International Journal of Life Sciences and Biotech
Characterization of Omega-3 and Omega-6 Fatty Acid Accumulation in Chlorococcum novae-angliae Microalgae Grown under Various Culture Conditions
Authors: Elifcan Caliskan, Berat Z. Haznedaroglu
Abstract: Chlorococcum novae-angliae is a terrestrial green microalgae species with remarkable potential to synthesize omega-3 (ω-3) and omega-6 (ω-6) fatty acids. In this study, Chlorococcum novaeangliae has been subjected to varying growth conditions (light, nitrogen, salinity, and temperature) to investigate the accumulation of ω-3 and ω-6 fatty acids. Among tested growth conditions, eicosapentaenoic acid, α-linoleic acid, γ-linoleic acid, and arachidonic acid were enhanced by nitrogen limitation. Significant increases were observed in concentration of linoleic acid, an essential precursor molecule for the production ω-6 fatty acids under decreased nitrogen concentrations. Despite the lowest biomass growth, monounsaturated fatty acids and docosahexaenoic acid were increased by 14.4% and 8.7% under low light intensities, respectively. Meanwhile, the highest concentrations of palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (18:1cis-9) were also detected under nitrogen limitation. Total accumulation of ω-3 fatty acids was highest in the control group, followed by nitrogen limitation, whereas total ω-6 fatty acid accumulation was highest under nitrogen limitation followed by the control group. Total lowest fatty acid concentrations were obtained under increased salinity while low temperature conditions heavily inhibited cellular growth.
Keywords: Chlorococcum novae-angliae, essential fatty acids, microalgal lipids, omega fatty acids
dergipark.org.tr/tr/download/article-file/2286931
Characterization of Omega-3 and Omega-6 Fatty Acid Accumulation in Chlorococcum novae-angliae Microalgae Grown under Various Culture Conditions
Authors: Elifcan Caliskan, Berat Z. Haznedaroglu
Abstract: Chlorococcum novae-angliae is a terrestrial green microalgae species with remarkable potential to synthesize omega-3 (ω-3) and omega-6 (ω-6) fatty acids. In this study, Chlorococcum novaeangliae has been subjected to varying growth conditions (light, nitrogen, salinity, and temperature) to investigate the accumulation of ω-3 and ω-6 fatty acids. Among tested growth conditions, eicosapentaenoic acid, α-linoleic acid, γ-linoleic acid, and arachidonic acid were enhanced by nitrogen limitation. Significant increases were observed in concentration of linoleic acid, an essential precursor molecule for the production ω-6 fatty acids under decreased nitrogen concentrations. Despite the lowest biomass growth, monounsaturated fatty acids and docosahexaenoic acid were increased by 14.4% and 8.7% under low light intensities, respectively. Meanwhile, the highest concentrations of palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (18:1cis-9) were also detected under nitrogen limitation. Total accumulation of ω-3 fatty acids was highest in the control group, followed by nitrogen limitation, whereas total ω-6 fatty acid accumulation was highest under nitrogen limitation followed by the control group. Total lowest fatty acid concentrations were obtained under increased salinity while low temperature conditions heavily inhibited cellular growth.
Keywords: Chlorococcum novae-angliae, essential fatty acids, microalgal lipids, omega fatty acids
dergipark.org.tr/tr/download/article-file/2286931