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Lens: Laowa 25mm 2.5x-5x ultra macro @ 5x @ f3.4

Number of shots: 68 @ 40um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Camera: Nikon D850

Lens: Laowa 25mm 2.5x-5x @5x @ f3.4

Number of shots: 87 @ 40um stepsize, manual controlled

Illumination: 4 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Staged setup

Zweefvlieg.

 

Zerene stack Pmax.

50 steps @ 80 um.

Cycle capture time 1.2 sec/picture.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 2X.

 

FG led flash module 10 channels

Direction light D 3.0

Lighting direction profile default.

Total led power 64% of 500W = 320W.

Flash time: 1.5 msec.for 100% power.

 

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 128 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Hoverfly.

I removed most of the pollen from the eye under the binocular stereo microscope before taking the picture. This task took about an hour!. The rest I leave, there are hundreds of such pollen. Remarkable are also the small white hairs on the eye facets.

Dansmug - Chironomidae.

 

Zerene stack Pmax.

229 steps @ 7 um.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 10X.

 

FOV: 2.3 mm.

 

FG led flash module 10 channels

Direction light D 5.3

Lighting direction profile default.

Total power 64%

Flash time: 1.5 msec.for 100% power.

 

Just one additional test with the direction of the flash exposure. This is a 100% crop. A full version see:

www.flickr.com/photos/fotoopa_hs/29243578675/

  

Bladluis - Aphidoidea .

FOV: 1.9 mm

 

Zerene stack Pmax.

193 steps @ 7 um.

Cycle capture time 1.2 sec/picture.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 10X.

 

FG led flash module 10 channels

Lighting direction profile default.

Total led power 64% of 500W = 320W.

Flash time: 1.8 msec.for 100% power.

 

Setup see: www.flickr.com/photos/fotoopa_hs/28856108781/

 

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4

Number of shots: 214 @ 25um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged (but the prey was really its prey)

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 5x @ f3.4

Number of shots: 130 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 1 row of 3 photos each of approx. 250 shots @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Length of insect: approx 12mm

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 5x @ f3.4

Number of shots: 236 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Subject: Tiny moth

Camera: Nikon D850

Crop: 1.5x (DX-mode)

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4

Sigma 1.4x Teleconverter

Total magnification: approx. 10x

Number of shots: 85 @ 30um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Length of insect: approx. 11mm

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 3.5x @ f3.4

Number of shots: 160 @ 25um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 5x @ f3.4 + 56mm Extension rings

Magnification: approx 7x

Number of shots: 166 @ 15um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Body length: approx. 3mm

Specimen: probably male Clubiona subtilis

Lens: Nikon CFI Plan Achromat 10x NA 0.25 on Raynox DCR 150 used as a tube lens

Number of shots: 406 @ 5um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100 + background LED-panel, custom tracing paper-based diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 2.5x

Number of shots: 121 @ approx. 40um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

The most remarkable feature of this insect are the 7 eyes: 3 simple eyes (ocelli), 2 compound eyes, and 2 turbinate eyes (orange) used to distinguish females in a swarm of mayflies.

Canon EOS M, JML Optical 21mm f/3.5. Stack of 115 shots.

 

Lighted by the Hexa-Lite Mk IV, my latest incarnation of a DIY powerLED Ringlight.( work in progress)

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 3.5x @ f3.4

Number of shots: 169 @ 35um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Dried sugar solution

Lens: Nikon CFI Plan Achromat 10x NA 0.25 on Raynox DCR 150 used as a tube lens and a cross-polarisation filter (two small linear polarisation sheets)

Number of shots: 81 @ 5um stepsize using an MJKZZ Qool Rail 250

Illumination: 1 x Ulanzi L1 powerled (transmitted lighting)

Edit: Helicon focus, Photoshop CC, Lightroom C

Body length: approx 5 mm

Lens: Laowa 25mm 2.5x-5x @ 4x @ f3.4

Number of shots: 169 @ 25um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser, background illumination with color gel and grid.

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Length of insect: approx 12mm

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4

Number of shots: 177 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

The moth of the previous photo has now dried out during a couple of days. The structure and coloration of the eyes have changed significantly.

 

Body length: approx. 11mm

Lens: Nikon CFI Plan Achromat 10x NA 0.25 on Raynox DCR 150 used as a tube lens

Number of shots: 390 @ 5um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom tracing paper-based diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Body length: approx 5.5 mm

Lens: Laowa 25mm 2.5x-5x @ 3.5x @ f3.4

Number of shots: 101 @ 40um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser, background illumination with color gel and grid.

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: S&H 20x 0,35 (microscope lens)

Number of shots: 186 @ approx. 6um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Hoverfly.

 

Zerene stack Pmax.

62steps @ 50 um.

Nikon D7100 + AFD 200 F7.1 ISO 200

Mitutoyo M Plan APO 2X.

 

FG led module flash time: 7.2 msec 6800 lumen.

 

Update 2012:

 

There is a new version 2012 with beamsplitter:

www.flickr.com/photos/fotoopa_hs/6760414583/

 

And of course the first results:

www.flickr.com/photos/fotoopa_hs/sets/72157629073800140/

 

High speed macro 3D portable unit with optical extern focus detector using 2 DSLR camera's and front side type mirrors.

 

First pictures of the portable 3D setup. The picture show only the mechanics parts. The electronic box need to added into the next days. This unit is realy difficult to make. There are a lot of fine pices to calibrate. The 2 camera's D300 and D200 are inline and can be adjusted direction mirrors. This is needed because the AF105/2.8D macro lenses d'nt have a fix lenght. The mirrors can be moved into the other direction on a slide. On top of this slide there are the 2 mirrors. They can turn a little to adjust for the keystone effect and to center the pictures on the frame. At the bottom on each side the IR lasers are mounted. The IR lasers can turn into X-Y-Z direction and are finetuned via separate screws. On the top of the unit the AF60/2.8D macro lens record the reflected IR lasers signal from the objects infocus. Into the body of this macro lens there is the AVR controller circuit to measure this IR light. This information goes to the central hardware controller for further action.

The 2 mirrors are protected for most of dust via the 2 nikon 62mm diameter filters. Just near this filters there are 2 powerleds to work at night. The 2 flashes are on the top and on both side there are the green lasers. The green lasers are used for me to point the unit to the objects. Even in full sunlight is see this green pointers. They are crossed mounted into the ideal focuspoint. The IR lasers are also crossed on this same point.

 

On the otherside I have mounted an optional calibration slide tool. See:

www.flickr.com/photos/fotoopa_hs/4351166539/

 

The testcard stay on this slide at the correct position and can be moved forwards and backwards. The textcard is used to calibrate the hole unit and to adjust them for the desired frame format. Moving the testcard d'nt change the high of the object, so this is a very helpfull tool to adjust and calibrate the hole unit.

 

The weight of the unit is near 8.5kg inclusief the electronics parts. This is very heavy to carry into the field but the results of this hightech detection system and 3D are so promising. I try now to cut down as many as possible alu parts to lower the wight. But the unit must stay stable. The load into the field is very heavy for all the parts.

 

Update 22 March 2010:

The setup is now ready and works perfect. Frame size is set at 100mm horizontal. Flash powers set in TTL mode at nominal value of 1/32 power. Pictures are taken at F29, ISO200, T=1/250. Burstmode set at max 3 pictures/second. The IR laser detection works perfect, need 40usec for the detection cycle. The detection signal goes from 10 units (black object) to 50 units (white object). The triggerlevel stay at 3 units, so all levels above this 3 units will give a trigger. I d'nt have an external shutter on this 3D version. The shutterlag from the 2 different camera's are 53.5 msec (D300) and 58 msec (D200). The controller synchronize this 2 camera's, first the D200 goes on and 58-53.5=4.5 msec later the D300. On the 58msec delay the flashes are fired. This time stay very constant over the time, all pictures are well lighted.

 

The first results from 2010:

www.flickr.com/photos/fotoopa_hs/sets/72157623127172132/

 

There is a new version 2012 with beamsplitter:

www.flickr.com/photos/fotoopa_hs/6760414583/

 

And of course the first results:

www.flickr.com/photos/fotoopa_hs/sets/72157629073800140/

 

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 117@ 20um stepsize using a MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 1 rows of 4 photos each 166 shots @ 25um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Rove beetle.

 

Zerene stack Pmax.

108 steps @ 20 um.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 5X.

 

FG led flash module 10 channels, flash time: 3 msec.

Preset 2, Direction light D5

 

See this new flash led module:

www.flickr.com/photos/fotoopa_hs/28080817403/

View in my mouth. Okay I still have to brush my teeth!

 

Zerene stack Pmax.

175steps @ 18 um.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 5X.

 

FG led flash module 10 channels

Direction light D1.1

Lighting direction profile default.

Total power 64%

Flash time: 2 msec.for 100% power.

 

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4 + 56mm extension tube

Total magnification: approx. 7x

Number of shots: 143 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4 + 56mm extension tube

Total magnification: approx. 7x

Number of shots: 236 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 3x @ f3.4

Number of shots: 189 @ 25um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Body length: approx. 9mm

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 3.7x @ f3.4

Number of shots: 261 @ 30um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom tracing paper-based diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 157@ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 161 @ 40um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Length of insect: approx 2mm

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 5x @ f3.4 + 56mm extension rings

Magnification: approx 7x

Number of shots: 116 @ 15um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Dansmug - Chironomidae.

 

Zerene stack Pmax.

124 steps @ 7 um.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 10X.

 

FOV: 1.7 mm.

 

FG led flash module 10 channels

Direction light D 4.0

Lighting direction profile P3.

Total power 22%

Flash time: 3.4 msec.for 100% power.

 

Detail of the previous harvestmen photo at 10x magnification.

 

Lens: Nikon CFI Plan Achromat 10x NA 0.25 on Raynox DCR 150 used as a tube lens

Number of shots: 339 @ 5um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom tracing paper-based diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4 + Sigma 1.4 Teleconverter

Total magnification: approx. 7x

Number of shots: 105 @ 30um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, Custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5x-5x @ 5x @ f3.4

Number of shots: 83 @ 40um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Bandzweefvlieg - Epistrophe.

Correction: Close-up 3 extra big eyes into my eyes ..

more close-up see:

www.flickr.com/photos/fotoopa_hs/28755305484

 

Zerene stack Pmax.

142 steps @ 18 um.

Nikon D7100 + AFD 200 F5.6 ISO 200

Mitutoyo M Plan APO 5X.

 

FG led flash module 10 channels

Direction light D3.1

 

Lighting direction profile manual, power per channel in %:

LD1: 96

LD2: 96

LD3:32

LD4:00

LD5:00

LD6:00

LD7:00

LD8:00

LD9:00

LD10:32

Flash time: 3.2 msec.for 100% power

 

See this new flash led module:

www.flickr.com/photos/fotoopa_hs/28080817403/

 

The full setup now:

www.flickr.com/photos/fotoopa_hs/28856108781/

  

Body length: approx. 3mm

Lens: Venus Optics Laowa 25mm 2.5x-5x @ 3.5x @ f3.4

Number of shots: 221 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom tracing paper-based diffuser

Edit: Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: S&H 20x 0,35 (microscope lens)

Number of shots: 175 @ approx. 6um stepsize, manual controlled

Illumination: 4 Ulanzi L1 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Lens: Laowa 25mm 2.5-5x ultra macro @ 5x @ f3.4

Number of shots: 204 @ 20um stepsize using an MJKZZ Qool Rail 250

Illumination: 4 Ulanzi L1 powerleds + Ulanzi VL100, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Scene: Staged

Wasp width including wings is only 1.6mm.

Wasp head is slightly below the glue. The ultra small dimensions were extremely difficult to fix the wasp. Also, the legs are slightly under the glue. This is my smallest ever recorded wasp and I have no idea what kind of wasp.

 

Zerene stack Pmax.

150 steps @ 6 um.

Nikon D7100 + AFD 200 F7.1 ISO 200

Mitutoyo M Plan APO 10X.

 

FG led module flash time: 7.2 msec power 6800 lumen.

 

Zandbij.

 

Zerene stack Pmax.

82 steps @ 18 um.

Nikon D7100 + AFD 200 F7.1 ISO 200

Mitutoyo M Plan APO 5X.

 

FG led module flash time: 8.2 msec.

 

Camera: Nikon D850

Lens: Laowa 25mm 2.5x-5x @5x @ f3.4

Number of shots: 161 @ 40um stepsize, manual controlled

Illumination: 4 powerleds, custom diffuser

Edit: Lightroom C, Helicon focus, Photoshop CC, Lightroom C

Staged setup

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