BioTechniques
YGFP: a spectral variant of GFP. Figure 2. Bleaching properties of fluorescent proteins.
Photomicrographs of E. coli cells with a pMT1 parS sequence inserted at the origin of replication between the asnA and asnC genes. The four strains carried four different plasmids with the genes for GFP-mut2, YGFP, GFP-mut3, and YFPs fused to the pMT1 parB gene (see Supplementary Figure S4 for plasmid structure). All genes were E. coli codon-optimized and controlled by an arabinose promoter. Cells in logarithmic growth were induced with 0.2% arabinose for 30 min and kept on ice until pictures were taken. At this induction level, excess fluorescent fusion protein is synthesized and binds nonspecifically to the nucleoid in addition to the specific binding to the parS sequence in the origin. All pictures are 0.99-s exposures of bacteria excited for the periods indicated in seconds on the individual pictures.
www.biotechniques.com/BiotechniquesJournal/2011/June/YGFP...
YGFP: a spectral variant of GFP. Figure 2. Bleaching properties of fluorescent proteins.
Photomicrographs of E. coli cells with a pMT1 parS sequence inserted at the origin of replication between the asnA and asnC genes. The four strains carried four different plasmids with the genes for GFP-mut2, YGFP, GFP-mut3, and YFPs fused to the pMT1 parB gene (see Supplementary Figure S4 for plasmid structure). All genes were E. coli codon-optimized and controlled by an arabinose promoter. Cells in logarithmic growth were induced with 0.2% arabinose for 30 min and kept on ice until pictures were taken. At this induction level, excess fluorescent fusion protein is synthesized and binds nonspecifically to the nucleoid in addition to the specific binding to the parS sequence in the origin. All pictures are 0.99-s exposures of bacteria excited for the periods indicated in seconds on the individual pictures.
www.biotechniques.com/BiotechniquesJournal/2011/June/YGFP...