International Journal of Life Sciences and Biotech
Investigation of the Effect of Magnesium Restriction on Glucose Transport in Schizosaccharomyces pombe
Authors: Gülşen Uz, Tuğba Pesen, Ahsen Berber, Cenk Kığ, Bedia Palabıyık, Ayşegül Topal Sarıkaya
Abstract: Magnesium is vital for many biological processes such as energy metabolism, nucleic acid, and protein synthesis, signal transduction, and cell division. Impairment of magnesium homeostasis is associated with many diseases, especially cardiovascular diseases, hypertension, type 2 diabetes, and cancer. More than 300 million people worldwide struggle with type 2 diabetes, and this number is growing exponentially. Clinical studies have shown that serum magnesium levels were decreased in patients with type 2 diabetes and that magnesium supplementation has positive effects on glucose metabolism. In this study, glucose consumption and the expression levels of glucose transporters (ght1, ght2, ght5) were investigated in magnesium transportrestricted mutant strain of Schizosaccharomyces pombe yeast, which is similar to mammalian cells in terms of biological processes and genetic mechanisms. In mutant strain with limited magnesium transport, glucose consumption increased due to the increase in magnesium added to the medium. The expression level of ght1, ght2, one of the glucose transporters, increased in 30 mM Mg2+ supplemented medium, decreased at higher magnesium concentration (75 mM), where optimum growth was observed for the strain, and there was no significant change in the expression level of ght5. Our findings indicate that the glucose transporters ght1 and ght2 are regulated by a different mechanism than ght5.
Keywords: magnesium deficiency, glucose transport, S. pombe
dergipark.org.tr/tr/download/article-file/2375284
Investigation of the Effect of Magnesium Restriction on Glucose Transport in Schizosaccharomyces pombe
Authors: Gülşen Uz, Tuğba Pesen, Ahsen Berber, Cenk Kığ, Bedia Palabıyık, Ayşegül Topal Sarıkaya
Abstract: Magnesium is vital for many biological processes such as energy metabolism, nucleic acid, and protein synthesis, signal transduction, and cell division. Impairment of magnesium homeostasis is associated with many diseases, especially cardiovascular diseases, hypertension, type 2 diabetes, and cancer. More than 300 million people worldwide struggle with type 2 diabetes, and this number is growing exponentially. Clinical studies have shown that serum magnesium levels were decreased in patients with type 2 diabetes and that magnesium supplementation has positive effects on glucose metabolism. In this study, glucose consumption and the expression levels of glucose transporters (ght1, ght2, ght5) were investigated in magnesium transportrestricted mutant strain of Schizosaccharomyces pombe yeast, which is similar to mammalian cells in terms of biological processes and genetic mechanisms. In mutant strain with limited magnesium transport, glucose consumption increased due to the increase in magnesium added to the medium. The expression level of ght1, ght2, one of the glucose transporters, increased in 30 mM Mg2+ supplemented medium, decreased at higher magnesium concentration (75 mM), where optimum growth was observed for the strain, and there was no significant change in the expression level of ght5. Our findings indicate that the glucose transporters ght1 and ght2 are regulated by a different mechanism than ght5.
Keywords: magnesium deficiency, glucose transport, S. pombe
dergipark.org.tr/tr/download/article-file/2375284