Microscope Contrast techniques H

I have had an idea to take photographs of the same diatom slide (a small arrangement) using various contrast techniques for comparison.

 

The photos are labelled A-K. I know what each was taken with. Techniques (in no particular order!) include;

 

dark-ground

phase contrast

Hoffman Modulation Contrast (HMC) (DIY)

DIC

oblique

bright-field

relief contrast (Leica, I think designed for inverted)

variable amplitude contrast (VAC)

 

All were taken with a x10 Plan Apo objective, apart from a couple eg. Phase which is a x10 Plan Fluor

 

All are stacked images of 7-11 individual images, and processing is minimal, and broadly the same.

Done