International Journal of Life Sciences and Biotech
Identification of Drought-Responsive Genes in Turkish Bread Wheat (T. aestivum L.) Cultivar Gerek 79 by mRNA Differential Display Analysis
Authors: Diğdem Aktopraklıgil Aksu, Abdulrezzak Memon
Abstract: Wheat is one of the most important cereal crops in the world. Many parts of the world depend on wheat as a source of food and animal feed. Drought stress negatively affects its development and greatly reduces its production. Drought response is a complex genetic mechanism involving multiple genes, transcription factors, miRNAs, hormones, proteins, co-factors, ions, and metabolites. Understanding the genetic basis of the drought tolerance mechanisms is very important for genetic improvement of this trait in wheat. Wheat is also an important cereal crop in Turkey. In this study, it is aimed to identify drought stress-regulated genes in bread wheat (Triticum aestivum cv. Gerek 79) and gene expression profiling using mRNA differential display (mRNA DD) was performed for seedling leaves of control and drought-stressed plants (62.4% of RWC). The comparative profiling study showed a total of 20 differentially- expressed cDNA bands and 10 of them were cloned and sequenced. The inserts with poor quality reads were eliminated. The nucleotide sequences of the remaining two cDNAs named G1 and G2 were subjected to similarity analysis. G1 and G2 showed a high degree of homology the mRNA of purple acid phosphatase and glycosyltransferase family 92 protein-like sequences of Triticum aestivum and some other plants, respectively. Purple acid phosphatases have been shown to be involved in plant responses to abiotic and biotic stresses. Similarly, the role of glycosyltransferases in thermotolerance has been reported in rice besides their functions in cellular homeostasis and detoxification pathways in plants. These reports and our findings have laid a foundation for further investigation of G1 and G2 cDNA clones. The investigation of differential expression of these gene fragments corresponding to purple acid phosphatase and glycosyltransferase family 92 protein-like sequences under drought conditions at the RNA level is ongoing. Further characterization of these genes could be important in understanding the functions of these gene/s in drought response.
Keywords: Wheat, drought, mRNA differential display, gene expression
dergipark.org.tr/tr/download/article-file/3166761
Identification of Drought-Responsive Genes in Turkish Bread Wheat (T. aestivum L.) Cultivar Gerek 79 by mRNA Differential Display Analysis
Authors: Diğdem Aktopraklıgil Aksu, Abdulrezzak Memon
Abstract: Wheat is one of the most important cereal crops in the world. Many parts of the world depend on wheat as a source of food and animal feed. Drought stress negatively affects its development and greatly reduces its production. Drought response is a complex genetic mechanism involving multiple genes, transcription factors, miRNAs, hormones, proteins, co-factors, ions, and metabolites. Understanding the genetic basis of the drought tolerance mechanisms is very important for genetic improvement of this trait in wheat. Wheat is also an important cereal crop in Turkey. In this study, it is aimed to identify drought stress-regulated genes in bread wheat (Triticum aestivum cv. Gerek 79) and gene expression profiling using mRNA differential display (mRNA DD) was performed for seedling leaves of control and drought-stressed plants (62.4% of RWC). The comparative profiling study showed a total of 20 differentially- expressed cDNA bands and 10 of them were cloned and sequenced. The inserts with poor quality reads were eliminated. The nucleotide sequences of the remaining two cDNAs named G1 and G2 were subjected to similarity analysis. G1 and G2 showed a high degree of homology the mRNA of purple acid phosphatase and glycosyltransferase family 92 protein-like sequences of Triticum aestivum and some other plants, respectively. Purple acid phosphatases have been shown to be involved in plant responses to abiotic and biotic stresses. Similarly, the role of glycosyltransferases in thermotolerance has been reported in rice besides their functions in cellular homeostasis and detoxification pathways in plants. These reports and our findings have laid a foundation for further investigation of G1 and G2 cDNA clones. The investigation of differential expression of these gene fragments corresponding to purple acid phosphatase and glycosyltransferase family 92 protein-like sequences under drought conditions at the RNA level is ongoing. Further characterization of these genes could be important in understanding the functions of these gene/s in drought response.
Keywords: Wheat, drought, mRNA differential display, gene expression
dergipark.org.tr/tr/download/article-file/3166761