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Phylogenetic pattern and N-linked glycosylation of a subset of Ciona HCNs.A. An alignment of the pore region produced by ClustalX and generated by GeneDoc. Red boxes indicates known and putative HCN N-linked glycosylation site and the yellow line indicates the division between the presence and absence of this functional site. B. An HCN cladogram generated by aligning the sequences shown as described in the text. ?X? indicates the predicted emergence point of N-linked glycosylation in the HCN family. Sequences other than those in Figure 1, and other than those for Oikopleura dioica and Branchiostoma floridae (lancelet), are: gi: 260829977; Strongylocentrotus purpuratus (sea urchin) gi:47551101; Panulirus interruptus, splice variant I (lobster) gi:115334851; Apis mellifera (bee) gi:33355927; Drosophila melanogaster, isoform B, gi:84795752; Aedes aegypti (mosquito) gi:108875949. C. Western blot of membrane fractions from oocytes injected with cRNA of ciHCNa, ciHCNa-N380Q or ciHCNb, untreated (?) or treated (+) with PNGaseF and probed with anti-V5 antibody. Actin, robed with an anti-actin antibody, was used as a loading control. ciHCNa, but not ciHCNa-N380Q or ciHCNb, is shifted to a lower molecular mass in the presence of PNGaseF. Molecular weights, indicated by the short black bars to the right of the blots, are 130 kDa (top), 100 kDa (upper middle) and 70 kDa (lower middle) and 35 kDa (bottom). D. Western blot of whole cell lysates from CHO cells transfected with 1.5 ug cDNA of ciHCNa, ciHCNa-N380Q or ciHCNb, either treated (+) or untreated (?) with PNGaseF and probed for using an anti-V5 antibody. Molecular weights, indicated by the short black bars to the right of the blots, are 95 kDa (top), 72 kDa (middle) and 34 kDa (bottom). Predicted mass of Ciona HCN channels without post-translational modification, is 78.8 kDa and 93.3 kDa for ciHCNa and ciHCNb, respectively.

Taken on Saturday 3rd January 2009 in Fallowfield

modern marble cladding Graffiti for outdoor detail visit javabali.info

Grace Community Church gymnasium with gray fiber cement boards on the interior walls.

Bold, colourful cladding enhanced by winter sun at the Arch Street apartments development, near Elephant & Castle, London Borough of Southwark. Soon to be occupied (Feb 2011), developer L&Q Housing Association.

 

©2011 Images George Rex. All Rights Reserved.

Commercial Cladding, Eva-tech® Savanna (C19) Rivonia

6 6 6

simply ugly and scary

More often I walk past. But I never take a picture. This time I take it up.

Much more scary with the correct background: On Black

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Almost american,made even more so by its neighbour being retro fitted with Clapboard.

White Fiber Cement Boards. Designed by DLR Group.

A 1920s gem, even better for the thick use of yellow in addition to the more usual dark red and grey. One of my favourites, with interestingly angled garden path. The wall looks original. Aluminium window and door frames complete the confection.

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Anodized aluminium tiles form a portholes-accommodating tessellation using 3 different shapes as indicated. The whole building will be clad like this, which promises to be striking and perhaps spectacular. Peninsula Square, North Greenwich. Architects: FOA. Jan 2010.

 

Image: Copyright ©2010 George Rex Photography.

Product Used: Terracotta Wall Cladding

Size: 12 X 4 X 2

Profile-6 Corrugated Fiber Cement Boards on an office building in Santa Cruz, CA. Architect: The Envirotects.

Custom home with Profile-6 corrugated fiber cement boards. Designed by Faye and Walker Architects. Photo by Leonid Furmansky

Profile-6 Corrugated Fiber Cement Boards on an office building in Santa Cruz, CA. Architect: The Envirotects.

Comparative genomic DNA hybridization and in silico comparison of gene content within mobile elements of bovine and human SA isolates.In the MLST-based cladogram generated by PAUP [74], [76] at the left, “B” denotes a strain originally isolated from a bovine; “H” denotes isolation from a human. All data associated with “B” strains and MSA553A are microarray hybridization data; all remaining “H” strains show the results of in silico Smith-Waterman alignment of the 70mer microarray probe to the genome sequence. Red = absent, yellow = indeterminable/variable, green = present. OPtrans = oligopeptide transporter island and Ebh = region of probes representing the ebh gene or gene remnants+probes for 6 gene insertion in ET3-1. The white boxes within the SaPIbov1 and SaPIbov3 islands show gene clusters that were consistently absent from strains associated with human infection and largely conserved in strains recovered from bovine sources, including those that more closely resemble the isolates from humans.

Old Whittington, Chesterfield. Shot on Kodak film.

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Profile-6 Corrugated Fiber Cement Boards on an office building in Santa Cruz, CA. Architect: The Envirotects.

Grace Community Church gymnasium with gray fiber cement boards on the interior walls.

2 story stainless steel clad penthouse stair. Caliper Studio detailed, fabricated and installed for AVO Construction and Ben Hansen Architects.

There is something amiss in the front gable, beneath the cross (nice touch that) Use of a darker grey is sparing, as is the red. These are fine houses. I cannot agree with the front wall. The softening effect of stone cladding is demanded.

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Profile-6 Corrugated Fiber Cement Boards on an office building in Santa Cruz, CA. Architect: The Envirotects.

Custom home with Profile-6 corrugated fiber cement boards. Designed by Faye and Walker Architects. Photo by Leonid Furmansky

2 story stainless steel clad penthouse stair. Caliper Studio detailed, fabricated and installed for AVO Construction and Ben Hansen Architects.

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Residence Bognor Summerstrand, Infinity® decking, Tiger Cove, Apex® Cladding OJP Himalayan Cedar, LifeSpan™ Pergola, Savanna

Minerit HD fiber cement boards with exterior insulation on a rain-screen.

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