Protein Purification

Protein Purification Traditionally protein purification methods have often resulted in the separation of protein complexes for the sake of "purity" or have inadvertently destroyed complexes as a result of the chemical or physical basis of the separation procedure. Sometimes the complex can be reconstituted by combining individually purified proteins, but in many cases activity cannot be recovered. In addition it is often difficult to identify the binding partners once they are physically separated.

 

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