View allAll Photos Tagged bootstrapped
Tech Cocktail and Refresh Miami hosted a Holiday Party and All-Star awards in Miami. Attendees mingled, saw drinks demoed and tried them, ate some holiday treats and spread all holiday cheer.
We also presented the 2014 All-Star Awards that the Miami community nominated and voted for. Congratulations to the winners and thanks for making 2014 a great year! To read more about the event, visit this: bit.ly/1wD4Pk3
Best Community Leader - Matt Haggman
Best Big Company with Startup Culture - dotCo
Best Social Good Startup - Ignition Fund
Most Likely to Get Acquired - Learner Nation
Most Active in Local Community - LAB Miami
Best Company Culture - Symptify and Wyncode
Most Charismatic/Best Founder(s)/Leaders - Mitali Saxena
Biggest Pivot - Rokk3r Labs
Most Disruptive - Bitstop
Most Innovative Product and Best Design - We Are Visual
Best Bootstrapped - Fashom
Photographer Credit: Noir 85
There is a strange link between economics and nature. When the first European settlers encountered the Great Plains, they referred too much of the land as the Great American Desert. Despite their concerns they set about plowing up the fields under the theory that the rain follows the plow. The genesis of this theory seemed to be rooted in the concept that somehow planting trees and crops would raise the amount of moisture in the air and the semi- arid landscape would, in time, receive enough rain to sustain farms. They were wrong. The plow follows the rain.
I often wonder about the link between housing, population and business. Like the 19th century pioneers, I wonder if business follows the houses. Or, is it the other way around? This is an important question as the city looks to rebuild. Does the city place an emphasis on building clearing land and pushing new housing development, or should most of the energy be placed on creating jobs? Perhaps the question is answered best by considering what economic force has created this landscape of abandonment. Without getting into the myriad of reasons why, and all of the political and cultural noise that discussion brings, let’s state for the record that the single biggest force driving the decline of Detroit has been the flight of the middle- class; first middle- class whites and now middle- class blacks. What has been left behind is a bewildering level of poverty and lack of resources. No sense talking about bootstrapping to folks who can’t afford shoes. So I guess the question we should be asking is how we get middle- class families back into the city. Business will follow the house.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
Holiday parties in January make a lot of sense if you think about it... :)
Founded and initially bootstrapped by twin brothers Lucas and Lee Brown, TUNE is the Seattle-based attribution analytics company behind the products MobileAppTracking (MAT), HasOffers, and MobileDevHQ (MDHQ). With a mission to make mobile marketing better for everyone, TUNE creates Software-as-a-Service (SaaS) products to help marketers manage performance advertising relationships across mobile and desktop. Named the 63rd fastest growing company by Inc. 500 in 2013 and a top 10 mobile advertising company by VentureBeat, TUNE is backed by Accel Partners (investment led by Rich Wong) and has more than 250 employees with additional offices in San Francisco, New York, Berlin, Seoul, Tel Aviv, and London.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
While I had a good idea of how I wanted to design the body for Smart Doll Girl, I've never had my fancy tickled by a guys body which is why I'm asking for your feedback.
If I listened to absolutely everybody however, he will probably end up with ten arms and a beer belly so I will vet the feedback and make the final decision.
And for those who have not already done so - check out the Smart Doll website to see how I bootstrapped the Smart Doll business.
View more at www.dannychoo.com/en/post/27204/Smart+Doll+Guy.html
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Macintosh Color Classic running Mac System 7.1 Update 3.0. This machine has the Apple IIe PDS card installed and a Apple 5.25" floppy drive and joystick attached.
Apple IIe Card now in control, bootstrapped from the Apple 5.25" floppy drive having finished loading from the System Disk 3.1 and exited to BASIC.
Greetings to Reddit r/retrobattlestations!
When: Thursday, October 7th, 6:30-9:30pm
Where: NYU Stern Kaufman Management Center 44 West 4th St., Rm 2-60
Panelists: Anthony Casalena (Squarespace), Anthony Volodkin (The Hype Machine), Danny Wen (Harvest), Spencer Fry (Carbonmade).
This event was in partnership with UltraLight Startups.
Panelists shared candid, real-life stories of bootstrapping their technologies. Each entrepreneur founded their company with zero external capital and now has over $1 million in revenues.
Photo by @matylda
When: Thursday, October 7th, 6:30-9:30pm
Where: NYU Stern Kaufman Management Center 44 West 4th St., Rm 2-60
Panelists: Anthony Casalena (Squarespace), Anthony Volodkin (The Hype Machine), Danny Wen (Harvest), Spencer Fry (Carbonmade).
This event was in partnership with UltraLight Startups.
Panelists shared candid, real-life stories of bootstrapping their technologies. Each entrepreneur founded their company with zero external capital and now has over $1 million in revenues.
Photo by @matylda
Sachin dev duggal businessman behind Builder.ai, a human-assisted AI with arranging that makes building and running programming as basic as mentioning pizza. Since 2012, he's bootstrapped Builder.ai to help anyone with making thought, without knowing a single line of code – and without lounging around inactively, money or resources. He has a B.Eng degree from Imperial College London, and was Deutsche Bank's most energetic delegate ever.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Program director Catherine Rohr (on extreme right) helps a group of active volunteers evaluate the performance of the competitors as individuals and as teams. At several points the group met with the various teams to chat and offer feedback.
As part of Defy Venture's first Mocktail competition, the teams, comprising ex-felons seeking to start businesses in New York City, created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Designed by Byriah Loper
Folded by me
This one was trickiest at the start because the first frame wouldn't support itself until it was fully constructed, presenting an interesting "bootstrapping" problem.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
Teams of ex-felons seeking to start businesses in New York City compete in Defy Venture's first Mocktail competition. The teams created and prepared nonalcoholic cocktails (mock cocktails) that they then sold to guests. The drinks were as colorful as they were refreshing, especially in the midst of a historic heatwave.
As participants in the MBA-style Defy program, they learn business, accounting, and sales skills that can lead to successful ventures and careers.
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
Bootstrapped Brews is a celebration of the things that make Colorado the state it is today: entrepreneurship, beer and charity.
Bootstrapped Brews, now in its second year, is the annual members’ conference (21 & up) of the Bootstrapped Brews Association (BBA). It is an opportunity to connect with like-minded Colorado-area startups, to secure bragging rights for making some of the finest homebrew in town, and to help an amazing cause with 100% of proceeds benefiting Promise Ranch. It is part networking event, part competition, and completely fun. We welcome any Colorado-based company with a startup mentality and a passion for brewing.
How it works
Participating startups will be given a space and a table to set up any promotional materials they like. Additionally, startups will be provided with a bucket (for kegs or bottles) and ice to keep their beer cold. In return, each startup must brew and provide at least 5 gallons of beer by August 1st, 2015 (1 week prior to the event for judging purposes). Startups, you are also responsible for serving your beer at the event.
The bulk of the event will be unstructured, so participants can mill around, visit with fellow startups, and drink plenty of beer. Around 8:00 pm, a panel of judges will share their picks for the best brews (first, second, and third place), as well as People’s Choice, and awards will be handed out.
Photography by 23rdstudios www.23rdstudios.com Please contact for permissions info@23rdstudios.com
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
Westercon, 2 July 2007. Awesome panel; thanks to Kathryn Myronuk and Brad Templeton for doing the bulk of the work, and Eliezer Yudkowsky, for keeping us honest. :)
Here's the promised reading list, courtesy Max Rible Kaehn:
Charles Stross, Accelerando and sequel Glasshouse, Singularity Sky and sequel Iron Sunrise. You can read Accelerando online at www.accelerando.org/ ; Charlie's web pages and blog are at www.antipope.org/ .
Cory Doctorow, Down and Out in the Magic Kingdom, is set in a post-scarcity future where the only currency is reputation. His web site is www.craphound.com/ , and you can read almost all his work there.
Vernor Vinge: The Peace War and sequel Marooned in Realtime, collected as Across Realtime; A Fire Upon the Deep and prequel A Deepness in the Sky. Rainbows End is a near-future look at advancing technology that is on the road to the kind of intelligence enhancement that will lead to a Singularity.
Alastair Reynolds: Revelation Space, Redemption Ark, Absolution Gap. Chasm City and Diamond Dogs, Turquoise Days are set in the same universe.
Richard Morgan: the Takeshi Kovacs books Altered Carbon, Broken Angels, and Woken Furies are set in a future where personalities can be transferred and copied. David Brin's Kiln People has a different take on that theme, as does one of the movies on the Hugo nominees for this year: www.locusmag.com/SFAwards/Db/Hugo2007.html
Greg Egan: Permutation City, Diaspora.
Peter Watts: Blindsight is the tale of sending a group of enhanced humans-- who have all made some serious tradeoffs for their enhancements-- to contact visiting aliens. A chilling and excellent read.
Ken MacLeod: Newton's Wake is set after the Singularity; the Fall Revolution sequence, The Star Fraction, The Stone Canal, and The Cassini Division, along with The Sky Road (an alternate future based on a different decision taken by a character in The Star Fraction) span it.
Bruce Sterling has a number of books looking at our near future; Holy Fire was mentioned in the panel.
Robert Charles Wilson: Darwinia, Blind Lake.
Thomas T. Thomas: Crygender.
Thomas J. Ryan: The Adolescence of P-1, is a view of a bootstrapping AI that gets its start on 1970s-era mainframes.
Nonfiction:
Ray Kurzweil: The Singularity is Near
Douglas Hofstadter: I am a Strange Loop
The Singularity Institute for Artificial Intelligence -- www.singinst.org/ -- want to make sure that the first superhuman intelligence is wholly beneficial to humankind.
Openomy: self-funding/bootstrapping?
"... Openomy is completely free for now. In the future there will be an additional premium membership, in exchange for some nice extras. ..."
Is there any reason why you are not charging something? Sure you need free accounts to let people try the service. What about a Pro-Version like flickr. [0]
A question I'd like to ask is:
Q What nice extras do you offer?
For me nice extras would be:
- tools specifically to post to twitter (simple twit poster) , archive the post, process meta data (who replied, when)
- tool(s) specifically to post images to flickr (simple flickr uploader), archive image, text, tags & who replied.
- rss feed back of flickr + twitter archive that I can customise (select twit + flickr images mixed, only twitter, only flickr, only flickr, only flickr images by tag etc)
- allow my friends to subscribe to my feed
Abstraction above current service
This is an abstraction above your current service. But is readily consumable. The real problem is being able to find what people want, then charge for it. Flickr built this kind of back-end then built the photo site on top of it. Is this what you need? A purpose built public site on top of you data service?
"... Over the past couple of years, as we predicted, the world is moving to an almost entirely web-based software model. We post our photos to Flickr, write our documents on Zoho, etc. Unfortunately, it's much too difficult for us to use our photos posted on Flickr within our documents on Zoho. ..."
My view is you are doing a great job of creating the basic building blocks to achieve this. What I'd want to see is a pay for use service that allows you to demonstrate these aims. [2]
Turning commodity service into something users want
Your service utilises commodity bandwidth and storage. If you look at how flickr utilised these same commodities by harnessing the explosive use of cameras I'm sure you can see a way to make money from it. How flickr balanced the back-end development (infrastructure) with the front-end (consumer facing) is a case study in itself. This means more development, but that's what it's about right?
Reference
[0] The free version of flickr allows limited usage. It does not stop you upload images, but the cost for use is worth it if you intend to use it over a longer period.
[1] Caterina Fake, ITConversations, "The History of Flickr", www.itconversations.com/shows/detail1755.html
[2] One area I don't see is how you can share your data with friends, like friendfeed does so well.
next >>>
Circular representation of a phylogenetic tree I used in my thesis. Wisely the names are omitted, just in case some further analysis changes the topology…
Bootstrapped Brews is a celebration of the things that make Colorado the state it is today: entrepreneurship, beer and charity.
Bootstrapped Brews, now in its second year, is the annual members’ conference (21 & up) of the Bootstrapped Brews Association (BBA). It is an opportunity to connect with like-minded Colorado-area startups, to secure bragging rights for making some of the finest homebrew in town, and to help an amazing cause with 100% of proceeds benefiting Promise Ranch. It is part networking event, part competition, and completely fun. We welcome any Colorado-based company with a startup mentality and a passion for brewing.
How it works
Participating startups will be given a space and a table to set up any promotional materials they like. Additionally, startups will be provided with a bucket (for kegs or bottles) and ice to keep their beer cold. In return, each startup must brew and provide at least 5 gallons of beer by August 1st, 2015 (1 week prior to the event for judging purposes). Startups, you are also responsible for serving your beer at the event.
The bulk of the event will be unstructured, so participants can mill around, visit with fellow startups, and drink plenty of beer. Around 8:00 pm, a panel of judges will share their picks for the best brews (first, second, and third place), as well as People’s Choice, and awards will be handed out.
Photography by 23rdstudios www.23rdstudios.com Please contact for permissions info@23rdstudios.com
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
Tech Cocktail and Refresh Miami hosted a Holiday Party and All-Star awards in Miami. Attendees mingled, saw drinks demoed and tried them, ate some holiday treats and spread all holiday cheer.
We also presented the 2014 All-Star Awards that the Miami community nominated and voted for. Congratulations to the winners and thanks for making 2014 a great year! To read more about the event, visit this: bit.ly/1wD4Pk3
Best Community Leader - Matt Haggman
Best Big Company with Startup Culture - dotCo
Best Social Good Startup - Ignition Fund
Most Likely to Get Acquired - Learner Nation
Most Active in Local Community - LAB Miami
Best Company Culture - Symptify and Wyncode
Most Charismatic/Best Founder(s)/Leaders - Mitali Saxena
Biggest Pivot - Rokk3r Labs
Most Disruptive - Bitstop
Most Innovative Product and Best Design - We Are Visual
Best Bootstrapped - Fashom
Photographer Credit: Noir 85
Effect of the Hxt on the transcription of other Fusarium oxysporum sugar transporter genes during consolidated bioprocessing (CBP) of wheat straw/bran.Wild type fungus 11C, Hxt-silenced mutant pSilent-1-Hxt-3 and overexpression mutant pBARGPE1-Hxt-6 were aerobically cultured on wheat straw/bran (10?1 ratio) and RT-PCR was conducted using RNA isolated from samples harvested 24 h post-inoculation. (A) Transcript accumulation in RNA extracts was quantified relative to that of the housekeeping gene ?-tubulin (FOXG_06228.2) by 2?-??Ct method, where ??Ct?=?(Ct, Target gene - Ct, ?-tubulin). Results are based on two experiments, each with three replicates per strain/mutant per medium. Bars indicate the SEM (LSD0.05?=?0.015) (B) A phylogenetic tree was constructed based on the protein sequence of 20 F. oxysporum strain 4287 sugar transporters. Protein sequences were aligned using European Bioinformatics Institutes?s ClustalW2 tool (www.ebi.ac.uk) [62] and a distance tree of 100 bootstrapped data sets was generated by using the Phylogeny.fr program and the neighbor-joining method [64]. Arrows indicated the three genes alluded to in part (A) above that were affected by Hxt expression.
Holiday parties in January make a lot of sense if you think about it... :)
Founded and initially bootstrapped by twin brothers Lucas and Lee Brown, TUNE is the Seattle-based attribution analytics company behind the products MobileAppTracking (MAT), HasOffers, and MobileDevHQ (MDHQ). With a mission to make mobile marketing better for everyone, TUNE creates Software-as-a-Service (SaaS) products to help marketers manage performance advertising relationships across mobile and desktop. Named the 63rd fastest growing company by Inc. 500 in 2013 and a top 10 mobile advertising company by VentureBeat, TUNE is backed by Accel Partners (investment led by Rich Wong) and has more than 250 employees with additional offices in San Francisco, New York, Berlin, Seoul, Tel Aviv, and London.
Holiday parties in January make a lot of sense if you think about it... :)
Founded and initially bootstrapped by twin brothers Lucas and Lee Brown, TUNE is the Seattle-based attribution analytics company behind the products MobileAppTracking (MAT), HasOffers, and MobileDevHQ (MDHQ). With a mission to make mobile marketing better for everyone, TUNE creates Software-as-a-Service (SaaS) products to help marketers manage performance advertising relationships across mobile and desktop. Named the 63rd fastest growing company by Inc. 500 in 2013 and a top 10 mobile advertising company by VentureBeat, TUNE is backed by Accel Partners (investment led by Rich Wong) and has more than 250 employees with additional offices in San Francisco, New York, Berlin, Seoul, Tel Aviv, and London.
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
This is the first workbench I made. The parts for the legs for the larger main build table are pre cut and stacked on top. This smaller table was built to the size of the pre-cut 2'x4' plywood sheets that Home Depot carries. I didn't have a circ saw or anything else to cut a larger table top.
This project was for bootstrapping the workshop. So there was basically nothing in there. I had to rent a miter saw from Home Depot to get the tables done.
"The best way to predict our future is to invent it."
(Alan Kay 1971)
Open Sailing aims to design and invent future lifestyles to overcome any possible natural and manmade disasters stimulating people’s ingenuity and sense of solidarity. Might it be global warming or energy conflicts, we are living in a time where we are sniffing the ‘Apocalypse’, finally realising our human part of responsibility as the earth is crumbling. 2012 is a year when a collection of apocalyptic events are rumored to happen. We are taking 2012 as an ideal dystopic symbol we design for. 2012 is tomorrow, we must design quickly using these constraints and invent bootstrapping DIY technologies.
Open Sailing method is to convert apocalyptic threats into design constraints. From our compiled set of threat maps, we found that oceans are the safest locations. Ocean survival architecture became our new starting point, but we need to go further than surviving : how can we live together in this new fluid configuration and remain a hyper-connected intelligent social being? We are trying to make a truly “open architecture” : pre-broken, under-defined, reconfigurable, moveable, pluggable, organic, fluid. Can we reach a harmonious dynamic state of interdependence with each other and the earth? Is this the next step of civilization progress? Will we dissociate our concept of progress with infrastructure and metropolis?
NEXT STEPS IN 2009
Finding motivated knowledgeable collaborators and funders (february).
Prototyping technology equipment for ocean living, UK (march ~ april).
Testing the Open Sailing in the Atlantic ocean, Morocco (may).
Public presentation of Open Sailing researches (june)
Model made by
Martin gautron : martingautron.com
Hiromi Ozaki : hiromiozaki.com
Adrien Lecuru :
Cesar Harada : cesarharada.com
Photography direction, Cereinyn Ord : cereinyn.com/
This is what I'm drinking this morning... 1st place in the Rwanda Golden Cup competition (a precursor to future Cup of Excellence events) bought at auction and roasted by Intelligentsia. Amazing to think that a coffee of this beauty and quality would have been unimaginable just a few short years ago but Rwanda is rapidly bootstrapping into being a top tier producer of specialty coffee.