isev2
SCSR1
"Visualization of MSC-derived RNA transfer using the CROSS-FIRE system"
This is an image of a 5-day co-culture of HEK293T reporter cells (red) expressing Cas9 and a fluorescent Stoplight reporter alongside immortalized mesenchymal stem cells (hTERT-MSCs; gray) that express a sgRNA that targets the fluorescent Stoplight reporter. Transfer of sgRNAs from the MSC donor cells to the HEK293T reporter cells results in a Cas9-mediated genomic frameshift that permanently activates eGFP expression (green). As such, the green cells in this image represent HEK293T cells that have received RNA from MSC cells. As this intact sgRNA molecule is present in EVs, and as blocking EV release in donor cells and EV uptake in reporter cells inhibits RNA transfer in this assay, we hypothesize that this transfer is, at least in part, EV-mediated.
SCSR1
"Visualization of MSC-derived RNA transfer using the CROSS-FIRE system"
This is an image of a 5-day co-culture of HEK293T reporter cells (red) expressing Cas9 and a fluorescent Stoplight reporter alongside immortalized mesenchymal stem cells (hTERT-MSCs; gray) that express a sgRNA that targets the fluorescent Stoplight reporter. Transfer of sgRNAs from the MSC donor cells to the HEK293T reporter cells results in a Cas9-mediated genomic frameshift that permanently activates eGFP expression (green). As such, the green cells in this image represent HEK293T cells that have received RNA from MSC cells. As this intact sgRNA molecule is present in EVs, and as blocking EV release in donor cells and EV uptake in reporter cells inhibits RNA transfer in this assay, we hypothesize that this transfer is, at least in part, EV-mediated.