Chemistry Net
normal_phase_chrom
The mechanism of retention of the solute Ph-OH is shown in a typical normal phase chromatography separation. An equilibrium is established between the charged and the protonated form of the solute. The charged form binds to the surface of the polar stationary phase and competes for the same positions with solvent molecules. If the polarity of the solvent is increased then more solvent molecules bind to the surface of the stationary phase and the solute (PhOH) elutes faster since it remains relatively unretained.
normal_phase_chrom
The mechanism of retention of the solute Ph-OH is shown in a typical normal phase chromatography separation. An equilibrium is established between the charged and the protonated form of the solute. The charged form binds to the surface of the polar stationary phase and competes for the same positions with solvent molecules. If the polarity of the solvent is increased then more solvent molecules bind to the surface of the stationary phase and the solute (PhOH) elutes faster since it remains relatively unretained.