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We have recently established a novel immunohistochemistry-based procedure to detect expression of up to 5 proteins in formalin-fixed paraffin-embedded (FFPE) tissues and cell samples. The procedure is based upon sequential combination of horseradish peroxidase enzyme labelling that is visualised using the diaminobenzidine (DAB) brown or yellow substrates with the alkaline phosphatase enzyme labelling revealed using chromogens of different colours (i.e. Fast Blue, Fast Red, Green).
The present five colours immunostaining of a single section of human tonsil shows expression of CD34 (yellow) in vascular endothelium, CD138 (green) in plasma cells and epithelial cells of tonsillar criptae, CD3 (purple-red) in T-cells, the B-cell associated transcription factor PAX-5 (nuclear, brown) in germinal center B-cells and CD21 (blue) in follicular dendritic cells within a germinal center. Note the absence of non-specific background labelling in the sections and no nuclear hematoxylin counterstain was applied.
The immunostained slide was scanned using the NanoZoomer Digital Pathology System (Hamamatsu). The authors thank Mr D Oukrif for his technical support using the NanoZoomer.
Ayse U Akarca
Dr. Anna Dunnigan will present Speech on "An Analysis of the Causes of Death Identified at Autopsy in the Obese Population" at @Pathology2018
#pathology #histopath #digital_pathology #chemical_pathology #liverpath #breastpath #SLPeeps #pathologists
Lisa Jankov, Assistant Treasurer and Paul Desiri, Manager for the Nuclear Energy division pictured with United Way CEO, Shelley White.
GE Canada was celebrated for receiving the award for the sixth consecutive year.
“GE Canada is committed to working for a stronger Canada. In business they are Building, Powering, Moving and Curing the world. And when it comes to their United Way campaign they are dedicated to ’Live Healthy, Live GEnerously, Live United’”, said Shelley White, United Way President and CEO. “Not only is GE committed to transforming healthcare in Canada through innovations like digital pathology but, to also strengthen Canada’s global competitiveness to build a culture of innovation and collaboration.”
Lisa Jankov, Assistant Treasurer and Paul Desiri, Manager for the Nuclear Energy division pictured with United Way CEO, Shelley White.
GE Canada was celebrated for receiving the award for the sixth consecutive year.
“GE Canada is committed to working for a stronger Canada. In business they are Building, Powering, Moving and Curing the world. And when it comes to their United Way campaign they are dedicated to ’Live Healthy, Live GEnerously, Live United’”, said Shelley White, United Way President and CEO. “Not only is GE committed to transforming healthcare in Canada through innovations like digital pathology but, to also strengthen Canada’s global competitiveness to build a culture of innovation and collaboration.”
Introduction to Digital Pathology
The digital slide workflow
Technical aspects
Image analysis
Novel imaging techniques
Organizational processes to support telepathology services
Introduction to Digital Pathology
The digital slide workflow
Technical aspects
Image analysis
Novel imaging techniques
Organizational processes to support telepathology services
Introduction to Digital Pathology
The digital slide workflow
Technical aspects
Image analysis
Novel imaging techniques
Organizational processes to support telepathology services
Medical Image Understanding and Analysis Conference (MIUA) 2016. Loughbrough University, Leicestershire, UK from the 6th to 8th July 2016
Introduction to Digital Pathology
The digital slide workflow
Technical aspects
Image analysis
Novel imaging techniques
Organizational processes to support telepathology services
Introduction to Digital Pathology
The digital slide workflow
Technical aspects
Image analysis
Novel imaging techniques
Organizational processes to support telepathology services
Mass spectrometry imaging (MSI) is a powerful analytical technique with which one can acquire a map of chemicals in biological samples. Combining this technique with traditional histology, which is a type of tissue-staining procedure done in medical labs to visualize tissue components, can provide complementary information by linking the tissues’ structure to its chemical makeup. However, improving the accuracy of image overlays is crucial for better image clarity down to the single-cell level, which is essential for digital pathology. Our photo is a mouse brain tissue collected on a glass slide that was laser-etched with fiducial markers (crosses), analyzed using MSI (one-third of whole tissue) and stained with hematoxylin and eosin dye post-MSI. Results indicated that the fiducial markers provided significantly better alignment and accuracy. This research demonstrates the feasibility and benefits of integrating MSI with optical imaging, marking progress towards improved diagnostic capabilities in digital pathology.
Ameh Orotomah (Department of Chemistry and Biochemistry)
OptraSCAN (www.optrascan.com) for research-use-only, is the first On-Demand compact Digital Pathology system to provide a comprehensive, affordable end-to-end Digital Pathology solution.
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