holliday junctions, and finally to DSB repair in the absence of crossing over accomplished through synthesis-dependent strand annealing that does not involve Holliday junctions. This scenic ride is aptly complemented from the enzymatic perspective, as displayed by Kirk T. Ehmsen and Wolf-Dietrich Heyer on the Biochemistry of Meiotic Recombination: Formation, Processing, and Resolution of Recombination Intermediates. These authors highlight the biochemistry of meiotic recombination, as more and more meiosis-specific enzymes have been added to the basic toolbox, which likewise is at work in mitotic cells (cf. GDS vol. 1, this Series). Overlapping with functions in replication and DSB repair these enzymes comprise topoisomerase, nuclease, recombinase, polymerase, and helicase activities, as well as single-strand stabilizing protein, a protective end-tethering complex and a range of modulating co-factors. The single most remarkable feature about the initiation of meiotic recombination is the deliberate and catalyzed introduction of numerous DSBs in the chromosomal DNA. Notably, the enzyme responsible for this pivotal and conserved activity is derived from a former topoisomerase (Spo11; Keeney, this SERIES), which as such had a cell-intrinsic function essential for the untangling of replication intermediates in every cell cycle. The total number of cuts is even larger than the number of effective crossovers later on2. The important question of how the sites to be cut are chosen in a given cell— among myriads of potentially equivalent sites that are ignored—is still one of the most vigorously pursued aspects of ongoing research. Foremost, the susceptible substrate for meiotic DSBs is not naked DNA, but DNA embedded in chromatin, as highlighted by Michael Lichten, in his chapter on Meiotic Chromatin—the Substrate for Recombination Initiation. The two yeasts compared for this traits how pronounced differences in the distribution of hotspot sites for DSB formation. In Saccharomyces cerevisiae, a fairly promiscuous DSB machinery can be assembled at about every stretch of accessible chromatin that has been opened up for other purposes, especially at activated promoter regions. Michael Lichten coins the term "opportunistic DSBs" for these phenomena, foremost in S. cerevisiae—differentiating meiotic DSBs from both lower
and higher degrees of sequence specificity: on one hand ionizing radiation induced DSBs,which occur with little sequence preference and without regard for chromatin structure, and on the other hand from the site-specific cuts of restriction-type endonucleases—or other nucleic acid transactions, such as transcription promotion, where both chromatinstructure and the recognition of DNA sequence elements contribute to specificity. Such opportunistic usage of promoter-modulated open chromatin can only in part explain the DSB pattern observed in the fission yeast Schizosaccharomyces pombe, where other determinants may play a significant, hotspot-specific role. Also to be determined by meiosis-specific chromatin organization, the assembly of and/or cleavage by the DSB machinery should not be all too promiscuous on a particular issue, in that at most one of two sister chromatids can become susceptible at any given site, whereas the other sister strand needs to be protected around the equivalent site. The molecular basis for this significant restriction still remains to be determined. After the meiosis-specific, Spo11-induced DSBs have been processed to protruding 3 ends, these single strands have to interact with the corresponding sequence on the homologous chromosome, in order to repair and seal the break by homologous recombination. In eukaryotes the crucial strand exchange reaction is catalyzed by RecA-like recombinases of the ubiquitous Rad51 family and/orthemeiosis-specificDmc1protein. As modeled by the most widely studied RecA recombinase of E.coli, Chantal Prévost, in herchapter on Searching for Homology by Filaments of RecA-Like Proteins, discerns their basic functions in the genome-wide search for complementary DNA strands so as to facilitate the initial strand exchange reaction in highly coordinated, helical DNA–protein filaments, which likewise are formed by the eukaryotic RecA homologs. Corresponding studies to the leading work on meiosis in S.cerevisiae have also been pursued in S.pombe,showing striking differences indetail at various levels. The most interesting aspects of this work are pointed out in two chapters specifically devoted to the fission yeast. For one thing, S. pombe belongs to the rather few organisms that have lost the ability to form synaptonemal complexes in meiotic prophase, which usually stands out as the most characteristic structural basis of bivalent synapsis. Instead, another conserved feature of canonical meiosis, the clustering of telomeres in the so-called bouquet arrangement, is vastly exaggerated in a series of nuclear movements, which in S. pombe facilitates a dynamical alignment
of homologous chromosomes from nuclear fusion throughout the entire prophase of meiosis (D.Q. Dingand Y. Hiraoka, this BOOK). Furthermore, the crossover mechanism itself is peculiar as well. Whilst many organisms including S. cerevisiae actually employ two partly overlapping crossover pathways, one of these pathways is entirely missing in S. pombe. Characteristically, the main recombinational intermediate in S.pombe consists of single Holliday junctions (G. Cromie and G.R.Smith, this BOOK), whilst earlier results on S. cerevisiae had suggested double Holliday junctions as the canonical model. The species-oriented chapter by Gareth Cromie and Gerald R. Smith, on Meiotic Recombination in S. pombe: A Paradigm for Genetic and Molecular Analysis,was published Online FirstinJune2007. At thatrelatively early date, most of their extensive data on DSB hotspot distribution in S. pombe were mentioned in brief as unpublished results. These significant data are now more fully discussed, as mentioned above, in Michael Lichten’s comparative chapter—with due reference to their recent publication in the mean time (Cromie et al. 2007). Unfortunate as such asynchrony appears to be, this is a price to pay for the advantages of Online First publication for the individual chapters as they are being completed—with a spread of Online First dates up to a year per book in such a series. Three evolutionary topics relating to meiosis have been selected to conclude this book: the putative origin of the meiotic system, the confinement of meiosis to the germline in animals, and the abandonment of meiosis in relatively few eukaryotic lineages, some of which are remarkably persistent on the evolutionary time scale—capable of lasting for millions of years. At the dawn of genetics, crossing-over and meiosis had been considered very much the same, but the early view of apparent congruence between the two phenomena has long since been abandoned. Instead, genetic recombination as such has proved to have much earlier and more fundamental roles than the complex and highly integrated pattern of mainstream meiosis, of which crossing-over has become the most characteristic ingredient. In short, homologous DNA recombination has directly co-evolved with faithful replication (see R. Egel and D.Penny, thisBOOK), clearing physical damageand/or broken replication forks as they arise (C. Rudolph, K.A. Schürer, and W. Kramer, GDS vol. 1, this Series)—potentially in each cell cycle of prokaryotes and eukaryotes alike. Of more sporadic occurrence, on the other hand, meiosis only happens once per generation,or life cycle—whatever meaning may be attached to these derived terms for unicellular organisms (see below). N.B., bacteria and archaea are proficient in recombinational repair of DSB damage to their DNA, but meiosis is missing altogether. In multicellular organisms, the meanings of generation and lifecycle are evident, and the complex inter-relationship of germline development and maintaining sexuality in animals and plants was already recognized by Charles Darwin and August Weissmann by the end of the 19th century. In his chapter on The Legacy of the Germ Line—Maintaining Sex and Life in Metazoans: Cognitive Roots of the Concept of Hierarchical Selection, Dirk-Henner Lankenau follows the germline concept to its historical roots, and he addresses the multiple levels of selective evolution related to this concept. Also, he fathoms Weismann’s prescient usage of germ plasm in its original meaning that nowadays has been replaced by genes and genomes—and he sketches a tie to modern frontiers, discussing the so-called nuage as a germline-specific germplasm organelle of multiple RNA processing, where a suspended term is thus revived in new guises. A hallmark of meiosis is the production of recombinant offspring, efficiently scrambling the parental genotypes. The overwhelming majority of taxonomic groups throughout eukaryotes show proficiency of meiosis, at least to begin with. Higher plants and animals would probably never have originated without the evolutionary thrust empowered by meiosis. Yet, sexual propagation including meiosis has been lost repeatedly in evolution, although major evolutionary innovations have never sprung from such secondarily asexual lineages. Hence, asexual lineages of relatively ancient origins can serve as virtual mirrors to reflect the evolutionary importance of meiosis in the remaining majority of animals and plants, as thoroughly discussed by Isa Schön, Dunja K.Lamatsch,
and Koen Martens in their chapter on Lessons to Learn from Ancient Asexuals. To single out a particular highlight, the purging of deleterious mutations by a meiotic recombination appears to be remarkably effective—readily compensating for the low mutation rates observed. As for the inferred origin of the meiotic system, this does not only far predate the emergence of multicellular animals, fungi and plants—it even dates back before the last common ancestor of all the eukaryotic phyla known today (LECA). As canonical meiosis, therefore, is a common heritage to all eukaryotes, there are no comparative cues among different lineages living today from which by parsimony to deduce a likely order of step-wise additions to the basic toolbox of meiotic mechanisms. On the other hand, the meiotic system is so complex in its widely conserved pattern, that its instantaneous invention from scratch appears unlikely. Against this rather uninformative backdrop, Richard Egel and David Penny, in their chapter On the Origin of Meiosis in Eukaryotic Evolution, propose a possible series of incremental steps towards meiosis, each of which could have added some selective advantage on its own. This series may well have started before the mitotic division system had been perfected to its present fidelity, e.g. when telomere-directed chromosome movements may have preceded the establishment of centromeres. Hence their hypothesis is subtitled Coevolution of Meiosis and Mitosis from Feeble Beginnings. A likely driving force to establish a proto-meiotic system—alternating with proto-mitotic nuclear division—is seen in maintaining a periodically needed dormancy program, so as to protect it against the accumulation of dormancy-deficient mutations at the higher error load presumed in early evolution. This is in line with the common correlation between meiosis and the formation of dormant spores or cysts in extant microbial eukaryotes. In a certain sense, therefore, a single generation in the life cycle of unicellular eukaryotes would last from one stage of encystment or sporulation to the next. With the commissioning and presentation of the various chapter topics on the genomic aspects of the meiotic system we hope to have served a salient need for integrating basic knowledge gained from studying diverse genetic model organisms. Research on meiotic exchange and segregation mechanisms may appear more esoteric than the vast resources spent on understanding metabolism and growth in mitotic cells. While emphasis on the latter area is motivated by the numerical predominance of mitotic divisions, as well as the direct connection of mitotic cell divisions to the immense problems of cancerous growth in human disease, meiosis in its paucity is more secluded and its medical aspects are limited to less pressing problems, such as impaired fertility or Down-like syndromes (H.Kokotas,M.Grigoriadou,andM.B.Petersen, this Series). Also, a certain twist of hierarchy is undeniable: whilst endless perpetuation of mitotic divisions can be viable as an evolutionarily stable strategy, a contiguous series of several meioses is certainly not. In this sense meiosis will always be the subordinate companion of mitosis. At the conceptual level, however, the complexity of molecular mechanisms applying to meiosis far exceeds that of its mitotic counterpart. And for the continuity of generations in most eukaryotic forms of life, both meiosis and mitosis are complementary features of general and essential interest. Traditionally, the largest share of meiotic research has been focused on DNA exchange and related features, whereas the immense field of protein–protein interactions in the rewiring of the meiotic cell out of and back into the mitotic cell cycle stood in second place. The concluding chapter of the preceding volume specifically deals with these meiotic aspects of molecular cell physiology (L. Pérez-Hidalgo, S. Moreno, and C. Martin-Castellanos, this Series). As pioneered with yeasts, genome-wide expression studies have started with identifying all the genes upregulated in meiotic cells and sorting them into functional categories. This is a long way off fromknowing all their particular functions. To illustrate the scope of the barely charted field: of 4,824 annotated genes in S. pombe, 955 proteins contain coiled-coil motifs4; of these, 180 are upregulated before, during or after meiosis—21 exclusively so, but not expressed during mitosis (Ohtaka et al. 2007). The interactive potential of so many proteins is enormous, and the systemsbiology of meiosis has merely just begun. To form a link between both books on Recombination and Meiosis, the list of chapter titles in the preceding volume is included after the Contents table of this book. In fact, as some of the individual chapters already had been published Online First, before the editorial decision to divide the printed edition into two books, the preliminary cross references had not yet accounted for the split. We apologize for any inconvenience this may cause, but the listing of all the chapter titles in both books should hopefully direct the reader to the proper destination. We would also like to point out that the missing chapter numbers are no neglect but reflect an obligatory compromise necessitated by publishing all manuscripts OnlineFirst immediately
after they have been peer-reviewed, revised, accepted and copy-edited (see, www.springerlink.com/content/119766/). We most cordially thank all the chapter authors for contributing to this topical edition of two accompanying books focusing on meiotic recombination. Without their expertise and dedicated work this comprehensive treatise would not have been possible. Receiving the incoming drafts as editors, we had the great privilege of being the first to read so many up-to-date reviews on the various aspects of meiotic recombination and model studies elucidating this ever-captivating field. Also, we greatly appreciate the productive input of numerous referees, who have assisted us in thriving for the highest level of expertship, comprehensiveness, and readability. We are again deeply indebted to the editorial staff at Springer. We would especially like to mention the editor Sabine Schwarz at Springer Life Sciences(Heidelberg), the deskeditor Ursula Gramm (Springer,Heidelberg),and the production editor Martin Weissgerber (le-tex publishing services oHG, Leipzig).
April 2008
Copenhagen, Richard Egel
Ladenburg, Dirk-Henner Lankenau
August 22, 1970. Ken Curtis, Milburn Stone, James Arness, and Amanda Blake of CBS's "Gunsmoke" (illus. by Jack Davis). Writing on cover by a previous owner of this issue.
Very very sad day today :(
A great man passed on :(
One of my all time favourite authors. A unique talent and perspective on the world :)
if Rangers are stripped of their EBT-funded trophies 12 different clubs will have won trophies in a decade
www.etsy.com/listing/85381310/black-swan-zine-book-print?...
I was watching an interview with the Rodarte Sister and the stylest for Black Swan. It was very interesting when they pointed out how the colors in Nina's (nathalie portman) clothing change through out the film. In the beginning she wears mostly pink with some blues and purple. Then in the middle she brings in brown into her wardrobe. And all the way to the end she wears black. I thought it would be cool to make a zine of Black Swan!
Info: print, 8"x5.5", 6 pieces of paper that are double sided, comes with my signature on the back.
Illustrations:
-there are ten illustrations
- A lot of cute and fun pages including: plain credits for the lead actors, Bio on Nina (Nathalie Portman's character), Nina's wardrobe, Quote from the movie(" I just want to be perfect"), illustration with subtitles and a few other pretty illustrations from photo's i found on tumblr.
If you have any comments or questions, please contact me :)
And if you have an request for a movie or a fashion icon zine, let me know!
you've got your ball, you've got your chain
tied to me tight, tie me up again
whose got their claws in you my friend?
into your heart I'll beat again
sweet like candy to my soul
sweet you rock and sweet you roll
lost for you, I'm so lost for you
oh and you come crash into me.
fun fact: this is my favorite song of all time.
Todos los derechos reservados, cualquier reproducción digital debe citar la fuente. Prohibida la reproducción total o parcial sin permiso.
Orchid is beautiful but "cold". This is just how I feel this flower :) though, according to the horoscope we should match. :)
Texture by darkwood67
Working on a project with the heading "Out of focus".
This is one of the ways I am attacking it....kinda reverse since the glasses make the text "in focus"...but still......most of it is out of focus.
I appreciate all comments!
BTW: If you recognize the book I will donate you 3 comments and 2 favorites ;-)
@designeour : 10 Stunning Green Walls That Make A Space Come Alive bit.ly/2ar3PvN bit.ly/2aex3ec (via Twitter twitter.com/designeour/status/758751126673948673)
what life is ?
• Life is whatever you make it, its a life of possibilities!
• Life is a miracle that was created by God.
• There is no real answer to what life is. There are many things that life is made up of and many characteristics of it too. Not even the best scientist can figure out exactly the answer to what life is
• Nothing, an...ywhere can answer this question because if you knew the answer to this question you would be either a fool or god sent
• Life is the prequel to the Apocalypse pronounced by the Lord of all Demons, Satan
• Life is really cool so just live it.
• Life is the presence of living organisms.
• No one really knows what the meaning of life is, so just live it to the fullest
• Life is wonderful. stop wondering what it is and live it
• Life is wonderful, yet is also conjures up amazing questions, as well as answers that will persevere throughout your life. So in other words, do continue to "live it", yet it's okay to have questions once in a while
• You always have a purpose to live. to use the life the good lord gave you!
• life is life nothing more, no god or religion can change that fact.
• the meaning of life is to live
• Life is a good thing
• Life is the song, love is the lyrics
• Life is natures experiments
• Life is a portal to death
• life is amazing spend the time you have wisely and be careful in dark places
A.Khorshidi