Back to photostream

The Spinning disk’s Darkness by Kevin Richetin, University of Lausanne

Entry in category 3. Locations and instruments; Copyright: CC-BY-NC-ND: Kevin Richetin

 

Long exposure photography of live imaging experiment with spinning Disk confocal microscope (Nikon TiE). Fluorescence images of neurons in acute section of mouse brain were captured every 30s in a chamber at 37°C with 5% CO2 during 3 hours. During this period, the investigator must remain in the darkness in order to monitor the behavior of specific cell infected with lentiviral vector coding for mitochondrial biosensor. This innovative technology permits to investigate specific question as the mitochondrial redox state of mitochondria during stress or pathologies.

 

Spinning Disk confocal microscope is a powerful tool to monitor the functioning of adult brain. In this picture, investigator must remain 3 hours in the darkness to understand the complexity of neuronal dysfunction in the brain of mouse with age-related dementia.

1,245 views
0 faves
0 comments
Uploaded on April 17, 2018
Taken on January 24, 2018