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Definitely amongst one of the most difficult and time-consuming jobs in a college is preparing a research papers. But do you know what needs to be done before you even think of starting off with your favorite topic for research? You need to submit a research proposal.
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Authors: Alpgiray TURGUT, Ayşenur YAZICI, Mesut TAŞKIN, Serkan ÖRTÜCÜ
Abstract: Hydrophobins, found around the aerial hyphae and reproductive structures of fungi, are small amphipathic surfactant proteins. Due to these amphipathic properties, they can change the properties of the surfaces they are on. Thanks to these properties, hydrophilic surfaces can be obtained by imparting hydrophobic properties. However, the low production amounts in the studies conducted to date limit biotechnological studies. In the study, pPICZα-A vector was used for high protein expression, since its restriction maps, gene functions and intracellular replication mechanisms are known. P. pastoris was used for recombinant production due to its low amount of endogenous protein production, especially capable of performing posttranslational modifications. codon optimization was first performed after obtaining the gene sequence of the DewA protein from GenBank (Uniprot ID: P52750). Firstly, the secretion signal sequence region of this sequence and the stop codon of the gene were removed. Then, the cutting region of restriction enzymes (EcoRI and XbaI) that did not cut the resulting sequence was added to the sequence. After it was synthesized commercially, it was produced recombinantly by plasmid isolation, enzyme cutting reaction and ligation processes. It was then transferred to P.pastoris by electroporation method. Optimization of methanol induction was carried out at 0.5%, 1% and 1.5%. Protein isolation was performed by taking samples every 24 hours during the incubation. After protein isolation, the surface properties on teflon and glass surfaces were examined. The optimal culture condition for DewA expression was obtained at 1% methanol concentration as 77 mg/L in 96 hour. Recombinant DewA has been proven to change the surface characteristics on the teflon and glass surfaces. Conclusions: In the study, the DewA protein of A.nidulans was cloned into the pPICZα-A vector and recombinantly produced in the P.pastoris X-33 strain for the first time.
Keywords: HFBI Pichia pastoris silver staining, surface coating
Studying any course requires students to write a research paper at the end of their final term. When they get to the writing part, they face numerous difficulties like gathering the required information, establishing a good connection between ideas and thoughts, maintaining the flow of information, and so on.
Authors: S.Syafiq-Syauqi, M.Nur-Nazifah, M.Zudaidy Jaapar, M.Muhammad-Faiza
Abstract: This study focuses on pathogenicity and LD50 of Pangasius nasutus against Edwardsiella ictaluri. Pangasius nasutus or ‘patin buah’ is a native freshwater species of peninsular Malaysia and can be found in Pahang River. The market price for this species is high as it tastes better compared to other catfish. Edwardsiella ictaluri is a pathogenic bacteria and the causative agent that cause enteric septicemia of catfish (ESC) in the Pangasius species. However, the prevention measures against Edwardsiella ictaluri are still unknown for Pangasius nasutus due to the lack of research and study. Therefore, the objective of this study is to identify Edwardsiella ictaluri isolated from Pangasius nasutus cultured in Pahang River using polymerase chain reaction (PCR) and to determine the pathogenicity and LD50 of Pangasius nasutus against Edwardsiella ictaluri through injection method. There are four different concentrations of Edwardsiella ictaluri (1×1020 , 1×109 , 1×108 , 1×107 ) that were injected intraperitoneally including normal saline water as control to a total of 50 Pangasius nasutus. Observation of clinical signs and mortality were carried out for 30 days and LD50 was determined. The earliest clinical sign was observed at concentration of 1×1010 cfu/ml at 2 hours postinfection of Edwardsiella ictaluri where pale liver and congested kidney can be observed after dissection. Other clinical signs observed are inflammation on tail and fin, hemorrhagic fin, hemorrhagic upper mandible, discoloration, and inflammation on the lower part of body. The first mortality of Pangasius nasutus was at concentration 1×1010 cfu/ml at 2 hours of post infection. The highest cumulative mortality was recorded at concentration 1×1010 cfu/ml with 100% of mortality rate. From the result, the value of LD50 of Edwardsiella ictaluri calculated was 1×106 cfu/ml. From this study, it can be concluded that Edwardsiella ictaluri does affect the survivability Pangasius nasutus in Malaysia.
Keywords: Pangasius nasutus, Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, polymerase chain reaction (PCR)
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Authors: Abdullahi Ajao, Ganiyat Alasinrin
Abstract: This study investigated the spoilage patterns and biopreservation of tomato paste by lactic acid bacteria isolated from fermented milk products. All the isolates were screened for hydrogen peroxide, diacetyl, and lactic acid production. Isolate with the highest mean values of evaluating parameters was selected as protective culture for the biopreservation. The isolate was identified as Lactococus lactis strain Ganee-5 using molecular techniques, and the sequences were submitted to the Genbank Database to obtain the accession number (MH571417). Antimicrobial properties of the protective culture were evaluated against some selected spoilage bacteria E. coli (ATCC 25922), Listeria monocytogenes (ATCC 15313), Salmonella typhimurium (IFO 12529), and Staphylococcus aureus (ATCC 12600), varying zone of inhibitions ranged from 18-25 mm were detected. The potato paste was preserved with L. lactis culture, sodium benzoate and control samples while the control samples were left without preservatives. All the experimental set-up was left for 16 days. Physicochemical and nutritional analysis showed that tomato paste with L. lactis was preserved closely as much as sodium benzoate (p< 0.005). Therefore, L. lactis can be adopted for the preservation of the tomato paste to replace chemical preservatives.
Keywords: Protective culture, nutritional values, tomato paste, chemical preservatives, lactic acid, spoilage bacteria
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Authors: Suleyman Ilhan, Harika Atmaca
Abstract: The search for effective inhibitors against SARS-CoV-2, the virus responsible for the COVID-19 pandemic, has led to re-screening of existing potential molecules. Molecular docking and virtual screening techniques have been employed to identify potential drug candidates. Natural products, known for their wide variety and reduced toxicity, have gained significant attention in these screenings. Snake venom proteins, characterized by their diverse biological activities and unique molecular structures, offer a promising avenue for the discovery of new antiviral molecules. In this study, we focused on the investigation of snake venom proteins isolated from Echis carinatus, specifically Schistatin (SCH), Phospholipase A2 (PLA2), Disintegrin (DS), and Echistatin (ECH) for their potential as inhibitors against SARS-CoV-2. Through molecular docking analysis, the binding interactions between these venom proteins and key SARS-CoV-2 targets, the main protease (Mpro), and the ACE2 receptor were examined. Results revealed that PLA2 exhibited the most favorable binding affinity to both Mpro and ACE2, surpassing the reference drug ritonavir (RTV). SCH, DS, and ECH also demonstrated promising binding affinities with both targets. This study sheds light on the unexplored potential of snake venom proteins, specifically PLA2, SCH, DS, and ECH from E. carinatus venom, as inhibitors against SARS-CoV-2. The exploration of snake venom proteins presents an intriguing avenue for the discovery of novel drug candidates with broad applications in the treatment of various diseases, including viral infections such as COVID-19.
Keywords: Venomous snakes, therapeutic toxins, protein interactions, molecular docking
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Authors: Merve Sönmez, Ferah Cömert Önder, Esra Tokay, Ayhan Çelik, Feray Köçkar, Mehmet Ay
Abstract: Natural products have a key role for drug discovery in pharmacology and medicine. Prunus spinosa L. (blackthorn) grown in Çanakkale province in western Turkey, is known as a medicinal plant, a rich source of biologically active compounds such as phenolics, flavonoids and anthocyanidins. The flower and fruit extracts of the plant are subjects of many studies, but they usually lack details of its potential for bio-inhibition studies. Thus, this study aimed to investigate the antioxidant, enzyme inhibition and antiproliferative activity studies of the methanol, ethyl acetate, dichloromethane, and n-hexane extracts of the plant. The ethyl acetate and methanol extracts showed better antioxidant activity with DPPH, FRAP, CUPRAC, and TEAC assays. Enzyme inhibition studies of the extracts were performed using β-lactamase, proteases and tyrosinase. The methanol (FL) and ethyl acetate (FL and L) extracts at the concentration of 10 mg/mL, displayed better inhibition against α-chymotrypsin, trypsin, and papain with values of 22.6%, 34.7% and 92.1%, respectively. Furthermore, the methanol and ethyl acetate extracts have displayed higher cytotoxic effect against cancer cells such as Hep3B and HT29 compared to healthy cells (HUVEC) using MTT assay. The findings suggest that P. spinosa L. extracts and their components may be potential for further investigations of novel drug candidates from natural sources.
Keywords: Prunus spinosa L., enzyme inhibition, proteases, cytotoxicity, antioxidant
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If you’re someone who has been gazing at a blank page on your screen and is not quite sure how to approach writing your research paper, then this is the article for you.
Read more:- www.ardaconference.com/blog/how-to-write-thesis-for-resea...
Authors: N Amira-Syahidah, M Nur-Nazifah, R Rimatulhana, AA Sabuti, Mohamad Shafiq Mohd Ibrahim
Abstract: Striped catfish, Pangasianodon hypophthalmus is a native species to most Asian countries, including Malaysia which faced series of bacterial disease such as edwardsiellosis caused by E.ictaluri. Edwardsiella ictaluri is a Gram-negative intracellular bacterial pathogen which had caused mortalities in farmed or wild fish in lots of countries and accountable for large economic losses. This study was conducted to determine disease status of Pangasius spp due to Edwardsiella ictaluri and to determine the risk factors of E.ictaluri in striped catfish in Pahang River, Malaysia. Four sampling sites were chosen, two sites in Pekan (Kampung Belimbing and Kampung Tanjung Pulai) and two sites in Temerloh (Kampung Teluk Ira and Kampung Bintang). Thirty samples were collected from each farm for 6 months consecutively and four organ tissues were taken which were liver, spleen, kidney, and brain. External and internal clinical signs were recorded accordingly. DNA was extracted from all tissues and pursued for PCR to detect the presence of Edwardsiella ictaluri. Results show that the infected fish has gross internal clinical signs such as patchy liver, white nodular spleen, and congested kidney. The prevalence rate of E.ictaluri was highest at Kampung Tanjung Pulai in June with 16.67% compared to all farms. No infection of E.ictaluri shown at Kampung Belimbing during the whole sampling session. Spearman’s rho correlation showed that the prevalence of E.ictaluri has strong correlation with temperature in Kampung Tanjung Pulai (p > 0.05). Meanwhile those bacteria prevalence have strong relationship with ammonia, sulfide and total suspended solid (TSS) with p< 0.05. The susceptible size for this bacterium in striped catfish is between 1 to 50g. To our knowledge, this paper is the first report for Edwardsiella ictaluriin cage cultured P.hypopthalmus in Pahang River, Malaysia.
Keywords: Prevalence, Edwardsiella ictaluri, Pangasius hypophthalmus, risk factor, PCR
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Authors: Murat Keser, Harika Atmaca, Saziye Burcak Karaca
Abstract: Triple-negative breast cancer (TNBC) is recognized as a challenging subtype due to its poor prognosis. Recent molecular profiling studies have unveiled a significant subset expressing the androgen receptor (AR) subset which may respond to AR-blocking agents, offering a potentially effective treatment strategy. This study aims to investigate the potential synergistic cytotoxic and apoptotic effects of the AR antagonist enzalutamide (ENZA) in combination with CDK4/6 inhibitors palbociclib (PB) or ribociclib (RB) and compare the effectiveness of these combinations in TNBC cells. Results revealed that ENZA in combination with PB or RB induced synergistic cytotoxic cytotoxicity in all tested TNBC cell lines. While synergistic cytotoxic combinations of ENZA with PB did not induce apoptosis in any TNBC cell line, ENZA+RB combinations exhibited a synergistic apoptotic effect. This study suggests the ENZA+RB combination may be more favorable due to its apoptosis-inducing effect. However, these data need to be further supported by detailed in vivo and clinical studies.
Keywords: Triple-negative breast cancer, enzalutamide, palbociclib, ribociclib
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Read through An APA Format Example Shows Ways To Compose A Research Paper to understand the essentials of APA research paper writing through some excellent examples. The templates show how you can format your paper correctly with the rulebook of the APA referencing style, so you can structure a better research paper while keeping the format intact.
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