CRISPR/Cas9 in Neurons

CRISPR/Cas9 engineering was used in mouse embryonic stem cells to insert a GFP tag in frame with the motor-neuron-specific transcription factor HB9. These cells were differentiated into motor neurons. The resulting motor neuron nuclei are labeled with the GFP reporter (green) and counterstained with antibodies against the neuronal marker Tuj1 (red). Credit: T. Macfarlan, NICHD

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