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nih-aligned-coin-Output

Image stack processed with Image-J; stack aligned with StackReg and then focus stacked with Extended Depth of Field. Compare with the same thing done with Helicon Focus. Oh, in this one I didn't clean up all the nicely focussed dust ...

 

Algorithm credit:

B. Forster, D. Van De Ville, J. Berent, D. Sage, M. Unser, " Complex Wavelets for Extended Depth-of-Field: A New Method for the Fusion of Multichannel Microscopy Images," Microscopy Research and Technique, vol. 65, no. 1-2, pp. 33-42, September 2004.

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Uploaded on March 18, 2008
Taken on March 18, 2008