View allAll Photos Tagged Substrate
This photo captures the scratched/streaked wort-agar substrate that the yeast cells grow on. The horizontal lines are obvious, but be aware that this is a crossroads. The point of streaking is to thin out the cell counts so you're depositing cell after cell. Here the vertical lines were streaked first, and then later the horizontal streaks pulled a few cells off course and made for nice little colonies. Fantastic!
I can't describe why I find this photo so important, but I do. It really is important. You can manipulate single-celled organisms with your hands. You can do this in your living room. This is yeast-ranching.
dark nodes are from poor (or excellent?) lighting. i.e. shadows.
FERTILE BODY growing at a substrate, formed by merging of smaller areas, can reach up to 30 cm in length. Are seen on the surface of tiny spikes up to 3 mm, light cream or tan color. Edge of the fruiting body is edged with a lighter shade.
MEAT: tinted from white to cream color, odor unspecified. Hard and tough.
HABITAT: vegetate the rotten and dead deciduous trees, usually in oak.
EDIBILITY: inedible mushrooms.
Substrate: Phellinus tremulae, Populus tremula.
Eesti punase nimestiku liik, ohualdis (VU). LK III.
Laudissalu, Põhja-Kõrvemaa.
Schizophyllum commune is probably the most widespread fungus in existence, being found on every continent except Antarctica, where there is no wood to be used as a substrate. There is a single common worldwide species, although there are a few less common species of Schizophyllum. The genus name means "split gill," and thus this is the split gill fungus. The species name "commune" actually does refer to shared ownership.
We know that there is a single widespread species because of the work of John Raper and his colleagues at Harvard University in the 1950's-1970's. They collected worldwide samples of this fungus. After collecting and germinating the spores into mycelium, they were able to get individuals from all over the world to mate with one another. During that time they were also able to divide the species in mating types (sexes) based on their mating reactions. As long as two strains are of different mating types they are able to mate and form fertile offspring.It does not appear to be very closely related to the other gilled mushrooms, and most researchers place it in its own order the Schizophyllales. The gills function to produce basidiospores on their surface. They appear to be split because they can dry out and rehydrate (and thus open and close) many times over the course of a growing season. The fruiting bodies to the right are probably a year old or more. This is a great adaptation for a climate with sporadic rains. Unlike other mushroom species, the mycelium only has to produce one set of fruiting bodies per year, which can then dry out and rehydrate and keep functioning. It's a great strategy for reproduction. You can probably even go out in the dead of winter and find sporulating fruiting bodies of this fungus. It's a very successful wood decay fungus that causes a white rot. Interestingly, this fungus has also been known to cause a human mycosis in just a few cases involving immunoincompetent people, especially children. In one case, the fungus had grown through the soft palate of a child's mouth and was actually forming fruiting bodies (mushrooms) in her sinuses!!!
We know that there is a single widespread species because of the work of John Raper and his colleagues at Harvard University in the 1950'2-1970's. They collected worldwide samples of this fungus. After collecting and germinating the spores into mycelium, they were able to get individuals from all over the world to mate with one another. During that time they were also able to divide the species in mating types (sexes) based on their mating reactions. As long as two strains are of different mating types they are able to mate and form fertile offspring.
See that in the bottom of the bag?
it's from a 5 gallon bucket of coffee grounds from Toscanini's in Cambridge.
This stuff seems to be working well as a mix-in substrate. I'm a bit concerned about the mold that grows on it. One observation I've made that may be useful is that the espresso seems more homogenous. Also, perhaps because they are stem pasturized as opposed to drip percolated, they seem to have less mold on them. they may also be pressed drier. In addition, I am uncertain about the coffee filters and how they will interact with the fungi. Furthermore, I'm not sure about the filters themselves and what they're made of. If the material is bleached, for example, that's something I think I would prefer to leave out of my cultivation process.
I consequently took two steps:
1) Called the company who makes the filters we use at home. I have yet to get a satisfactorily thorough response - only that they are 'safe for composing', and I felt that was more of a 'go away' answer than a well-informed one.
2) I have asked Toscanini's to set aside only their espresso waste.
Mugs and substrate from thrift shop
Flame quite difficult to photograph
Made for the mosaic challenge: www.flickr.com/groups/mosaicchallenge/discuss/72157609007...
Substrate: Phellinus tremulae, Populus tremula.
Eesti punase nimestiku liik, ohualdis (VU). LK III.
Mägede, Järvamaa.
Entry in category 1. Object of study; © CC-BY-NC-ND: Adrien Waelchli
On this image we can see a topography measurement of a crystalline substrate (NdGaO3) after an annealing of 15h00 at 1000°C in an oxygen flow. It was acquired using an atomic force microscope in a region of 10μm by 10μm. We can clearly distinguish a series of steps and terraces typical for this type of substrate.
What is quite incredible is that the height of each step is that of a single atomic layer, i.e. only 3.86Ă…. This substrate can then be used for the growth of thin films or superlattices.
A particularly interesting feature from an aesthetic point of view is the dynamics that emerge from this image.
Substrate of foam/concrete , base is MDF, and gloves are magic sculpt. I haven't grouted this yet-waiting for the mosaic to set.
Substrate is lifted on to the roof with a crane and spread on top of the Filter Mat.
The Green Roof Installation was carried out by Mark Bennetton and his team at Landmark Living Roofs www.landmarklivingroofs.co.uk/
The newest addition to the Cox Crew Zoo. Two little hermit crabs. We're letting them acclimate, so pictures aren't so great yet.
Wow - except for grout - it's done!!
It's roughly 4' x 16'!
It's an old bulletin board. We did the mosaics on a plywood substrate cut into 10 vertical strips, and then bolted them with tapcons through the thin plywood-y BB material already in place, and into the cinderblock, to make sure it's secure enough for that weight. The frame is pretty secure as well. I hung my entire body weight from it a couple of times and it supported my weight easily, with no sign of problem.
The kids did most of the mosaic (15" squares). I did the one on the lower row, 2nd from the right (sunset) and the art teacher did the one directly above it (bleeding heart flower). The main images were done entirely by students, and the backgrounds were done by a mixture of students and mosaic artists that I brought in to finish it more quickly (we started it in May 2008, and finished it in early January 2009). I did most of the background, with lots of help from students (5th - 8th grades). We used Weldbond for the mosaics, and silicon for the background tile.
The girl is one of the original artists who took part in the short course (she's standing just to the left of her mosaic). She also came in over the summer and worked on the backgrounds of the other mosaics.
A mass of Lahontan redside slowly move upstream along the bottom of Taylor Creek. What I thought was a spawning migration was actually a spawning aggregation. Fish close to the bottom will rapidly dart into the substrate where males press females against the substrate to simultaneously expel eggs and milt, then quickly rejoin the aggregation. This happens very quickly and is not readily observable with the use of a slow motion function. The colorful fish are very event from this angle, but it this mass of fish is nearly indiscernible from the bank or from above. This is because all the color is on the sides or undersides of the fish, while the back remains mostly grayish. The fertilized eggs are sticky and quickly sink to bottom where they adhere to rocks.
hardscaping complete after gravel is added above florendepot substrate. The rock is a green quartz that ive forgotten the name of
We found this trunk for $3.87 at a junk store. The wood stripping on the edges is in bad shape and falling off in places, and the trunk needs overall tightening. It's not pretty as is, but it will be perfect for mosaic!
The slats that make up the top of the trunk have slight gaps in them. I think I'm going to fill them in and mosaic it as a smooth surface. I've been considering using rigid wrap to make that happen, but I'd love to hear any other thoughts you have on how to achieve that.
Mammillaria heyderi var. heyderi found with bright red seed pods and closed blooms on a granite substrate in Gillespie County, Texas during mid March.
Rural school tissue culture facilities: Practising the transfer of plantlets to substrate, with the participation of teachers and students of different ages. From Case Study 2.6, on “Use of Tissue Culture in Cassava for Rural Households in Colombia” by R.H. Escobar, J. Restrepo, J. Tohme and W.M. Roca, in “Biotechnologies at Work for Smallholders: Case Studies from Developing Countries in Crops, Livestock and Fish”. 2013. Edited by J. Ruane, J.D. Dargie, C. Mba, P. Boettcher, H.P.S. Makkar, D.M. Bartley and A. Sonnino. 198 pp. FAO. www.fao.org/docrep/018/i3403e/i3403e00.htm. Photo credit: ©CIAT/R.H. Escobar. Copyright © CIAT/R.H. Escobar. Editorial use only. Photo credit must be given.