International Journal of Life Sciences and Biotech
Alpha S1-Casein Gene Polymorphism in Yankasa Sheep Breed of Mubi, Adamawa State, Nigeria
Authors: Ismaila Yada Sudi, Mohammed Shuaibu, Malachi Albert Tizhe, Augustine Clement
Abstract: The present study was conducted on five yankasa breeds of sheep to determine alpha S1-CSNISI casein gene polymorphism. Five blood samples were collected using sterile needle and syringe into sample tubes containing (EDTA, placed on ice in the cold-chain container and immediately transported to the laboratory for analysis. DNA extraction was carried out using Quick-DNA Miniprep TM kit. Primers were used to amplify the DNA fragment of interest. Nucleotide sequences were subjected to the BLAST search against the NCBI database and a similar sequence NC-0040257.1 was retrieved for comparison. Single nucleotide polymorphism was analysed for variations. Multiple sequence alignment was performed using Clustal W and reconstructed using Multalin. A MEGAx Program incorporated in GenAleX 6.503 was used to construct a phylogenetic tree and multiple sequence alignment regenerated in the Muscle (3.8) to construct phylogenetic tree within as well as with the reference (NC-040257.1). Results from the analysis revealed that the extracted DNA was found on chromosome 6, intron 16 and exon 17. The breeds showed a total number of polymorphic and monomorphic site of 68 and 600 respectively, and percentage polymorphism of 10.18%. High frequency of 0.733 was observed at position 201. Amino acid substitution in yankasa sheep breed was 4 at positions 161, 163, 164 and 201, showing the existence of variation in yankasa species possibly indicating long term chances of survival. CSN1S1 isolated in yankasa sheep showed polymorphism, genetic variation within and between breeds. Present study have revealed polymorphisms in milk proteins with high frequency (0.733) in variant at position 201Thr > Ile. In further studies, it is therefore of interest to characterize, genotype and find the allele frequencies of casein gene of yankasa sheep breed, provide a complete picture about milk protein gene and milk protein variation to improve consumer preference.
Keywords: Alpha S1-casein gene, milk protein, Mubi, polymorphism, yankasa sheep breeds
dergipark.org.tr/tr/download/article-file/1111911
Alpha S1-Casein Gene Polymorphism in Yankasa Sheep Breed of Mubi, Adamawa State, Nigeria
Authors: Ismaila Yada Sudi, Mohammed Shuaibu, Malachi Albert Tizhe, Augustine Clement
Abstract: The present study was conducted on five yankasa breeds of sheep to determine alpha S1-CSNISI casein gene polymorphism. Five blood samples were collected using sterile needle and syringe into sample tubes containing (EDTA, placed on ice in the cold-chain container and immediately transported to the laboratory for analysis. DNA extraction was carried out using Quick-DNA Miniprep TM kit. Primers were used to amplify the DNA fragment of interest. Nucleotide sequences were subjected to the BLAST search against the NCBI database and a similar sequence NC-0040257.1 was retrieved for comparison. Single nucleotide polymorphism was analysed for variations. Multiple sequence alignment was performed using Clustal W and reconstructed using Multalin. A MEGAx Program incorporated in GenAleX 6.503 was used to construct a phylogenetic tree and multiple sequence alignment regenerated in the Muscle (3.8) to construct phylogenetic tree within as well as with the reference (NC-040257.1). Results from the analysis revealed that the extracted DNA was found on chromosome 6, intron 16 and exon 17. The breeds showed a total number of polymorphic and monomorphic site of 68 and 600 respectively, and percentage polymorphism of 10.18%. High frequency of 0.733 was observed at position 201. Amino acid substitution in yankasa sheep breed was 4 at positions 161, 163, 164 and 201, showing the existence of variation in yankasa species possibly indicating long term chances of survival. CSN1S1 isolated in yankasa sheep showed polymorphism, genetic variation within and between breeds. Present study have revealed polymorphisms in milk proteins with high frequency (0.733) in variant at position 201Thr > Ile. In further studies, it is therefore of interest to characterize, genotype and find the allele frequencies of casein gene of yankasa sheep breed, provide a complete picture about milk protein gene and milk protein variation to improve consumer preference.
Keywords: Alpha S1-casein gene, milk protein, Mubi, polymorphism, yankasa sheep breeds
dergipark.org.tr/tr/download/article-file/1111911