International Journal of Life Sciences and Biotech
Discrimination of two species (Androctonus crassicauda and Leiurus abdullahbayrami; Buthidae Scorpions) by MALDI-TOF MS-based PCA
Authors: Yasemin Numanoğlu Çevik1, Mehmet Ali Kanat
Abstract: The venoms of the scorpions Androctonus crassicauda and Leiurus abdullahbayrami, scorpion species each of the two members of the Buthidae family, were analyzed by MALDI–TOF MS in a mass range between 1 and 50 kDa. For this, all of the scorpion venoms (n=11) were prepared to equal 2mg/mL concentration. After centrifuging the venoms at 15.000 rpm for 15 minutes at +4 ºC, the supernatants (n=11) were mixed with a matrix solution (α-CHCA) in Eppendorf tubes separately. The prepared scorpion venom-matrix (SVMx) samples vortexed. For the biomass analysis, a 1µL SVMx sample was spotted onto MALDI 96 MSP was placed in the Microflex MALDI-TOF MS. The system was operated in linear positive ion mode at a 1.000-50.000 Dalton (Da) mass range. A 60 Hz nitrogen laser was employed at 337 nm as the ion source. Interspecies differentiation was evaluated over peptide and protein molecules in this mass range. The similarities and differences between two different scorpion species were revealed with the principal component analysis study, which was conducted with spectral patterns including peptide and protein profiles. The similarity rate of the LAB-123 and the LAB460 scorpion venoms of the same species was found as 66% while the similarity rates of venoms of the ACR species to the LAB species ranged from zero to 37%. It was demonstrated that scorpion venoms belonging to two different species from the Buthidae family can be differentiated with the help of dendrogram and gel profile, CCI color matrix, 3D or 2Dscattering profile, spectral mass loading data formed by peptide and protein spectral patterns of eleven scorpion venoms. It is anticipated that this approach, which was used for the first time with the application of MALDI-TOF MS-based PCA analysis for the differentiation of scorpion venoms, will be useful in differentiating venoms with different spectral patterns.
Keywords: Androctonus crassicauda, Leiurus abdullahbayrami, Scorpion, Buthidae, MALDI-TOF MSPCA analyse
dergipark.org.tr/tr/download/article-file/2268429
Discrimination of two species (Androctonus crassicauda and Leiurus abdullahbayrami; Buthidae Scorpions) by MALDI-TOF MS-based PCA
Authors: Yasemin Numanoğlu Çevik1, Mehmet Ali Kanat
Abstract: The venoms of the scorpions Androctonus crassicauda and Leiurus abdullahbayrami, scorpion species each of the two members of the Buthidae family, were analyzed by MALDI–TOF MS in a mass range between 1 and 50 kDa. For this, all of the scorpion venoms (n=11) were prepared to equal 2mg/mL concentration. After centrifuging the venoms at 15.000 rpm for 15 minutes at +4 ºC, the supernatants (n=11) were mixed with a matrix solution (α-CHCA) in Eppendorf tubes separately. The prepared scorpion venom-matrix (SVMx) samples vortexed. For the biomass analysis, a 1µL SVMx sample was spotted onto MALDI 96 MSP was placed in the Microflex MALDI-TOF MS. The system was operated in linear positive ion mode at a 1.000-50.000 Dalton (Da) mass range. A 60 Hz nitrogen laser was employed at 337 nm as the ion source. Interspecies differentiation was evaluated over peptide and protein molecules in this mass range. The similarities and differences between two different scorpion species were revealed with the principal component analysis study, which was conducted with spectral patterns including peptide and protein profiles. The similarity rate of the LAB-123 and the LAB460 scorpion venoms of the same species was found as 66% while the similarity rates of venoms of the ACR species to the LAB species ranged from zero to 37%. It was demonstrated that scorpion venoms belonging to two different species from the Buthidae family can be differentiated with the help of dendrogram and gel profile, CCI color matrix, 3D or 2Dscattering profile, spectral mass loading data formed by peptide and protein spectral patterns of eleven scorpion venoms. It is anticipated that this approach, which was used for the first time with the application of MALDI-TOF MS-based PCA analysis for the differentiation of scorpion venoms, will be useful in differentiating venoms with different spectral patterns.
Keywords: Androctonus crassicauda, Leiurus abdullahbayrami, Scorpion, Buthidae, MALDI-TOF MSPCA analyse
dergipark.org.tr/tr/download/article-file/2268429