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Entry in category 4. ©Sabine Fièvre; See also bit.ly/snsf_comp_copy
The present video was obtained after confocal microscopic acquisition of cortical neurons from adult mouse brain. Neurons from the same cortical layer (in blue) have been in utero electroporated with a fluorescent protein: GFP. One of these neurons (in pink) has been filled during 30 minutes with biocytin present in a patch clamp glass pipette. The biocytin was conjugated with streptavidine fused with a fluorescent protein (Alexa 405), highlighting the complex morphology of the neuron.
The microscopic acquisition was done with a 60x optical zoom - confocal microscope: 100 stacks of 1 µm thickness were taken, allowing the 3D reconstruction of the biocytin filled neuron (in gold) with the software Imaris (filament tool). In this movie you can observe the neuron rotating around the axis of its apical dendrite.
Esther Klingler and Sabine Fièvre have equally contributed to the creation of this picture from the design of the scientific experiment to the treatment of the final image.
I have mounted this video from high-resolution microscopic images of neurons in the mouse brain cortex." First, in blue appear neurons marked at the moment of their birth during embryonic development. One of these neurons has been filled in adulthood with a fluorescent marker, visible here in pink, revealing its complex morphology.This three-dimensional computer reconstruction has made it possible to highlight this remarkable morphology , Represented here in the form of golden filaments, the neuron then turning around its main axis, allowing itself to be appreciated beneath its various facets.
Esther Klingler and Sabine Fièvre contributed equally to the development of this image, from the experimental design to the final processing of the image. ¦ Image#4_14
22/05/2008
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Aha! Well I've finally had a wee mess around with THE cam and revisited an old subject. (though i've decided it will be revisted again when my concentration is higher and when i've made an effort to dust and polish the bulb - it was so speckly that i think i've ruined a fair amount of the detail trying to de-speckle in ps. sigh!)
Anyways... because i've got that new cam burning my fingers waiting for inspiration to strike - here's me first of many. :-)
Close-up mosaic of the big active region NOAA 2192, at 3 meter of focal
Take a look also at my astronomy video channel.
Beautiful detail when Viewed On Black
I took seven shots at slightly different focal points of the inside of the trumpet of a daffodil, and used CombineZP ( a free software tool) to stack all seven images to create the final image.
Once the stack was complete, I selected the centre filaments and on a separate layer sharpened them with a high pass filter at 60% opacity, and then in a new layer, inverted the selected area and ran NR on it, before a soft NR over the flattened image.
Finally, after adding the drop shadow border, I ran two passes of USM after reducing the image size for the web.
C&C as always.
Viewable Exif:
Camera Maker: Canon
Camera Model: Canon EOS 450D
Image Date: 2010:04:20 22:09:08
Caption: Modified by CombineZP
Full Exif:
Camera Maker: Canon
Camera Model: Canon EOS 450D
Lens: 105mm
Image Date: 2010-04-20 20:34:51 +0100
Focal Length: 105.0mm
Aperture: f/7.1
Exposure Time: 0.0063 s (1/160)
ISO equiv: 100
Exposure Bias: none
Metering Mode: Spot
Exposure: Manual
Exposure Mode: Manual
White Balance: Auto
Flash Fired: Yes (Manual)
Color Space: sRGB
Photographer: Copyright A View of the UK - Paul Iddon Photography
Petite balade dans un parc à Hyères (Var, France). La pluie ne nous a pas empêché de pouvoir admirer la diversité de fleurs, dont cette fleur à la couleur rouge bien pétant et ses pétales en formes de "pics".
The rain didn't stop us to admire the flowers diversity in a garden in hyères (South of France). Thsi flower was very peculiar as its color was a vivid red and its petals like peaks.
In the courtyard at the Victoria and Albert Museum. www.vam.ac.uk/shop/whatson/index/view/id/1125/event/Elytr...
Filament 2007
17"x18"x19"
Ceramic and acrylic
Brief Bio:
Tyler Lotz received his MFA from the New York State College of Ceramics at Alfred University in 2000. His ceramic sculptures and vessels have been shown in solo and group exhibitions in the United States and abroad. Tyler’s work has been acquired by significant private and institutional collections. He also has been a resident artist at the Archie Bray Foundation in Helena, Montana. Residing in central Illinois, Tyler is currently an Assistant Professor in The School of Art at Illinois State University.
www.cfa.ilstu.edu/art/faculty_staff/biographydetail.asp?u...