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An FDA laboratory worker injects an influenza virus into an egg, where it will grow before being harvested—one of the many complex steps involved in creating a traditional flu vaccine. To learn more about where the science is going, read this FDA Consumer Update:
• The Evolution, and Revolution, of Flu Vaccines
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Large population studies have identified numerous genes which increase risk of mental illness. The task now is to understand their normal function in the brain and how their malfunction contributes to illness. Here we show cells stained red for the protein produced by a gene, ABCA13, that we have linked to schizophrenia and bipolar disorder (manic depression). The cells are also stained green for a scaffolding protein in the cells and blue for the DNA in the cell nucleus – in both cases to help us orient the structures of the cell. The pattern of ABCA13 distribution in the cell suggests that it might play a role in the transport of substances into and out of the cell.
Images are of the glial cell line U373 stained for a candidate Schizophrenia susceptibility protein, ABCA13 (red) and an alpha-tubulin cytoskeleton marker (green). Image was captured on a Leica SP5 confocal microscope equipped with HyD detectors.
food-lipidomics-in-nutrition-and-human-health-Lipids are metabolites of an individual's diet and metabolic pathways. Accurate measurement of all lipids within a single biological fluid, tissue, or cell type through MS‐based lipidomics can effectively establish the association between diet and health.
analysis of protein ubiquitination-Ubiquitination analysis is a key biochemical technology for the development of new drugs and new targets. This method can be used to detect ubiquitinated proteins; determine the ubiquitination process of these proteins; identify specific sites where lysine residues are ubiquitinated; and also be used for quantitative analysis of ubiquitination protein.
Glycosylation Analysis-Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including the attachment of cell to the extracellular matrix and intracellular protein-ligand interactions. This PTM is characterized by various glycosidic linkages, including N-, O- and C-linked glycosylation, glypiation (GPI anchor attachment), and phosphoglycosylation, etc.
The Americas holds the largest share of the global #ImmortalizedCellLine market, would reach USD 1,505.38 Million by 2023. Get Market Forecast 2023 - www.aarkstore.com/healthcare/979874/global-immortalized-c... #CellLineMarketAnalysis #Health #StemCellTherapy #CellLines #CancerTherapy #MarketResearch #MarketReports
The cell ca Host Cell Line The CHO DG44 cell line has been modified to serum-free, chemically defined cell culture media and suspension growth. The resultant host cell line is metabolically optimized for stable growth over longer periods, making it ideal for meeting the large-scale requirements for protein look and genetic stability.
www.genscriptprobio.com/add-procld-cell-line-development-...
Author: Aylin Özdemir Bahadır
Abstract: In the pharmaceutical industry, biopharmaceuticals (biologics) are gaining market share. There has been a dramatic increase in the sale and market penetration of monoclonal antibodies in particular. Typically, therapeutic antibodies are produced using high-expression, clonal, or recombinant CHO cell lines. CHO cells dominate the market as a commercial production host due to their ease of use, built-in regulatory records, and security profiles. While traditional limiting-dilution and cloning-ring regulations are frequently used to select mammalian cell lines that produce high levels of proteins, they have a number of drawbacks. ClonePix2 is a fully automated, single cell-based clone selector that significantly increases the likelihood of rapidly selecting high-production clones with high monoclonality. Scfv-Fc recombinant antibody structures with a variety of therapeutic advantages have gained prominence in recent years. Single cell cloning of CHO cells expressing the scfv-Fc fusion protein, which differs from the classical immunoglobulin structure, was performed in situ using the ClonePix2 device using FITC-tagged anti-Fc and anti-H+L antibodies. The fluorescent intensity parameters of the resulting cell clones were analyzed. Additionally, ELISA was used to determine the production capacities of the best clones. As a result, it was established that anti-Fc antibody recognizes the scfv-Fc fusion protein in a semi-solid environment, enabling the identification of higher production clones.
Keywords: Biopharmaceuticals, cell-line, high-producer, scfv-Fc antibody, ClonePix2