International Journal of Life Sciences and Biotech
Antifungal activity and optimization procedure of silver nanoparticles green synthesized with Prunus laurocerasus L. (cherry laurel) leaf extract
Authors: Ugur Yigit, Yaren Gurel, Hasan Ilhan, Muharrem Turkkan
Abstract: In this study, green synthesis conditions of silver nanoparticles (AgNPs) synthesized with Prunus laurocerasus (cherry laurel) leaf extract as reducing and coating agent were optimized using Box-Behnken design (BBD). Three important synthesis factors such as the concentration (M) of silver nitrate (AgNO3), pH of cherry laurel leaf extract, and reaction temperature (°C) were used as independent variables of the model, and the absorbance intensity originating from AgNPs was employed as a dependent variable. Statistical analyzes showed that the optimized conditions for the predicted absorbance at 405 nm (2.35 A.U) were determined at a concentration of 0.01 M AgNO3, a pH of 9.0, and a temperature of 50°C. The validity of the developed model was verified, and the average absorbance from six experimental runs was recorded as 2.26 (A.U) with an error of 14.86%. The synthesized AgNPs were characterized using Ultraviolet–Visible (UV–Vis) Spectroscopy, Fourier Transform Infrared (FT–IR) Spectroscopy, and Scanning Electron Microscopy–Energy Dispersive X-ray Spectroscopy (SEM–EDS). In addition, in vitro trials revealed that the synthesized AgNPs exhibited antifungal activity against all five fungal kiwifruit pathogens tested. The EC50 values of synthesized AgNPs were 10.88, 9.30, 7.15, 25.16, and 53.77 µg/ml for Phytopythium vexans, Globisoprangium sylvaticum, G. intermedium, Phytophthora citrophthora, and Rhizoctonia solani, respectively. With the exception of the MIC values of two Globisporangium species (120 µg/ml), both MIC and MFC values of the remaining three species were found to be above 150 µg/ml. The results of this study indicate that AgNPs synthesized with cherry laurel leaf extract should be further investigated for use in the control of fungal root and stem rot diseases in kiwifruit.
Keywords: Prunus laurocerasus, silver nanoparticle, Box-Behnken design, antifungal activity
dergipark.org.tr/tr/download/article-file/2622940
Antifungal activity and optimization procedure of silver nanoparticles green synthesized with Prunus laurocerasus L. (cherry laurel) leaf extract
Authors: Ugur Yigit, Yaren Gurel, Hasan Ilhan, Muharrem Turkkan
Abstract: In this study, green synthesis conditions of silver nanoparticles (AgNPs) synthesized with Prunus laurocerasus (cherry laurel) leaf extract as reducing and coating agent were optimized using Box-Behnken design (BBD). Three important synthesis factors such as the concentration (M) of silver nitrate (AgNO3), pH of cherry laurel leaf extract, and reaction temperature (°C) were used as independent variables of the model, and the absorbance intensity originating from AgNPs was employed as a dependent variable. Statistical analyzes showed that the optimized conditions for the predicted absorbance at 405 nm (2.35 A.U) were determined at a concentration of 0.01 M AgNO3, a pH of 9.0, and a temperature of 50°C. The validity of the developed model was verified, and the average absorbance from six experimental runs was recorded as 2.26 (A.U) with an error of 14.86%. The synthesized AgNPs were characterized using Ultraviolet–Visible (UV–Vis) Spectroscopy, Fourier Transform Infrared (FT–IR) Spectroscopy, and Scanning Electron Microscopy–Energy Dispersive X-ray Spectroscopy (SEM–EDS). In addition, in vitro trials revealed that the synthesized AgNPs exhibited antifungal activity against all five fungal kiwifruit pathogens tested. The EC50 values of synthesized AgNPs were 10.88, 9.30, 7.15, 25.16, and 53.77 µg/ml for Phytopythium vexans, Globisoprangium sylvaticum, G. intermedium, Phytophthora citrophthora, and Rhizoctonia solani, respectively. With the exception of the MIC values of two Globisporangium species (120 µg/ml), both MIC and MFC values of the remaining three species were found to be above 150 µg/ml. The results of this study indicate that AgNPs synthesized with cherry laurel leaf extract should be further investigated for use in the control of fungal root and stem rot diseases in kiwifruit.
Keywords: Prunus laurocerasus, silver nanoparticle, Box-Behnken design, antifungal activity
dergipark.org.tr/tr/download/article-file/2622940