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Four shots like this one, always the opaque part of the microscope slide barely broke.
Interesting, the rough surface let the glass more resistant?
Usually I would think the opposite.
A mm ruler-each line is 1 mm from the next.
Canon T2i, medium resolution still photo
3456x2304 pixels.
444 pixels/mm
40x 0.65NA objective. HD video focus stacked.
Tried an oblique light from the top, which really doesn't do the subject any favors
Panasonic LX3 coupled to microscope to image a diamond ring. Nine images stacked and combined for increased DOF using Deepskystacker. LX3 set to RAW, f/4, ISO 200, 1/25 sec.
40x objective, NA 0.65 plus 2.5x relay lens to Canon T2i
captured as HD Video and then focus stacked.
Scale ~8.7 pixels per micron.
Theoretical resolution should be ~0.5 microns
AB Test of Focus Stacking
One stacked from 120 images in about 3 1/2 minutes.
The other stacked from 12 images in 18 seconds.
Can you tell a difference?