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Authors: DiÄdem Aktopraklıgil Aksu, Abdulrezzak Memon
Abstract: Wheat is one of the most important cereal crops in the world. Many parts of the world depend on wheat as a source of food and animal feed. Drought stress negatively affects its development and greatly reduces its production. Drought response is a complex genetic mechanism involving multiple genes, transcription factors, miRNAs, hormones, proteins, co-factors, ions, and metabolites. Understanding the genetic basis of the drought tolerance mechanisms is very important for genetic improvement of this trait in wheat. Wheat is also an important cereal crop in Turkey. In this study, it is aimed to identify drought stress-regulated genes in bread wheat (Triticum aestivum cv. Gerek 79) and gene expression profiling using mRNA differential display (mRNA DD) was performed for seedling leaves of control and drought-stressed plants (62.4% of RWC). The comparative profiling study showed a total of 20 differentially- expressed cDNA bands and 10 of them were cloned and sequenced. The inserts with poor quality reads were eliminated. The nucleotide sequences of the remaining two cDNAs named G1 and G2 were subjected to similarity analysis. G1 and G2 showed a high degree of homology the mRNA of purple acid phosphatase and glycosyltransferase family 92 protein-like sequences of Triticum aestivum and some other plants, respectively. Purple acid phosphatases have been shown to be involved in plant responses to abiotic and biotic stresses. Similarly, the role of glycosyltransferases in thermotolerance has been reported in rice besides their functions in cellular homeostasis and detoxification pathways in plants. These reports and our findings have laid a foundation for further investigation of G1 and G2 cDNA clones. The investigation of differential expression of these gene fragments corresponding to purple acid phosphatase and glycosyltransferase family 92 protein-like sequences under drought conditions at the RNA level is ongoing. Further characterization of these genes could be important in understanding the functions of these gene/s in drought response.
Keywords: Wheat, drought, mRNA differential display, gene expression
Authors: HörĂŒnaz ErdoÄan, Ä°brahim Aydın, Fatih Alay, Ferat Uzun, Ămer Faruk Uzun
Abstract: In the study, the equal to a law of rangeland parcels numbered 467 and 567 in Atakum, SarıtaĆ neighbourhood, which was declared as "Urban transformation and development project area" by Samsun Metropolitan Municipality, for the change of allocation purpose was investigated. The study, in which the rangeland condition class was determined by examining the vegetation of the rangeland parcels by visual estimation method, was carried out in the second half of May 2022. According to the vegetation survey data, the conditions of the rangeland parcels numbered 467 and 567 were in the "Good" and "Medium" classes with 59.14% and 42.48%, respectively. According to the 14th article of the rangeland law, it is not possible to change the purpose of allocation in the parcel numbered 467, but it is understood that a change in the purpose of allocation can be made in the parcel numbered 567. In terms of topography, it was seen that parcel no. 467 had a rough surface with slopes varying between 3% and 15%, parcel no. 567 between 5% and 35%. Although the slopes of both plots were sufficient for small cattle to graze easily, it was determined that plot number 467 was suitable for cattle, but plot number 567 was restrictive. Lotus corniculatus, Trifolium fragiferum, Trifolium repens, Trifolium hybridum, Medicago sativa, Poa pratensis, Lolium perenne, Dactylis glomerata and Sanguisorba minor, which are high quality forage plant species identified in rangeland plots, can be recommended as primarily preferred species in pasture plant mixtures for livestock enterprises in the neighbourhood.
Keywords: Plant composition, urban planning, urban development, rangeland condition, rangeland law, real estate law
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Author: BarÄ±Ć Kurt
Abstract: The main protease (Mpro or 3CLpro) plays important roles in viral replication and is one of attractive targets for drug development for SARS-CoV-2. In this study, we investigated the potential inhibitory effect of lycorine molecule as a ligand on SARS-CoV-2 using computational approaches. For this purpose, we conducted molecular docking and molecular dynamics simulations MM-PB(GB)SA analyses. The findings showed that the lycorine ligand was successfully docked with catalytic dyad (Cys145 and His41) of SARS-CoV-2 Mpro with binding affinity changing between -6.71 and -7.03 kcal mol-1. MMPB(GB)SA calculations resulted according to GB (Generalized Born) approach in a Gibbs free energy changing between -24.925-+01152 kcal/mol between lycorine and SARS-CoV-2 which is promising. PB (Poisson Boltzmann) approach gave less favorable energy (-2.610±0.2611 kcal mol-1). Thus, Entropy calculations from the normal mode analysis (ÎS) were performed and it supported GB approach and conducted - 23.100±6.4635 kcal mol-1. These results showed lycorine has a druggable potential but the drug effect of lycorine on COVID-19 is limited and experimental studies should be done with pharmacokinetic modifications that increase the drug effect of lycorine.
Keywords: Molecular Dynamics Simulation, MMPBSA, Gibbs Energy, SARS-CoV-2
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Authors: Nurmuhammet TaĆ, Muhammet Emin Naldan, Fatih Ăner, Yener Aydın, HĂŒlya Naldan
Abstract: This study evaluated cases of spontaneous pneumothorax developing secondary to SARS-CoV-2 pneumonia. Sixteen cases presenting to our hospital due to spontaneous pneumothorax developing secondary to SARS-CoV-2 pneumonia between March 2020 and February 2020 were evaluated retrospectively. Ten patients (62.5%) were men, and six (37.5%) were women, with a mean age of 68 ±20.3 years (range 18 - 90 years). Pneumothorax was in the right hemithorax in 11 cases (68.75%), in the left hemithorax in two (12.5%), and bilateral in three (17.75%). Pneumothorax developed during active SARS-CoV-2 pneumonia in all 16 cases (100%). No pneumothorax was detected following the healing of SARS-CoV-2 infection. Pneumothorax was observed while patients were not intubated in 15 cases (93.75%), but pneumothorax developed during mechanical ventilation in one case (6.25%). Tube thoracostomy was performed on all patients in treatment. Air leakage from the tube was observed in 14 cases (87.5%). The mean duration of tube thoracostomy was 18.3 ±20.1 days (range 1 - 81 days). Pneumothorax resolved after treatment in seven cases (43.75%), while mortality occurred in nine (56.25%). Pneumothorax recurred after treatment in one case (6.25%). Pneumothorax is widely seen in the active period or after healing in cases infected with COVID-19. Aggressive treatment is generally required for this clinical manifestation with high mortality.
Keywords: SARS-CoV-2 pneumonia, pneumothorax, management
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Authors: Burhan Budak, Erhan Dinckaya
Abstract: In this study, in respect to provide a novel and inventive viewpoint, a new assay for analysis of L-ascorbic acid enhanced using PGE. Ascorbat oxidase was crosswise bounded using gluteraldehyde and gelatine, stable to the surface of pencil graphite electrode and devised biosensor used for the detection of ascorbic acid. L-ascorbic acid measuring done amperometrically at -0.7 V using the improved biosensor. For the PGE/gelatine- glutaraldehyde /ascorbate oxidase biosensor ascorbate oxidase concentration, holding time in glutaradehyde, gelatine amount and glutaraldehyde stratifying repat cycle determined to be 1.5 U/mL, 3minutes, 20 mg and 3 times stratification in turn after optimization works. Potassium phosphate buffer (pH:7, 50 mM) and 30ÂșC warmth determined to procure optimum labouring conditions. After the characterization labours, 25 ”M - 500 ”M detection space provided to be linear for PGE/gelatine- glutaraldehyde/ascorbate oxidase biosensor. Regarding the results, the % coefficient of variation (V.K) = 0.44 and the standard deviation (S.S) = ±1.46 ”M. As a result of the experiments on storage stability, it determined that 75% activity was preserved at the end of the 4-week period.
Keywords: Pencil graphite electrode, ascorbate oxidase, amperometric, biosensör, ascorbic acid
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