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Microscope Calibration

Image of a ruler with 10ths of an inch numbered, and hash marks for 100ths of an inch. This shows just over 7 of the hundreths marks.

 

National Microscope Model 163, with 4x objective and Canon T2i camera mounted on a 2.5x camera adapter.

 

The 4x objective without the camera has a claimed 4.5 mm field of view. The eyepiece shows .18" across = 4.5mm.

 

The camera image shows about .075", or about 1.9mm, which shows the 2.5x magnification of the photo adapter.

 

Without the adapter it is a 4.5mm field of view, 40x magnification. So I think the camera adapter makes that 100x magnifiation?

 

But the key is pixels per mm...

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Uploaded on April 26, 2010
Taken on April 26, 2010