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Hi...I was playing this morning trying to use up some scraps and made this simple Valentine's day card.
thanks for Looking......
Simple pillowcase-style sundress. I shirred the top and used bias tape for the ties. I based the dress on this tutorial: fromanigloo.blogspot.com/2010/05/playful-days-double-laye..., except I did not make it double layered. (gets too hot for double layers in the summer)
blogged here: scatteredthoughtsofasahm.blogspot.com/2011/03/bias-tape-m...
SIMPLE MINDS
BIG MUSIC TOUR 2015
Official stage photographer
Le ZĂ©nith, Paris / FRANCE
20/11/2015
by Sandie Besso Photography
for any booking, professional & artistic shootings contact me : sandie.besso@gmail.com
Paris / France
Check out the rest of my shots of Simple Plan performing at the Bamboozle Roadshow in Cleveland, Ohio, right here!
Also take a look at my photos of Forever the Sickest Kids, Good Charlotte, All Time Low, Boys Like Girls, LMFAO, and Third Eye Blind!
SIMPLE MINDS
BIG MUSIC TOUR 2015
Official stage photographer
Le ZĂ©nith, Paris / FRANCE
20/11/2015
by Sandie Besso Photography
for any booking, professional & artistic shootings contact me : sandie.besso@gmail.com
Paris / France
Close up of the penholder. The best thing is that it is very easy to attach and remove so it doesnt interfere with my writing. It was a freebie so that makes it extra special.
K.I.S.S.
Keeping it simple. Tomatoes, grown in my garden... Simply.
Put it on a white background with sunlight through my window in the kitchen. The tomatoes are held together with a toothpick.
Bead standards # of molecules are:
beads0 0
beads1 3507
beads2 32348
beads3 196948
beads4 941222
Name: Liu, Jianping; Hioe, Catarina; Cammer, Michael
Date of imaging: 25 Jan 2012
Reference to date in paper notebook:
Goal: To measure number of molecules in cell membrane by comparing to fluorescent beads with known fluorophore concentration.
Microscope being used: Olympus IX70 with spinning disk confocal and Hamamatsu camera 4X4 binning
Objective magnification: 60X N.A. 1.42
Probes used and the names of the dyes: (example: rhodamine anti-beta tubulin from Sigma)
Channel 1: Alexa 488 "blue"
Channel 2: a few pics have Propidium iodide "green"
Cell type: "I have 4 samples, 293T cells are stained with
AF488 dye after having been transfected with SF162 env."
What is the substrate, for instance, if bilayers, what proteins are in the bilayers at what concentration for each condition?
Other treatments:
Explain the directory and file naming conventions:
beads4 are the most concentrated and beads1 are the least.
There were four dishes of cells, A through D. All the same.
I was playing with different backgrounds the other night and I liked how this one came out. this image has a natural reflection in. The background matte that I was using allowed the water to pool and thats how I was able to get the reflection.
Go Ahead....View On Black