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15 February 2016, Rome, Italy - Gunter Pauli, Founder, Zero Emissions Research and Initiatives Network, Japan. FAO International Symposium on “The Role of Agricultural Biotechnologies in Sustainable Food Systems and Nutrition”, Opening Session. FAO headquarters (Green room).
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Global Forum on Biotechnology - The Evolving Promise of the Life Sciences
12 November 2012
OECD Conference Centre, Paris
The BIO World Congress on Industrial Biotechnology is the world’s largest industrial biotech gathering which brings together business executives, government officials, researchers and industry leaders from over 35 countries. This year's event was held in Orlando, Florida from April 29 – May 2, 2012 at the Gaylord Palms
Nina Federoff speaking at the International Association of Plant Biotechnology (IAPB) 2014 Congress in Melbourne, Victoria, Australia.
JAMSHID RAHEB
9041 YONGE ST,
RICHMOND HILL,
ON, CANADA,
L4C 0L2
jamshid_raheb@yahoo.com
jam@nigeb.ac.ir
(905)886-2338
(416) 587-3032
:
Enclosed please find my resume;
I graduated from the University of Warwick from United Kingdom in May 1998 with a PhD Degree in molecular microbiology. My major was Genetic Engineering. As you will see from my resume, in addition to meeting the demands of a full-time academic schedule, I have also accumulated fifteen years of experience in biotechnology research.
Sincerely,
Jamshid Raheb
Jamshid Raheb
PersonalDate and Place of Birth: March 17-1962 – Tehran – IRAN
Marital Status: Single
ObjectiveSearching, learning and teaching
Education
Research work
Ph. D in Molecular Microbiology
1994-1998 The University of Warwick ,U.K. ENGLAND
-Thesis: The molecular and physiological effects of starvation and other stresses on
Flexibacter chinensis
Average grade: A+
For many years physiological, morphological and particularly molecular studies on
gliding bacteria were impossible due to the lack of a suitable technique for the genetic
manipulation of this group of bacteria including Flexibacter chinensis. Our study has focused
on the physiological and morphological changes in molecular level in this organism during
the transition from the exponential to stationary phase of growth, and during long -term stress
condition. Our data demonstrate that there is a dramatic change in respiration activity and
enzymes during the stationary phase which can not be seen in the exponential phase.
Parallel to this bacterial morphology changes with a particularly noticeable size
reduction in this usually filamentous bacterium. With the collaboration of Wisconsin
University in USA we have developed a method for the molecular study of this group of bacteria.
We have constructed two mutants for the genes which produce two novel sigma factors, KatF(sS)
and RpoH(s32), and have investigated the effect of these mutants with respect to cell size
changes during the transition from growth to starvation conditions and under heat stress.
The mutants were unable to change from the filamentous to coccoidal form and consequently
were unable to survive stress as effectively as the parental strain which could undergo
morphological changes. Our data have demonstrated the importance of cell division in
Flexibacter chinensis for the survival of this bacterium under stress conditions.
Master of Science (M.Sc) in Bacteriology
1987-1991, Tarbiat Modarres University, Tehran, IRAN
Average grade: 17.62 / 20
-Thesis: Investigation of fastidious E. coli in the urinary tract infections
Thesis Grade: 19.50 / 20
Bachelor of Science in Biology (B.Sc) in Microbiology
Aug. 1983 – Jul. 1987, Tehran University , Tehran, IRAN
Average grade: 17.60 / 20
The Microbial sulfur-specific transformation which selectively desulfurize organic sulfur compounds in fossil fuels, has been identified. We have Identified, Isolated, cloned and sequenced a 4kb gene locus from a newly isolated Iranian strain Rhodococcus FMF(4S pathway genes) on a broad host range plasmid with integration ability in chromosome of gram negative bacteria. This was reported for the first time as chromosomal conserved dsz genes from 4S pathway in Rhodococcus which these genes recorded in EMBL(1,2,3,4). This organized as one operon with three genes (dszA, dszB and dszC)under the control of single promoter. The dsz gene from this new strain has been cloned under control of tac promoter in different pseudomonas solvent tolerant species(ATCC 9027). We have applied an industrial microorganism , with the ability in biological desulfurization. In this way, using molecular and microbiological techniques we successfully constructed a manipulated bacterium with two relevant industrial application, that is biosurfactant production and desulfurization of fossil fuel. I have selected a strain of pseudomonas. Sp. which is producing biosurfactants which can help the desulfurization process by accelerating phase separation. In this regard I have cloned Rhodococcus Erytheropolis(IGTS8) desulfurization operon with a heterologues promoter(tac) in this pseudomonas strain. The resulted construct has three industrial traits. First, it has an enhanced desulfurization activity due to the exchange of native promoter with heterologues tac promoter. Second, it can produce biosurfactants which in turn help to desulfurization process by enhancing phase separation. Third, as pseudomonas Sp. is the most known solvent tolerance bacteria, this construct has the advantage to be used in industrial operation. In further step I am going to conduct a research on likely interactions between Rhamnolipid biosurfactants and oil components. Further researches is in progress to find out the influence of the biosurfactants on DBT desulfurization. By the way I am to appreciate the close cooperation of Dr Diaz from Spain. This is the new alternatives to commercialize the biodesulfurization of crude oil processes.
LanguagesEnglish, Turkish and Farsi.
Skills
Molecular cloning, Polymerase chain reaction (PCR), DNA sequencing, Southern hybridization, Northern hybridization, Western blots, DNA separation techniques, Two-dimensional gel electrophoresis (2D Gels) DNA library, Over-expression of proteins, MALDI-TOF.
Work Experience
National Institute for Genetic Engineering and Biotechnology (NIGEB) of IRAN. March 1998 – 2007 (Dept. of Microbiology), Member of Faculty Board ,
Principal Investigator (PI) several research projects
Teaching the course units related to Molecular Microbiology and Biotechnology
Workshop attended“Good Laboratory Practice (GLP)”, NIGEB, Sep.(17-19) 2000, Tehran-IRAN
“Cloning in bacterial expression system for production of recombinant protein” Organized by
NIGEB, Jan (18-22) 2003. Tehran- IRAN
“Application of Enzymes in Biotechnology” Organized by NIGEB, Dec (23-27)
2006. Tehran – IRAN
Papers1- Raheb, J. and Yakhchali, B. (2002). Genetic analysis of biodesulfurizing bacteria isolated
in IRAN. Journal of Sciences IslamicRepublic of Iran, 11, No 3 and 4, 1-12.
2- Akbarzadeh, A., Raheb, J., Aghaei, E. and Karkhane, A. (2003). Study ofDesulfurization rate
in Rhodococcus FMF Native Bacterium, Iranian Journal of Biotechnology , 1, 1, 36-40.
3- Kazemi, M and Raheb, J. (2004), Isolation and Identification of a Sulfide/Sulfoxide
Monooxigenase Gene from a Newly Isolated Rhodococcus Sp, Strain FMF. Journal of Sciences,
Islamic Republic of Iran, 15, 2, 119-126.
4- Naghdi, S., Raheb, J., Yakhchali, B. and Aghaei, E. (2004). Cloning of Desulfurization Operon
of 4S pathway in Pseudomonas.(In Persian Language), Journal of Science ( Tehran University),
30, 389-391.
5- Akbarzadeh, A., Raheb, J., Rastegar, Jazi, (2004). Cloning and sequencing of desulfurization
Operon from a Newly Isolated Bacterium Rhodococcus FMF, Iranian Journal of Biotechnology ,
Vol 2, No.1, 19-24
6- Raheb, J., Yektamaram, L., Shaabani, A., Akbari, M and Aghaei, E.(2004), Isolation and
cloning of Glocose oxidase gene from Aspergillus niger In Ecoli. Koomesh Journal of semnan
University of Medical Sciences, 5, 1&2, 29-36.
7- Akbarzadeh, A., Raheb (2004). Identification and Cloning of desulfurization Operon (dszAB)
from a Native Bacterium Rhodococcus FMF, . Koomesh Journal of semnan University of
Medical Sciences, Spring & Summer 2004,Vol.5, No. 3&4, 171-178.
8- Raheb, J., Mohaghegh. A., Akbari, M., Shabani,and Aghaee, E (2005). Cloning of
Glucose oxidase gene in pkk233-3. . Koomesh Journal of semnan University of
Medical Sciences, Vol 6, No 3, 217-222.
9- Raheb, J., Naghdi, S., Yakhchali, B., (2005). Designing a new Recombinant strain with
additional copy number to enhance biodesulfurization activity in Pseudomonas
aeruginosa ATCC9027. Iranian Journal of Science & Technology, 29, 195-199.
10- Raheb, J., Karkhane, A.A., Naghdi, S., Flint, K., (2005). The mutation of the rpoS gene,
the central regulator of stationary phase, affects the cell division in Flexibacter chinensis,
Journal of Sciences,16, 209-215.
11- Raheb, J. Naghdi, S. and Flint, K.P. (2006). "De novo protein production under long
term starvation in Flexibacter chinensis". Mendez-Vilas, Proceedings Book titled "Modern
Multidisciplinary Applied Microbiology, 50-54.
12- Raheb, J., Aghaei, E. S., Ghorbanli, M., Khanyeki, G., and Naghdi,(2006).
Identification and cloning of oxidoreductase gene from Rhodococcus erythropolis in
E.coli. Koomesh Journal of semnan University of Medical Sciences, 6, 4, 325-334.
13- Raheb, J. Naghdi, S. and Flint, K.P. (2006). Alkaline phosphatase activity of Flexibacter
chinensis under starvation stress in strile water microcosoms. special issue of the Enzyme and
Microbial Technology journal, 40, 13-16.
14- Raheb, J., Naghdi, S. and Flint, K.(2006). The effect of starvation factor on the survival
characteristics of Flexibacter chinensis, Iranian Journal of Science & Technology.
Transaction A. Vol. 31, No. A1, 117-121
15- Raheb, J., Naghdi, S. and Flint, K.(2006). The effect of some environmental factors
on the survival characteristics of Flexibacter chinensis. Journal of Sciences.
Islamic Republic of Iran, 17, 2, 317-322.
16- Raheb, J., Naghdi, S. and Flint, K.(2007). Respiration Activity in Flexibacter chinensis under
Starvation Stress, Iranian Journal of Science & Technology. Transaction A, Vol. 31, No.A3, 1-10
17- Raheb, J., Naghdi, S. Karkhane, A.A., Yahkchali , B., and Flint, K.P. (2004).
nucleotide sequence of dszC Gene from a Newly Isolated Rhodococcus FMF, Shiraz
E -Medical Journal, 5, 2.
18- Raheb, J., Naghdi, S. and Flint, K. (2006). Heat-Induced Synthesis of δ32 in Flexibacter
chinensis: The Role and Function in cell division, Journal of Chemistry and Chemical
Engineering, 18, 2, 115-121.
19- Raheb, J., Naghdi, S. and Flint, K.(2008). The effect of starvation stress on the protein profiles in
Flexibacter chinensis. Iranian biomedical Journal .12, 2, 67-77
20- Akbari Noghabi, K., Shahbani Zahiri, H., Raheb, J., Nasri, S. and Chul Yun, S.(2007).
The Survey of Mercury Obsorption by pseudomonas flourescens BM07 grown at two different
Temperature, Microbiological Research, 56, NO 2, 111-117.
21- Raheb, J., khoshroo., H , Nasernejad., B, Sanati., M,H, Azimi., A, Naghdi., S. and
Arabnezhad., M..(2006). Isolation and Characterization of a new Acidothiobacillus
Thiooxidans from the Aliabad copper mine in Yazd using 16s-23s spacer gene nucleotide
sequencing method, Journal of Chemistry and Chemical Engineering.
22- Raheb, J., khoshroo, H., Nasernejad, B., Sanati, MH., Azimi, A., Naghdi,
S. and Arabnezhad, M..(2007). Isolation and Characterization of a new Acidothiobacillus
Ferrooxidans from the Aliabad copper mine in Yazd using 16s-23s spacer gene nucleotide
sequencing method, Journal of Sciences, Islamic Republic of Iran, 18(3): 209-213
23- Barzegar, F., Raheb. J. and Mosavi, M.L. (2007). Cloning and expression of SOX(dsz A B, C)
Operon in Ecoli Strain DH5a and comparison its desulfurization activity with pseudomonas aeroginosa
EGSOX, Pseudomonas putida EGSOX and Ecoli cc118 lambda pir,. Koomesh Journal of semnan University
of Medical Sciences, Spring & Summer 2007,Vol.8, No. 2, 1-15.
24- Raheb, J., Aghaei, E. S. and Behzadi, H,(2008), Enhansment of Biodesulfurization activity via the overexpression of the oxidoreductase gene from R.Eytheropolis IGTS8 using pet21a vector, Koomesh Journal of semnan University
of Medical Sciences, Winter 2008,Vol.9, No. 2, 1-15, 99-105.
25- Shourian, M., Akbari Noghabi, K., Shahbani Zahiri, H., Bagheri, T., Karballaei, G.,
Mollaei, M., Rad, I., Ahadi, S., Raheb. J. and Abbasi, H. (2008). DES 5233 Efficient
Phenol-degradation by a newly characterized Pseudomonas sp. SA01 isolated from pharmaceutical
Wastewaters. Desalination Journal
Presentations1- Raheb, J., Aghaei, E., Naghdi, S. and Yakhchali, B. (2001). Cloning and Expression of
desulfurization Operon in Pseudomonas aeroginusa to Enhance Biodesulfurization Rate in Fossil
Fuels, 2th National Biotechnology Congress IslamicRepublicof Iran, Oct. 9-11 2001, Karaj
Agricultural Biotechnology Research Institute of Iran
2- Hosseini, Sayyedabolfazl., Yakhchali, B., Sattari. Morteza and Raheb, J. (2001). Cloning of
DNA Fragment Encoding NSP4 Epitope of Rotavirusin CS3Fimberial Subunit Gene of
Enterotoxigenic E.coli, 2th NationalBiotechnology Congress IslamicRepublicof Iran, Oct.
9-11 2001, Karaj Agricultural Biotechnology Research Institute of Iran
3- Raheb, J. and Yakhchali, B. (2002). Genetic analysis of biodesulfurizing bacteria isolated in
IRAN. Ninth Iranian Biology Conference, Faculty of Science, University of Tehran, 15-17 August 2000.
4- Raheb, J., Akbarzadeh, S. and Flint, K.P. (2003). Consevation of chromosomal-Encoded
Dibenzothiophene desulfurization genes in a newly Isolated Bacterium Rhodococcus FMF.
10th Iranian Researchers seminar in Europe (IRSE2003), Gonville and Cauis college University
of Cambridge Cambridge 31 May, 2003
5- Akbarzadeh, A., Raheb, J., Aghaei, E. and Karkhane, A. (2003). Study and Compare of
Desulfurization rate in Rhodococcus FMF Native Bacterium, 7th National Iran chemical
Engineering Congress, University of Tehran 28-31 October, 2002
6- Raheb, J., Aghaei, E. and Naghdi, S. (2004). Identification and Cloning of
Oxidoreductase gene from Rhodococcus Erytheropolis, Proceedings of The 12th Iranian biology
Conference.
7- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Alkaline phosphatase activity of Flexibacter
chinensis under starvation stress in strile water microcosoms. 1tst International Conference on
Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005). March
5-18th 2005, Badajoz, Spain
8- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The effect of stress on the protein profiles in
Flexibacter chinensis. 1tst International Conference on Environmental , Industrial and
Applied Microbiology(BioMicroWorld-2005). March 15-18th 2005, Badajoz, Spain.
9- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Heat-Induced Synthesis of δ32 in
F. chinensis: The Role and Function in cell Division . 1tst International Conference
on Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005).
March 15-18th 2005, Badajoz, Spain.
10- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Respiration Activity in Flexibacter chinensis
under Starvation Stress 1tst International Conference on Environmental , Industrial and
Applied Microbiology(BioMicroWorld-2005). March 15-18th 2005, Badajoz, Spain.
11- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The mutation of the ropS gene, the central
regulator of stationary phase, affects the cell division in F. chinensis 1tst International
Conference Environmental Industrial and Applied Microbiology(BioMicroWorld-2005). March
15-18th 2005, Badajoz, Spain.
12- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The effect of environmental factors on
the survival characteristics of Flexibacter chinensis. 1tst International Conference
on Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005).
March 15-18th 2005, Badajoz, Spain.
13- Raheb, J., Aghaei, E., Khaniki, GH.. Ghorbanly, M. and Naghdi, S. (2005). Identification
and Cloning of Oxidoreductase gene from Rhodococcus erythropolis in E.coli .
The 4th National Biotechnology Congress Islamic Republic of Iran, 15-17 Auguest 2005,
Kerman (Mahan).
14- Nazari. B., Haghbeen. K., Raheb, J., Arab Najafi. S. and Fatemi, S.S. (2005).
Optimization Cholesterol Oxidase production by Rhodococcus erythropolis ATCC 4277
by using Taguchi Statistical Nethod. The 4th National Biotechnology Congress Islamic
Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
15- Behzadi. H., Raheb, J., Aghaei, E. and Akbarzadeh, S. (2005). Cloning and Expression of
Oxidoreductase gene from Rhodococcus erythropolis IGTS8 for Enhancement of Biological
Desulfurization. The 4th National Biotechnology Congress Islamic Republic of Iran,
15-17 Auguest 2005, Kerman (Mahan).
16- Mohaghegh. A., Raheb, J., Khaniki, GH.. Akbari, M., Shabani, A and Aghaei, E., (2005).
Cloning of Glucose oxidase Aspergillus Niger in Prokariotic Host. The 4th National
Biotechnology Congress Islamic Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
17- Ghaffari. S., Raheb, J., Akbarzadeh, S. and Ebrahimi, GH. (2005). Desulfurization Process
of Pesudomonas aeroginosa in presence of Biosurfactant . The 4th National Biotechnology
Congress Islamic Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
18- Behzadi. H., Raheb, J., Aghayemoghadam, E. and Akbarzadeh, S. (2005). Increasing The
copy Number of Oxidoreductase gene for Importante the Expression of Desulfurization
Genes. 13th Iranian biology Conference and the first International Conference of Biology
Guilan University, Rasht, I.R.Iran, 23-25 august 2005.
19- Raheb, J., Mohaghegh. A., Akbari, M., Shabani, A., Khodabandeh, M and Karkhanei,
A. (2005). Cloning and Expression of Glucose Oxidase Aspergillus niger in E.coli.
13th Iranian biology Conference and the first International Conference of Biology Guilan
University, Rasht, I.R.Iran, 23-25 august 2005.
20- Rasehk, B., Mosavi, MT. and Raheb, J.(2006). Investigation of Biodesulfurization operon
activity in the Rhodococcus Erytheropolis IGTS8 influence of Oxidoreductase gene.
14th National and 2th InternationalConfrence of Biology Tarbiat Modares University,
Tehran, Iran, 2006.
21- Yektamaram, L., Raheb, J., Akbari, Shaabani, A., M and Aghaei, E.(2004), Isolation
and cloning of Glocose oxidase gene from Aspergillus niger In Ecoli. The 3th National
Biotechnology Congress Islamic Republic of Iran, 11-13 September 2004, Mashhad
21- Akbarzadeh, A., Raheb, J.,(2004),Determination of genetic map and Nucleotide sequences
of dszAB in Rhodococcus FMF a native bacteria in IRAN. The 3th National Biotechnology
Congress Islamic Republic of Iran, 11-13 September 2004, Mashhad
23- Jamshid Raheb; Hasan khoshroo; Ali Azimi; Bahram Nasernejad; Mohammad
hossein Sanati; Mahmood Arabnezhad (2007). Isolation and Characterization of a new
Acidothiobacillus Ferrooxidans from the Aliabad copper mine in Yazd using 16s-23s
spacer gene nucleotide sequencing method. II International Conference Environmental
Industrial and Applied Microbiology(BioMicroWorld-2007). 28 November -1December 2007,
Seville , Spain.
24- Jamshid Raheb; Hasan khoshroo; Ali Azimi; Bahram Nasernejad; Mohammad
hossein Sanati; Mahmood Arabnezhad (2007). Isolation and characterization of novel
fastidious, acidophilic species from the Aliabad copper mine in Yazd using 16s-23s
intergenic, spacer gene nucleotide sequencing method . II International Conference
Environmental Industrial and Applied Microbiology(BioMicroWorld-2007). 28 November –
1December 2007, Seville , Spain.
Gene Submission 1- AJ514873.3
2- AJ514949.2
3- AJ514947.2
4- AJ514948.1
5- AJ973326.1
6- AJ973325.1
7- AJ973323.1
8- AJ973324.1
9- AM039687.1
10- AM039688.1
11- AM296495
12- AM296496
13- AM296497
14- AM296498
15- AM296499
16- AM404265
17- AM404266
18- AM404267
19 - AM404268
20- AM404269
21- AM404270
22- AM404271
23- AM404272
24- AM404273
25- AM404274
26- FM946026
27- FM946027
Patents
1- 38302003
2- 38302004
3- 38302005
4- 38303079
1- Khosrow Adeli
Dept of Pediatric
Laboratory Medicine professor
University Of Toronto
555 University Ave
Toronto, Ontario
Canada M5G 1X8
Email: khosro.adeli@sickkids.ca
Tel: (416) 813 8682
Pager: (416) 232 4150
Fax: (416) 813 6257
2- Hojatollah Vali Ph.D.
Director
Faculty for Electron Microscopy Research
McGill University
3640 University Street
Montreal, OC H3A 2B2
Email: hojatollah.vali@mcgill.ca
www.medicine.mcgill.ca /femr
Tel: (514) 398 3025
Fax: (514) 398 5047
3- Nasser Khodai Booran Ph. D
The Hospital for Sick Children
Division Of Infection Diseases
Researcher
555 University Ave
Toronto, Ontario
Canada M5G 1X8
Email: nkhodaibooran@yahoo.com
Nasser.khodaibooran@sickkids.ca
Tel: (416) 813 6009
Pager: (416) 232 4150
Fax: (416) 813 5993
JAMSHID RAHEB
9041 YONGE ST,
RICHMOND HILL,
ON, CANADA,
L4C 0L2
jamshid_raheb@yahoo.com
jam@nigeb.ac.ir
(905)886-2338
(416) 587-3032
:
Enclosed please find my resume;
I graduated from the University of Warwick from United Kingdom in May 1998 with a PhD Degree in molecular microbiology. My major was Genetic Engineering. As you will see from my resume, in addition to meeting the demands of a full-time academic schedule, I have also accumulated fifteen years of experience in biotechnology research.
Sincerely,
Jamshid Raheb
Jamshid Raheb
PersonalDate and Place of Birth: March 17-1962 – Tehran – IRAN
Marital Status: Single
ObjectiveSearching, learning and teaching
Education
Research work
Ph. D in Molecular Microbiology
1994-1998 The University of Warwick ,U.K. ENGLAND
-Thesis: The molecular and physiological effects of starvation and other stresses on
Flexibacter chinensis
Average grade: A+
For many years physiological, morphological and particularly molecular studies on
gliding bacteria were impossible due to the lack of a suitable technique for the genetic
manipulation of this group of bacteria including Flexibacter chinensis. Our study has focused
on the physiological and morphological changes in molecular level in this organism during
the transition from the exponential to stationary phase of growth, and during long -term stress
condition. Our data demonstrate that there is a dramatic change in respiration activity and
enzymes during the stationary phase which can not be seen in the exponential phase.
Parallel to this bacterial morphology changes with a particularly noticeable size
reduction in this usually filamentous bacterium. With the collaboration of Wisconsin
University in USA we have developed a method for the molecular study of this group of bacteria.
We have constructed two mutants for the genes which produce two novel sigma factors, KatF(sS)
and RpoH(s32), and have investigated the effect of these mutants with respect to cell size
changes during the transition from growth to starvation conditions and under heat stress.
The mutants were unable to change from the filamentous to coccoidal form and consequently
were unable to survive stress as effectively as the parental strain which could undergo
morphological changes. Our data have demonstrated the importance of cell division in
Flexibacter chinensis for the survival of this bacterium under stress conditions.
Master of Science (M.Sc) in Bacteriology
1987-1991, Tarbiat Modarres University, Tehran, IRAN
Average grade: 17.62 / 20
-Thesis: Investigation of fastidious E. coli in the urinary tract infections
Thesis Grade: 19.50 / 20
Bachelor of Science in Biology (B.Sc) in Microbiology
Aug. 1983 – Jul. 1987, Tehran University , Tehran, IRAN
Average grade: 17.60 / 20
The Microbial sulfur-specific transformation which selectively desulfurize organic sulfur compounds in fossil fuels, has been identified. We have Identified, Isolated, cloned and sequenced a 4kb gene locus from a newly isolated Iranian strain Rhodococcus FMF(4S pathway genes) on a broad host range plasmid with integration ability in chromosome of gram negative bacteria. This was reported for the first time as chromosomal conserved dsz genes from 4S pathway in Rhodococcus which these genes recorded in EMBL(1,2,3,4). This organized as one operon with three genes (dszA, dszB and dszC)under the control of single promoter. The dsz gene from this new strain has been cloned under control of tac promoter in different pseudomonas solvent tolerant species(ATCC 9027). We have applied an industrial microorganism , with the ability in biological desulfurization. In this way, using molecular and microbiological techniques we successfully constructed a manipulated bacterium with two relevant industrial application, that is biosurfactant production and desulfurization of fossil fuel. I have selected a strain of pseudomonas. Sp. which is producing biosurfactants which can help the desulfurization process by accelerating phase separation. In this regard I have cloned Rhodococcus Erytheropolis(IGTS8) desulfurization operon with a heterologues promoter(tac) in this pseudomonas strain. The resulted construct has three industrial traits. First, it has an enhanced desulfurization activity due to the exchange of native promoter with heterologues tac promoter. Second, it can produce biosurfactants which in turn help to desulfurization process by enhancing phase separation. Third, as pseudomonas Sp. is the most known solvent tolerance bacteria, this construct has the advantage to be used in industrial operation. In further step I am going to conduct a research on likely interactions between Rhamnolipid biosurfactants and oil components. Further researches is in progress to find out the influence of the biosurfactants on DBT desulfurization. By the way I am to appreciate the close cooperation of Dr Diaz from Spain. This is the new alternatives to commercialize the biodesulfurization of crude oil processes.
LanguagesEnglish, Turkish and Farsi.
Skills
Molecular cloning, Polymerase chain reaction (PCR), DNA sequencing, Southern hybridization, Northern hybridization, Western blots, DNA separation techniques, Two-dimensional gel electrophoresis (2D Gels) DNA library, Over-expression of proteins, MALDI-TOF.
Work Experience
National Institute for Genetic Engineering and Biotechnology (NIGEB) of IRAN. March 1998 – 2007 (Dept. of Microbiology), Member of Faculty Board ,
Principal Investigator (PI) several research projects
Teaching the course units related to Molecular Microbiology and Biotechnology
Workshop attended“Good Laboratory Practice (GLP)”, NIGEB, Sep.(17-19) 2000, Tehran-IRAN
“Cloning in bacterial expression system for production of recombinant protein” Organized by
NIGEB, Jan (18-22) 2003. Tehran- IRAN
“Application of Enzymes in Biotechnology” Organized by NIGEB, Dec (23-27)
2006. Tehran – IRAN
Papers1- Raheb, J. and Yakhchali, B. (2002). Genetic analysis of biodesulfurizing bacteria isolated
in IRAN. Journal of Sciences IslamicRepublic of Iran, 11, No 3 and 4, 1-12.
2- Akbarzadeh, A., Raheb, J., Aghaei, E. and Karkhane, A. (2003). Study ofDesulfurization rate
in Rhodococcus FMF Native Bacterium, Iranian Journal of Biotechnology , 1, 1, 36-40.
3- Kazemi, M and Raheb, J. (2004), Isolation and Identification of a Sulfide/Sulfoxide
Monooxigenase Gene from a Newly Isolated Rhodococcus Sp, Strain FMF. Journal of Sciences,
Islamic Republic of Iran, 15, 2, 119-126.
4- Naghdi, S., Raheb, J., Yakhchali, B. and Aghaei, E. (2004). Cloning of Desulfurization Operon
of 4S pathway in Pseudomonas.(In Persian Language), Journal of Science ( Tehran University),
30, 389-391.
5- Akbarzadeh, A., Raheb, J., Rastegar, Jazi, (2004). Cloning and sequencing of desulfurization
Operon from a Newly Isolated Bacterium Rhodococcus FMF, Iranian Journal of Biotechnology ,
Vol 2, No.1, 19-24
6- Raheb, J., Yektamaram, L., Shaabani, A., Akbari, M and Aghaei, E.(2004), Isolation and
cloning of Glocose oxidase gene from Aspergillus niger In Ecoli. Koomesh Journal of semnan
University of Medical Sciences, 5, 1&2, 29-36.
7- Akbarzadeh, A., Raheb (2004). Identification and Cloning of desulfurization Operon (dszAB)
from a Native Bacterium Rhodococcus FMF, . Koomesh Journal of semnan University of
Medical Sciences, Spring & Summer 2004,Vol.5, No. 3&4, 171-178.
8- Raheb, J., Mohaghegh. A., Akbari, M., Shabani,and Aghaee, E (2005). Cloning of
Glucose oxidase gene in pkk233-3. . Koomesh Journal of semnan University of
Medical Sciences, Vol 6, No 3, 217-222.
9- Raheb, J., Naghdi, S., Yakhchali, B., (2005). Designing a new Recombinant strain with
additional copy number to enhance biodesulfurization activity in Pseudomonas
aeruginosa ATCC9027. Iranian Journal of Science & Technology, 29, 195-199.
10- Raheb, J., Karkhane, A.A., Naghdi, S., Flint, K., (2005). The mutation of the rpoS gene,
the central regulator of stationary phase, affects the cell division in Flexibacter chinensis,
Journal of Sciences,16, 209-215.
11- Raheb, J. Naghdi, S. and Flint, K.P. (2006). "De novo protein production under long
term starvation in Flexibacter chinensis". Mendez-Vilas, Proceedings Book titled "Modern
Multidisciplinary Applied Microbiology, 50-54.
12- Raheb, J., Aghaei, E. S., Ghorbanli, M., Khanyeki, G., and Naghdi,(2006).
Identification and cloning of oxidoreductase gene from Rhodococcus erythropolis in
E.coli. Koomesh Journal of semnan University of Medical Sciences, 6, 4, 325-334.
13- Raheb, J. Naghdi, S. and Flint, K.P. (2006). Alkaline phosphatase activity of Flexibacter
chinensis under starvation stress in strile water microcosoms. special issue of the Enzyme and
Microbial Technology journal, 40, 13-16.
14- Raheb, J., Naghdi, S. and Flint, K.(2006). The effect of starvation factor on the survival
characteristics of Flexibacter chinensis, Iranian Journal of Science & Technology.
Transaction A. Vol. 31, No. A1, 117-121
15- Raheb, J., Naghdi, S. and Flint, K.(2006). The effect of some environmental factors
on the survival characteristics of Flexibacter chinensis. Journal of Sciences.
Islamic Republic of Iran, 17, 2, 317-322.
16- Raheb, J., Naghdi, S. and Flint, K.(2007). Respiration Activity in Flexibacter chinensis under
Starvation Stress, Iranian Journal of Science & Technology. Transaction A, Vol. 31, No.A3, 1-10
17- Raheb, J., Naghdi, S. Karkhane, A.A., Yahkchali , B., and Flint, K.P. (2004).
nucleotide sequence of dszC Gene from a Newly Isolated Rhodococcus FMF, Shiraz
E -Medical Journal, 5, 2.
18- Raheb, J., Naghdi, S. and Flint, K. (2006). Heat-Induced Synthesis of δ32 in Flexibacter
chinensis: The Role and Function in cell division, Journal of Chemistry and Chemical
Engineering, 18, 2, 115-121.
19- Raheb, J., Naghdi, S. and Flint, K.(2008). The effect of starvation stress on the protein profiles in
Flexibacter chinensis. Iranian biomedical Journal .12, 2, 67-77
20- Akbari Noghabi, K., Shahbani Zahiri, H., Raheb, J., Nasri, S. and Chul Yun, S.(2007).
The Survey of Mercury Obsorption by pseudomonas flourescens BM07 grown at two different
Temperature, Microbiological Research, 56, NO 2, 111-117.
21- Raheb, J., khoshroo., H , Nasernejad., B, Sanati., M,H, Azimi., A, Naghdi., S. and
Arabnezhad., M..(2006). Isolation and Characterization of a new Acidothiobacillus
Thiooxidans from the Aliabad copper mine in Yazd using 16s-23s spacer gene nucleotide
sequencing method, Journal of Chemistry and Chemical Engineering.
22- Raheb, J., khoshroo, H., Nasernejad, B., Sanati, MH., Azimi, A., Naghdi,
S. and Arabnezhad, M..(2007). Isolation and Characterization of a new Acidothiobacillus
Ferrooxidans from the Aliabad copper mine in Yazd using 16s-23s spacer gene nucleotide
sequencing method, Journal of Sciences, Islamic Republic of Iran, 18(3): 209-213
23- Barzegar, F., Raheb. J. and Mosavi, M.L. (2007). Cloning and expression of SOX(dsz A B, C)
Operon in Ecoli Strain DH5a and comparison its desulfurization activity with pseudomonas aeroginosa
EGSOX, Pseudomonas putida EGSOX and Ecoli cc118 lambda pir,. Koomesh Journal of semnan University
of Medical Sciences, Spring & Summer 2007,Vol.8, No. 2, 1-15.
24- Raheb, J., Aghaei, E. S. and Behzadi, H,(2008), Enhansment of Biodesulfurization activity via the overexpression of the oxidoreductase gene from R.Eytheropolis IGTS8 using pet21a vector, Koomesh Journal of semnan University
of Medical Sciences, Winter 2008,Vol.9, No. 2, 1-15, 99-105.
25- Shourian, M., Akbari Noghabi, K., Shahbani Zahiri, H., Bagheri, T., Karballaei, G.,
Mollaei, M., Rad, I., Ahadi, S., Raheb. J. and Abbasi, H. (2008). DES 5233 Efficient
Phenol-degradation by a newly characterized Pseudomonas sp. SA01 isolated from pharmaceutical
Wastewaters. Desalination Journal
Presentations1- Raheb, J., Aghaei, E., Naghdi, S. and Yakhchali, B. (2001). Cloning and Expression of
desulfurization Operon in Pseudomonas aeroginusa to Enhance Biodesulfurization Rate in Fossil
Fuels, 2th National Biotechnology Congress IslamicRepublicof Iran, Oct. 9-11 2001, Karaj
Agricultural Biotechnology Research Institute of Iran
2- Hosseini, Sayyedabolfazl., Yakhchali, B., Sattari. Morteza and Raheb, J. (2001). Cloning of
DNA Fragment Encoding NSP4 Epitope of Rotavirusin CS3Fimberial Subunit Gene of
Enterotoxigenic E.coli, 2th NationalBiotechnology Congress IslamicRepublicof Iran, Oct.
9-11 2001, Karaj Agricultural Biotechnology Research Institute of Iran
3- Raheb, J. and Yakhchali, B. (2002). Genetic analysis of biodesulfurizing bacteria isolated in
IRAN. Ninth Iranian Biology Conference, Faculty of Science, University of Tehran, 15-17 August 2000.
4- Raheb, J., Akbarzadeh, S. and Flint, K.P. (2003). Consevation of chromosomal-Encoded
Dibenzothiophene desulfurization genes in a newly Isolated Bacterium Rhodococcus FMF.
10th Iranian Researchers seminar in Europe (IRSE2003), Gonville and Cauis college University
of Cambridge Cambridge 31 May, 2003
5- Akbarzadeh, A., Raheb, J., Aghaei, E. and Karkhane, A. (2003). Study and Compare of
Desulfurization rate in Rhodococcus FMF Native Bacterium, 7th National Iran chemical
Engineering Congress, University of Tehran 28-31 October, 2002
6- Raheb, J., Aghaei, E. and Naghdi, S. (2004). Identification and Cloning of
Oxidoreductase gene from Rhodococcus Erytheropolis, Proceedings of The 12th Iranian biology
Conference.
7- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Alkaline phosphatase activity of Flexibacter
chinensis under starvation stress in strile water microcosoms. 1tst International Conference on
Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005). March
5-18th 2005, Badajoz, Spain
8- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The effect of stress on the protein profiles in
Flexibacter chinensis. 1tst International Conference on Environmental , Industrial and
Applied Microbiology(BioMicroWorld-2005). March 15-18th 2005, Badajoz, Spain.
9- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Heat-Induced Synthesis of δ32 in
F. chinensis: The Role and Function in cell Division . 1tst International Conference
on Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005).
March 15-18th 2005, Badajoz, Spain.
10- Raheb, J., Karkhane, A. and Flint, K.P. (2004). Respiration Activity in Flexibacter chinensis
under Starvation Stress 1tst International Conference on Environmental , Industrial and
Applied Microbiology(BioMicroWorld-2005). March 15-18th 2005, Badajoz, Spain.
11- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The mutation of the ropS gene, the central
regulator of stationary phase, affects the cell division in F. chinensis 1tst International
Conference Environmental Industrial and Applied Microbiology(BioMicroWorld-2005). March
15-18th 2005, Badajoz, Spain.
12- Raheb, J., Karkhane, A. and Flint, K.P. (2004). The effect of environmental factors on
the survival characteristics of Flexibacter chinensis. 1tst International Conference
on Environmental , Industrial and Applied Microbiology(BioMicroWorld-2005).
March 15-18th 2005, Badajoz, Spain.
13- Raheb, J., Aghaei, E., Khaniki, GH.. Ghorbanly, M. and Naghdi, S. (2005). Identification
and Cloning of Oxidoreductase gene from Rhodococcus erythropolis in E.coli .
The 4th National Biotechnology Congress Islamic Republic of Iran, 15-17 Auguest 2005,
Kerman (Mahan).
14- Nazari. B., Haghbeen. K., Raheb, J., Arab Najafi. S. and Fatemi, S.S. (2005).
Optimization Cholesterol Oxidase production by Rhodococcus erythropolis ATCC 4277
by using Taguchi Statistical Nethod. The 4th National Biotechnology Congress Islamic
Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
15- Behzadi. H., Raheb, J., Aghaei, E. and Akbarzadeh, S. (2005). Cloning and Expression of
Oxidoreductase gene from Rhodococcus erythropolis IGTS8 for Enhancement of Biological
Desulfurization. The 4th National Biotechnology Congress Islamic Republic of Iran,
15-17 Auguest 2005, Kerman (Mahan).
16- Mohaghegh. A., Raheb, J., Khaniki, GH.. Akbari, M., Shabani, A and Aghaei, E., (2005).
Cloning of Glucose oxidase Aspergillus Niger in Prokariotic Host. The 4th National
Biotechnology Congress Islamic Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
17- Ghaffari. S., Raheb, J., Akbarzadeh, S. and Ebrahimi, GH. (2005). Desulfurization Process
of Pesudomonas aeroginosa in presence of Biosurfactant . The 4th National Biotechnology
Congress Islamic Republic of Iran, 15-17 Auguest 2005, Kerman (Mahan).
18- Behzadi. H., Raheb, J., Aghayemoghadam, E. and Akbarzadeh, S. (2005). Increasing The
copy Number of Oxidoreductase gene for Importante the Expression of Desulfurization
Genes. 13th Iranian biology Conference and the first International Conference of Biology
Guilan University, Rasht, I.R.Iran, 23-25 august 2005.
19- Raheb, J., Mohaghegh. A., Akbari, M., Shabani, A., Khodabandeh, M and Karkhanei,
A. (2005). Cloning and Expression of Glucose Oxidase Aspergillus niger in E.coli.
13th Iranian biology Conference and the first International Conference of Biology Guilan
University, Rasht, I.R.Iran, 23-25 august 2005.
20- Rasehk, B., Mosavi, MT. and Raheb, J.(2006). Investigation of Biodesulfurization operon
activity in the Rhodococcus Erytheropolis IGTS8 influence of Oxidoreductase gene.
14th National and 2th InternationalConfrence of Biology Tarbiat Modares University,
Tehran, Iran, 2006.
21- Yektamaram, L., Raheb, J., Akbari, Shaabani, A., M and Aghaei, E.(2004), Isolation
and cloning of Glocose oxidase gene from Aspergillus niger In Ecoli. The 3th National
Biotechnology Congress Islamic Republic of Iran, 11-13 September 2004, Mashhad
21- Akbarzadeh, A., Raheb, J.,(2004),Determination of genetic map and Nucleotide sequences
of dszAB in Rhodococcus FMF a native bacteria in IRAN. The 3th National Biotechnology
Congress Islamic Republic of Iran, 11-13 September 2004, Mashhad
23- Jamshid Raheb; Hasan khoshroo; Ali Azimi; Bahram Nasernejad; Mohammad
hossein Sanati; Mahmood Arabnezhad (2007). Isolation and Characterization of a new
Acidothiobacillus Ferrooxidans from the Aliabad copper mine in Yazd using 16s-23s
spacer gene nucleotide sequencing method. II International Conference Environmental
Industrial and Applied Microbiology(BioMicroWorld-2007). 28 November -1December 2007,
Seville , Spain.
24- Jamshid Raheb; Hasan khoshroo; Ali Azimi; Bahram Nasernejad; Mohammad
hossein Sanati; Mahmood Arabnezhad (2007). Isolation and characterization of novel
fastidious, acidophilic species from the Aliabad copper mine in Yazd using 16s-23s
intergenic, spacer gene nucleotide sequencing method . II International Conference
Environmental Industrial and Applied Microbiology(BioMicroWorld-2007). 28 November –
1December 2007, Seville , Spain.
Gene Submission 1- AJ514873.3
2- AJ514949.2
3- AJ514947.2
4- AJ514948.1
5- AJ973326.1
6- AJ973325.1
7- AJ973323.1
8- AJ973324.1
9- AM039687.1
10- AM039688.1
11- AM296495
12- AM296496
13- AM296497
14- AM296498
15- AM296499
16- AM404265
17- AM404266
18- AM404267
19 - AM404268
20- AM404269
21- AM404270
22- AM404271
23- AM404272
24- AM404273
25- AM404274
26- FM946026
27- FM946027
Patents
1- 38302003
2- 38302004
3- 38302005
4- 38303079
1- Khosrow Adeli
Dept of Pediatric
Laboratory Medicine professor
University Of Toronto
555 University Ave
Toronto, Ontario
Canada M5G 1X8
Email: khosro.adeli@sickkids.ca
Tel: (416) 813 8682
Pager: (416) 232 4150
Fax: (416) 813 6257
2- Hojatollah Vali Ph.D.
Director
Faculty for Electron Microscopy Research
McGill University
3640 University Street
Montreal, OC H3A 2B2
Email: hojatollah.vali@mcgill.ca
www.medicine.mcgill.ca /femr
Tel: (514) 398 3025
Fax: (514) 398 5047
3- Nasser Khodai Booran Ph. D
The Hospital for Sick Children
Division Of Infection Diseases
Researcher
555 University Ave
Toronto, Ontario
Canada M5G 1X8
Email: nkhodaibooran@yahoo.com
Nasser.khodaibooran@sickkids.ca
Tel: (416) 813 6009
Pager: (416) 232 4150
Fax: (416) 813 5993
Assistant Secretary of State for Economic, Energy and Business Affairs Jose Fernandez delivers remarks on "The Role of Biotechnology in Advancing African Agriculture,” at Chesapeake Farms in Maryland on September 14, 2011. [State Department photo/ Public Domain]
Isaiah Obara, a PhD graduate fellow researching 'T. parva genetic diversity in relation the MHC diversity of the cattle host' from January 2011 through November 2013, is located on the ILRI-Nairobi campus and supervised by ILRI's Steve Kemp and Richard Bishop (photo credit: ILRI).
15 February 2016, Rome, Italy - Gunter Pauli, Founder, Zero Emissions Research and Initiatives Network, Japan. FAO International Symposium on “The Role of Agricultural Biotechnologies in Sustainable Food Systems and Nutrition”, Opening Session. FAO headquarters (Green room).
Photo credit must be given: ©FAO/Giuseppe Carotenuto. Editorial use only. Copyright ©FAO.
Over the next five years, we will witness surge in approval of new drugs that would deliver a major improvement in the standard of care. www.bharatbook.com
Freshmen Michele San, 14, of Freehold Township and Danielle Granata, 14, of Eatontown analyze a mock crime scene during Intro to Chemistry and Forensics class at Biotechnology High School. ((STAFF PHOTO: TANYA BREEN))
Article Link:
www.app.com/article/20091029/LIFE/910290320/Students-inve...
if you are interested to copy this picture for publication, please write under the photo : © Novasep-Henogen SA photo by Ary
Thank you !
Els ponents de la vuitena sessió de Lessons Learned organitzada per CataloniaBio i Biocat: Miguel Ángel Mayo (Gestha), Diego Rodríguez (J&A Garrigues), Xavier Castells (Inkemia IUCT Group), Anna Rossell (IMB Grup), Inmaculada Torés (EQA), Ignasi Belda (Intelligent Pharma)
Designed for area high school teachers and conducted by JCC biology faculty. Hands-on activities included advanced lab methods and technical skills in cell culture, immunofluorescence, and DNA isolation. August 2011
Students in Dr Jolene Schuster's Biotechnology Class/Lab made a series of protein samples and then measured the amount of UV light that each absorbed using a spectrophotometer. They used the data to create a standard curve relating concentration and absorbance. The curve will be a useful tool for exploring other protein samples they are studying; Gilder Center, April 22, 2022. Photography by Glenn Minshall.
Jamestown Community College hosted an intensive five-week boot camp for high school teachers in the HURI SURI program offered through JCC’s biotechnology degree program. HURI SURI provides undergraduate research opportunities for JCC and area high school students as well as opportunities for teachers to participate in biotechnology and undergraduate research experiences at JCC.
Designed for area high school teachers and conducted by JCC biology faculty. Hands-on activities included advanced lab methods and technical skills in cell culture, immunofluorescence, and DNA isolation. August 2011
An overview of the cafeteria at Biotechnology High School. Principal Linda Eno credits the school's tidy environment to the cooperation of students, faculty and an outstanding janitorial staff. (STAFF PHOTO: TANYA BREEN)
Article Link:
www.app.com/article/20091029/LIFE/910290325/From-clean-to...
Intercollegiate Faculty of Biotechnology UG-MUG
Location: ul. Abrahama 58, 80-307 Gdańsk
Architect: Warsztat Architektury Pracownia Autorska Krzysztof Kozłowski
© Piotr Krajewski
Racheal Aye, PhD student with the Contagious Bovine Pleuropneumonia (CBPP) with the Biotech theme at ILRI (photo credit: ILRI/Aye).
15 February 2016, Rome, Italy - Gunter Pauli, Founder, Zero Emissions Research and Initiatives Network, Japan. FAO International Symposium on “The Role of Agricultural Biotechnologies in Sustainable Food Systems and Nutrition”, Opening Session. FAO headquarters (Green room).
Photo credit must be given: ©FAO/Giuseppe Carotenuto. Editorial use only. Copyright ©FAO.
Dr Jolene Schuster's Biotechnology labs have concentrated on DNA study during April, 2021, and she explains the lesson plan as follows: "The ability to make many copies of select regions of DNA is incredibly valuable, not just in the biochemistry lab, but also in medicine, agriculture, forensic science, evolutionary biology, and many other applications. The basic process is called the polymerase chain reaction (PCR.) Biotechnology class used PCR to amplify a short non-coding region of their own 16th chromosome to create a class genetic distribution profile. Students harvested some cells from inside their cheeks, extracted a small amount of DNA, and mixed the DNA with the right components to set up the PCR reaction. The reaction involves repeated copying of a specific target DNA sequence. The PCR products are analyzed for size and relative concentrations after the reaction. This particular non-coding region, the Alu unit at the PV92 locus, has been used to establish relatedness of individuals for many purposes." Photography by Glenn Minshall.
Dr Jolene Schuster's Biotechnology labs have concentrated on DNA study during April, 2021, and she explains the lesson plan as follows: "The ability to make many copies of select regions of DNA is incredibly valuable, not just in the biochemistry lab, but also in medicine, agriculture, forensic science, evolutionary biology, and many other applications. The basic process is called the polymerase chain reaction (PCR.) Biotechnology class used PCR to amplify a short non-coding region of their own 16th chromosome to create a class genetic distribution profile. Students harvested some cells from inside their cheeks, extracted a small amount of DNA, and mixed the DNA with the right components to set up the PCR reaction. The reaction involves repeated copying of a specific target DNA sequence. The PCR products are analyzed for size and relative concentrations after the reaction. This particular non-coding region, the Alu unit at the PV92 locus, has been used to establish relatedness of individuals for many purposes." Photography by Glenn Minshall.