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Longitudinal section of root tip of Thale Cress (Arabidopsis thaliana)

The image shows a longitudinal section of a root tip of Thale Cress (Arabidopsis thaliana).

 

GFP targeted to endoplasmic reticulum (ER) is expressed in the endodermis and cortex.

 

In the root, the cortex comprises the middle layers of tissues between the inner vascular core and the outer epidermis. The endodermis is the innermost cell layer of the cortex. When it is fully developed, the endodermis has a major function in the control of water and ion movements into the plant. In this image, the inner cylinder of cells shown by GFP will differentiate into the endodermis. The outer cylinder, also highlighted with GFP, will differentiate into the cells of the rest of the cortex, indicating the key role of the endodermis in the physiology of the plant.

 

You can relate this image to the ‘plan’ in the image of 'Longitudinal section of root tip of Thale Cress' and link it with images of 'Dividing cells in root tip, highlighted by GFP', 'Vascular tissue in root tip, highlighted by GFP' and 'Protophloem in root tip, highlighted by GFP', which form part of a series.

 

This image was obtained by staining with propidium iodide to show up cell walls and viewing under a confocal microscope. Green Fluorescent Protein (GFP) techniques were used to highlight certain cells in green.

 

The length of the section is approximately 150 µm.

 

Image by J. Hasseloff

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Uploaded on April 19, 2012
Taken on April 19, 2012