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Tissue sections on slides are stained using hematoxylin and eosin on an automated stainer. This is the routine "H&E" stain, which is used on almost all specimens. Hematoxylin, which is blue or purple, stains mostly nuclei, while the pink to orange eosin stains cytoplasm, nucleoli, and extracellular substances like collagen.

 

After staining, the processor takes the slides through a dehydrating solvent, usually alcohol or acetone, and then into a "clearing agent," either xylene or a citrus-smelling substance called Americlear.

 

Sometimes it is necessary to supplement the H&E with "special stains" or "immunostains."

@Pathology2018 #histopathology #cytopathology #Liverpathology #Molecularpath #dermpath #pulmonarypath #Diagnosticspath #perinatalpath #experimentalpath #autopsypath #e_pathology #plant #renalpath #immunopath #Digitalpath #nephrpopath #neuropath #SLPeeps

PS:https://pathology.euroscicon.com/

Here's a low power photomicrograph of scalp. The round things are hair follicles.

RD&E Pathology building

 

I think I am right in saying that EVERY sinlge patient that comes through the doors at the RD&E has at least one form of sample enter this building.

 

Then there are of course the countless others form the community.

 

It is a shame that it looks like something out of 1970's Hospital thriller movie (esp at night time)

 

I don't think it gets enough love - so join and send the Path building some <3

Go to Page with image in the Internet Archive

Title: University of Maryland Theses 1873

Publisher:

Sponsor: LYRASIS Members and Sloan Foundation

Contributor: University of Maryland, Baltimore Digital Archive

Date: 1873

Language: eng

This volume contains the following manuscripts described as either an Inaugural Dissertation or an Inaugural Essay presented to the University of Maryland for the Degree of "Doctor of Medicine" or "Doctor of Physic" : General Pathology / by Gayle, V.W. ; Chancre or the Initial Legion of Syphilis / by Winslow, Randolph ; Gunshot Injuries / by Hoen, Adolph G. ; Vaccinia / by Ashby, Thomas ; Degeneratio Cordis Adiposa / by Worthington, J. C. ; Acute Dysentery / by Jones, G. Monroe ; Diphtheria / by Gerstell, Richard ; General Treatment of Typhoid Fever / by Humrichouse, J. W. ; Tubercular Meningitis / by Scholl, Charles E. ; Causes of Abortion / by Winchester, A. J. ; Bronchitis / by Benson, Benjamin R. ; Conservative Surgery / by Gerstell, Robert (No title on cover page) ; Intermittent Fever / by Maxwell, William S. ; Methods of Medical Research / by Toombs, Robert Jr. ; Sulphate of Quinia / by Stansbury, Oscar ; Phthisis Pulmonalis / by Baldwin, Joe S. ; Cholera Infantum / by Miller, James B. ; Inflammation / by Warner, F. A. ; Typhus Fever / by Beard, Stephen ; Hernia / by Clarke, Andrew P. ; Entero-colitis / by Bulluck, David W. ; Etiology / by Rogers, William Hughes ; Acute Articular Rheumatism / by Sadtler, Charles E. ; Diphtheria / by Fairall, T. E. ; Typhus Fever / by Pipino, Will C. ; Report of Six Cases / by Michael, J. Edwin ; Typhoid Fever / by Harrington, J. E. ; Pathology of Inflammation / by Shackelford, J. A. ; Hernia / by Crothers, Ransom R.

 

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