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I used my sons microscope and attached my canon 750d to get thiS 5 second macro shot of a spine at the base of a courgette from my garden.
Another view of a vintage Spencer Buffalo Stereo microscope, circa 1932. I think this may be a model 55. Spencer is believed to be the earliest successful American microscope manufacturer.
After 25 years I have finally replaced my old Stereo and Compound Microscopes. My old microscopes were of good quality and they had served me well over the years, but I have long missed the research grade microscopes that I had used while working for the Federal Agencies. It was time for an upgrade.
The one of the right is a 3.5x-180x Simul-Focal Trinocular Stereo Microscope and the one of the left is a 40x-2000x Quintuple Brightfield Phase Contrast Plan Infinity Compound Microscope.
Both have advanced LED Fiber Optic lighting systems. I also upgraded to a 18MP Microscope Digital Camera that can be used on both scopes (it is on the Stereo Microscope in the Photo).
I have been checking them out in detail over the past week. So far they have preformed as I had hoped. I am more than pleased with my new toys.
I love to try some different budget microscope lenses. Some of them are great performers like Lomo (3.5X, 3.7X, 4.7X) and Nikon 10X 0.25
Top row from left to right
-Lomo 8X 0.20
-Lomo 10X 0.30
-Lomo 20X 0.40
-Lomo 40X 0.65
-Nikon 10X 0.25
-Noname Plan 20X 0.40
-Noname 40X 0.65
Second row from left to right
-Lomo 3.5X 0.10
-Lomo 4.7X 0.11
-Lomo 3.7X 0.11
-Lomo 3.7X 0.11 again
-Nikon 4X 0.10
-Noname 4X 0.10
-Noname 10X 0.25
I took the shot with the help of a cheap M42 extension tube set fitted with a Lomo 3.7X microscope objective lens,
Used two diffused led desk lamps for illumination.
I don't like messing with flies you don't know what they have been doing.
I wouldn't hang it on the wall.😊
A microscope (from the Ancient Greek: μικρός, mikrós, "small" and σκοπεῖν, skopeîn, "to look" or "see") is an instrument used to see objects that are too small for the naked eye. The science of investigating small objects using such an instrument is called microscopy. Microscopic means invisible to the eye unless aided by a microscope.
There are many types of microscopes, the most common and first to be invented is the optical microscope which uses light to image the sample. Other major types of microscopes are the electron microscope (both the transmission electron microscope and the scanning electron microscope) and the various types of scanning probe microscope.
The first microscope to be developed was the optical microscope, although the original inventor is not easy to identify. An early microscope was made in 1590 in Middelburg, Netherlands.Two eyeglass makers are variously given credit: Hans Lippershey (who developed an early telescope) and Zacharias Janssen. Giovanni Faber coined the name microscope for Galileo Galilei's compound microscope in 1625 (Galileo had called it the "occhiolino" or "little eye").
Image of objective lenes and covers on blank spaces in nosepiece turret on a Nikon inverted microscope. Black and white image
Inspecting a record with a microscope
1970
fortepan.hu/hu/photos/?id=57570
Fortepan / Fortepan
Leírás
Magyarország
Magyar Hanglemezgyártó Vállalat, minőségellenőrzés.
Év
1970
Képszám
57570
Fotó adományozó
Fortepan
Kulcsszavak
karóra, hanglemez, mikroszkóp
Kulcsszó hozzáadása
This is a micrometeorite. Measuring between a quarter and a third of a millimeter in diameter, it is one of the smallest gems I have had the privilege of photographing. At such small scales, very specialized equipment needs to be used to get photographic results like this – but wait – did you say micrometeorite?!
This porphyritic micrometeorite is on loan to me from Scott Peterson, who has a passion for hunting these down; often found on the roofs of shopping malls with techniques and extra information available on his website ( micro-meteorites.com/ ), Scott and I have been discussing these gems for some time. As Scott puts it, “Porphyritic micrometeorites were exposed to lower temperatures on the scale. They were heated to around 1600 degrees C. Because of this “low” temperature it allows for this specific arrangement of crystals to form, internally and externally”. I might add, I see lots of tiny bubbles too!
Much to my surprise, these microscopic gems actually originate as tiny (albeit larger than their final form) meteors in space, not broken-off pieces of larger rocks that burn up in the atmosphere. Scott does a great job at describing this also: “The size is based on what Mother Nature will allow. If too small then they will burn up, if too “large” they will burn up. So the average size is .2-.4mm. Although there are smaller and larger ones”. This particular specimen has an uncommon make-up that allows it to be translucent, and at least somewhat hollow. Because of the mostly non-metallic ingredients at play, it became very alluring for me to photography… but where do you start?
This was shot with a Mitutoyo Plan APO 50x microscope objective to gather has much possible detail from the subject as possible. Microscope objectives have a number of key features to consider including their numerical aperture and working distance, and this was the best bet to use a combination of reflected and transmitted light to image. This particular objective has a numerical aperture of 0.55 and a working distance of 1.3cm, giving just enough room for light to be shaped in front of the subject. I could have gone with a slightly wider aperture for higher resolution, but the working distance would fall to an unusable 5.2mm (for this kind of work, anyhow). With a maximum resolving power down to 0.5 microns, I set to work.
It’s important to note that I also have a 20x objective with a resolving power of 0.7 microns. More than doubling the magnification to 50x only increases the true resolution by maybe 30% at best. Higher-megapixel cameras are useless in this scenario, because the resolution bottleneck occurs far away from the sensor!
It’s trivially easy to shoot with a microscope objective – especially at lower magnifications. These objectives are infinity corrected with a label of “f=200” on the side. Effectively, if you attach this to a 200mm camera lens with a step-down filter, set the camera lens to focus at infinity, boom. You’re now shooting with the microscope objective. I used an ancient Canon FF 200mm F/4 lens as the camera lens, which was really the cheapest thing I could find. The Canon lens as well as the Mitutoyo objectives are all eBay purchases – lots of great second hand material for these experiments, with 2x objectives being by far the least expensive.
The setup: three flashes off camera, lying flat on the table to shoot horizontally into the subject. I cut a pie-shaped wedge out of a translucent water bottle cap as a light diffuser, placed over top of the micrometeorite where the open “slice” was to shoot the subject clearly. Modelling the light and finding the best subject angle was, in a word, nightmarish. No substitute for trial & error experimentation here. And then comes the focus stacking.
This would be impossible without a good focusing rail. I used a Cognisys Stackshot which is automated, it took 450 shots of the subject of which I used 428 (throwing away a few out of focus shots on either side). Processing that many shots for these subjects, my preferred software is either Zerene Stacker or Helicon Focus; I always try them both to see what gives the best results, and Zerene Stacker (zerenesystems.com/cms/stacker ) tends to handle micrometeorites the best. It offers a nice high dynamic range output which ensures that highlights are not over exposed or shadows too crunched, like Log video footage that needs to be graded as a secondary step. After a few hours of massaging pixels in post-processing, this is what you get.
Oh, this will be in my upcoming book too. :) If you haven’t check that out yet, you’ll love it if you’ve read this far: skycrystals.ca/product/pre-order-macro-photography-the-un...
Hi Greyframe!
As you asked - that’s my microscopic equipment. I use an vintage (from the 70ies) Zeiss (West) Standard RA which is equipped for DIC (differential interference contrast), phase contrast, polarization and for good, old, bright field. For DIC I use planachromatic objectives and for bright field I use planapochromatic ones, if possible.
It’s old stuff but these gadgets are very robust and they are still working perfectly well. Moreover, their level of optical performance is not so far away from contemporary benchmarks. Many of these old microscopes are still in use in research facilities all over the world.
My camera is a Canon EOS 70D and I combine it with an old Leitz Elmarit-R 35mm. Microphotography easily pushs optics to it's limits and an Elmarit simply yields a slightly better resolution than Canon lenses. The difference is not big, but you can spot it nevertheless. For microscopic sessions I use live-remote-control-mode, that’s much more comfortable than anything else.
Zeiss ceased production of these microscopes at the end of the 80ies or so, so their original camera-adapters don’t fit to digital DSLRs. Fortunately I found someone who was able to build me a fitting adapter. Quite the same is true for the lamp - I use a LED instead of the original light bulb and this contraption was built and adapted by another microscopy buff.
Ah, and I use a software called Zerene for stacking. It’s output still needs some adjustment of contrast and brightness and some cleaning up, too. Nevertheless, if you don’t have microscopic pictures of high quality and high resolution as an input you cannot end up with a good picture in the end. Photoshop cannot compensate for bad resolution and so on.
OK, I don’t know whether all your questions are answered by now - if not, just ask. Maybe, my answers will come with some delay but there will be answers :-)
Micro-photography set up.
Compound microscope with built in digital camera and DSLR attachment. LED lighting for photographing non opaque objects under high magnification.
Stereo microscope with similar features. both microscopes linked to laptop.
Here’s the microscope with the forgiving end of a Ticonderoga #2 for reference.
- - - - -
Created for the Looking Close… on Friday theme, MINIATURES.
This creation uses 4 LEGO magnifying glasses to create a fully functional compound microscope. Other features include separate coarse and fine adjustment knobs, power functions LEDs and a rotating stage.
Now on Cuusoo:
lego.cuusoo.com/ideas/view/44370
Custom logo print by Dan Kees:
Inktober, Day 6: Wings and scales of a tiny moth at 40x and 400x
Tombow brush pens (black and brown)
Spectra 60 percent warm gray marker
Strathmore Drawing smooth surface
35.6 x 27.9 cm (14 x 11 inches)
This is a partially disassembled microscope made by E.F. & F.H. Tighe, Detroit, Michigan, possibly their No. 5 version, ca 1889.
It had been dropped while in its box from a height of about three feet thoroughly jamming the focusing mechanism. A friend wanted to know if it could be repaired since it has great sentimental value. The focusing mechanism was jammed to the point where it was locked, producing no tube movement, but a definite "skip" as the pinion gear engaged the rack. Fortunately, the rack is actually made from a slightly bowed thin piece of brass... not a thick solid piece. Anchored with screws at either end, the rack has a bit of springy flex to it, preventing gear or rack damage when forced. Getting the tube off the focusing assembly without damaging either was accomplished by directing a thin stream of hot air onto the focusing block, then precisely applying WD-40 with a blunted toothpick. Once the tube was removed, small bits of brass were cleared away and the slides cleaned and polished. De-gunking the surfaces of the slides was difficult because they had a thin coating of hardened black grease that would not move using any kind of cleaner. Cotton swabs dipped in boiling water finally softened the stuff to the point where "Nevr-Dull" brass cleaning wool was able to do the job. Reassembled, the scope is now back in its original configuration and looks great. Some folks have been known to clean off any protective lacquer and polish the brass to a mirror finish. That would be OK if this was still in the maker's display case waiting to be sold, but would totally destroy the nice patina that 130 years of aging has produced.
DSC-5435
Microscope lens setup. Effectively extension tubes and a extension tube to RMS cone adapter giving approx 160mm from focal plane to objective. A USB mini LED lamp fed from a rechargeable battery block (that goes in my pocket). The disc near then of the cone is actually a twin flash mount.
Parts. Sony A6000, Sony to EOS lens adapter, EOS to M42 adapter, M42 extension tubes, M42 to RMS cone, 4X 160mm microscope lens.
I already had these but obviously the adapters depend on the camera body in use but you need to get to M42 for the RMS adapter. The most common RMS adapter is a flat disc, if you use this you will need to use more M42 extension tubes.
Euplectus brunneus (Coleoptera, Staphylinidae).
Studio work about the same beetle from the previous post. 150 shots takenwith a Nikon CF Plan 50x microscope objective at around 50x magnification. Stacked in Zerene Stacker.
Description: Sophie Lutterlough, hired in 1943 as the Smithsonian's first female elevator operator, sits in front of a microscope in the Department of Entomology. After 40 years of service to the National Museum of Natural History, Lutterlough retired in 1983. For 14 years Lutterlough ran the elevators, but moved on to a second career after asking Dr. J. F. Gates Clarke if there were any openings in his department. Clarke hired Lutterlough as an inspect preparator. During the next 26 years in the department, she took college level courses to increase her knowledge of entomology and learned German to help with her work. Featured in Smithsonian newsletter, TORCH.
Photographer: Harold E. Dougherty
Date: July 1983
Image ID: SIA2009-3239
Collection: Historic Images of the Smithsonian
Link to Original: siarchives.si.edu/collections/siris_sic_12130
Repository: Smithsonian Institution Archives
"R. & J. Beck, opticians, No.1016 Chestnut Street, Philadelphia, Pa. W. H. Walmsley, manager. Spectacles, eye glasses, opera glasses, microscopes, thermometers, telescopes."
For another trade card from this company, see R. & J. Beck, Opticians, Philadelphia, Pa. (thumbnail image below).
Originally posted on Ipernity: W. H. Walmsley, Manager, R. & J. Beck, Opticians, Philadelphia, Pa.
Hi Greyframe!
As you asked - that’s my microscopic equipment. I use an vintage (from the 70ies) Zeiss (West) Standard RA which is equipped for DIC (differential interference contrast), phase contrast, polarization and for good, old, bright field. For DIC I use planachromatic objectives and for bright field I use planapochromatic ones, if possible.
It’s old stuff but these gadgets are very robust and they are still working perfectly well. Moreover, their level of optical performance is not so far away from contemporary benchmarks. Many of these old microscopes are still in use in research facilities all over the world.
My camera is a Canon EOS 70D and I combine it with an old Leitz Elmarit-R 35mm. Microphotography easily pushs optics to it's limits and an Elmarit simply yields a slightly better resolution than Canon lenses. The difference is not big, but you can spot it nevertheless. For microscopic sessions I use live-remote-control-mode, that’s much more comfortable than anything else.
Zeiss ceased production of these microscopes at the end of the 80ies or so, so their original camera-adapters don’t fit to digital DSLRs. Fortunately I found someone who was able to build me a fitting adapter. Quite the same is true for the lamp - I use a LED instead of the original light bulb and this contraption was built and adapted by another microscopy buff.
Ah, and I use a software called Zerene for stacking. It’s output still needs some adjustment of contrast and brightness and some cleaning up, too. Nevertheless, if you don’t have microscopic pictures of high quality and high resolution as an input you cannot end up with a good picture in the end. Photoshop cannot compensate for bad resolution and so on.
OK, I don’t know whether all your questions are answered now - if not, just ask. Maybe, my answers will come with some delay but there will be answers :-)
A microscope (from the Ancient Greek: μικρός, mikrós, "small" and σκοπεῖν, skopeîn, "to look" or "see") is an instrument used to see objects that are too small for the naked eye. The science of investigating small objects using such an instrument is called microscopy. Microscopic means invisible to the eye unless aided by a microscope.
There are many types of microscopes, the most common and first to be invented is the optical microscope which uses light to image the sample. Other major types of microscopes are the electron microscope (both the transmission electron microscope and the scanning electron microscope) and the various types of scanning probe microscope.
The first microscope to be developed was the optical microscope, although the original inventor is not easy to identify. An early microscope was made in 1590 in Middelburg, Netherlands.Two eyeglass makers are variously given credit: Hans Lippershey (who developed an early telescope) and Zacharias Janssen. Giovanni Faber coined the name microscope for Galileo Galilei's compound microscope in 1625 (Galileo had called it the "occhiolino" or "little eye").
Tiny shell photographed with a microscope using a 2,5x objective. Apprx 6 panels stitched in microsoft ICE. Had to drastically reduce the image size for uploading.
A present from my cat, who brought the shell inside stuck to her fur, so i realised i had a new subject to shoot. :)
Leitz SM Compound Microscope.
Camera: Rolleicord Va Type 2
Lens: Schneider-Kreuznach-Xenar 75mm f3.5 with Rondo Close-up attachment II.
Film: Kodak T-Max 100
Developer: Beerenol (Rainier Beer)
Botryoidal specimen of cryptomelane on goethite from Ironwood, Gogebic County, Michigan, USA, on display at the AE Seaman Mineral Museum, Michigan Technological University, Houghton.
Cryptomelane is a near-surface manganese mineral formed during the weathering of manganese-bearing iron oxide-rich rocks. This grey beauty is actually two minerals. The bubbly grey stuff is cryptomelane which is composed of 1 potassium + 8 manganese + 16 oxygen molecules. Goethite is the rusty brown underlying plate of iron oxide.
"Supergene" or "alteration" or "secondary" minerals are the fun stuff, because they form unusual or colorful or beautiful specimens. The word "supergene" is used to describe conditions of low-pressure and low-temperature during "weathering". The Low-P (pressure) and low-T (temperature) supergene minerals form in near-surface conditions versus High-P and High-T minerals formed deeper in the Earth's crust. Alteration by element-rich near-surface water will form new hydrated (supergene) minerals. Such mineral-forming alteration is time-, place- and element-specific. And instead of being eroded, carried away and recycled, some altered rock formations may be subsequently buried and preserved if in a sedimentary environment.
= Primary Rock-Forming Minerals versus Secondary Minerals =
The rocks in the Earth's crust consist of 12+ primary minerals.
The remainder of ongoing identification of 6000+ minerals (MINDAT website) are all of the other elements in the periodic table kept busy throughout time combining and recombining with primary rock-forming minerals.
The 12+ primary rock-forming minerals form the floating granite-dominated continents comprising 30% of Earth's surface, whereas the basalt-dominated ocean bottom crust is the hidden 70%. Perhaps 500+ minerals have some abundance on Earth out of 6000+ known minerals. Which means most minerals are extremely rare, usually tiny in size and mostly seen by microscope or identified/characterized by scanning electron microscope (SEM).
So a hand-sized supergene mineral specimen is very cool !!
dug out a box of old microscope bits and stuck one onto the front of the camera - not the best but it still surprised me as I didn't really expect anything very much...
There's a few ways of doing this (it's not my design by the way) and I'm sure you'll find a few on flickr. As long as you've got the curved brick and the binoculars it'll look good.
When i study in medical college, I have a white ZEISS microscope. Althogth my pathalogy was down by my professonal. I stll like my microscope. But i am graduated for 9 year and be a father. So i must sell it to buy more LEGO brick for my child, in fact, these brick are for me. :)
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