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The new alignment at Heamies Farm, with Freightliner Class 66/5 No. 66506 'Crewe Regeneration' leading 4M88 0932 Felixstowe North - Crewe Basford Hall off the new alignment of the down slow from Searchlight Lane Junction on 17th August 2016. Previously a four-track formation continued around the curve to Norton Bridge, which now with closer inspection revealing that the now redundant fourth track (down slow-above red third container) has been lifted. Copyright Photograph John Whitehouse - all rights reserved
Sequence alignment and phylogenetic analysis of SKD1 proteins from Zea Mays and other species.A An alignment is shown for the deduced amino acid sequence of SKD1s from Zea Mays (Zm), Arabidopsis thaliana (At), Lycopersicon esculentum (Le), Oryza sativa (Os), Brachypodium distachyon (Bd), Hordeum vulgare (Hv) and Saccharomyces cerevisiae (Sc). The numbers on the left indicate the amino acid position. Identical residues in all these proteins are shown in a black background. Dashes indicated gaps introduced for optimal alignment. The putative MIT and AAA domains are underlined with a thick red line and a thick green line, respectively. B Phylogenetic tree based on SKD1 protein sequences from yeast, plants, and animals. The bootstrap values shown were calculated based on 500 replications. The tree was constructed using the neighbor-joining method. Z.mays, AY105155; A.thaliana, At2g27600; L.esculentum, AK324437; O.sativa, AF499028; B.distachyon, LOC100837561; H.vulgare, AK359160; D.melanogaster, NP_573258; G.gallus, AJ720732; H.sapiens, AF038960; M.musculus, NP_033216; S.cerevisiae, NP_015499.
Sequence analysis of the PDIR non-catalytic domain.(A) Occurrence of the domain in protein disulfide isomerases and other proteins. Human ERp57 is shown for comparison. Catalytic motifs are shown in catalytically-active thioredoxin-like domains. (B) Rooted phylogenetic tree of proteins shown in panel (A). Sequences labeled WUBG_02370 and RNA methyltransferase are proteins from parasitic nematodes Wuchereria bancrofti (EJW86719) and Brugia malayi (XP_001896925); mosquito PDIR is from Aedes aegypti (XP_001659136). The N-terminal catalytic domain of ERp57 was used for the phylogenetic tree. The figure was generated with ClustalW [29] and TreeViewPPC [30]. (C) Sequence alignment of the non-catalytic domain from PDIR proteins from human (NP_006801), rabbit (XP_002716857), rattlesnake (AFJ50881), chicken (XP_422097), zebrafish (XP_001107048), frog (XP_001086600), fly (XP_609645), and sea urchin (XP_001200801) and the related sequence from Brugia malayi RNA methyltransferase (XP_001896925). The consensus sequence is shown below; the secondary structure elements are above the sequence.
The magnets do an amazing job keeping the TouchFire aligned.
This photo shows something else: silicone rubber grabs dust and hair. You can run it under water to clean that stuff off.
Read the full review here:
www.suntimes.com/technology/ihnatko/13715183-452/touchfir...
I made these this past week as part of my new head tube alignment system. On the left, an adjustible jack. On the right, a tube centering gauge. (The gauge itself I bought from Joel of Clockwork bikes; I built the base. Eventually I'll make a new gauge that's a little more to my liking.)
Name: Bontar
Title: Toa of Stone, Ruler of Po-Magna
Alignment: Lawful Good
Location: Po-Magna
Weapons: Twin Saw-Tooth blades
Lore: Most of the 6 original Toa, such as Frizzak, serve as guardians to their respective region. Bontar however was seen as more. A steely individual with calm judgement and modesty, Bontar was placed in defensive of the Po-Magna region – an area which was, until his arrival, the stronghold of the Bohrok. With the help of the Matoran, and a fellow Toa of Earth, he laid siege on the stronghold, reclaiming the region as the first of many areas of the island to be ridden of Bohrok. However, in the siege his fellow Toa of Earth fell, due in part to Bontar’s hot-bloodedness and lack of emotional control. From that day forth, he vowed to never lose control again; his judgement became sound, and his Matoran hailed him as Ruler of Po-Magna. However, whilst composed in ruling and combat, he sometimes finds his old primal self grasping for control – something he only gives into when he knows he has no choice. Many pronounce his combat technique as an art form, second only to Frizzak’s – despite wielding such brutal weapons, he executes strikes with precision so great, it is said he can remove an opponent’s mask in one swipe, leaving no mark on the opponent themselves – except, that is, for their shame.
Sequence and expression analysis of GsWRKY20.(a) Amino acid sequence alignment of WRKY domains among GsWRKY20 and other type Ⅲ WRKY TFs, Sequences were aligned using ClustalW, and gaps were introduced to maximize alignment, filled triangle marks the cystine and histidine in the C2HC-type zinc finger domain.(b) The phylogenetic tree of the WRKY TFs. The phylogenetic tree was constructed using MEGA 4.1. Total 24 WRKY proteins from Oryza, Arabidopsis, Gossypium, Medicago, Triticum aestivum, Glycine max, Thlaspi caerulescens and Solanum were selected to construct the phylogenetic tree.(c) Tissue-specific expression analysis of GsWRKY20 by real-time quantitative PCR (qRT-PCR). Tissues included trifoliate leaf (TL), root (RO), stem tip (ST), flower bud (FB), and pod (PD). Expression of GAPDH was used as an internal control. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.(d) qRT-PCR analysis of GsWRKY20 diurnal expression under SD and LD. Trifoliate leaves were sampled every 4 h at 21 DAE. White and black bars at the top represent light and dark phases, respectively. Relative transcript levels were analyzed by qRT-PCR and normalized by GAPDH. The experiment included three fully independent biological repeats, and three technical repeats and the mean value is shown.
this is a panorama made from 8 pictures.
It was taken on May, the 1rst, at 3:00 AM GMT.
At this moment, Venus and Jupiter were in conjunction. The milky-way core was still visible.
Location : La Seyne sur Mer, near Toulon, France.
Setup : Nikon Z6 II + Nikkor 24/70 @ 24 mm -f/4 - ISO 1600 - 20 s
River Dargle Flood Defence Scheme.
These images were taken during the first week of July, 2017.
The levels of activity fluctuate along this stretch of the riverbank, and especially around the Irish Rail bridge, informed by the tides and priorities.
Since Summer 2016, this is where the heavy-duty engineering works have been taking place.
This is a section of the flood protection scheme that I have not covered in detail -- it's inconvenient for me to access, and others cover it much better.
Standing below an access bridge, adjacent to the Bray Boxing Club (from whence sprang Katie Taylor, and others of illustrious note), looking back up the river, towards the town direction.
In the foreground is the Railway bridge, and in the distance we can see some construction works taking place on the Ravenswell Road, temporarily closed due to on-going works.
That was the site of the old Bray Golf Club -- hotly contested as a (potentially) poorly considered site for a shopping centre development complex, and still an area of ground that has to act as a flood plain in the event of tidal surges.
As well as raising a heightened flood protection wall, they've created an access ramp down to the riverside.
The area in the background, site of the old Bray Golf course, was both a works compound and vehicle route for the transportation of material to/from the other sectors along the River Dargle involved in construction works.
The Irish Rail Bridge, Bray Harbour:
Phase 1 flood defence works to the Irish Rail bridge commenced in August 2016.
Phase 2 flood defence works will be completed during May to September 2017. This work is being undertaken directly by Irish Rail.
The work includes strengthening the integrity of the bridge by creating buttresses around the base of each pillar.
To do this they have to pile-drive sheets into the river bedrock.
The work is complicated by;
(a) the need not to damage or disturb in any way the actual bridge itself (Irish Rail train and DART carriages pass overhead on an hourly basis), (b) the confined spaces under the bridge, and (c) the twice-daily rising tides from Bray Harbour which spill upriver into the newly expanded basin.
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Bray Harbour / Irish Rail Bridge:
The harbour was constructed between the years of 1891 and 1895.
A railway bridge was first built here in 1853.
The following year saw the opening of a railway line between Dublin and Bray.
It was also known as the 'Harcourt Street Line'
Famed Irish engineer William Dargan, known affectionately as ‘the father of the Irish Railways’, was the man involved with bringing the rail to Bray, and in it's construction, and which moulded the town into a fashionable resort.
Dargan designed and built Ireland's first railway line from Dublin to Dún Laoghaire in 1833.
Mid-18th century plans proposed the building of a 12.5-mile (20 km) railway from Bray Daly, which opened on 10 July 1854 to initially terminate at Harcourt Road. (Harcourt Street Station was not built until 1859).
The building of the line was done by two railway companies: The Dublin, Wicklow and Wexford Railway (DW&WR), who built the line from Dundrum to Bray and the Dublin, Dundrum and Rathfarnham Railway (DD&RR), who were to build the line from Harcourt Street to Dundrum.
The latter failed to do so, and the 'Dublin & Wicklow Railway' took over the line works.
Following the Beddy Report of 1957, CIÉ began to realise that in an effort to save money, all the non-profitable rural railway branch lines would have to close. The Harcourt Street line was one of these unprofitable railway lines and so the decision to close the line was ultimately reached.
In October 1958, CIÉ gave notice of the closure in the local papers.
The last train, CIÉ 2600 Class AEC railcar number 2652, left Harcourt Street at 4:25pm on 31 December 1958.
The route was preserved after closure, allowing for a potential future reopening. The route corridor remained mostly in-situ until the 2000s. The section between the Grand Canal crossing and the old Stillorgan station was chosen for use by the Luas light rail system whose Green Line opened in 2004. The line crosses Dundrum on the new William Dargan cable-stayed bridge.
An extension of the Luas to Cherrywood opened for passenger service on Saturday 16 October 2010, using most of the old railway alignment.
Bray Railway Station was renamed Bray Daly Railway Station in 1966 in honour of Edward Daly, one of the leaders of the 1916 Easter Rising.
The eastern platform features an interesting set of murals depicting the history of Ireland’s railways.
I am measuring any twist between the seattube and the headtube. .0035 inches is pretty much as good as it gets.
A view taken from Station Road, Mochdre in February 1984 where a brace of Metro-Cammell 2-car units was recorded working the BR Table 83 12:55 Llandudno Junction to Crewe. The train is running on the new track bed alignment installed to cater for the construction of the Landdulas to Glan Conwy [Colwyn By-Pass] section of the A55 North Wales Expressway.
All images on this site are exclusive property and may not be copied, downloaded, reproduced, transmitted, manipulated or used in any way without expressed written permission of the photographer. All rights reserved – Copyright Don Gatehouse
These images are from two very similar projects that I've just helped my friends with. They want to be able to hold bike frames on their alignment tables by the head tube. They both purchased used bench centers, and we had to make bull nose centers for them.
The fit up needed to be very close, so we were working to within a few tenths.
The other complication was that one of the centers on each set was spring loaded and utilized a rack and pinion to withdraw it... so we had to mill those rack gears.
U.S. Route 101, or U.S. Highway 101, is a north-south U.S. highway that runs through the states of California, Oregon, and Washington, on the West Coast of the United States. It is also known as El Camino Real (The Royal Road) where its route along the southern and central California coast approximates the old trail which linked the Spanish missions, pueblos and presidios. It merges at some points with California Highway 1.
In Southern California, the highway is a heavily traveled commuter route serving the Northwest portion of the Greater Los Angeles area; this includes Ventura County communities in the West Conejo Valley and in the Oxnard Plain, along with Los Angeles County communities in the East Conejo Valley and San Fernando Valley. The route is the Santa Ana Freeway from East Los Angeles to Downtown Los Angeles. It becomes the Hollywood Freeway north of Downtown Los Angeles through the Cahuenga Pass, before turning west and becoming the Ventura Freeway. Communities along the alignment include Hollywood and the southern edge of the San Fernando Valley, and the cities of Hidden Hills, Calabasas, Agoura Hills, Westlake Village, Thousand Oaks (includes Newbury Park), Camarillo, Oxnard, Ventura, and Santa Barbara. US 101 also integrates with the southern part of the historic El Camino Real.
en.wikipedia.org/wiki/U.S._101
en.wikipedia.org/wiki/Wikipedia:Text_of_Creative_Commons_...
Radcliffe Camera and St Mary's church point towards the bridge to Boathouse island in Christ Church Meadows
Sequence, structure, and phylogenetic analyses of various MtnDs.(A) Multiple sequence alignment of the MtnD family. Magenta bars show highly conserved cupin motifs I and II, magenta and green triangles show amino acid residues involved in metal binding and catalysis, respectively. (B) Predicted tertiary structure of T. thermophila MtnD. The structure of MtnD was modeled on that of M. musculus MtnD (PDB ID: 1VR3) using Swiss-model. Orange sphere represents a nickel atom. His307, His309, Glu313 and His352 (shown in magenta) form the metal binding site. Phe303, Arg315, and Phe 354 (shown in green) are essential for dioxygenation. (C) Phylogenetic tree based on primary sequences of the MtnD family. Alignments were created with ClustalW and displayed using Treeview. Scale bar indicates a difference of 0.1 substitutions per site. Names and gene accession numbers are as follows: A. gambiae (XP_315627), A. aeolicus (NP_214354), A. thaliana (NP_567443), A. nidulans (XP_664180), B. subtilis (NP_389245), C. elegans (NP_510072), C. variabilis (EFN58452), C. neoformans (XP_572521), D. rerio (NP_955962), D. discoideum (XP_635174), D. melanogaster (NP_001097577), E. cloacae 13047 (YP_003613573), G. kaustophilus (YP_146809), H. sapiens (NP_060739), H. magnipapillata (XP_002165167), K. oxytoca (AAD11793), M. aeruginosa (A8YMJ4), M. brevicollis MX1 (XP_001750499), M. musculus (NP_598813), N. crassa (XP_956660), O. sativa (AAC05511), P. aeruginosa (NP_250375), S. cerevisiae (NP_013722), S. pombe (NP_596475), S. purpuratus (XP_789320), S. elongatus (YP_171626), V. vinifera (CBI26309), V. carteri (XP_002955803), X. tropicalis (NP_001004933). Proteins described in (A) are underscored.
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BMW Tire Alignment Boca Raton. Shores Automotive proudly serves the Boca Raton community. We specialize in BMW repair and Mercedes Benz maintenance. We offer full diagnostics maintenance, motor work, brakes, cooling system, wheel alignment, tires, oil change, transmission repair, shock replacements and more.
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Domain structure, phylogeny and conservation of critical catalytic features of ciliate PIPKs.A, Domain structure of Tetrahymena PIPKs. The RING domain predicted in PIPK2b and transmembrane and SecY domains in PIPK5 have been removed (see Table S1 and Methods). Domain boundaries, e-values and further details are given in Table S1. B, Unrooted neighbor-joining tree of catalytic domains from 37 ciliate PIPKs. Bootstrap values from 5000 replicates higher than 60% are indicated near the corresponding branches. Group 1, 2, 3 and 4 PIPKs are color coded (blue, green, red and purple respectively). Circles and triangles represent Tetrahymena and Paramecium PIPKs, respectively. Bar indicates number of amino acid substitutions per site. Phylogenetic relationships of ciliate group 2 PIPK genes were less resolved with less nodes supported by high bootstrap values. In Paramecium, 4 additional group 3 PIPKs that are organized in 2 pairs of paralogs (PtPIPK3c,d and PtPIPK3e,f) and they are most related to TtPIPK3 are not shown. C, Sequence alignment of the catalytic kinase domains from ciliate PIPKs and mammalian PIPKI? and PIPKII?. The position of prominent catalytic features is indicated by arrows and arrowheads and numbered residues refer to the mouse PIPKII? structure described in reference 46. Polygons indicate residues that interact with ATP or the phosphoinositide substrate (PtdIns5P) in the crystal structure of PIPKII? and they are conserved in both type I and II PIPKs [46]. The variable inserts between the MDYSL and IID motifs present in all PIPKs have been omitted. The residues K150, D278 and D369, essential for catalytic activity, are conserved in all but 2 Tetrahymena PIPKs (highlighted in grey; see text and Figure S4 for details). The DLKGS motif in TtPIPK2c (highlighted in grey) has been reconstituted from RNA sequencing data (Table S1). The position of the KKxE/AxxxK motif in the specificity loop is indicated by a bar; further K residues that may contribute are highlighted by light blue and most ciliate PIPK1, but not PIPK2, genes confront to the consensus KK motif. Note that in all but 2 ciliate PIPKs the +2 position (E/A residues) in the specificity loop is occupied by a Glu residue as in all PtdInsP 5-kinases.
While out shooting Lake McDonald in Glacier National Park, I couldn't resist getting a shot of the setup on film. :)
Film: Kodak TMAX 100 (Expired 2010)
Camera: 1979 Olympus OM-2N
Processing: Caffenol-C-H
The Mr Despair on the right came from a manga cover. The D&D type alignments on the left came from some loving fan.(When I find the fan's name, I'll give them the credit.)
Putting them together made a rather nice wallpaper. For the week anyway.
DCIM\100GOPRO Old highway alignment in use before the construction of the current Currumbin Creek Bridge. Now called Thrower Drive.
Somewhere, deep in space The Alignment happens. Now nothing will be the same.
Mixed media original wall sculpture, created by me. Blue Lace Agate beads in the celestial body above the face. The face has eyes of Quartz Crystals. Bead work, polymer clay and paint on wood base.
Approx. 6.5 inches diameter.
The Carnac stones are an exceptionally dense collection of megalithic sites around the French village of Carnac, in Brittany, consisting of alignments, dolmens, tumuli and single menhirs. The more than 3,000 prehistoric standing stones were hewn from local rock and erected by the pre-Celtic people of Brittany, and are the largest such collection in the world.