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Biuret Test

A characteristic purple color of biuret test, produced by adding a drop or 2 of copper sulfate solution to biuret in caustic soda solution. Biuret is formed when urea is gently heated in which ammonia is also evolved. This color test confirms the presence of a peptide link, -CO-NH-.

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Uploaded on October 6, 2007
Taken on December 10, 2006